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Studi Distribusi Glukosa Transporter 4 pada Otot Skelet Ayam Kedu Cemani dengan Metode Imunohistokimia Avidin-Biotin-Peroxidase Complex Teguh Budipitojo; Ariana Ariana; Tri Wahyu Pangestiningsih; Hery Wijayanto; Dwi Liliek Kusindarta; Dewi Kania Musana
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Glucose Transporter 4 (GLUT 4) merupakan protein transporter glukosa yang diatur oleh insulin, ditemukan terutama di jaringan adiposa dan otot lurik (baik otot skelet maupun otot jantung). Ayam kedu cemani merupakan ayam endemik Indonesia yang terdapat di wilayah Kedu, Temanggung, Jawa Tengah. Penelitian ini diperlukan untuk melengkapi dokumentasi data anatomi mikroskopik kekayaan hayati asli Indonesia. Penelitian ini bertujuan untuk mengklarifikasi distribusi GLUT 4 dalam serabut otot skelet ayam kedu dengan metode imunohistokimia avidin-biotin-peroxidase complex (ABC). Penelitian dilakukan dengan menggunakan jaringan otot yang meliputi otot pectorales mayor, biceps brachii, dan biceps femoris dari 5 ekor ayam kedu cemani. Hasil penelitian menunjukkan bahwa immunoreaktivitas GLUT 4 terutama terdeteksi di sarkolema dan komponen miofibril pada otot pectorales mayor, biceps brachii, maupun biceps femoris. Intensitas imunorekatifitasnya meningkat dari intensitas lemah pada otot pectorales mayor, menjadi intensitas sedang pada otot biceps brachii, dan kemudian intensitas kuat pada otot biceps femoris. Hasil penelitian memunculkan dorongan untuk menggali lebih lanjut ciri-ciri khusus ayam kedu cemani lainnya, untuk mendokumentasi data anatomis kekayaan hayati asli Indonesia.

PENDAMPINGAN PEMBELAJARAN BIOLOGI ANATOMI HEWAN SECARA DARING UNTUK SEKOLAH-SEKOLAH DASAR GUGUS VI DI DESA TUKSONO, SENTOLO, KULON PROGO, YOGYAKARTA : Online Animal Anatomy Biology Learning Assistance for Cluster VI Elementary Schools in Tuksono Village, Sentolo, Kulon Progo, Yogyakarta Arvendi Rachma Jadi; Hery Wijayanto; Dwi Liliek Kusindarta; Vista Budiariati; Tri Wahyu Pangestiningsih; Teguh Budipitojo; Ariana; Hevi Wihadmadyatami
IGKOJEI: Jurnal Pengabdian Masyarakat Vol. 3 No. 2 (2022): IGKOJEI: Jurnal Pengabdian Masyarakat
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/igkojei.v3i2.301

ABSTRACT The problem faced during the new normal period of the Covid-19 pandemic for students is to restore interest in learning that has been done at home. Additional efforts are needed to reduce these concerns, such as mentoring for learning outside of school. Community service in the form of learning assistance is carried out with the aim of providing new experiences, information, and knowledge outside of learning at school. The schools participating in the service activities are schools from Cluster VI Tuksono Village, Sentolo, Kulon Progo, Yogyakarta. This activity involves student assistants from the Anatomy Department, Faculty of Veterinary Medicine, Universitas Gadjah Mada with assistance for a certain period. Implementation of online activities providing materials and discussions that have been adapted to the competencies needed in schools. The material given is an introduction to vertebrates macroscopically and microscopically. Students will be divided into several classes and each has an assistant and a co-lecturer. The activity takes place from September to November 2021. The results of the questionnaire show that this learning assistance activity is very interesting (60%) and very useful (69%) for students and teachers in their schools. Keywords: Animal, Biology Anatomy; Elementary school; Learning; Online ABSTRAK Permasalahan yang dihadapi pada masa normal baru pandemi Covid-19 bagi peserta didik adalah mengembalikan minat belajar yang selama ini dilakukan di rumah. Usaha untuk mengurangi keprihatinan tersebut diperlukan seperti pendampingan belajar luar sekolah. Pengabdian kepada masyarakat berupa pendampingan pembelajaran dilaksanakan dengan tujuan memberikan pengalaman, informasi, dan pengetahuan baru di luar pembelajaran di sekolah. Sekolah yang berpartisipasi dalam kegiatan pengabdian adalah sekolah-sekolah dari Gugus VI Desa Tuksono, Sentolo, Kulon Progo, Yogyakarta. Kegiatan ini melibatkan asisten mahasiswa Departemen Anatomi Fakultas Kedokteran Hewan Universitas Gadjah Mada dengan pendampingan selama periode tertentu. Pelaksanaan kegiatan daring pemberian materi dan diskusi yang sudah disesuaikan dengan kompetensi yang dibutuhkan di sekolah. Materi yang diberikan berupa pengenalan vertebrata secara makroskopik dan mikroskopik. Peserta didik akan dibagi menjadi beberapa kelas dan masing-masing memiliki satu asisten dan dosen pendamping. Kegiatan berlangsung dari bulan September sampai November 2021. Hasil kuisioner menunjukkan kegiatan pendampingan pembelajaran ini sangat menarik (60%) dan sangat bermanfaat (69%) bagi para peserta didik dan guru di sekolahnya. Kata kunci: Anatomi Biologi; Daring; Hewan; Pembelajaran; Sekolah Dasar

