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All Journal Jurnal Sain Veteriner Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Jurnal Bisnis dan Manajemen Wahana Peternakan Journal of Livestock and Animal Health (JLAH)

Fajar Budi Lestari

Departement Of Bioresources Technology And Veterinary, Vocational College, Universitas Gadjah Mada, Yogyakarta, Indonesia

Author-ID : 3368610

Agriculture, Biological Sciences & Forestry Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Veterinary

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Klasterisasi Staphylococcus aureus Resisten Neutrofil Berdasar Assesory Gene Regulator Santosa, Christin Marganingsih; Lestari, Fajar Budi; Widayanti, Rini; Salasia, Siti Isrina Oktavia
Jurnal Sain Veteriner Vol 38, No 2 (2020): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.50653

Staphylococcus aureus is recognized worldwide as a major pathogen causing subclinical intramammary infections in dairy cows and food poisoning due to its ability to produce enterotoxin. The study aimed to identify enterotoxins of S. aureus and clustering the enterotoxins based on assessory gene regulator (agr). Virulence of S. aureus to the host was characterized based on the response of polymorphonuclear cells to the infection. Twelve S. aureus could be isolated from milk cows in central of dairy farming in Sumedang West Java. The identification of S. aureus was based on cultural and biochemical tests and an amplification of a specific section of the 23S rRNA gene. The sensitivity test against antibiotics revealed that some isolates of S. aureus were resistant to penicillin and methycillin. By PCR amplification one or more staphylococcal enterotoxin genes could be observed five genes in combinations of sea (216 bp), seb (478 bp), seh (375 bp), sei (576 bp), and sej (142 bp). Clustering of S. aureus based on the assesory gene regulator could be grouped into 4 clusters for agr1 (1 isolat), agr2 (2 isolates), in combination for agr1 and agr2 (1 isolate), and for non agr (2 isolates). Based on the response of polymorphonuclear cell in vitro and in vivo assays, revealed that S. aureus strain I-2 (agr1 cluster) and P1 (agr1+agr2 cluster) were more resistant to polymorphonuclear cells and could survive intracellularly, indicated that these strains could be used as proper candidates to develop dignostic tool based on agr against staphylococcal mastitis.

Karakterisasi Staphylococcus aureus Isolat Susu Sapi Perah Berdasar Keberadaan Protein-A pada Media Serum Soft Agar terhadap Aktivitas Fagositosis Secara In Vitro Fajar Budi Lestari; Siti Isrina Oktavia Salasia
Jurnal Sain Veteriner Vol 33, No 2 (2015): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Staphylococcus aureus merupakan salah satu bakteri penyebab utama mastitis. Protein-A berperan penting dalam adesi dan kolonisasi bakteri pada sel inang. Penelitian ini bertujuan untuk mengetahui hubungan aktivitas fagositosis S. aureus berdasarkan keberadaan protein-A pada media serum soft agar. Sebanyak 19 isolat S. aureus susu sapi perah asal Jawa Barat dan Jawa Tengah digunakan pada penelitian ini. Seluruh isolat tersebut direidentifikasi dengan dipupuk pada media plat agar darah (PAD), koloni bakteri kemudian diidentifikasi dengan pewarnaan Gram, uji mannitol salt agar (MSA), katalase dan uji koagulase. Karakterisasi S.aureusdilakukan dengan menanam bakteri pada media serum soft agar (SSA) yang mengandung serum kelinci untuk mengetahui keberadaan protein-A, kemudian dilakukan uji fagositosis dengan menggunakan sel polimorfonuklear. Dari 19 isolat tersebut seluruhnya teridentifikasi sebagai S. aureus yang ditunjukkan dengan Gram positif, sel berbentuk kokus bergerombol, mampu memfermentasi manitol pada media MSA, positif pada uji katalase, 15,79% sampel menunjukkan hasil koagulase negatif, sedangkan 84,21% menunjukkan hasil koagulase positif. Pertumbuhan pada media SSA menunjukkan hasil 12 isolat (63,16%) koloni berbentukkompak dan 7 isolat (36,84%) koloni berbentuk difus. Koloni kompak menunjukkan bakteri tersebut memiliki protein-A, koloni difus menunjukkan bakteri tersebut tidak memiliki protein-A atau memiliki protein-A tetapi tertutup oleh kapsul. Hasil uji fagositosis menunjukkan S. aureus yang memiliki protein-A lebih sedikit difagosit oleh leukosit polimorfonuklear (2,99 bakteri/sel) dari pada S. aureus yang tidak memiliki protein-A, atau mempunyai protein-A tetapi tertutup oleh kapsul (3,85 bakteri/sel). Staphylococcus aureus yang memiliki protein-A lebih patogen daripada S. aureus yang tidak memiliki protein-A. Isolat S. aureus asal Jawa Tengah lebih virulen dibandingkan isolat S. aureus asal Jawa Barat ditinjau dari sifat hemolisis, koagulase, dan protein-A.

