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PROTEIN ADHESI SUB UNIT PILI Shigella flexneri 18 kDa MENINGKATKAN EKSPRESI PROTEIN ANTIMIKROBA ï¢ DEFENSIN PADA MENCIT ( STRATEGI PENGEMBANGAN VAKSIN SHIGELLOSIS BERBASIS PROTEIN ADHESI) Fitrianingsih, Avin Ainur; Rachma, Lailia Nur; Milliana, Alvi
Journal of Islamic Medicine Vol 2, No 2 (2018): Journal of Islamic Medicine
Publisher : Faculty of Medicine and Health Science, Universitas Islam Negeri Maulana Malik Ibrahim

Shigellosis merupakan penyakit endemis yang terutama terjadi di negara berkembang dan merupakanÂ penyebab tersering penyakit diare yang berdarah. Pengembangan vaksin dari molekul adhesin memiliki kelebihan pada reaksi imun tubuh yang terbentuk akan membentuk sistem pertahanan tubuh yang lebih kuat dalam mengeliminasi bakteri serta tidak menimbulkan panas seperti vaksin dari sel utuh bakteri atau dari LPS-nya. Berdasarkan penelitian sebelumnya diketahui bahwa pili Shigella flexneri dengan Berat Molekul (BM) 18 kDa merupakan molekul adhesi serta mampu bereaksi silang dengan pili Shigella spesies lainnya. Penelitian ini dimulai dengan proses isolasi pili S.Â flexneri, kemudian dilanjutkan dengan profiling pili dengan cara elektroforesis SDS-PAGE. Kemudian dilakukan perbanyakan protein sub unit pili dengan elektroelusi. Protein sub unit yang sudah diperoleh kemudian diimunisasikan ke mencit Balb/c secara peroral menggunakan sonde, dengan pemberian Â 4 kali selang 1 minggu, dan pada akhir minggu ke 4 mencit dimatikan. Serum, mukosa usus dan paru mencit yang didapatkan kemudian diuji kadar Antimicrobial peptideÂ Î² defensin dengan metode ELISA. Kesimpulan penelitian ini adalah pemberian protein adhesi sub unit piliÂ S. flexneri 18 kDa selama 4 minggu mampu meningkatkan kadar Antimicrobial peptide Î² defensin pada serum, mukosa usus, dan paru mencit. Sehingga protein adhesi sub unit pili S. flexneri 18Â kDa berpotensi menjadi vaksin berbasis sub unit protein adhesi untuk kasus Shigellosis.Kata Kunci : Shigella flexneri, sub unit pili 18 kDa, Antimicrobial peptideÂ Î² defensinÂ

Cross Reaction among Antibody Pili sub unit Hemagglutinin Proteins and Outer Membrane sub unit Hemagglutinin Proteins of Shigella flexneri Fitrianingsih, Avin Ainur; Rachma, Lailia Nur; Milliana, Alvi; Hernowati, Tinny Endang; Aulanni'am, Aulanni'am; Santoso, Sanarto; Prawiro, Sumarno Reto
Journal of Tropical Life Science Vol 7, No 1 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.01.01

Shigella Â flexneri is the most common causal agent of shigellosis. Its pili are composed of pili protein subunits. Adhesion molecules can be found on the pili and outer membrane proteins (Omp). A hemagglutination reaction can be used for screening of adhesion molecules. Objectives: The purpose of this study was to determine the molecular weight of the pili protein subunits and outer membrane proteins of S. flexneri that act as hemagglutinin proteins, and to prove whether there is a cross-reaction between antibodies against hemagglutinin pili protein subunits and outer membrane proteins of S. flexneri. Methods: Pili protein subunits were isolated using pili bacteria cutters, and the outer membrane proteins were solubilized and obtained using sodium dodecyl sulfate 0.05% as detergent for Omp isolation. Â The hemagglutination reaction used mice erythrocytes. The cross reactions between subunit pili proteins were conducted by Western blot and Dot blot. Results:. Antibodies against hemagglutinin sub unit pili protein 18 kDa responded to pili protein subunits 18 kDa; 23 kDa; 34 kDa; and 53 kDa; and Omp 23 kDa and 27 kDa. Omp and subunit pili proteins S. flexneri consists of several identical epitopes Â that were responsible for the similarity of the response profile in the cross-reactions of antibodies.Â

APLIKASI SITOGENETIKA UNTUK MENDETEKSI ABNORMALITAS KROMOSOM PADA Benign Barrettâ€™s EPITHELIUM YANG MENGALAMI TRANSFORMASI MALIGNA Milliana, Alvi
El-Hayah : Jurnal Biologi Vol 4, No 2 (2014): EL-HAYAH (Vol 4, No 2, Maret 2014)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v4i2.2626

