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Impact of Curcuma mangga Val. Rhizome Essential Oil to p53, Bcl-2, H-Ras and Caspase-9 expression of Myeloma Cell Line Astuti, Endang; Sunarminingsih, Retno; Jenie, Umar Anggara; Mubarika, Sofia; S, Sismindari
Indonesian Journal of Biotechnology Vol 19, No 1 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (492.993 KB)

Cancer is a disease, a public health problem, which is found in the world as well as in Indonesia. Ingeneral, some of cancer theraphies are ineffective, characterized by the resistance performance of cancer cell line,the exposed normal cell and by the side effects. Nowadays, studies to fi nd the specifi c and safely anti-cancerdrugs were increased by the time. Several studies revealed that Curcuma mangga Val. Rhizome contains somesecondary metabolites, essential or non-essential oil, which has cytotoxic activities to the cancer cells. Basedon these anti-cancer potentials, this study has several aims to recognize anti-cancer selectivity and molecularmechanism by inducting apoptosis and inhibiting myeloma cell proliferation. To C. mangga Val. essential oil,immunocyto chemical test was performed to determine the expression of p53, caspase-9, Bcl-2, H-Ras proteinwhile TUNEL test was performed to determine the number of apoptosis cells.The results of this study shown that anti-cancer molecular mechanism of C. mangga Val. essential oil tomyeloma cell line was performed by increasing apoptosis; by increasing the expression of pro-apoptosis p53,caspase-9 protein and reducing protein which is increasing proliferation Bcl-2 and H-Ras.

Impact of Curcuma mangga Val. Rhizome Essential Oil to p53, Bcl-2, H-Ras and Caspase-9 expression of Myeloma Cell Line Endang Astuti; Retno Sunarminingsih; Umar Anggara Jenie; Sofia Mubarika; S. Sismindari
Indonesian Journal of Biotechnology Vol 19, No 1 (2014)
Publisher : Universitas Gadjah Mada

Cancer is a disease, a public health problem, which is found in the world as well as in Indonesia. Ingeneral, some of cancer theraphies are ineffective, characterized by the resistance performance of cancer cell line,the exposed normal cell and by the side effects. Nowadays, studies to fi nd the specifi c and safely anti-cancerdrugs were increased by the time. Several studies revealed that Curcuma mangga Val. Rhizome contains somesecondary metabolites, essential or non-essential oil, which has cytotoxic activities to the cancer cells. Basedon these anti-cancer potentials, this study has several aims to recognize anti-cancer selectivity and molecularmechanism by inducting apoptosis and inhibiting myeloma cell proliferation. To C. mangga Val. essential oil,immunocyto chemical test was performed to determine the expression of p53, caspase-9, Bcl-2, H-Ras proteinwhile TUNEL test was performed to determine the number of apoptosis cells.The results of this study shown that anti-cancer molecular mechanism of C. mangga Val. essential oil tomyeloma cell line was performed by increasing apoptosis; by increasing the expression of pro-apoptosis p53,caspase-9 protein and reducing protein which is increasing proliferation Bcl-2 and H-Ras.

Synergistic effects of para-hydroxy meta-methoxy chalcone (pHmMC)- doxorubicin treatments on T47D breast cancer cells Retno Arianingrum; Retno Sunarminingsih; Edy Meiyanto; Sofia Mubarika
Indonesian Journal of Biotechnology Vol 20, No 2 (2015)
Publisher : Universitas Gadjah Mada