Mandibular and Teeth Osteomorphology in Common Palm Civet (Paradoxurus hermaphroditus) Arvendi Rachma Jadi; Woro Danur Wendo; Hery Wijayanto
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 6 No 2, JULY 2022
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v6i2.22351

Common palm civet has another name “musang luwak” and is included in the Viverridae family. A civet is a small mammal that lives at night (nocturnal) and likes to eat fruit (frugivorous). The population of this animal is spread across Indonesia, Sri Lanka, India, and some South East Asia countries. Osteological studies on civets have not been done much. This study aims to determine the anatomical structure of the mandible and teeth macroscopically. This study used 3 civet craniums with an average body weight of around 2 kg obtained from Yogyakarta and Lampung. The samples separated from the muscles and tissues to obtain the cranium, mandible, and teeth. The cleaned samples were then put in 5.25% Sodium Hypochlorite for 24 hours to make the measurement easier. The next process is rinsing with running water and drying. Morphological studies were carried out by examining the teeth of the civet and comparing them with carnivores in the literature. Morphological craniomandibular data were observed specifically for the existing formations. In the mandible, there is a characteristic formation at the angle of the mandible which is tapered and the teeth are pyramidal in shape with the carnassial part which is a pair of pointed upper and lower teeth used for cutting food. The last two molars have a more sloping shape because they are closely related to the function of crushing bones. The dental formula in civets was formulated by incisivus (3), caninus (1), premolars (4), and molars (2) with a total of about 40 permanent teeth. Keywords: Common Palm Civet, Craniomandibular; Gross anatomy; Osteo morphology, Teeth

EVALUATION OF LAPAROTOMY SURGERY WOUND HEALING IN DOGS USING THERMOGRAPHIC ANALYSIS Agustina Dwi Wijayanti; Agus Purnomo; Hery Wijayanto
Jurnal Kedokteran Hewan Vol 17, No 1 (2023): March
Publisher : Universitas Syiah Kuala

This study aimed to develop the use of thermographic analysis to evaluate wound healing for laparotomy surgery in animals. The experiment used nine adult dogs 5 males and 4 females, bodyweight 5-10 kg) that had undergone the same general anesthesia and laparotomy procedures. Evaluation of wound healing was performed at 24, 48, and 72 hours after surgery and 7 days after operations using a digital thermal camera. During each thermographic evaluation period, blood samples were taken for analysis of total leukocytes and leukocyte differential. The results of thermal imaging were compared with the values of inflammatory cells and the clinical condition of wound healing. Comparison of thermographic analysis with inflammatory status was evaluated using the regression equation and showed a strong correlation coefficient (Y= -0.4847 + 40.14, R2= 0.867), that is, the higher the temperature, the lower the inflammation. The conclusion of study is that thermographic analysis can be used to evaluate wound healing of laparotomy surgery in dogs.

The Effect of Caffeine Treatment during Organogenesis Period on the Birth Weight of the Rat Fetuses (Rattus norvegicus) Hery Wijayanto; Tri Wahyu Pangestiningsih; Erdiansyah Rahmi
Jurnal Kedokteran Hewan Vol 1, No 2 (2007): September
Publisher : Universitas Syiah Kuala

The study was conducted to investigate the effects of caffeine treatment during organogenesis period to the fetal birth weight, using rat (Rattus norvegicus) as the animal model. Thirty-six primipararat obtained from Unit Pengembangan Hewan Percobaan, Gadjah Mada University (UPHP-GMU), 3 month old, 165-200 g body weight, were divided into 6 groups, consisted of 6 rats each. Six of the ratshave been selected based on the estrous cycles, and only rat with regular estrous were use for theexperiment. The rat then were mated, and during day 6-14 of the pregnancies were treated orally withcaffeine diluted in aquadest in dosage: placebo (1 cc aquadest) for group I (control), and 5.4, 10.8, 16.2,21.6, and 27 g/200 g body weight/day for treatment groups II-VI respectively. The pregnant rat bodyweights were determined at day 6 of pregnancies for calculating the caffeine treatment dosages. At day 20thof the pregnancies all of the pregnant rats were caesarotomized, and all of the fetuses were removed and weighed. The results showed that all of the treatment groups have significantly lower birth weightcompare to the groups control group. More over, fetal obtained from the treatment groups also showedserious subcutaneous hemorrhagic.Keywords: organogenesis, Rattus norvegicus, birth weight

