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STUDI INFEKSI NEMATODA GASTROINTESTINAL PADA KAMBING DAN DOMBA DI RUMAH POTONG HEWAN BANDA ACEH = STUDY OF GASTROINTESTINAL NEMATODES INVESTING GOATS AND SHEEP AT THE BANDA ACEH SLAUGTHERHOUSE Muhammad Hanafiah; Winaruddin .; Rusli .
Jurnal Sain Veteriner Vol 20, No 1 (2002): Juli
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4521.346 KB) | DOI: 10.22146/jsv.389

Abstract

Telah dilakukan suatu penelitian untuk mengidentifikasi jenis-jenis parasit nematoda gastrointestinal yang menyerang kambing dan domba jantan lokal yang dipotong di Rumah Potong Hewan (RPH) Banda Aceh. Penelitian ini juga bertujuan untuk mengetahui jumlah masing-masing genus cacing yang menyerang kedua ternak tersebut. Masing-masing sebanyak 50 gastrointestinal kambing dan domba yang ada di RPH Banda Aceh ditentukan secara acak akan digunakan sebagai objek penelitian. Pemeriksaan terhadap cacing untuk keperluan identifikasi cacing nematoda gastrointestinal dilakukan setelah cacingcacing yang didapat dimasukkan ke dalam laktofenol selama 6 jam. Penentuan genus cacing nematoda gastrointestinal dilakukan sesuai dengan metode Soulsby (1982). Hasil identifikasi ternak kambing dan domba yang dipotong di RPH Banda Aceh menunjukkan bahwa kedua ternak tersebut sudah terinfestasi oleh cacing-cacing nematoda gastrointestinal. Adapun masingmasing genus untuk kambing adalah : Trichostrongylus spp, Oesophagustontum spp, Bunostomum spp, Chabertia spp, Trichuris spp dan Haemonchus spp. Sedangkan untuk domba cacing nematoda gastrointestinal dari genus: Gaigeria sp, Strongyloides spp, Bunostomum spp, Oesophagustomum spp, Haemonchus spp, Chabertia spp, dan Trichuris spp. Jumlah cacing nematoda gastrointestinal yang menyerang temak kambing sebanyak 514 ekor cacing sedangkan yang menyerang domba sebanyak 543 ekor cacing. Prilaku atau kebiasaan makan tidak signifikan (P > 0,05) terhadap jumlah cacing.nematoda gastrointestinal.
PEMERIKSAAN LEUCOCYTOZOONOSIS PADA BROILER DAN ITIK MENGGUNAKAN METODE GERUSAN ORGAN DAN HAPUSAN DARAH (EXAMINATION OF LEUCOCYTOZOONOSIS IN BROILERS AND DUCKS USING ORGAN MASHED METHODS AND BLOOD SMEAR *) Muhammad Hanafiah; Rusli Sulaiman; Nuraini Latif
Jurnal Veteriner Vol 8 No 2 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The study has been done to examine Leucocytozoonosis case using organ mashed method in broiler and ducks slaughtered in chickens slaughter house in Banda Aceh Nanggroe Aceh Darussalam. From each of broiler and ducks fifty organs were collected for sample. Before those animals were killed blood smear were taken to find merozoites and gametosites stadium while smashed organs were made to find schizont stadium. The number of schizont of Leucocytozoonosis were analyzed descriptively. Result of research indicate that was higher positiveLeucocytozoonosis with mashed method better than blood smear in broiler and duck. The prevalence of Leucocylozoonosis with blood smear respectively are 30 % and 24 % from broiler while by using organ smashed methods are 58 % and 54 % respectively.
Produksi dan Isolasi Protein Membran Stadium Bradizoit Toxoplasma gondii : Suatu Usaha untuk Mendapatkan Material Diagnostik dalam Mendiagnosa Toksoplasmo Muhammad Hanafiah; Wisnu Nurcahyo; Mufti Kamaruddin; Fadrial Karmil
Jurnal Veteriner Vol 10 No 3 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (805.57 KB)

