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All Journal Jurnal Sain Veteriner Jurnal Veteriner Jurnal Pertanian Agros Prosiding Seminar Nasional Program Pengabdian Masyarakat
Wahyu Prihtiyantoro
Akademi Peternakan Brahmaputra, Yogyakarta
Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Economics, Econometrics & Finance Medicine & Pharmacology Social Sciences Veterinary

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Karakterisasi Faktor-faktor Virulensi Staphylococcus aureus Asal Susu Kambing Peranakan Ettawa secara Fenotip dan Genotip Khusnan Khusnan; Wahyu Prihtiyantoro; Hartatik Hartatik; Mitra Slipranata
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (567.206 KB) | DOI: 10.22146/jsv.22825

Abstract

Staphylococcus aureus is a major cause of mastitis in large or small ruminants, and often manifested by subclinical mastitis in Peranakan Ettawa (PE) goats. Staphylococcus aureus in human can cause food borne disease. The research aimed to characterize the virulence factors of Staphylococcus aureus isolated from milk PE goats, phenotypic- and genotypically. Phenotypically characterization were determined through the pigmen assay as well as hydrophobicity, haemolysin, and hemaglutinin reaction. Polymerase chain reaction (PCR) analysis was used to detect 4 virulen genes including coa, clf, fnbA, and fnbB genes. The results of research showed that Staphylococcus aureus abled to produce white pigmen (35,7%), yellow pigmen g (57,1%), andorange pigmen (7,2%). Staphylococcus aureus showed α-hemolysis zone (35,7%), β-hemolysis (35,7%), dan γ-hemolysis (28,9%). Hydrophobicytic test revealed 14,3% Staphylococcus aureus isolates were hydrophobe and85,7% hydrophil. Staphylococcus aureus (85,7%) isolates abled to aglutinated sheep blood cells. Based on genotypic analysis of Staphylococcus aureus could be detected coa gene (92,8%), clf gene (64,3%), fnbA gene (78,6%), and fnbB gene (64,3%). Based on the phenotypic and genotypic characters, it can be concluded that Staphylococcus aureus are virulent strains. This information can be used as the basis for control mastitis in PE goats
Prevalensi Strain Avian Pathogenic Escherichia coli (APEC) Penyebab Kolibasilosis pada Burung Puyuh Wahyu Prihtiyantoro; Khusnan Khusnan; Mitra Slipranata; Imron Rosyidi
Jurnal Sain Veteriner Vol 37, No 1 (2019): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (902.827 KB) | DOI: 10.22146/jsv.48520

Abstract

Avian Pathogenic Escherichia coli (APEC) is a pathogen that causes colibacillosis in poultry, including salpingitis, omphalitis, cellulitis, swollen head syndrome, coligranuloma yolk sac inflammation, and air sacs inflammation. APEC is a zoonotic strain which spread through raw meat and processed meat products of animals and birds. In this research, the isolation and identification of Escherichia coli were done by using selective media MacConkey, Kligger Iron Agar, and Gram staining. Polymerase chain reaction (PCR) was used to analyse genetopically to detect 16SrRNA genes, vt1 genes, and vt2 genes. Thirty one (55,36%) isolates of 56 specimens collected from quail were detected as Escherichia coli. The detection of APEC strains towards 31 Escherichia coli isolates were done by using polymerase chain reaction (PCR) with vt1 and vt2 specific primer. The results showed that 32,26% (10/31) was APEC strains and 67.74% was non-APEC strains. From 10 isolates, 90% had vt1 gene and 10% had vt2 gene. Escherichia coli isolates were found in eyes (32,26%), infraorbital sinus fluid (32,26%), nasal fluid (16,20%), also in lungs, air sacs, ascites, and heart for 3,2% each. The isolates could not be found in the specimens from the skull. As a zoonotic agent, the isolates have an impact on human health. 
Peran Hemaglutinin dan Hemolisin pada Escherichia coli Sorbitol-negatif Isolat Burung Puyuh pada Proses Infeksi Secara in Vitro Khusnan Khusnan; Wahyu Prihtiyantoro; Dwi Kusmanto
Jurnal Sain Veteriner Vol 39, No 3 (2021): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.69896

