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All Journal Jurnal Sain Veteriner JURNAL PENGABDIAN KEPADA MASYARAKAT JIKAP PGSD: Jurnal Ilmiah Ilmu Kependidikan Jurnal Agrohut Bioscientist : Jurnal Ilmiah Biologi
Sriyati Sampulawa
Universitas Darussalam Ambon
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Education Engineering Immunology & microbiology Languange, Linguistic, Communication & Media Social Sciences Veterinary

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POTENSI ANTIBAKTERI EKSTRAK ALGA HIJAU Halimeda makroloba Decaisne DARI PERAIRAN DESA HUTUMURI KOTA AMBON Sriyati Sampulawa; Wa Nirmala
Jurnal Sain Veteriner Vol 39, No 2 (2021): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.59980

Abstract

Perkembangan penggunaan obat-obatan tradisional  untuk membantu meningkatkan kesehatan masyarakat sudah cukup meluas, salah satu tumbuhan yang sering digunakan dalam bidang kesehatan yaitu alga hijau, penggunaan alga hijau karena memiliki senyawa metabolit sekunder yang efektif dalam menghambat pertumbuhan bakteri, namun isolasi senyawa aktif dari alga hijau yang efektif  dalam menghambat pertumbuhan bakteri masih jarang dilakukan, salah satunya Halimeda makroloba Decaisne sehingga perlu dilakukuan isolasi dan identifikasi senyawa yang aktif dalam menghambat pertumbuhan bakteri. Untuk mengetahui potensi antibakteri ekstrak alga hijau Halimeda makroloba Decaisne dari perairan Desa Hutumuri Kota Ambon dapat dilakukan melalui beberapa tahapan yaitu proses pengambilan sampel, proses ekstraksi, proses uji fitokimia uji daya hambat bakteri. Berdasarkan hasil penelitian uji fitokimia menunjukan bahwa ekstrak alga hijau Halimeda makroloba Decaisne kandungan senyawa Alkoloid, Flavonoid, Terpenoid, Fenolik, dan saponin yang berperan dalam menghambat pertumbuhan bakteri Escherichia coli dan Staphylococcus aureus. Kemampuan ekstrak alga hijau Halimeda makroloba Decaisne dalam menghambat pertumbuhan bakteri Escherichia coli pada konsentrasi 10%, 20%, 40%, 60% dan 80%, dan Kemampuan ekstrak dalam menghambat bakteri Staphylococcus aureus pada konsentrasi 60% dan 80%.
Identifikasi Senyawa Bioaktif Ekstrak Alga Coklat (Hormophysa triquetra) Sriyati Sampulawa; Farida Bahalwan
Bioscientist : Jurnal Ilmiah Biologi Vol 10, No 1 (2022): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v10i1.4918

Abstract

Hormophysa triquetra is a type of brown algae that is easily found along the coast with sandy and rocky sediments. Based on the results of research, some brown algae are known to be often used because they are a rich source of various secondary metabolites. Hormophysa triquetra is a type of brown algae that needs to be investigated for the content of secondary metabolites so that it can be used widely. The research method used is an experimental method with sampling techniques carried out using free samples, which were carried out in the waters of Hutumuri Village, Ambon City, the samples obtained were then dried by aerating, then the algae samples were extracted by the maceration method, then the results of the Hormophysa extract triquetra carried out phytochemical tests. Phytochemical test of Hormophysa triquetra extract included examination of the content of alkaloids, flavonoids, tannins, terpenoids/steroids and saponins. The test results showed that the Hormophysa triquetra algae extract contained flavonoid, terpenoid, phenolic, saponin and alkaloid compounds.
Pemanfaatan Ekstrak Kunyit (Curcuma domestica Val.) sebagai Pewarna Alami Pembuatan Preparat Awetan Jaringan Tumbuhan dalam Praktikum Biologi Sel Asmi Ode; Sriyati Sampulawa; Fauzia Hulopi
Bioscientist : Jurnal Ilmiah Biologi Vol 10, No 2 (2022): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v10i2.5979