Barrettâ€™s esophagus is a metaplastic alteration of the normal distal esophageal epithelium. Barrettâ€™s esophagus is present in 10%â€“20% of patients with gastroesophageal reflux disease (GERD). Its major significance is as a predisposing factor for esophageal adenocarcinoma, which carries a high mortality rate and a rapidly growing incidence in the United States. Â Esophageal adenocarcinoma carries a grave prognosis, with a relative 3-year survival rate of only 20% in the United States from 1995â€“1998. Â Cancer arises in BE through a multistep sequence of events initiated by chronic GERD that leads to metaplasia, dysplasia, andÂ adenocarcinoma sequence. Primary pathogenetic changes in cancer result from balanced rearrangements of chromosome. Losses of chromosomes 4, 18, 21, and Y were the most frequent numeric changes. The chromosome arms most frequently rearranged were 1p, 3q, 11p and 22p. Evidence of chromosomal rearrangements [t(10;16) and dup (11q)] appeared during the early stages of the BEC model. After further validation, in-vivo, they may be clinically useful for diagnosis of BE, progressing to dysplasia or esophageal adenocarcinoma

DETEKSI VIRUS HEPATITIS A DAN B BERDASARKAN PATOGENESA DAN STRUKTUR VIRUS Milliana, Alvi
El-Hayah : Jurnal Biologi Vol 3, No 2 (2013): EL-HAYAH (VOL 3, NO 2, Maret 2013)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v3i2.2614

Hepatitis virus merupakan masalah kesehatan global yang menyebabkan mortalitas dan morbiditas jutaan orang di dunia, baik dewasa maupun anak-anak. Hepatitis virus akut terbanyak disebabkan oleh virus hepatitis A, sedangkan virus hepatitis B menyebabkan hepatitis akut dan kronis. Diagnosa hepatitis virus ditegakkan berdasarkan pemeriksaaan laboratorium karena secara klinis banyak kasus yang tidak menunjukkan gejala. Deteksi virus hepatitis dilakukan secara langsung dengan pemeriksaan antigen virus, maupun secara tidak langsung dengan pemeriksaan antibody virus. Deteksi ini dilakukan berdasarkan patogenesa virus dan struktur virus hepatitis A dan B. Deteksi ini bermanfaat untuk mengetahui perjalanan penyakitdan status infeksius penderita

ISOLASI DAN IDENTIFIKASI BAKTERI ENDOFIT RIMPANG TEMULAWAK (Curcuma xanthorrhiza Roxb.) SEBAGAI PENGHASIL SENYAWA ANTIFUNGI TERHADAP Candida albicans Milliana, Alvi; Safitri, Wahyu
El-Hayah : Jurnal Biologi Vol 5, No 2 (2015): EL-HAYAH (VOL 5, NO 2, Maret 2015)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v5i2.3020

Infectious diseases in humans by microbes in developing countries including Indonesia remains a major problem. Forty-three percent of deaths in developing countries are caused by infections. Candida is a yeast species that often cause opportunistic infections. Disease caused by Candida can affect the mouth, vagina, skin, nails, lungs, sometimes can cause septicemia, endocarditis and meningitis. Rising yeast-leavened which has resistance to antifungal compounds that exist, encourage researchers continue to discover new compounds which have antifungal properties. In addition, the side effects caused by the use of antifungal drugs, causing people still expect the natural ingredients that can be used as an alternative treatment. According to WHO, 80% of the world's population still relies on traditional medicine, including the use of drugs from plants, one of which is the use of turmeric plant (Curcuma zanthorriza). Bioactive compounds of plants that are antifungal generally are volatile oil, aldehyde and phenol compounds. The existence of endophytic microbes in plants have economic significance for the industry favorable treatment. Endophytic microbes easily grown, have a shorter life cycle than the plant and capable of producing bioactive compounds similar to the host plant. Therefore, it is necessary to study the potential of endophytic bacteria ginger rhizome (C. xanthorrhiza) as a producer of Candida albicans antifungal compounds. This study uses ginger rhizome obtained from Stone and Pasuruan. After the isolation of microbes from ginger rhizome, further purification by means of subcultures. The endophytic microbial isolates gram staining and identification of bacteria. The result is a gram-positive bacterium Actinomyces viscosus, Bacillus brevis gram-positive bacteria and gram-negative bacteria Pseudomonas stutzeri. Then do the production of antifungal metabolites produced by endophytic bacteria and C. albicans antifungal test Paper Disc Diffusion method. The result looks the inhibition zone with a diameter of 4 mm, 15 mm and 45 mm

