Mufti Kamaruddin
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Journal : Jurnal Veteriner

Produksi dan Isolasi Protein Membran Stadium Bradizoit Toxoplasma gondii : Suatu Usaha untuk Mendapatkan Material Diagnostik dalam Mendiagnosa Toksoplasmo Muhammad Hanafiah; Wisnu Nurcahyo; Mufti Kamaruddin; Fadrial Karmil
Jurnal Veteriner Vol 10 No 3 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study was conducted to isolate the membrane protein of Toxoplasma gondii at bradyzoid stage forthe development of intradermal diagnostic test in livestock infected by the parasite. Toxoplasma wasinitially collected from meat of goat serologically positive to the parasite. The infected meat was then fedinto uninfected cat to obtain oocyst. The oocyst was inoculated into the stomach of mice to produce tachyzoitwhich in turn produce cyst in tissue known as bradyzoit . The membrane protein was then isolated from thebradyzoit. The protein was analysed by sodium dodecyl sulfate electrophoresis (SDS-PAGE). The dataobtained were presented descriptively. The protein concentration isolated from each mouse infected at thedose of 1x107 oocysts was 11.91 mg. Two protein bands specific for bradyzoit were identified at 97.72 kDaand 67.60 kDa.
INTENSITAS CEMARAN LARVA NEMATODA GASTROINTESTINAL PADA RUMPUT GEMBALAAN TERNAK DOMBA DI ACEH BESAR Mufti Kamaruddin; Muhammad Hanafiah; Nurdianti -
Jurnal Veteriner Vol 8 No 1 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Telah dilakukan penelitian tentang intensitas cemaran larva nematoda dan jenis larva yang dominan pada rumput pengembalaan ternak domba di Aceh Besar. Sampel rumput diambil dari 4 desa, yaitu Meunasah Papeun, Lamreung, Leung Ie dan Cot Leupan. Pengambilan sampel dilakukan dengan membuat plot berbentuk huruf W pada sampel rumput yang akan diperiksa.