Abstract

Making preparations for observing cells or tissues in practicum activities requires dyes. In cell biology practicum, especially observation of plant tissue in the Department of Biology Education, FKIP, Ambon University of Darussalam, it is still very limited, because it does not have chemical dyes for making preserved preparations. This is because chemical dyes are quite expensive, unsafe, and will spoil if stored for a long time, so it is very important to make natural dyes as a substitute for synthetic dyes so that the preserved preparations produced are safer, more affordable, and can be used for a long time. which is old. Sources of natural dyes are obtained from plant parts, for example: roots, bark, leaves, fruit, wood, seeds, and flowers. Turmeric (Curcuma domestica Val.) is a natural dye that can be used, because it is relatively cheap, easy to find, non-carcinogenic, and biodegradable. The active pigments in turmeric which can color plant tissue and give it a yellow color are curcuminoids. The aim of this research was to study the utilization of turmeric extract (Curcuma domestica Val.) as a natural dye for preparing preserved preparations of plant tissues in cell biology practicum. This type of research is experimental research, which has several stages of research including: observation, preparation, research implementation, and analysis stage. At the data analysis stage, a qualitative descriptive method was used to determine the quality of the preserved preparations. The quality of the preparations in this study was expressed by the percentage of eligibility of ≥ 62.5% or feasible criteria. The results showed that the average percentage of the quality of the preparations that had been validated was 66.96% and 67.85% with proper criteria. This shows that the preserved preparations made are suitable for observing plant tissues in cell biology labs.
IMPLEMENTASI MEDIA PEMBELAJARAN LABORATORIUM VIRTUAL DALAM MENDUKUNG PROGRAM MERDEKA BELAJAR PADA SEKOLAH-SEKOLAH DI KECAMATAN SALAHUTU KABUPATEN MALUKU TENGAH Wa Nirmala; Farida Bahalwan; Sriyati Sampulawa; Siti Darmawati; Asmi Ode; Rizky Fatmalasari L; Kasliyanto Kasliyanto; Abdullah Derlean; Pebrywati Watimury
JURNAL PENGABDIAN KEPADA MASYARAKAT Vol 29, No 2 (2023): APRIL-JUNI
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jpkm.v29i2.37694

Abstract

Learning from home (BDR) conditions make students more exposed to gadgets. This phenomenon is a challenge for teachers to further upgrade their competence in the use of technology. Today's learning requires teachers to develop and apply technology in conducting learning to motivate students. The solution that can be offered from partner problems is to increase the ability to use technology for teachers in junior and senior high schools in Salahutu District, Central Maluku Regency. One of the technologies that can be used in science learning in junior high and high school is a virtual laboratory. The method of activity is in the form of training and assistance in making a virtual laboratory which includes several stages, namely: the preparation stage, the team coaching stage, the training and mentoring stage, the closing stage. Based on the results of the satisfaction questionnaires that were distributed to participants during the evaluation, it was found that the average participants of the training and mentoring activities were able to operate macromedia flash software and create simple Virtual Laboratory applications so that Partner Schools still expect further activities to design IT-based learning media that innovative and can be used in online and offline learning.
Pengaruh pemberian larutan tembaga terhadap pertumbuhan tanaman bunga matahari (Helianthus annuus L.) Asmi Ode; Fauzia Hulopi; Pebrywati Watimury; Sriyati Sampulawa; Farida Bahalwan; Wa Nirmala; Abdullah Derlean
Jurnal Agrohut Vol 14 No 2 (2023): Agrohut
Publisher : Fakultas Pertanian Universitas Darussalam Ambon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51135/agh.v14i2.262