Resistance to some cancer chemotherapeutic drugs has been identifed. One strategy to overcome that problem is by combining two or more of the drugs to get co-chemotherapeutic effects. A derivate chalcone, 3 - (4’-hydroxy-3’-methoxyphenyl)-1-phenyl-2-propene-1-on or para hydroxy meta methoxy chalcone (pHmMC), has been reported to have cytotoxic activity on some cancer cells through some pathways. The aim of this study was to investigate the effects of combinations of pHmMC and Doxorubicin (Dox) on the cytotoxicity, anti-proliferation, apoptosis, and the cell cycle of T47D (breast cancer cell-lines) in vitro. The cytotoxic and antiproliferative activity were determined by MTT (3-[4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide] assay. The combination index (CI) was used to determine the synergistic, additive or antagonistic effects of the combinations. Flowcytometry method was performed to determine the combination effects on the apoptosis and cell cycle. The results indicated that the combinations had a higher inhibitory effect on the cell growth compared to those of single treatments of pHmMC and Dox. All the doses used in the combinations were lower of the single doses at their IC50s. The results showed all the combinations gave synergistic (CI: 0.3 – 0.7) up to strong synergistic (CI: 0.1 – 0.3) effects. The synergistic effects of the combinations were due to increased apoptosis and induced cell cycle arrest in S and G2/M phases on the cancer cell lines.

PEMUPUKAN DAN INDUKSI Curcuma mangga Val. UNTUK PENINGKATAN ZAT ANTIKANKER DAN UJI SITOTOKSITASNYA PADA T47D Retno Sunarminingsih Sudibyo; Taryono Taryono
Jurnal Penelitian Saintek Vol 25, No 1 (2020)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Penelitian ini bertujuan untuk meningkatkan zat antikanker dan uji sitotoksitasnya pada T47D dengan menggunakan metode pemupukan dan induksi pada tanaman Curcuma mangga Val. Penelitian dilaksanakan di Greenhouse milik Pusat Inovasi Agroteknologi Terpadu (PIAT) Universitas Gadjah Mada di Kalitirto, Kecamatan Berbah, Sleman, Yogyakarta. Waktu pelaksanaan awal Januari-Oktober 2019. Pelaksanaan penelitian dibagi menjadi 3 tahap. Tahap pertama penanaman dan pemupukan C. mangga Val. Pemupukan dilakukan menggunakan nitrogren (N) organik dari kompos daun-daun legume, fosfor (P) organik dari pupuk SP36, Kalium (K) dari KCl, dan kombinasi pupuk NPK. Tahap kedua adalah ekstraksi dan penetapan kandungan zat aktif menggunakan alat vaccum-rotavapor dan densitometer. Tahap ketigaadalah Uji sitotoksisitas (MTT Assay) menggunakan bahan Sel T47D, media RPMI, MTT, PBS, SDS, dan bahan-bahan disposable, serta ELISA reader. Hasil penelitian menunjukan bahwa pemupukan N organik pada C. mangga Val. mampu meningkatkan produksi minyak dalam rimpang dan sitotoksisitas minyak terhadap sel kanker payudara T47D. Penghentian pemberian air menjelang masa dorman dan suhu lingkungan yang panas (37oC) mampu menginduksimajunya masa dorman pada tanaman C. mangga Val.INCREASING ANTICANKER SUBSTANCES AND THE Cytotoxicity TEST ON T47DUSING FERTILIZATION AND INDUCTION ON Curcuma Mango Val.This study was aimed at increasing the anticancer agent and conducting a cytotoxicity test on T47D by using fertilization and induction methods on Curcuma Val mango plant. The study was conducted at the Greenhouse of Agro-technology Innovation Center (AIC), Gadjah Mada University in Kalitirto, Berbah, Sleman, Yogyakarta. The study was conducted from January to October 2019. The research was divided into 3 stages. The fi rst stage of planting and fertilizing C. mango Val. Fertilization was carried out using organic nitrogren (N) fromcompost of legume leaves, organic phosphorus (P) from SP36 fertilizer, Potassium (K) from KCl and NPK fertilizer combination. The second step is the extraction and the determination of the active ingredient using a vacuum-rotary evaporator and a densitometer. The third stage is the cytotoxicity test (MTT Assay) using T47D cell material, RPMI media, MTT, PBS, SDS and disposable materials, and ELISA reader. The results showed that organic N fertilizationon C. mango Val. able to increase oil production in rhizomes and cytotoxicity of oil against T47D breast cancer cells. Discontinuation of water delivery prior to dormancy and hot ambient temperature ( 37oC) is able to induce the advancement of dormancy in C. mango Val plants.

