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All Journal Jurnal Sain Veteriner JURNAL PENGABDIAN KEPADA MASYARAKAT JIKAP PGSD: Jurnal Ilmiah Ilmu Kependidikan Jurnal Agrohut Bioscientist : Jurnal Ilmiah Biologi
Sriyati Sampulawa
Universitas Darussalam Ambon
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Education Engineering Immunology & microbiology Languange, Linguistic, Communication & Media Social Sciences Veterinary

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Journal : Bioscientist : Jurnal Ilmiah Biologi

 1 
Identifikasi Senyawa Bioaktif Ekstrak Alga Coklat (Hormophysa triquetra) Sriyati Sampulawa; Farida Bahalwan
Bioscientist : Jurnal Ilmiah Biologi Vol 10, No 1 (2022): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v10i1.4918

Abstract

Hormophysa triquetra is a type of brown algae that is easily found along the coast with sandy and rocky sediments. Based on the results of research, some brown algae are known to be often used because they are a rich source of various secondary metabolites. Hormophysa triquetra is a type of brown algae that needs to be investigated for the content of secondary metabolites so that it can be used widely. The research method used is an experimental method with sampling techniques carried out using free samples, which were carried out in the waters of Hutumuri Village, Ambon City, the samples obtained were then dried by aerating, then the algae samples were extracted by the maceration method, then the results of the Hormophysa extract triquetra carried out phytochemical tests. Phytochemical test of Hormophysa triquetra extract included examination of the content of alkaloids, flavonoids, tannins, terpenoids/steroids and saponins. The test results showed that the Hormophysa triquetra algae extract contained flavonoid, terpenoid, phenolic, saponin and alkaloid compounds.
Pemanfaatan Ekstrak Kunyit (Curcuma domestica Val.) sebagai Pewarna Alami Pembuatan Preparat Awetan Jaringan Tumbuhan dalam Praktikum Biologi Sel Asmi Ode; Sriyati Sampulawa; Fauzia Hulopi
Bioscientist : Jurnal Ilmiah Biologi Vol 10, No 2 (2022): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v10i2.5979

Abstract

Making preparations for observing cells or tissues in practicum activities requires dyes. In cell biology practicum, especially observation of plant tissue in the Department of Biology Education, FKIP, Ambon University of Darussalam, it is still very limited, because it does not have chemical dyes for making preserved preparations. This is because chemical dyes are quite expensive, unsafe, and will spoil if stored for a long time, so it is very important to make natural dyes as a substitute for synthetic dyes so that the preserved preparations produced are safer, more affordable, and can be used for a long time. which is old. Sources of natural dyes are obtained from plant parts, for example: roots, bark, leaves, fruit, wood, seeds, and flowers. Turmeric (Curcuma domestica Val.) is a natural dye that can be used, because it is relatively cheap, easy to find, non-carcinogenic, and biodegradable. The active pigments in turmeric which can color plant tissue and give it a yellow color are curcuminoids. The aim of this research was to study the utilization of turmeric extract (Curcuma domestica Val.) as a natural dye for preparing preserved preparations of plant tissues in cell biology practicum. This type of research is experimental research, which has several stages of research including: observation, preparation, research implementation, and analysis stage. At the data analysis stage, a qualitative descriptive method was used to determine the quality of the preserved preparations. The quality of the preparations in this study was expressed by the percentage of eligibility of ≥ 62.5% or feasible criteria. The results showed that the average percentage of the quality of the preparations that had been validated was 66.96% and 67.85% with proper criteria. This shows that the preserved preparations made are suitable for observing plant tissues in cell biology labs.
Efektivitas Ekstrak Alga Coklat Padina australis dalam Menghambat Pertumbuhan Bacillus cereus dan Escherichia coli Sampulawa, Sriyati; Ode, Asmi; Hulopi, Fauzia; Nirmala, Wa; Bahalwan, Farida; Darmawati, Siti
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.13225

Abstract

This study aims to determine the effectiveness of the brown algae extract Padina australis in inhibiting the growth of Bacillus cereus and Eschericia coli bacteria. Samples were taken from the waters of Osi Island, West Seram, Maluku Province as much as 1 kg, the sample was then dried using the sundrying method. The dried sample was then soaked for 24 hours and extracted. The extract results were then tested for phytochemicals and antibacterial activity tests carried out in the Microbiology Laboratory of Pattimura University, Ambon. The results showed that (1) The brown algae extract Padina australis contains secondary metabolites in the form of flavonoids, terpenoids, steroids, saponins and alkaloids; (2) The combination of these various compounds provides a synergistic effect that can increase the effectiveness of antimicrobials in inhibiting the growth of Bacillus cereus and Escherichia coli bacteria; (3) The minimum inhibitory concentration (MIC) of Padina australis algae extract has different abilities in inhibiting test bacteria. The MIC of Bacillus cereus bacteria at a concentration of 10% has an inhibition zone of 7 mm and the MIC of Escherichia coli bacteria at a concentration of 60% has an inhibition zone of 11 mm.
Efektivitas Ekstrak Alga Coklat Padina australis dalam Menghambat Pertumbuhan Bacillus cereus dan Escherichia coli Sampulawa, Sriyati; Ode, Asmi; Hulopi, Fauzia; Nirmala, Wa; Bahalwan, Farida; Darmawati, Siti
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.13225

Abstract

This study aims to determine the effectiveness of the brown algae extract Padina australis in inhibiting the growth of Bacillus cereus and Eschericia coli bacteria. Samples were taken from the waters of Osi Island, West Seram, Maluku Province as much as 1 kg, the sample was then dried using the sundrying method. The dried sample was then soaked for 24 hours and extracted. The extract results were then tested for phytochemicals and antibacterial activity tests carried out in the Microbiology Laboratory of Pattimura University, Ambon. The results showed that (1) The brown algae extract Padina australis contains secondary metabolites in the form of flavonoids, terpenoids, steroids, saponins and alkaloids; (2) The combination of these various compounds provides a synergistic effect that can increase the effectiveness of antimicrobials in inhibiting the growth of Bacillus cereus and Escherichia coli bacteria; (3) The minimum inhibitory concentration (MIC) of Padina australis algae extract has different abilities in inhibiting test bacteria. The MIC of Bacillus cereus bacteria at a concentration of 10% has an inhibition zone of 7 mm and the MIC of Escherichia coli bacteria at a concentration of 60% has an inhibition zone of 11 mm.
Co-Authors Abdullah Derlean Asmi Ode Asmi Ode Farida Bahalwan Fauzia Hulopi Fauzia Hulopi Kasliyanto Kasliyanto, Kasliyanto Nirmala, Wa Ode, Asmi Rizky Fatmalasari. L Safarin Zurimi Siti Darmawati Wa Nirmala Watimury, Pebrywati