Perbandingan Uji Aktivitas Antibakteri Umbi Bawang Merah (Allium Cepa L) Dan Umbi Bawang Putih (Allium Sativum) Terhadap Pertumbuhan Bakteri Shigella Dysenteriae Purnomo S, Fahrurrozi Hari; Fitrianingsih, Avin Ainur; Rahmah, Zainabur; Milliana, Alvi
Journal of Islamic Medicine Vol 4, No 2 (2020): JOURNAL OF ISLAMIC MEDICINE EDISI SEPTEMBER 2020
Publisher : Faculty of Medicine and Health Science, Universitas Islam Negeri Maulana Malik Ibrahim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/jim.v4i2.10169

Background :Diarrhea is one of the causes of morbidity and mortality in infants and children around the world. One of the causes of diarrhea is Shigella sp bacteria or shigellosis. Nowadays, many antibiotics resistant to the Shigella sp bacteria are one example of chloramphenicol, so it is necessary to have other alternatives such as natural ingredients. Objective: Determine the activity of the red onion (Allium cepa L) and garlic (Allium sativum), which is known to possess antibacterial phytochemical compounds against the Shigella dysenteriae bacteria. Method: This research is a laboratory experimental study to determine antibacterial activity using the Kirby-Bauer or diffusion method of discs and to determine the presence of Minimum Inhibitory Concentration (MIC) and Minimum Bacterial Concentration (MBC). The study uses five treatments i.e., onion extracts and garlic extracts with a concentration of 30%, 40%, 50%, 60%, 70%, control positive (Ciprofloxacin), and negative control (aquadest). Results: Red onion diffusion has the best antibacterial activity at concentrations 70% while garlic has the best antibacterial activity at a concentration of 70%. In this study, there were no MIC and MBC. The MIC data analyzed with the crucial test of Kruskal-Wallis (p 0.05) i.e., in onion extract p = 0.003 and in garlic P = 0.003, which means significant. The MBC Data is analyzed by crucial test (P 0.05) in the onion extract p = 0.003 and on garlic p = 0.04. Conclusion : the disc diffusion and the MBC can be concluded that garlic extract can inhibit the growth of S. dysenteriae compared to red onion extracts. onion extracts).

High Degree of Parasitemia Induces Placental Hypoxia and Low Birth Weight Nurfianti Indriana; Zainabur Rahmah; Alvi Milliana
Journal of Agromedicine and Medical Sciences Vol 8 No 2 (2022)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v8i2.31243

Placental malaria causes low fetal weight associated with infiltration of monocytes and parasites in the placenta resulting in placental hypoxia. Hypoxia is characterized by HIF expression. HIF-1Î± expression responds early to the occurrence of hypoxia (<24 hours) and HIF-2Î± promotes chronic hypoxia (>24 hours). There were two groups in this study, namely the control group (10 pregnant mice without Plasmodium berghei infection) and the treatment group (10 pregnant mice infected with Plasmodium berghei). Pregnant mice were operated on on the 18th day after mating. The degree of parasitemia was measured by Giemsa staining. Expression of HIF-1Î± and HIF-2Î± in placental tissue was measured by immunohistochemistry. Results by paired t test, HIF-1Î± expression in the placental tissue in the treatment group was higher than the control group (p=0.02), HIF-2Î± expression in the placental tissue was higher in the treatment group than in the control group (0, 01) the fetal weight in the treatment group was lower than in the control group (p=0.01). By using SEM analysis, the degree of parasitemia caused high expression of HIF-1Î± in placental tissue (tcount = 4.625, ttable = 1.96). High expression of HIF-2Î± in placental tissue (tcount = 2.672 ttable = 1.96). The degree of parasitemia causes low fetal weight (tcount = 27.764 ttable = 1.96). The results also showed that HIF-1 caused low birth weight (tcount = 2.376 ttable=1.96) also HIF-2Î± caused low fetal weight (tcount = 4.267 â‰¥ 1.96). Conclusions the degree of parasitemia causes high expression of HIF-1Î± and HIF-2Î± in placental tissue and low birth fetuses. Expression of HIF-1Î± and HIF-2 also causes low birth weight. Keywords: malaria in pregnancy, degree of parasitemia, HIF-1Î± , HIF-2Î± , birth weight

Cross Reaction among Antibody Pili sub unit Hemagglutinin Proteins and Outer Membrane sub unit Hemagglutinin Proteins of Shigella flexneri Avin Ainur Fitrianingsih; Lailia Nur Rachma; Alvi Milliana; Tinny Endang Hernowati; Aulanni'am Aulanni'am; Sanarto Santoso; Sumarno Reto Prawiro
Journal of Tropical Life Science Vol. 7 No. 1 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.01.01