EFEK SITOTOKSIK BEBERAPA AKAR BAJAKAH KALIMANTAN TERHADAP SEL KANKER PAYUDARA T47D Risa Umari Yuli Aliviyanti; Retno Sunarminingsih Sudibyo; Retno Murwanti
Jurnal Penelitian Saintek Vol 26, No 2 (2021)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v26i2.41211

Indonesia memiliki sekitar 200 jenis bajakah; empat di antaranya yaitu bajakah tampala, kalalawit, bajakah jari lima, dan longkur telah digunakan oleh suku Dayak untuk pengobatan kanker payudara. Studi ini menguji sitotoksisitas antikanker payudara ekstrak akar dari keempat bajakah tersebut. Ekstraksi menggunakan sample grinding pestle dengan pelarut air, air panas, etanol 96%, dan n-heksan. Uji sitotoksisitas ekstrak dilakukan secara in vitro terhadap sel T47D dengan pembanding tamoksifen. Nilai IC50 dari uji sitotoksik ekstrak etanol, air panas, air, dan n-heksan berturut-turut: 1136; 1871,5; 2294,25; dan 3975,5 µg/mL (R2 = 0,962; 0,943; 0,943; dan 0,956), tamoksifen 9 µg/mL (R2 = 0,9581). Ekstrak etanol bajakah merupakan yang paling toksik dibandingkan dengan yang lainnya sehingga untuk perbaikan nilai IC50 dilakukan ekstraksi reflux menggunakan etanol 96% pada keempat akar bajakah. Hasil menunjukkan nilai IC50 bajakah kalalawit, tampala, longkur, dan jari lima yang membaik yaitu berturut-turut adalah 407; 708; 881; dan 1.096 µg/mL (R2 = 0,9717; 0,952; 0,9367; 0,9369). Keempat ekstrak bajakah mengandung zat aktif antikanker payudara (mengingat nilai R2 uji sitotoksisitas 0,93).CYTOTOXIC EFFECT OF KALIMANTAN BAJAKAH ROOTS ON T47D BREAST CANCER CELLSThere are 200 kinds of bajakah in Indonesia; which bajakah tampala, kalalawit, bajakah jari lima, and longkur which have been used as herbal medicine of anti-breast cancer by Dayak ethnic. This study was to find out the cytotoxicity of four bajakah extracts toward T47D cell lines with tamoxifen as a positive control. Bajakah extracts of aqua, hot aqua, ethanol 96%, and n-hexane were carried out using a sample-grinding pestle. The IC50 resulted were 1136; 1871.5; 2294.25; and 3975.5 µg/mL (R2 = 0.962; 0.943; 0.943; and 0.956) respectively for aqua, hot aqua, ethanol 96%, and n-hexane extracts; 9 µg/mL (R2 = 0.9581) for tamoxifen. The ethanolic extract was the most toxic compared to other extracts. Therefore, to improve the IC50, reflux extraction was conducted using ethanol 96% to four bajakahs. The improved IC50 were 407; 708; 881; and 1096 µg/mL (R2 = 0.9717; 0.952; 0.9367; 0.9369) for respectively bajakah kalalawit, tampala, longkur, and jari lima. It is concluded that the extracts contained active breast-anticancer substances because all the R2 results were 0.93.

MOLECULAR DOCKING OF D6-ANHYDROERYTHROMYCIN TO rRNA 23S Deinococcus radiodurans AND THE PREDICTION OF ITS ANTIBIOTIC POTENCY Winarto Haryadi; Umar Anggara Jenie; Retno Sunarminingsih Sudibyo; Harno Dwi Pranowo; Fajar Rakhman Wibowo
Indonesian Journal of Chemistry Vol 9, No 2 (2009)
Publisher : Universitas Gadjah Mada

D6-anhidroeritromisin-A is a new derivative of erythromycin which is synthesized through biosynthetic engineering technique. The molecular docking in rRNA 23S Deinoccocus radiodurans are accomplished to determine the model and strength of binding to the target macromolecule. The molecular docking of erythromycin-A and 6-deoksieritromisin-A to the same macromolecule is used as a control. The docking result of the D6-anhidroeritromisin-A shows that it occupies the same cavity as of the experimental erythromycin-A in the same macromolecule. The binding position of D6-anhidroeritromisin-A is not exactly same as erythromycin-A and 6-deoksieritromisin-A due to the presence of D6 unsaturated double bond. However the hydroxyl group(OH) at C-6 does not have an apparent effect on the binding model to rRNA23S D. radiodurans.