Abstract

Abstract Escherichia coli sorbitol-negative in quails cause economic loss due to the death, growth rate inhibition, decreased egg production, and increased medical treatment. Escherichia coli sorbitol-negative has many virulence factors, including hemagglutinin and hemolysin. The aim of this study is to determine the role of hemagglutinin and hemolysin in the infection process of Escherichia coli sorbitol-negative in vitro. This study was performed using 23 isolates of Escherichia coli sorbitol-negative from quails, 52.2% (12 of 23 isolates) had hemagglutinin, while 34.8% (8 out of 23 isolates) had hemolysin. Isolates with hemagglutinin were more attached to human buccal epithelial cells than isolates without hemagglutinin (P <0.05). Isolates with hemolysin were less phagocyted by macrophages compared to isolates which without hemolysin (P <0.05). Escherichia coli sorbitol-negative isolates from quails that have hemagglutinin and hemolysin are pathogenic isolates that possess the potential to cause colibasilosis and transmission between quails and other birds. 
Staphylococcus aureus Penghasil Pigmen Kuning yang Diisolasi dari Kejadian Bumblefoot pada Broiler Lebih Patogen Dibanding Penghasil Pigmen Putih (STAPHYLOCOCCUS AUREUS PRODUCING YELLOW PIGMENT ISOLATED FROM BUMBLEFOOT CASE IN BROILER CHICKENS IS MORE PAT Khusnan .; Wahyu Prihtiyantoro; Mitra Slipranata
Jurnal Veteriner Vol 15 No 4 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (119.768 KB)

Abstract

A study to evaluate the correlation between the pigments produced by Staphylococcus aureusisolateswith their adhesion capability on epithelial cells and phagocytes activity of macrophages in vitro.Ten S.aureus isolatesfrom bumble foot and arthritis casesin broiler were used in this study. The adhesion assaywas performed using epithelial cell derived from human buccal epithelial cells whereas phagocytic activitywas conducted using mouse peritoneal macrophage cells.The results showed that S. aureus isolates frombroiler produced both yellow and white pigments. Staphylococcus aureus producing white pigment adheredto human buccal epithelial cells with the density of about 24,25 bacteria/cell, which were higher than thoseof Staphylococcus aureus producing yellow pigmentabout 23,78 bacteria/cell. Staphylococcus aureusproducing white pigment was more phagocytosed by macrophages than S. aureusproducing yellow pigment,with an average phagocytic activiry of19,86 bacteria/cellas compared to15,96of bacteria/cell, respectively.
KARAKTERISASI FAKTOR VIRULENSI Escherichia coli PATOGEN ZOONOTIK (O157:H7) ISOLAT ASAL TINJA SAPI POTONG CHARACTERIZATION OF Escherichia coli VIRULENCE FACTORS OF ZOONOTIC PATHOGENS (O157:H7) ISOLATE OF FECAL CATTLE Wahyu Prihtiyantoro; Hartatik Hartatik; Khusnan Khusnan; Mitra Slipranata; Fatkhanudin Aziz
Agros Journal of Agriculture Science Vol 16, No 2: Edisi Juli 2014
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (272.171 KB)

