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All Journal Jurnal Kebidanan Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Research Journal of Life Science
Sumarno Reto Prawiro
Department Of Microbiology, Medical Faculty, Brawijaya University, Malang, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Medicine & Pharmacology Nursing

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Journal : Research Journal of Life Science

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Turmeric (Curcuma longa) Decoctum Had no Effect on Vascular Endothelial Growth Factor (VEGF) and Vascular Endothelial Cadherin (VE-cadherin) Expression in the Chick Embryo Sri Winarsih; I Wayan Arsana Wiyasa; Sri Andarini; Sumarno Reto Prawiro
Research Journal of Life Science Vol 5, No 2 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (362.581 KB) | DOI: 10.21776/ub.rjls.2018.005.02.3

Abstract

Turmeric (Curcuma longa) as a medicinal plant has widely consumed by pregnant women. The absence of dose standardization and the regulation of turmeric consumption in pregnancy raise concerns, especially if taken in early pregnancy. Angiogenesis is the process of new blood vessel formation from previously existing blood vessels, and plays an important role in embryogenesis and placentation during pregnancy. Angiogenesis is regulated by angiogenic molecules, such as VEGF and VE-cadherin. Turmeric has antiangiogenic effects in which the crude extract is pharmacologically more potent compared to the pure curcumin form. This study aimed to determine whether a turmeric decoctum affects the expression of VEGF and VE-cadherin in chick embryos. Turmeric was extracted by the decoction and freeze dried methods to obtain turmeric decoctum powder. This was tested on embryonated chicken eggs, which were divided into four groups; control group (2% DMSO) and treatment groups receiving various doses of the turmeric decoctum (200 ppm, 300 ppm and 400 ppm). The eggs used were less than 7 days after oviposition and incubated for 16 hours prior to injection of the treatment solution in ovo to the center of the yolk, followed by reincubation for up to 48 hours. Intraembryonic VEGF and VE-cadherin expression were assessed by whole-mount immunohistochemistry and quantified using image analysis techniques. This study showed administration of turmeric decoctum up to 400 ppm had no effect on the expression of VEGF and VE-cadherin in chick embryos.
Nigella sativa Extract Increases Antibacterial Activity by Up-Regulating T-reg and Th2 Levels in Salmonella enterica subsp. enterica serovars Typhimurium-Exposed Balb/c Mice Zahra Ahmed; Sumarno Reto Prawiro; Agustina Tri Endharti
Research Journal of Life Science Vol 5, No 1 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (505.27 KB) | DOI: 10.21776/ub.rjls.2018.005.01.2

Abstract

Nigella sativa extract (NSE) is known to be an effective antibacterial and immuno-modulating agent. This study aimed to demonstrate that treatment of Salmonella eterica subsp. eterica serovars typhimurium-infected mice with NSE would cause an increase in T-regulatory (T-reg) and Th2 cells and a decrease in the number of spleen bacterial colonies. Mice were randomly divided into five groups [control (T0), mice infected with S. typhimurium only (T1), and mice infected with S. typhimurium and then treated with NSE 0.52, 5.2, or 52 mg/kg body weight NSE (N1, N2, and N3, respectively)]. Levels of T-reg and Th2 cells were determined via flow cytometry and the number of spleen bacterial colonies was determined by observation. The results showed that treatment with NSE 0.52, 5.2, and 52 mg/kg significantly increased the number of T-reg and Th2 cells (p<0.05) relative to T0. Administration of NSE 5.2 mg/kg increased the number of T-reg cells, while administration of NSE 52 mg/kg caused the greatest increase in the number of Th2 cells in the spleens of S. typhimurium-infected mice. Moreover, no S. typhimurium colonies were found in the spleens of any NSE-treated mice. Our results suggest that NSE has therapeutic potential to ameliorate S. typhimurium infection.
Co-Authors Agustina Tri Endharti Alvi Milliana Aulanni'am Aulanni'am Avin Ainur Fitrianingsih Edi Widjajanto Ema Dianita Mayasari Erni Yudaningtyas Hamid Hunaif Dhofi Alluza Lailia Nur Rachma Mas Mansyur Nur Azizah Sanarto Santoso Sri Andarini Sri Winarsih Tinny Endang Hernowati Tri Yudani Mardining Raras Umu Qonitun Wiyasa, I Wayan Arsana Zahra Ahmed