M. Isa
Laboratorium Biokimia, Fakultas Kedokteran Hewan, Universitas Syiah Kuala, Banda Aceh

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Deteksi Cemaran Escherichia Coli Pada Udang Vaname (Litopenaeus Vannamei) Di Tambak Lhoong Aceh Besar Rastina Rastina; Wahyu Eka Sari; Azhari Azhari; Yunisma Andriani Munthe; M. Isa; Zainuddin Zainuddin
JURNAL ILMIAH MAHASISWA VETERINER Vol 7, No 1 (2022): NOVEMBER-JANUARI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v7i1.23794

Abstract

ABSTRAKUdang vaname merupakan salah satu komoditas ekspor perikanan andalan Indonesia yang memiliki nilai jual tinggi. Jenis udang ini juga merupakan salah satu jenis udang yang banyak ditemukan dan dimanfaatkan di Aceh. Berdasarkan data di lapangan, udang vaname pada tambak Lhoong Aceh Besar mengalami penurunan produktvitas sehingga perlu dilakukan penanganan yang baik agar produktivitas udang meningkat. Salah satu permasalahan bagi pembudidaya udang vaname adalah adanya cemaran bakteri yang dapat menyebabkan penyakit pada udang. Tujuan penelitian ini yaitu mendeteksi cemaran bakteri Escherichia coli pada udang vaname (Litopenaeus vannamei) di tambak Lhoong Aceh Besar. Sampel penelitian berjumlah 10 ekor udang vaname. Deteksi E. coli menggunakan media Eosin Methylen Blue Agar (EMBA) dengan metode Total Plate Count (TPC), serta data hasil pengamatan dianalisis secara deskriptif. Hasil penelitian ini menunjukkan bahwa pada 10 sampel udang menunjukkan hasil negatif E. coli namun positif Coliform, karena pada media EMBA tidak terlihat adanya warna hijau metalik melainkan warna merah muda, dengan jumlah koloni terendah 4,2x10² CFU/g dan jumlah koloni tertinggi 1,3x104 CFU/g. Kesimpulan dari penelitian ini adalah tidak ditemukan adanya cemaran E. coli namun terdapat keberadaan bakteri Coliform dan melebihi batas maksimum cemaran Colifrom pada udang vaname. Dengan demikian adanya keberadaan bakteri Coliform menjadi indikator dugaan adanya bakteri patogen lainnya.Kata kunci: Litopenaeus vannamei, Escherichia coli, Coliform, udang vanameABSTRACT           Vaname shrimp is one of Indonesia's primary fishery export commodities with a high selling value. This type of shrimp is also a type of shrimp that is commonly found and used in Aceh. Based on data in the field, vannamei shrimp in Lhoong Aceh Besar ponds have decreased productivity, so proper handling is needed so that shrimp productivity increases. One of the problems for vaname shrimp cultivators is the presence of bacterial contamination that can cause disease in shrimp. This study aimed to detect Escherichia coli bacterial contamination in vannamei shrimp (Litopenaeus vannamei) in Lhoong ponds in Aceh Besar. The research sample consisted of 10 vaname shrimp. E. coli was detected using Eosin Methylene Blue Agar (EMBA) medium with the Total Plate Count (TPC) method, and the observed data were analyzed descriptively. The results of this study indicate that the 10 shrimp samples showed negative results for E. coli but positive for Coliform because, in the EMBA media, there was no metallic green color but pink color, with the lowest number of colonies 4.2x10² CFU/g and the highest number of colonies 1, 3x104 CFU/g. This study concluded that no E. coli contamination was found, but the presence of Coliform bacteria exceeded the maximum limit of Coliform contamination in vannamei shrimp. Thus, the presence of Coliform bacteria indicates the suspected presence of other pathogenic bacteria.Keyword: Litopenaeus vannamei, Escherichia coli, Coliform, vaname shrimp
The Comparison of The Death Time of Hanged and Unhanged Broilers After Slaughter Azhari Azhari; Sugito Sugito; Ferllya Adela Putri; Wahyu Eka Sari; M. Jalaluddin; Hamny Sofyan; Muttaqien Muttaqien; Rosmaidar Rosmaidar; M. Isa
Jurnal Medika Veterinaria Vol 17, No 1 (2023): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v17i1.33141

Abstract

Halal slaughter is a way to kill animals by the process of draining blood properly, perfectly, and according to the sharia. One of the critical points of halal in the slaughtering process is the timeof death of the broilers after slaughter. The objective of this study was to determine the difference between the time of death of hanged broilers and unhanged broilers, in order to find out the right time before the scalding. The samples used were 10 male broilers (Gallus domesticus) five weeks old with body weight 1.6-1.7 kg/head. The variable observed was the time of death of hangedand unhanged broilers after slaughter looking at the signs of  death,  such  as  heart  rate  stopped,  pupil or  eyelid  reflexes,  and  stopped breathing. This study used a completely randomized design (RAL) with two treatments, such as slaughter of chickens in a hanging position (P1) and chickens without hanging (P2). The test data were analyzed using the independent T-test SPSS 25. The results obtained that the average time of death of hanged broilers after slaughter was 82,80 seconds, while the average time of death of unhanged broilers was 162.60 seconds. The test results of the two treatments with a significant level of 0.05 showed a significant difference. The conclusion of this study was time of death of hanged broilers is faster than that of unhanged broilers after slaughter
4. Case Study: Gallstones (Cholelithiasis) in Long-tailed Macaques (Macaca fascicularis) J. Susilo; B. Tri Wibowo; Y. Tinasari; M. Nur Salim; Dian Masyitha; Erdiansyah Rahmi; M. Isa; Herialfian Herialfian; Fakhrurrazi Fakhrurrazi; T. Fadrial Karmil; Fadli A. Gani
Jurnal Medika Veterinaria Vol 15, No 2 (2021): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v15i2.25204

Abstract

The purpose of this case study is to diagnose the cause of death of the long-tailed macaque (Macaca fascicularis). Two cadaver of Macaca fascicularis were received by the Lampung Veterinary Center from a customer. Necropsy was carried out at the Pathology Laboratory of the Lampung Veterinary Center by observing macroscopic changes. The samples taken were then observed by gram staining in the Bacteriology laboratory and hematoxylin-eosin staining at the Pathology laboratory. The results of macroscopic observations showed an enlarged gallbladder with a length of 4 cm and a width of 2 cm, blackish in color. Gallbladder surgery found 15 gallstones measuring 2 mm in diameter, brownish-colored bile, fibrin clots, and blood. The native gram stain of bile showed several types of rod-shaped bacteria form and gram-negative cocci (eosinophilic), and gram-positive rods (basophilic). Specific histopathological changes in the ductus choleductus are multifocal neutrophil inflammatory cell infiltration, enlarged gallbladder lumen filled with multi-bacterial, cell debris, fibrin, and erythrocytes. Multifocal hepatitis occurs by neutrophils, multibacterial, and fibrin. It can be concluded that the causes of death of Macaca fascicularis were cholelithiasis and hepatitis.