Abstract

A study was conducted to isolate the membrane protein of Toxoplasma gondii at bradyzoid stage forthe development of intradermal diagnostic test in livestock infected by the parasite. Toxoplasma wasinitially collected from meat of goat serologically positive to the parasite. The infected meat was then fedinto uninfected cat to obtain oocyst. The oocyst was inoculated into the stomach of mice to produce tachyzoitwhich in turn produce cyst in tissue known as bradyzoit . The membrane protein was then isolated from thebradyzoit. The protein was analysed by sodium dodecyl sulfate electrophoresis (SDS-PAGE). The dataobtained were presented descriptively. The protein concentration isolated from each mouse infected at thedose of 1x107 oocysts was 11.91 mg. Two protein bands specific for bradyzoit were identified at 97.72 kDaand 67.60 kDa.
INTENSITAS CEMARAN LARVA NEMATODA GASTROINTESTINAL PADA RUMPUT GEMBALAAN TERNAK DOMBA DI ACEH BESAR Mufti Kamaruddin; Muhammad Hanafiah; Nurdianti -
Jurnal Veteriner Vol 8 No 1 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Telah dilakukan penelitian tentang intensitas cemaran larva nematoda dan jenis larva yang dominan pada rumput pengembalaan ternak domba di Aceh Besar. Sampel rumput diambil dari 4 desa, yaitu Meunasah Papeun, Lamreung, Leung Ie dan Cot Leupan. Pengambilan sampel dilakukan dengan membuat plot berbentuk huruf W pada sampel rumput yang akan diperiksa.
KEPADATAN JENTIK NYAMUK Aedes spp DI GAMPONG PEURADA, KECAMATAN SYIAH KUALA, KOTA BANDA ACEH (DENSITY OF Aedes spp LARVAE IN PEURADA VILLAGE, SYIAH KUALA SUBDISTRICT, BANDA ACEH CITY) farida athaillah; Muhammad Hanafiah; arif gumilar; yudha fahrimal; t fadrial karmil; Nuzul Asmilia
JURNAL ILMIAH MAHASISWA VETERINER Vol 3, No 4 (2019): AGUSTUS-OKTOBER
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v3i4.7236

Abstract

Penelitian ini bertujuan untuk mengetahui kepadatan jentik nyamuk Aedes spp di Gampong Peurada, Kecamatan Syiah Kuala, Kota Banda Aceh. Penelitian ini dilakukan dengan mengunakan metode single larva (survei). Wilayah survei meliputi dusun-dusun yang terdapat di Gampong Peurada yaitu Dusun Setia, Dusun Sentosa dan Dusun Bahagia dengan jumlah sampel yang diambil sebanyak 40 rumah setiap dusun, total sampel sebanyak 120 rumah. Data jentik yang diperoleh dari berbagai jenis wadah yang ditemukan di Gampong Peurada, dihitung kepadatan berdasarkan parameter indeks jentik yaitu House Index (HI), Container Index (CI) dan Bretau Index (BI). Dari hasil penelitian yang telah dilakukan didapatkan nilai Density Figure (DF) adalah 3,3 atau pada rentang (2-5) yang termasuk dalam kategori kepadatan populasi sedang. Hasil tersebut didapatkan dari nilai  indeks jentik nyamuk Aedes spp di Gampong Peurada yaitu House Index (HI) : 17%, Container Index (CI) : 10%, Bretau Index(BI) : 27. (This study was aimed to determine the density of Aedes spp larvae in Peurada Village, Syiah Kuala Subdistrict, Banda Aceh City. This research was conducted by using single larvae method (survey). The survey area includes the hamlets located in Peurada Village, Setia Hamlet; Sentosa Hamlet and Bahagia Hamlet with the number of samples taken as many 40 houses each hamlet, total sample of 120 houses. The larvae data wer obtained from the various types of containers found in Gampong Peurada, were density calculated based on larvae index parameters is House Index (HI), Container Index (CI) dan Bretau Index (BI). The results of research which have been done got value density figure is 3,3 or included in medium density category with Density Figure number (2-5). The results got based on the index larvae of Aedes spp in Gampong Peurada obtained House Index (HI): 17%, Container Index (CI): 10%, Bretau Index (BI): 27.) 
DISTRIBUSI NYAMUK Aedes spp MENGGUNAKAN OVITRAP DI KOPELMA DARUSSALAM KECAMATAN SYIAH KUALA, BANDA ACEH (DISTRIBUTION OF Aedes spp FEAR IN KOPELMA DARUSSALAM OF SYIAH KUALA SUBDISTRICT BANDA ACEH) Aza Annisa Utami; Farida Athaillah; Muhammad Hanafiah
JURNAL ILMIAH MAHASISWA VETERINER Vol 6, No 2 (2022): FEBRUARI-APRIL
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v6i2.8412