Deteksi Gen Penyandi Sifat Resistensi Metisilin, Penisilin dan Tetrasiklin pada Isolat Staphylococcus aureus Asal Susu Mastitis Subklinis Sapi Perah Fatkhanuddin Aziz; Fajar Budi Lestari; Sarah Nuraidah; Endah Purwati; Siti Isrina Oktavia Salasia
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Detection of gene encoding resistance of bacteria could be used as an accurate method to determine resistance of Staphylococcus aureus which is causing mastitis in dairy cows to the several antibiotics. This research aimed to detect the gene encoding resistance of methicillin, penicillin and tetracycline from identified S. aureus. Sixty milk samples were collected from subclinical mastitis of cows from various dairy farming in Yogyakarta. Isolation and identification of S. aureus based on the culture, Gram staining and biochemical test. Phenotypes of S. aureus resistances against antibiotics were carried out by disc diffusion method, meanwhilespecies specific gene of S. aureus and the gene encoding methicillin, penicillin and tetracycline were confirmed by PCR method. The results showed 11 isolates representing of Methicillin Susceptible Staphylococcus aureus (MSSA) could be identified, wherein 5 isolates were harboring both of penicillin and tetracycline resistant genes respectively.

Potensi Ekstrak Daun Sage (Salvia officinalis.L) sebagai anti-Streptococcus suis Penyebab Zoonotik Meningitis Mitra Slipranata; Fajar Budi Lestari; Novra Arya Sandi; Siti Isrina Oktavia Salasia
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The phenomenon of microbial resistance to an antibiotic or some kind of specific antibiotics (multi drugs resistance) greatly complicate the treatment process, one of which is Streptococcus suis (S. suis) which is known to cause meningitis in animals and humans. Today, with rising bacterial resistance to a wide rangeof antibiotics, it takes an effort to assess the potential medicinal plants as an antibiotic that is appropriate and safe. Sage (Salvia officinalis.L) is reported to have antibacterial and fungicidal effect. Phenolic acids such assalvin and ether monomethyl salvin which isolated from the sage thought to have antimicrobial activity against several bacterial strains. The purpose of this study was to prove the ability of sage leaf extract as an antibacterialagainst S. suis causes streptococcal meningitis in vitro. In-vitro method used in this research through a two-stage dilution test and the diffusion test on Mueller Hinton Agar (MHA). S. suis isolates (code 225) were tested in vitro against 8 levels sage leaf extract concentration, ie a concentration of 1%, 3% 5%, 7%, 10%, 20%, 40% and 60%. The results of the test obtained by minimum inhibitory concentrations (MICs) and minimum sage known to potentially inhibit the growth of S. suis.

Klebsiella pneumoniae Isolated from Subclinical Mastitis Milk of Etawah Crossbreed Goat Clara Ajeng Artdita; Fajar Budi Lestari; Achmad Fauzi; Erian Pemila Ayu Tanzila
Jurnal Sain Veteriner Vol 36, No 2 (2018): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Klebsiella spp. is a common bacteria causing mastitis. Klebsiella pneumoniae and Klebsiella varicola is the most infected Klebsiella spp in the dairy farm. This study used 16 milk samples from 8 lactation goats in Sahabat Ternak farm, Sleman, Yogyakarta. Samples were tested by california mastitis test (CMT) reagent to determine the mastitis status, inoculated on blood agar (BA) then on Mac Conkey Agar (MCA) as a selective media for Gram negative bacteria. The colonies from BA and MCA were stained by Gram staining to determine Gram-positive or Gram-negative bacteria and its cell morphology. The colonies from MCA were identified by biochemical tests such as sugar fermentation tests (glucose, lactose, maltose, and saccharose), arginine, ornithine decarboxylase, indole, citrate, urea hydrolysis (Christensen’s method), lysin and malonate. The result of mastitis test showed 5 goats were positive result and 3 samples were negative. Bacterial staining showed 2 samples were Gram-negative, rods and the others were Gram-positive, coccus. The samples with rods shapes were continued by biochemical tests. The characterization result of biochemical test indicated that the rods shapes bacteria were Klebsiella pneumoniae. These bacteria identification conducted in Laboratorium Preklinis Program Studi Kesehatan Hewan, Sekolah Vokasi Universitas Gadjah Mada and the result confirmation using positive control of Klebsiella pneumoniae conducted in Balai Laboratorium Kesehatan Yogyakarta. These results showed that milk from Etawah crossbreed goat was infected by Klebsiella pneumoniae.