PENGARUH LOKASI TUMBUH, UMUR TANAMAN DAN VARIASI JENIS DESTILASI TERHADAP KOMPOSISI SENYAWA MINYAK ATSIRI RIMPANG Curcuma mangga PRODUKSI BEBERAPA SENTRA DI YOGYAKARTA (Impact of Growing Sites, Plant Ages and Variance of Distillation Types to Curcuma) Endang Astuti; Retno Sunarminingsih; Umar Anggara Jenie; Sofia Mubarika; Sismindari Sismindari
Jurnal Manusia dan Lingkungan Vol 21, No 3 (2014): November
Publisher : Pusat Studi Lingkungan Hidup Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jml.18560

ABSTRAKMinyak atsiri rimpang Curcuma mangga telah diketahui bersifat toksik terhadap beberapa jenis sel kanker. Toksisitas minyak atsiri dipengaruhi oleh komposisi senyawa penyusunnya. Komposisi minyak atsiri dipengaruhi oleh berbagai faktor ekologi dan metode isolasi. Penelitian ini bertujuan untuk mengetahui pengaruh lokasi tumbuh, umur tanaman dan variasi jenis destilasi terhadap komposisi senyawa minyak atsiri rimpang C. mangga. Untuk mempelajari pengaruh lokasi tumbuh diambil rimpang C. mangga yang berasal dari beberapa daerah di Daerah Istimewa Yogyakarta. Pengaruh umur tanaman terhadap komposisi senyawa dalam minyak atsiri digunakan rimpang C. mangga umur 1, 2, 3, 7, 10, 11 dan 12 bulan, sedangkan jenis destilasi yang digunakan adalah destilasi uap dan uap air. Analisis komponen senyawa minyak atsiri digunakan Kromatografi Gas-Spektrometer Massa (KG-SM). Hasil penelitian menunjukkan bahwa rimpang yang berasal dari daerah dataran rendah memiliki jenis senyawa yang lebih banyak daripada dataran tinggi dan dimungkinkan karena curah hujan di dataran rendah lebih kecil daripada dataran tinggi. Minyak atsiri rimpang C. mangga optimum apabila tanaman dipanen pada saat umur 8-10 bulan dan jenis destilasi berpengaruh terhadap komposisi minyak atsiri. Namun begitu, senyawa utama minyak atsiri rimpang C. mangga untuk semua variasi perlakuan adalah sama yaitu β-osimen, β-pinen, β-mirsen dan p-sineol. ABSTRACTThe essential oil of Curcuma mangga has been known to be toxic to several cancer cells. The toxicity is influenced by the substances composition. The substances composition of the essential oil is influenced by ecological factor and isolation method. Therefore, this study have several aims to recognize the impact of growing sites, plant ages and variance of distillation types to C. mangga essential oil compositions. To study the impact of the growing sites, C. mangga samples are collected from several places in Yogyakarta. To determine the impact of substances composition of C. mangga essential oil, samples of 1, 3, 7, 10, 11 and 12 months were chosen, while for distillation method, steam and water-steam distillations were used. For C. mangga essential oil’s susbtances analysis, Gas Chromatography-Mass Spectrometer (GC-MS) was performed. The results of study shown that the land samples contain more substances variance in comparison with the planteau samples, it is probably influenced by the rainfall frequency of land areas, which is lower than planteau areas. In addition, the optimum essential oil are the 8-10 month samples and the distillation method influences to the essential oil compositions. However, all variance treatment has the same main component, i.e., β-ocimene, β-pinene, β-myrcene and p-cineole.

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