KAJIAN DIVERSITI GENETIKA Tarsius sp. ASAL INDONESIA MENURUT URUTAN GEN NADH DEHIDROGENASE SUBUNIT 4 (ND4) Herrialfian H; Rini Widayanti; Hery Wijayanto; Jalaluddin J
Jurnal Kedokteran Hewan Vol 8, No 1 (2014): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v8i1.1247

Penelitian ini bertujuan mengkaji keragaman genetik gen penyandi ND4 pada Tarsius bancanus, T. b. borneanus, T. dianae dan T. spectrum dan untuk penegakan taksonominya. Deoxyribonucleic acid (DNA) diisolasi dari biopsi jaringan masing-masing spesies Tarsius dengan cara diekstraksi untuk digunakan sebagai DNA cetakan dalam proses amplifikasi dengan metode polymerase chain reaction (PCR). Primer yang digunakan dalam penelitian ini didesain untuk mengamplifikasi gen ND4 dan dilanjutkan dengan elektroforesis. Produk PCR hasil amplifikasi yang telah dimurnikan, selanjutnya dipergunakan sebagai DNA cetakan untuk reaksi penentuan runutan nukleotida. Runutan nukleotida gen ND4 hasil pengurutan dilakukan penjajaran berganda dengan primata lain yang diambil dari Genbank menggunakan Clustal W. Selain berdasarkan runutan nukleotida, gen ND4 dianalisis berdasarkan runutan asam amino dari basa-basa yang diterjemahkan mengikuti vertebrate mitochondrial translation code yang ada pada program MEGA versi 4.1. Konstruksi pohon filogenetika menggunakan metode neighbor joining. Hasil penelitian menunjukkan dari 1378 nukleotida ditemukan 119 situs yang bersifat beragam. Jarak genetika berdasarkan nukleotida gen ND4 yang dihitung menggunakan model dua parameter Kimura, terdapat nilai paling kecil 0,6%, nilai terbesar 13%, dan nilai rata-rata sebesar 6,1%. Filogram berdasarkan hasil runutan nukleotida gen ND4 yang menggunakan metode neighbor joining, dapat mengidentifikasi dan membedakan percabangan antar spesies Tarsius.

Morphological Structure of the Tongue of Gekko gecko in Yogyakarta, Indonesia Aqiilah, Khairunnisa; Budipitojo, Teguh; Wijayanto, Hery; Budiariati, Vista; Pangestiningsih, Tri Wahyu; Ariana, Ariana; Saragih, Golda Rani; Kustiati, Ulayatul; Wihadmadyatami, Hevi
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 4 (2024): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.90995

Gekko gecko is a member of the order Squamata from the family Gekkonidae and mainly feeds on small insects. This study aims to determine the morphology of the tongue of Gekko gecko through Scanning Electron Microscopy (SEM), hematoxylin–eosin (HE) staining, and immunohistochemistry (IHC). Six adult Gekko gecko were obtained from the Special Region of Yogyakarta, and Gekko gecko tongue samples were stored in SEM fixative solution and then observed with SEM. For histochemical and IHC staining, tongue samples were processed into paraffin blocks and cut into 8 µm-thick sections. The SEM revealed three types of papillae: dome-shaped papillae at the apex, fan-shaped papillae at the corpus, and scale–like papillae at the radix. Histological observations showed that the tongue of the Gekko gecko was composed of tunica mucosa and tunica muscularis, and goblet cells were present in the lamina of the epithelial mucosa. Meanwhile, no taste buds were found. Immunoreactivity against PGP 9.5 was observed on the tunica muscularis of the apex, corpus, and radix. Taken together, this study provides new insight into the tongue morphology of Gekko gecko and is dominated by mechanical papillae on the tongue surface.