Potensi Ekstrak Atuna racemosa sebagai Anti - Methicillin Resistant Staphylococcus aureus (MRSA) Siti Isrina Oktavia Salasia; Novra Arya Sandi; Fajar Budi Lestari; Verda Farida; Nurbani Aziz
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Staphylococcus aureus is one of the major causative agents of mastitis in animals and a variety of human diseases such as septicemia, endocarditis, arthritis dan osteomyelitis. Infection of Methicillin-resistant S. aureus (MRSA) has been widely reported and these strains are usually resistant to multiple antibiotics. The purpose of this study was to evaluate the potential of Atuna racemosa, as an alternative herbal medicine against MRSA infection. The MRSA strains were isolated from human and confirmed based on their resistant to various antibiotics and analyzing of the mecA gene by polymerase chain reaction (PCR). Atuna racemosa originated from Ambon, Maluku, Indonesia, were extracted using 70% ethanol. The activities of the Atuna racemosa extract against MRSA were performed by diffusion disc agar and dilussion agar tests. The results showed that Atuna racemosa extract has the barrier effect of MRSA growth at a concentration of 5% in the diffusion test and at a concentration of 7% in the dilution test. Atuna racemosa could be used as an alternative new drugs with dose of 0.07 g/ml (7%) against MRSA which is multi-resistant to many antibiotics.

Deteksi Staphylococcus aureus dan Staphylococcus sp. Secara Langsung Dari Susu Segar Kambing Peranakan Etawa dengan Teknik PCR Fatkhanuddin Aziz; Fajar Budi Lestari; Sarah Nuraida S; Endah Purwati; Siti Isrina Oktavia Salasia
Jurnal Sain Veteriner Vol 38, No 2 (2020): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.53802

Genus Staphylococcus merupakan salah satu patogen bakteri penyebab mastitis yang menyebabkan kerugian ekonomi pada kambing Peranakan Etawa. Diantara Staphylococcus sp. yang dapat tumbuh dengan baik dalam susu segar, diketahui Staphylococcus aureus dapat membahayakan kesehatan manusia yang mengkonsumsi (food borne disease) karena kemampuannya dalam memproduksi enterotoksin yang tahan terhadap enzim pencernaan maupun pemanasan. Tujuan penelitian ini adalah mendeteksi Staphylococcus sp. dan S. aureus secara langsung dari susu kambing peranakan etawa dengan teknik PCR.Metode yang dilakukan adalah mengekstraksi DNA dari 60 sample susu segar dengan prinsip spin column-based nucleic acid purification dan kemudian dilakukan amplifikasi gen spesifik 23S rRNA Staphylococcus sp. dan S. aureus. Hasil PCR diketahui 37 (61%) sampel susu positif mengandung Staphylococcus sp. dan hanya 1 (1,6%) sampel mengandung S. aureus. Metode deteksi dengan PCR dapat digunakan untuk mendeteksi kontaminan Staphylococcus sp. dan S. aureus dengan waktu yang singkat

Pemanfaatan Limbah Buah Salak Pondoh Sebagai Substrat Nata De Salacca Melalui Aplikasi Bioteknologi di Dusun Tegal Domban, Sleman, Yogyakarta Rarastoeti Pratiwi; Fajar Budi Lestari; Donny Widianto
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 1, No 1 (2015): September
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Dusun Tegal Domban is one of the highest Salak Pondoh produce in Sleman District. However there is a problemfaced by salak farmers due to the overproduction and overripe which mightcause waste. Implementation of Biotechnology should be one of the alternativesolutions to overcome this problem. The aims of the program were to utilize the salak fruits wasteto produce Natade Salaccausing biological agents, such as Acetobacter xylinum, and to implement the education for sustainability development which subjected toa women organization PKK Dusun Tegal Domban who accompanyed by the team from Biotechnology Master Program UGM in order to learn how to produce Nata de Salacca. This program was initiated with the laboratory experiments to find the fine composotion of nata substrates to get the optimal nata product. The following action was to acompany the women who aplicate the nata production process. Results from the laboratory experiment showed that the best composition of substrat and water ratiowas 1:4, and the nata thickness was 0.62 cm, while the nata weight was 542.22 g. Those parameters were used for nata standard indicators.Results from the activities of nata production by women groups PKK Dusun Tegal Domban were showed the similar results with the nata standard for thickness, however nata weight was slightly lower than the nata standard.

