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Mitra Slipranata
Fakultas Kedokteran Hewan, Universitas Gadjah Mada
Veterinary

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Potensi Ekstrak Daun Sage (Salvia officinalis.L) sebagai anti-Streptococcus suis Penyebab Zoonotik Meningitis Mitra Slipranata; Fajar Budi Lestari; Novra Arya Sandi; Siti Isrina Oktavia Salasia
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6908.327 KB) | DOI: 10.22146/jsv.27558

Abstract

The phenomenon of microbial resistance to an antibiotic or some kind of specific antibiotics (multi drugs resistance) greatly complicate the treatment process, one of which is Streptococcus suis (S. suis) which is known to cause meningitis in animals and humans. Today, with rising bacterial resistance to a wide rangeof antibiotics, it takes an effort to assess the potential medicinal plants as an antibiotic that is appropriate and safe. Sage (Salvia officinalis.L) is reported to have antibacterial and fungicidal effect. Phenolic acids such assalvin and ether monomethyl salvin which isolated from the sage thought to have antimicrobial activity against several bacterial strains. The purpose of this study was to prove the ability of sage leaf extract as an antibacterialagainst S. suis causes streptococcal meningitis in vitro. In-vitro method used in this research through a two-stage dilution test and the diffusion test on Mueller Hinton Agar (MHA). S. suis isolates (code 225) were tested in vitro against 8 levels sage leaf extract concentration, ie a concentration of 1%, 3% 5%, 7%, 10%, 20%, 40% and 60%. The results of the test obtained by minimum inhibitory concentrations (MICs) and minimum sage known to potentially inhibit the growth of S. suis.
Prevalensi Strain Avian Pathogenic Escherichia coli (APEC) Penyebab Kolibasilosis pada Burung Puyuh Wahyu Prihtiyantoro; Khusnan Khusnan; Mitra Slipranata; Imron Rosyidi
Jurnal Sain Veteriner Vol 37, No 1 (2019): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (902.827 KB) | DOI: 10.22146/jsv.48520

Abstract

Avian Pathogenic Escherichia coli (APEC) is a pathogen that causes colibacillosis in poultry, including salpingitis, omphalitis, cellulitis, swollen head syndrome, coligranuloma yolk sac inflammation, and air sacs inflammation. APEC is a zoonotic strain which spread through raw meat and processed meat products of animals and birds. In this research, the isolation and identification of Escherichia coli were done by using selective media MacConkey, Kligger Iron Agar, and Gram staining. Polymerase chain reaction (PCR) was used to analyse genetopically to detect 16SrRNA genes, vt1 genes, and vt2 genes. Thirty one (55,36%) isolates of 56 specimens collected from quail were detected as Escherichia coli. The detection of APEC strains towards 31 Escherichia coli isolates were done by using polymerase chain reaction (PCR) with vt1 and vt2 specific primer. The results showed that 32,26% (10/31) was APEC strains and 67.74% was non-APEC strains. From 10 isolates, 90% had vt1 gene and 10% had vt2 gene. Escherichia coli isolates were found in eyes (32,26%), infraorbital sinus fluid (32,26%), nasal fluid (16,20%), also in lungs, air sacs, ascites, and heart for 3,2% each. The isolates could not be found in the specimens from the skull. As a zoonotic agent, the isolates have an impact on human health. 
Co-Authors Fajar Budi Lestari Imron Rosyidi Khusnan k Novra Arya Sandi Siti Isrina Oktavia Salasia Wahyu Prihtiyantoro