Abstract

ABSTRAKPenelitian ini bertujuan untuk mengetahui distribusi nyamuk Aedes  spp dengan menggunakan ovitrap di Kopelma Darussalam, Kecamatan Syiah Kuala  baik di dalam ruangan (indoor) maupun di luar ruangan (outdoor). Penelitian ini menggunakan metode ovitrap yang dilakukan di lima dusun di Kopelma Darussalam Kecamatan Syiah Kuala yaitu:  Dusun Sederhana, Sektor Timur, Sektor Barat, Sektor Selatan, dan Sektor Utara. Hasil penelitian memperlihatkan total rata-rata jumlah telur nyamuk Aedes spp pada indoor dan outdoor di kelima dusun  tidak memperlihatkan perberbedaan yang nyata (P0,05). Tetapi  pada pengamatan terhadap rata-rata larva Aedes agypti pada indoor dan outdoor memperlihatkan perbedaan yang  nyata (P0,05). Sedangkan hasil pengamatan pada larva Aedes albopictus pada indoor dan outdoor juga tidak memperlihatkan perbedaan yang  nyata (P0,05). ABSTRACT            This study aims to determine distribution of Aedes spp using ovitrap in Kopelma Darussalam area, Syiah Kuala subdistrict both indoor (indoor) and outdoor (outdoor). The study was conducted in five sub-village in Kopelma Darussalam, Syiah Kuala district : Dusun Sederhana, Sektor Timur, Sektor Barat, Sektor Selatan, and Sektor Utara by using ovitrap method. The result showed that the total of  Aedes spp mosquito eggs on indoor and outdoor in the five hamlets did not show a significant difference (P 0.05). But on observation of mean Aedes aegypti larvae on indoor and outdoor spaces showed significant difference (P 0,05). While, observations on Aedes albopictus larvae in both indoor and outdoor spaces did not showed significant differences (P 0.05).
Gambaran Histopatologi Toksoplasmosis pada Kucing Peliharaan (HISTOPATHOLOGICAL FEATURES OF TOXOPLASMOSIS IN DOMESTIC CAT) Muhammad Hanafiah; Wisnu Nurcahyo; Joko Prastowo; Sri Hartati
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (166.81 KB) | DOI: 10.19087/jveteriner.2017.18.1.11

Abstract

Study of histopathological changes of domestic cat organs which were serologically positive toxoplasmosis and laboratory infected which Toxoplasma have been undertaken. Histological section is prepared from organs including brain, liver, lung, kidney, duodenum, jejunum, ileum, and spleen then stained using hematoxylin and eosin (HE) and observed under microscope for histopathological changes. The results showed that in the serologically positive animals cell proliferation, infiltration of leucocyte and macrophage cells were observed in the ileum, whilst infiltration of eosinophil and leucocyte was seen in the kidney and liver. However, in other organ such as duodenum, jejunum, and spleen there were no changes observed. In cat experimentally infected with Toxoplasma, the infiltration of eosinophil cells were observed in the ileum and lung, while other organs such as kidney, liver, brain, jejunum, duodenum, and spleen showed no infiltration of inflammation cells. In conclusion, based on the results seropositive cat, showed proliferation of epithelial cells, leucocyte cells, and macrophage cells in the ileum, while in the lung, kidney, and liver showed infiltration of eosinophil and leucocyte. No infiltration of inflammation cells were observed in the brain, jejunum, duodenum, and spleen. ABSTRAK Penelitian mengenai histopatologi beberapa organ kucing peliharaan yang positif Toxoplasma baik secara serologi maupun yang diinfeksikan telah dilakukan. Tujuan penelitian adalah untuk mengetahui perubahan histopatologi pada organ kucing yang positif Toxoplasma. Data hasil pemeriksaan histopatologi yang terdapat pada preparat jaringan masing-masing organ dianalisis secara deskriptif dengan melihat gambaran perubahan histopatologi pada organ otak, hati, paru, ginjal, duodenum, jejenum, ileum, dan limpa. Hasil penelitian menunjukkan bahwa dengan menggunakan metode histopatologi organ kucing yang positif toksoplasmosis secara serologi teramati adanya proliferasi sel epitel, infiltrasi sel-sel leukosit dan makrofag pada ileum, ginjal, dan hati terlihat adanya infiltrasi eosinofil dan juga infiltrasi leukosit, sedangkan organ yang lain seperti jejenum, duodenum, dan limpa tidak teramati perubahan pada jaringan yang diperiksa. Sementara pada kucing yang dinfeksikan Toxoplasma, ileum dan paru teramati adanya infiltrasi sel-sel eosinofil, sedangkan organ lainnya seperti ginjal, hati, otak, jejenum, duodenum, dan limpa tidak teramati adanya infiltrasi sel-sel radang. Simpulan yang dapat ditarik adalah pada organ kucing yang positif toksoplasmosis teramati adanya proliferasi sel epitel, infiltrasi sel-sel leukosit, dan makrofag pada ileum, paru, ginjal dan hati teramati adanya infiltrasi eosinofil dan juga infiltrasi leukosit, sedangkan organ-organ lainnya seperti otak, jejenum, duodenum dan limpa tidak terlihat adanya infiltrasi sel-sel radang.
Insidensi Nematoda Gastrointestinal dan Protozoa pada Monyet Ekor Panjang (Macaca fascicularis) Liar di Taman Wisata Alam (TWA) Pulau Weh Sabang Erdiansyah Rahmi; Muhammad Hanafiah; Amalia Sutriana; Muhammad Hambal; Farid Wajidi
Jurnal Ilmiah Ilmu-Ilmu Peternakan Vol. 13 No. 6 (2010): Mei 2010
Publisher : Fakultas Peternakan Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (49.946 KB) | DOI: 10.22437/jiiip.v0i0.115