Shigella Â flexneri is the most common causal agent of shigellosis. Its pili are composed of pili protein subunits. Adhesion molecules can be found on the pili and outer membrane proteins (Omp). A hemagglutination reaction can be used for screening of adhesion molecules. Objectives: The purpose of this study was to determine the molecular weight of the pili protein subunits and outer membrane proteins of S. flexneri that act as hemagglutinin proteins, and to prove whether there is a cross-reaction between antibodies against hemagglutinin pili protein subunits and outer membrane proteins of S. flexneri. Methods: Pili protein subunits were isolated using pili bacteria cutters, and the outer membrane proteins were solubilized and obtained using sodium dodecyl sulfate 0.05% as detergent for Omp isolation. Â The hemagglutination reaction used mice erythrocytes. The cross reactions between subunit pili proteins were conducted by Western blot and Dot blot. Results:. Antibodies against hemagglutinin sub unit pili protein 18 kDa responded to pili protein subunits 18 kDa; 23 kDa; 34 kDa; and 53 kDa; and Omp 23 kDa and 27 kDa. Omp and subunit pili proteins S. flexneri consists of several identical epitopes Â that were responsible for the similarity of the response profile in the cross-reactions of antibodies.Â

NEUTRAL PH AND COLD TEMPERATURES STABILIZE THE PROTECTIVE EFFECT OF CRUDE PILI S. FLEXNERI ON THE ILEAL MUCOSA OF MICE Avin Ainur Fitrianingsih; Lailia Nur Rachma; Alvi Milliana
Jurnal Neutrino:Jurnal Fisika dan Aplikasinya Vol 12, No 1 (2019): October
Publisher : Department of Physics, Maulana Malik Ibrahim State Islamic University of Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/neu.v12i1.15525

Alternative therapies are being sought as a result of the rising concerns with inadequate and untrustworthy medical treatments for Shigella flexneri. The current study aimed to assess the preventive and therapeutic effects of crude pili S. flexneri on of neutral pH and cold temperature against Shigella flexneri infection in immunocompetent mice. Twenty male Swiss albino mice were randomly assigned to one of two groups: control or experimental groups. Each group was subsequently separated into four equal subgroups. In order to infect the mice, 4 weeks before infection, the experimental subgroups were given crude pili S. flexneri orally in every week (three times) until the completion of the research. After following the treatment of crude pili S. flexneri, all group infected with Shigella flexneri. The small intestines of mice were processed and analyzed for the presence of the pathological lesions. Jejunal portions were measured. The findings revealed that vaccinated mice had a statistically significant increase in the quantity of health mucose as compared to non-infected group in ileal sections. Crude pili S. flexneri was administered to the intestinal portions of all subgroups before or after the infection, and the architecture was found to be more or less normal. Our data indicate that crude pili S. flexneri in neutral pH and cold temperature is a useful preventative and a potentially effective treatment medication for S. flexneri infection.

Association Between Stress Level, Sleep Quality, Physical Activity With Cardiorespiratory Fitness In Medical Students: A Cross Sectional Study Ermin Rachmawati; Alvi Milliana; Sefira Maulida Filzah; Adhitya Wishnu Kresnanda
Journal of Islamic Medicine Vol 7, No 1 (2023): JOURNAL OF ISLAMIC MEDICINE EDISI MARET 2023
Publisher : Faculty of Medicine and Health Science, Universitas Islam Negeri Maulana Malik Ibrahim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/jim.v7i1.20429

Introduction Modern life style and high burden of academic tasks contribute to the upsurge of stress, poor sleep quality, lack of sufficient physical activity in medical student society nowadays. The unhealthy life style act as one cardiovascular risk factor which can be predicted using Cardiorespiratory Fitness (CRF) examination. Objectives to identify the association between stress, sleep quality, physical activity with CRF level among medical students. Design The cross-sectional data was collected between September until November 2021. A total of 38 male and 73 female students of Faculty of Medicine and Health Sciences UIN Maulana Malik Ibrahim Malang were participated in this study. Stress level was assessed with DASS-21, sleep quality with PSQI, physical activity with GPAQ questionnaire. The level of CRF was measured using the Harvard Step Test. Statistical analysis for bivariate correlation ordinal scale was tested using Spearman Rank. Results 83 participants (74,8%) had low CRF. Meanwhile, 76 participants (68,5%) experienced no stress. In addition, 75 participants (67,6%) had poor sleep quality. A total number of 79 participants (71%) had low physical activity. There was no significant correlation between stress level, sleep quality, physical activity with CRF ( p=0,962; 0,772; 0,114, respectively). The participants who had low physical activity and low CRF was found in 56 participants (50,45%). Conclusions There was no association between stress level, sleep quality, physical activity with CRF among medical students. Keywords: Cardiorespiratory fitness; Stress; Sleep quality; Physical activity.

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