EVALUATION OF LAPAROTOMY SURGERY WOUND HEALING IN DOGS USING THERMOGRAPHIC ANALYSIS Wijayanti, Agustina Dwi; Purnomo, Agus; Wijayanto, Hery
Jurnal Kedokteran Hewan Vol 17, No 1 (2023): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v17i1.29785

This study aimed to develop the use of thermographic analysis to evaluate wound healing for laparotomy surgery in animals. The experiment used nine adult dogs 5 males and 4 females, bodyweight 5-10 kg) that had undergone the same general anesthesia and laparotomy procedures. Evaluation of wound healing was performed at 24, 48, and 72 hours after surgery and 7 days after operations using a digital thermal camera. During each thermographic evaluation period, blood samples were taken for analysis of total leukocytes and leukocyte differential. The results of thermal imaging were compared with the values of inflammatory cells and the clinical condition of wound healing. Comparison of thermographic analysis with inflammatory status was evaluated using the regression equation and showed a strong correlation coefficient (Y= -0.4847 + 40.14, R2= 0.867), that is, the higher the temperature, the lower the inflammation. The conclusion of study is that thermographic analysis can be used to evaluate wound healing of laparotomy surgery in dogs.

The Effect of Caffeine Treatment during Organogenesis Period on the Birth Weight of the Rat Fetuses (Rattus norvegicus) Wijayanto, Hery; Pangestiningsih, Tri Wahyu; Rahmi, Erdiansyah
Jurnal Kedokteran Hewan Vol 1, No 2 (2007): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v1i2.3123

The study was conducted to investigate the effects of caffeine treatment during organogenesis period to the fetal birth weight, using rat (Rattus norvegicus) as the animal model. Thirty-six primipararat obtained from Unit Pengembangan Hewan Percobaan, Gadjah Mada University (UPHP-GMU), 3 month old, 165-200 g body weight, were divided into 6 groups, consisted of 6 rats each. Six of the ratshave been selected based on the estrous cycles, and only rat with regular estrous were use for theexperiment. The rat then were mated, and during day 6-14 of the pregnancies were treated orally withcaffeine diluted in aquadest in dosage: placebo (1 cc aquadest) for group I (control), and 5.4, 10.8, 16.2,21.6, and 27 g/200 g body weight/day for treatment groups II-VI respectively. The pregnant rat bodyweights were determined at day 6 of pregnancies for calculating the caffeine treatment dosages. At day 20thof the pregnancies all of the pregnant rats were caesarotomized, and all of the fetuses were removed and weighed. The results showed that all of the treatment groups have significantly lower birth weightcompare to the groups control group. More over, fetal obtained from the treatment groups also showedserious subcutaneous hemorrhagic.Keywords: organogenesis, Rattus norvegicus, birth weight

KAJIAN DIVERSITI GENETIKA Tarsius sp. ASAL INDONESIA MENURUT URUTAN GEN NADH DEHIDROGENASE SUBUNIT 4 (ND4) H, Herrialfian; Widayanti, Rini; Wijayanto, Hery; J, Jalaluddin
Jurnal Kedokteran Hewan Vol 8, No 1 (2014): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v8i1.1268

Penelitian ini bertujuan mengkaji keragaman genetik gen penyandi ND4 pada Tarsius bancanus, T. b. borneanus, T. dianae dan T. spectrum dan untuk penegakan taksonominya. Deoxyribonucleic acid (DNA) diisolasi dari biopsi jaringan masing-masing spesies Tarsius dengan cara diekstraksi untuk digunakan sebagai DNA cetakan dalam proses amplifikasi dengan metode polymerase chain reaction (PCR). Primer yang digunakan dalam penelitian ini didesain untuk mengamplifikasi gen ND4 dan dilanjutkan dengan elektroforesis. Produk PCR hasil amplifikasi yang telah dimurnikan, selanjutnya dipergunakan sebagai DNA cetakan untuk reaksi penentuan runutan nukleotida. Runutan nukleotida gen ND4 hasil pengurutan dilakukan penjajaran berganda dengan primata lain yang diambil dari Genbank menggunakan Clustal W. Selain berdasarkan runutan nukleotida, gen ND4 dianalisis berdasarkan runutan asam amino dari basa-basa yang diterjemahkan mengikuti vertebrate mitochondrial translation code yang ada pada program MEGA versi 4.1. Konstruksi pohon filogenetika menggunakan metode neighbor joining. Hasil penelitian menunjukkan dari 1378 nukleotida ditemukan 119 situs yang bersifat beragam. Jarak genetika berdasarkan nukleotida gen ND4 yang dihitung menggunakan model dua parameter Kimura, terdapat nilai paling kecil 0,6%, nilai terbesar 13%, dan nilai rata-rata sebesar 6,1%. Filogram berdasarkan hasil runutan nukleotida gen ND4 yang menggunakan metode neighbor joining, dapat mengidentifikasi dan membedakan percabangan antar spesies Tarsius.

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