Woro Danur Wendo
Department Of Anatomy, Faculty Of Veterinari Medicine Universitas Gadjah Mada

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Distribusi neuron serotonergik pada hipokampus Lasiwen (Myotis sp) sebagai satwa liar yang berpotensi menjadi reservoir virus rabies Tri Wahyu Pangestiningsih; Ariana Ariana; Irma Padeta; Arvendi Rahma Jadi; Woro Danur Wendo
Jurnal Sain Veteriner Vol 37, No 1 (2019): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (7264.05 KB) | DOI: 10.22146/jsv.42914

Abstract

Myotis sp is an Indonesian microchiropteran, insectivore bat that potential to be a reservoir for rabies virus. Rabies is fatal viral encephalitis and  bat could  act as wildlife potential reservoirs for the rabies virus and can transmit the disease to humans as the zoonotic disease. Hippocampus  is one of diagnostic tissue for rabies disease and serotonergic neuron could involved in pathogenesis of rabies disease.  The aim of the study is to investigate the distribution of serotonergic neurons in  Myotis sp hippocampus. Five Myotis sp. were captured from wild population in Central Java, and were humanly anesthetized using ketamine and xylazin. The animals were perfused intracardially using NaCl 0.9% as the pre-rinse followed by 10% formaldehyde to fix it. The cerebrums were  collected and processing the  for paraffin embedding. Cerebrums were sectioning in saggital sections,  12 µm thickness serially with 120 µm intervals.    The tissues  were staining immunohistochemistry using antibody to serotonin (1/300; Bioss, Cat. No: bs-1126R) 2 night incubation in 4 oC temperature. The solution for blocking background, secondary antibody, avidin-biotin-peroxidae complex and chromogen using  kit Starr Trek Universal HRP Detection System (Biocare Medical, Cat No: STUHRP700) and were analyzed descriptivelly. The results show that serotonergic neuros were distributed in the all area of  the of Myotis sp hippocampus. In  dentate gyrus neuron serotonergic (Sert-IR)s are round in shape and  mostly distributed in the middle layer, few in the  superficially also deeper layers. In the hippocampus , the Sert-IR neurons are pyramidal in shape and distributed in the CA1, Ca2 and CA3 areas. In subiculum, the Sert-IR neurons  are pyramidal in shape, more wider distributed than in the CA1 with no differences between outer layer and deeper layer.  The conclutions of this research are the serotonergic neurons are distributed in the all area of hippocampus
The Anatomical Study of Trachea in The Common Palm Civet (Paradoxurus hermaphroditus) Martina Marina; Audrey Tabitha Gracia; Arvendi Rachma Jadi; Woro Danur Wendo
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 5 N0. 2, JULY 2021
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v5i2.12054

Abstract

The common palm civet (Paradoxurus hermaphroditus) is one of the wild animals that can be found across Java, Sumatra, Kalimantan, Sabah, Sarawak, India, and Sri Lanka. Civet is considered as a nocturnal and arboreal animal. This study aims to determine the anatomical morphology of the civet’s trachea, which information is still limited. The trachea of three adult civets of different sexes obtained from Yogyakarta and Lampung were used in this study. Samples were collected, diffused, examined macroscopically, and microscopically processed to produce paraffin blocks. Paraffin blocks were sliced of thickness 4µm using a microtome. Samples were stained with hematoxylin-eosin. The staining results were then described to determine the tracheal profile of the civet’s trachea. Histologically, trachea has several layers successively from the inside out are the mucosa, submucosa, hyaline cartilage layer, and tunica adventitia. The mucosal layer consists of the epithelium, lamina propria, and the invisible muscularis mucosa. The epithelium portion of the trachea is a ciliated stratified pseudo columnar containing goblet and basal cells. Column cells are oval with a dark cell nucleus at the basal area. The lamina propria part of the civet’s trachea consists of connective tissue consisting of collagen, elastin, and reticular fibers. There are seromucous glands between the lamina propria and submucosa. The tracheal musculus is located on the external side of the hyaline cartilage. Keywords: Common Palm Civet, Hematoxylin Eosin, Histology, Morphology, Trachea
Mandibular and Teeth Osteomorphology in Common Palm Civet (Paradoxurus hermaphroditus) Arvendi Rachma Jadi; Woro Danur Wendo; Hery Wijayanto
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 6 No 2, JULY 2022
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v6i2.22351

Abstract

Common palm civet has another name “musang luwak” and is included in the Viverridae family. A civet is a small mammal that lives at night (nocturnal) and likes to eat fruit (frugivorous). The population of this animal is spread across Indonesia, Sri Lanka, India, and some South East Asia countries. Osteological studies on civets have not been done much. This study aims to determine the anatomical structure of the mandible and teeth macroscopically. This study used 3 civet craniums with an average body weight of around 2 kg obtained from Yogyakarta and Lampung. The samples separated from the muscles and tissues to obtain the cranium, mandible, and teeth. The cleaned samples were then put in 5.25% Sodium Hypochlorite for 24 hours to make the measurement easier. The next process is rinsing with running water and drying. Morphological studies were carried out by examining the teeth of the civet and comparing them with carnivores in the literature. Morphological craniomandibular data were observed specifically for the existing formations. In the mandible, there is a characteristic formation at the angle of the mandible which is tapered and the teeth are pyramidal in shape with the carnassial part which is a pair of pointed upper and lower teeth used for cutting food. The last two molars have a more sloping shape because they are closely related to the function of crushing bones. The dental formula in civets was formulated by incisivus (3), caninus (1), premolars (4), and molars (2) with a total of about 40 permanent teeth. Keywords: Common Palm Civet, Craniomandibular; Gross anatomy; Osteo morphology, Teeth
Small intestine histologic neuronal features of type-2 diabetes mellitus rats treated with ethanolic extract red betel leaf (Piper crocatum) nanoparticle (EERbLNp) Tri Wahyu Pangestiningsih; Roza Azizah Primatika; Teguh Budipitojo; Woro Danur Wendo
Livestock and Animal Research Vol 21, No 1 (2023): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/lar.v21i1.67929

Abstract

Objective: This study marked histological features of small intestine neurons in the type-2 diabetes mellitus (T2DM) rat model treated with ethanolic extract red betel leaf nanoparticle (EERbLNp)Methods: Thirty male Wistar rats were allotted into five groups (six rats each). Group I is nondiabetic control; group II is streptozotocin-nicotinamide (STZ-NA)-induced T2DM; group III-V are the STZ-NA-induced T2DM treated daily per oral with EERbL-Np at the doses 30, 60, and 90 mg/kg, respectively, within 28days. The duodenum, jejunum, and ileum were collected for routine histological staining and cresyl violet special staining to evaluate the feature of neurons in the Meissner (submucosa) and Auerbach (muscular) plexus. Descriptive statistical analysis ANOVA and Tukey HSD were used to compare neuron indexes among groups.Results: The necrotic neuron index in the duodenal and ileal Auerbach plexus, including the degenerative neuron index in the jejunal Auerbach plexus, were significantly decreased with the EERbL-Np treatment at the dosages 60 and 90mg/kg.Conclusions: The 60 mg/kg EERbL-Np administration in the T2DM model may provide a neuroprotectant candidate in duodenal and ileal neuropathy. Dosage of 90mg/kg EERbL-Np is also promising in jejunal neuropathy treatment.