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian larutan tembaga terhadap pertumbuhan tanaman bunga matahari (H. annuus L.). Penelitian ini dilakukan dengan menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari 4 konsentrasi perlakuan logam Cu yaitu P0: 0 mg/L, P1: 100 mg/L, P2: 200 mg/L dan P3: 500 mg/L, dengan 5 ulangan untuk setiap perlakuan sehingga didapatkan 20 sampel tanaman. Penelitian ini dilakukan dengan merendam biji bunga matahari kultivar dalam wadah berisi air hangat selama semalam kemudian ditanam pada polybag. Tanaman dipelihara secara intensif hingga berumur 2 minggu setelah tanam dan dilakukan aplikasi Cu. Logam Cu yang digunakan berupa garam CuSO4.5H2O. Pemberian larutan Cu dilakukan setelah tanaman mengalami aklimatisasi selama 1 minggu (tanaman berumur ± 5 minggu). Cu yang diaplikasikan berupa garam CuSO4.5H2O yang mengandung kurang lebih 25% ion Cu. Garam tersebut dilarutkan dalam aquades sebanyak 1L sesuai konsentrasi dan diberikan 4 kali dalam jangka waktu 2 minggu hingga larutan Cu habis. Larutan Cu sebanyak 250 ml disiramkan ke tanah disekitar tanaman secara hati-hati agar tidak kontak langsung dengan batang tanaman. Pada perlakuan kontrol tidak dilakukan penyiraman garam CuSO4.5H2O. Setelah pemberian Cu, dilakukan pengamatan dan pengukuran parameter pertumbuhan. Parameter pertumbuhan terdiri dari tinggi tanaman, jumlah daun, dan panjang akar tanaman. Data hasil pengamatan disajikan dalam bentuk gambar dan pengukuran akan dianalisis menggunakan analisis sidik ragam (ANAVA) dan diuji lanjut dengan DMRT (Duncan Multiple Range Test) dengan tingkat kepercayaan 95%. Hasil penelitian menunjukkan bahwa pemberian larutan Cu berpengaruh terhadap pertumbuhan tanaman bunga matahari. Semakin tinggi konsentrasi Cu mengakibatkan terhambatnya tinggi tanaman, penurunan jumlah daun, dan terhambatnya pemanjangan akar tanaman.
Asesmen Autentik Setting Pembelajaran Berdiferensasi Dalam Pembelajaran Jarak Jauh Mewujudkan Merdeka Belajar SMP PGRI Mawah kasliyanto, kasliyanto; Sampulawa, Sriyati; Darmawati, Siti; Zurimi, Safarin
JIKAP PGSD: Jurnal Ilmiah Ilmu Kependidikan Vol 6, No 2 (2022): Mei (JIKAP PGSD)
Publisher : Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26858/jkp.v6i2.33308

Abstract

The purpose of the research is for the development of authentic assessments of differentiated learning settings in distance learning to realize legitimate, reliable, objectiv and practical learning freedom. The Development Model of the assessment tool used in this study refers to the 4-D model consisting of defining, designing, developing, and deploying as discovered by Thiagarajan. The subjects in this study included 40 students of class VII of PGRI Mawah Junior High School. The trial was conducted using a pre-experimental design type one-shot case study. Data analysis techniques use qualitative descriptive. Instrument validation by experts and education practitioners using check list sheets with a likert scale of 1 (very lacking) – 4 (excellent). The test before the trial uses the validity formula of Gregory's contents, while the validity test after the trial (empirical validity) uses SPSS software with bivariate pearson analysis. Test the reliability, objectivity and practicality of the instrument using SPSS. Authentic assessment of differentiated learning settings in distance learning to realize independent learning both use guidelines and authentic assessment tools and instruments meet valid, reliable, objective and practical criteria for use because they have gone through revisions and trials.
Efektivitas Ekstrak Alga Coklat Padina australis dalam Menghambat Pertumbuhan Bacillus cereus dan Escherichia coli Sampulawa, Sriyati; Ode, Asmi; Hulopi, Fauzia; Nirmala, Wa; Bahalwan, Farida; Darmawati, Siti
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.13225

Abstract

This study aims to determine the effectiveness of the brown algae extract Padina australis in inhibiting the growth of Bacillus cereus and Eschericia coli bacteria. Samples were taken from the waters of Osi Island, West Seram, Maluku Province as much as 1 kg, the sample was then dried using the sundrying method. The dried sample was then soaked for 24 hours and extracted. The extract results were then tested for phytochemicals and antibacterial activity tests carried out in the Microbiology Laboratory of Pattimura University, Ambon. The results showed that (1) The brown algae extract Padina australis contains secondary metabolites in the form of flavonoids, terpenoids, steroids, saponins and alkaloids; (2) The combination of these various compounds provides a synergistic effect that can increase the effectiveness of antimicrobials in inhibiting the growth of Bacillus cereus and Escherichia coli bacteria; (3) The minimum inhibitory concentration (MIC) of Padina australis algae extract has different abilities in inhibiting test bacteria. The MIC of Bacillus cereus bacteria at a concentration of 10% has an inhibition zone of 7 mm and the MIC of Escherichia coli bacteria at a concentration of 60% has an inhibition zone of 11 mm.
Co-Authors Abdullah Derlean Asmi Ode Asmi Ode Farida Bahalwan Fauzia Hulopi Fauzia Hulopi Kasliyanto Kasliyanto, Kasliyanto Nirmala, Wa Ode, Asmi Rizky Fatmalasari. L Safarin Zurimi Siti Darmawati Wa Nirmala Watimury, Pebrywati