Abstract

Kemampuan hemaglutinasi, keberadan hemolisin dan kekebalan terhadap antibiotik merupakan faktor virulensi yang penting pada Escherichia coli. Penelitian ini bertujuan melakukan karakterisasi faktor virulensi terhadap 9 (sembilan) E. coli isolat sapi potong yang terdiri 5 (lima) isolat berasal dari tinja dan 4 (empat) isolat berasal dari pupuk kandang. Duapuluh dua persen isolat mampu menggumpalkan eritrosit dan 100 persen isolat tidak memiliki hemolisin, 4 isolat asal tinja positif sebagai patogen zoonotik (O157:H7). Kekebalan isolat oleh eritromisin, metisilin, penisilin, tetrasiklin dan gentamisin  masing-masing 66,7 persen, 66,7 persen, 66,7 persen, 16,7 persen dan 0 persen. Resistensi hanya terjadi pada isolat asal tinja sedangkan isolat asal pupuk kandang masih sensitive terhadap antibiotika-antibiotika tersebut. Hemagglutination ability, the existence of hemolysin and antibiotic resistance is an important virulence factor in Escherichia coli. This study aimed to characterize the virulence factors of the nine (9) E. coli isolates beef cattle comprising five (5) isolates from feces and 4 (four) isolates from manure. Twenty-two percent of isolates were able to aglutinate erythrocytes and 100 percent of isolates did not have hemolysin. Immunity isolates by erythromycin, methicillin, penicillin, tetracycline and gentamicin respectively 66.7 percent, 66.7 percent, 66.7 percent, 16.7 percent and 0 percent. Resistance occurs only at the fecal isolates while isolate of manure is still sensitive to the antibiotics
KEMAMPUAN ADHESI Escherichia coli HEMAGLUTINASI DAN SMAC POSITIF ISOLAT SAPI POTONG PADA SEL EPITEL BUKALIS SECARA IN VITRO Wahyu Prihtiyantoro; Hartatik Hartatik; Khusnan Khusnan
Agros Journal of Agriculture Science Vol 18, No 2: Edisi Juli 2016
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (316.338 KB)

Abstract

Adhesi bakteri pada permukaan sel epitel inang merupakan awal dari proses infeksi. Bakteri yang mempunyai faktor-faktor virulensi  dan faktor adhesin lebih mudah beradhesi pada sel epitel. Penelitian ini bertujuan untuk mengetahui kemampuan adhesi isolat-isolat Escherichia coli yang memiliki hemaglutinin dan strain Escherichia coli zoonotik pathogenic (VTEC). Isolat-isolat yang memiliki hemaglutinin dan  strain VTEC memiliki kemampuan adhesi yang lebih tinggi dibandingkan dengan isolat-isolat yang tidak memiliki hemaglutinin dan strain non VTEC.
Penyuluhan Manajemen Kambing Perah di KPP Pangestu Desa Kemirikebo Ferriawan Yudhanto; Rinasa Agistya Anugrah; Venditias Yudha; Wahyu Prihtiyantoro; Citravia Agustin
Prosiding Seminar Nasional Program Pengabdian Masyarakat 2022: 5. Produktifitas dan Daya Saing Industri Pangan
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/ppm.55.1063

Abstract

Keberhasilan suatu usaha peternakan dapat dicapai melalui bibit, pakan dan manajemen. Rendahnya produktivitas kambing perah di KPP. Pangestu menjadi masalah utama usaha peternakan tersebut. Peternakan KPP. Pangestu memiliki ternak kambing sebanyak 1300 kambing perah. Tujuan dari pengabdian kolaboratif-UMY ini adalah memberikan pengetahuan kepada peternak dalam manajemen kambing perah yang baik sehingga produksi susu dapat meningkat. Penyuluhan merupakan salah satu metode untuk memberikan wawasan pengetahuan bagi peternak tentang pentingnya penambahan nutrisi pada pakan ternak ruminansia. Hasil dari kegiatan ini adanya peningkatan produksi susu dari 300 menjadi 450 lt/hari
Co-Authors Citravia Agustin Dwi Kusmanto Fatkhanudin Aziz Ferriawan Yudhanto Hartatik Hartatik Hartatik Hartatik Hartatik Hartatik Imron Rosyidi Khusnan k Mitra Slipranata Mitra Slipranata Mitra Slipranata Mitra Slipranata Mitra Slipranata Novra A. Sandi Sudarisman Sudarisman Yudha, Venditias