Abstract

The research aims to find out the  incidence of gastrointestinal parasite in wild long tail macaque in wildlife  reserve  park, Weh  Island, Sabang. For  that purpose,  fecal  specimens  from 25 wild  long  tail macaques  in Weh  Island-  Sabang were  collected.  The  fresh  faeces  samples were  then  preserved  using AFA  (alcohol-formal-acetic)  liquid  prior  to  be  examined  in  Parasitology  Laboratorium,  Faculty  of Veterinary Medicine, Syiah Kuala University.   The  presence of gastrointestinal  parasite was  observed using centrifuge method as well as Boray  sedimentation method. The obtained data were tabulated and analyzed  descriptively.  The  result  revealed  that  two  parasite  species  were  found  in  gastrointestine; Eimeria  spp.    (12%)  and  Strongloides  spp.  (8%).  It  could  be  concluded  that  the  prevalence  of gastrointestinal  parasite  in wild  long  tail macaque  in wildlife  reserve  park, Weh  Island-  Sabang was considered mild.
FAKTOR RISIKO INFEKSI Toxoplasma gondii PADA KUCING DOMESTIK YANG DIPELIHARA DI YOGYAKARTA Muhammad Hanafiah; Wisnu Nurcahyo; Joko Prastowo; Sri Hartati
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (225.963 KB) | DOI: 10.21157/j.ked.hewan.v9i1.2792

Abstract

Tujuan penelitian adalah menentukan kemungkinan faktor-faktor risiko terhadap kemunculan toksoplasmosis. Sebanyak 132 sampel serum darah diambil pada kucing lokal di Yogyakarta. Data-data epidemiologis seperti asal usul kucing, ras kucing, jenis kelamin, umur, lokasi sistem pemeliharaan, jenis pakan, dan frekuensi diare diberi kode untuk mempermudah analisis, kemudian dimasukkan, disimpan, dan dianalisis dengan program Statistix Versi 7 (Analytical Sofware inc). Analisis data dilakukan secara bivariat (Chi-square (2), dan kekuatan asosiasi (OR), dan multivariat (regresi logistik). Hasil penelitian menunjukkan bahwa faktor-faktor yang berpengaruh terhadap adanya toksoplasmosis pada kucing berdasarkan analisis bivariat adalah pembersihan kotak pasir 1 kali sehari dan mandi 2-3 kali seminggu sedangkan faktor-faktor yang memiliki peluang meningkatkan seropositif toksoplasmosis berdasarkan analisis multivariat adalah pemeliharaan kucing yang bebas di dalam rumah, dimandikan lebih dari 1 kali seminggu, dan dimandikan lebih besar dari 1 bulan sekali.
Knowledge and Attitudes of Farmer Regarding The Use of Antibiotic and Its Resistance in Broiler Chicken at Aceh Besar Farm Aulia Malik Fajar; Rastina Rastina; Muhammad Jalalluddin; Ismail Ismail; Muhammad Hanafiah; Teuku Reza Ferasyi
The International Journal of Tropical Veterinary and Biomedical Research Vol 6, No 1 (2021): Vol. 6 (1) May 2021
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (190.911 KB) | DOI: 10.21157/ijtvbr.v6i1.23868

Abstract

This study aims to assess farmers' knowledge and attitudes towards the use of antibiotics in broiler chickens in Aceh Besar Farm. The respondents' population was obtained from 13 farmers who were interviewed, and the results obtained were 13 samples with a margin of error of 5%. Data were collected by interview using an open questionnaire that had been validated. The validity test results of farmers' knowledge and attitudes have a value of r 0.5529, which means that they are declared valid. The reliability test results of knowledge and attitudes of animal husbandry have a value of 0.60, so both are declared reliable. Data were analyzed using independent t-test. Based on the interview results, 80% of the farmer respondents in Aceh Besar had good knowledge of the use of antibiotics, 93.33% of respondents gave antibiotics according to clinical symptoms. Farmers' knowledge and attitudes towards the use of antibiotics based on business experience (5 years and ≥ 5 years) showed significantly different results (p0.05). The farmer's knowledge and attitudes towards the use of antibiotics based on the number of broilers raised on the farm showed no significant difference (p0.05). Based on gender and age, farmers' knowledge and attitudes showed no significant difference (p 0.05 in all comparisons). The knowledge and attitudes of 13 farmers were good towards the use and resistance of antibiotics in Aceh Besar District.