The Empowerment of Livestock Farmers in Subclinical Mastitis Test with GAMA Anti-Haptoglobin in “Sahabat Ternak” Etawah Crossbreed Goat Farm Fajar Budi Lestari; Madarina Wasissa; Muhammad Novrizal Abdi Sahid; Siti Isrina Oktavia Salasia
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 8, No 1 (2022)
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Sahabat Ternak is one of the Etawah crossbreed (PE) goat farm groups in Sleman. This farm group focuses on goat milk production and processed goods. Problems that commonly arise in dairy goat farms are the cases of subclinical mastitis, which are quite high. This disease may cause a decrease in milk production and quality. The mastitis subclinical detection method which is often used by the farming community is the somatic cell count (SCC) and California mastitis test (CMT). However, both tests have low accuracy. Recently, a new method named GAMA Anti-Haptoglobin, which is more accurate and can be done easily has been established by livestock farmers. This community service aims to empower livestock farmers in applying GAMA Anti-Haptoglobin as a sensitive, rapid, and accurate subclinical mastitis detection kit in Sahabat Ternak goat farm. The method used in this activity consisted of discussion, socialization, and training for livestock farmers, as well as laboratory testing, evaluation of test findings, and treatment for PE goats. After the training, the livestock farmers were able to apply GAMA Anti-Haptoglobin mastitis detection method effectively. The implementation of this easy and accurate field mastitis detection method, as well as personnel with reliable skills, will support in the decrease of mastitis cases and increase milk production and quality, as well as community welfare.

PENGARUH KARAKTERISTIK PETERNAK TERHADAP ADOPSI TEKNOLOGI PEMELIHARAAN PADA PETERNAK KAMBING PERANAKAN ETTAWA DI DESA HARGOTIRTO KABUPATEN KULON PROGO NURULIA HIDAYAH; CLARA AJENG ARTDITA; FAJAR BUDI LESTARI
Jurnal Bisnis dan Manajemen (Journal of Business and Management) Vol 19, No 1 (2019)
Publisher : Magister Manajemen Fakultas Ekonomi dan Bisnis UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbm.v19i1.30916

Livestock in Indonesia are mostly smallholder farms which have the following characteristics, including small scale businesses and using simple technology. One effort to overcome these problems is to carry out technological transformation. The introduction of technology for the maintenance of Peranakan Ettawa (PE) goats has been given to farmers, but not many of these technologies have been adopted by farmers who have certain characteristics. This study aims to analyze the characteristics of farmers who influence the technology adoption rates of Ettawa Peranakan goat breeding. The materials used were Peranakan Ettawa breeders who were members of the Mantep Makaryo, Subur and Tirto livestock groups in the village of Hargotirto, Kokap District, Kulon Progo Regency. The method used in this study is the survey method. The study was conducted from April to October 2018. The analysis used in this study is multiple linear regression analysis, namely the analysis used to determine the factors that influence the rate of technology adoption. The total respondents used in this study were 74 breeders. The results of the analysis showed that at the same time the characteristics of breeders, age, education level, number of family dependents, experience of breeding, number of lactation PE goats, and scale of ownership of PE goats had an influence on 67.20% goat maintenance technology adoption. Partially showing a significant effect (P <0.01) is the experience of farming, education level (P <0.05) while the number of dependents shows the effect on technology adoption (P <0.1). Keywords: Adoption, Characteristics of breeders, PE Goats

Co-Authors Achmad Fauzi Alhadz, Ghias Ghifari Anggraini, Mila Putri Budiyanto, Yohanes Wawan Christin Marganingsih Santosa Clara Ajeng Artdita Clara Ajeng Artdita Clara Ajeng Artdita, Clara Ajeng Donny Widianto Endah Purwati Endah Purwati Erian Pemila Ayu Tanzila Fatkhanuddin Aziz, Fatkhanuddin Hidayah, Nurulia Hidayatullah, Muhammad Fatan Kustika, Estha Gusmalia Madarina Wasissa Maharani, Ananda Peurine Syafira Maulana, Husni Mitra Slipranata Muhammad Novrizal Abdi Sahid Novra Arya Sandi Novra Arya Sandi Nurbani Aziz NURULIA HIDAYAH Rahmayanti, Desyah Rarastoeti Pratiwi Rini Widayanti Sarah Nuraida S Sarah Nuraidah Siti Isrina Oktavia Salasia Siti Isrina Oktavia Salasia Siti Isrina Oktavia Salasia Siti Isrina Oktavia Salasia Wasissa, Madarina Yenni, Latifah Yosyana, Alyaa Rifqoh Putri Yuliawati, Priskilla Sandi

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