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PROTEIN ADHESI SUB UNIT PILI Shigella flexneri 18 kDa MENINGKATKAN EKSPRESI PROTEIN ANTIMIKROBA ï¢ DEFENSIN PADA MENCIT ( STRATEGI PENGEMBANGAN VAKSIN SHIGELLOSIS BERBASIS PROTEIN ADHESI) Fitrianingsih, Avin Ainur; Rachma, Lailia Nur; Milliana, Alvi
Journal of Islamic Medicine Vol 2, No 2 (2018): Journal of Islamic Medicine
Publisher : Faculty of Medicine and Health Science, Universitas Islam Negeri Maulana Malik Ibrahim

Shigellosis merupakan penyakit endemis yang terutama terjadi di negara berkembang dan merupakanÂ penyebab tersering penyakit diare yang berdarah. Pengembangan vaksin dari molekul adhesin memiliki kelebihan pada reaksi imun tubuh yang terbentuk akan membentuk sistem pertahanan tubuh yang lebih kuat dalam mengeliminasi bakteri serta tidak menimbulkan panas seperti vaksin dari sel utuh bakteri atau dari LPS-nya. Berdasarkan penelitian sebelumnya diketahui bahwa pili Shigella flexneri dengan Berat Molekul (BM) 18 kDa merupakan molekul adhesi serta mampu bereaksi silang dengan pili Shigella spesies lainnya. Penelitian ini dimulai dengan proses isolasi pili S.Â flexneri, kemudian dilanjutkan dengan profiling pili dengan cara elektroforesis SDS-PAGE. Kemudian dilakukan perbanyakan protein sub unit pili dengan elektroelusi. Protein sub unit yang sudah diperoleh kemudian diimunisasikan ke mencit Balb/c secara peroral menggunakan sonde, dengan pemberian Â 4 kali selang 1 minggu, dan pada akhir minggu ke 4 mencit dimatikan. Serum, mukosa usus dan paru mencit yang didapatkan kemudian diuji kadar Antimicrobial peptideÂ Î² defensin dengan metode ELISA. Kesimpulan penelitian ini adalah pemberian protein adhesi sub unit piliÂ S. flexneri 18 kDa selama 4 minggu mampu meningkatkan kadar Antimicrobial peptide Î² defensin pada serum, mukosa usus, dan paru mencit. Sehingga protein adhesi sub unit pili S. flexneri 18Â kDa berpotensi menjadi vaksin berbasis sub unit protein adhesi untuk kasus Shigellosis.Kata Kunci : Shigella flexneri, sub unit pili 18 kDa, Antimicrobial peptideÂ Î² defensinÂ

Respon Jamaah Haji terhadap Kualitas Pelayanan Kesehatan di Daerah Kecamatan Pademawu Kabupaten Pamekasan Rahmah, Zainabur; Fitrianingsih, Avin Ainur; Rachma, Lailia Nur; Astari, Lina Fitria; Riskiyana, Riskiyana
Journal of Islamic Pharmacy Vol 5, No 1 (2020): J. Islamic Pharm.
Publisher : Universitas Islam Negeri Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/jip.v5i1.9487

The number of pilgrims of various ages and levels of education experience many health problems such as hypertension, respiratory infections and heart problems. From these problems encourage researchers to determine the response of prospective pilgrims to health services in Pademawu Pamekasan. The object of this study is the pilgrims in Pademawu Pamekasan Regency. The purpose of this study was to determine the response of the pilgrims in Pademawu District, Pamekasan Regency. This research uses a quantitative approach that is data collection using a questionnaire instrument. The sampling technique uses a total sampling population of 70 pilgrims. The study was conducted in the District of Pademawu Pamekasan to the Hajj pilgrims in 2018. The results of the study of the pilgrims' response to the quality of service include cognitive, affective, conative, age and education levels. Overall, the response of the haj pilgrims to the quality of service on the cognitive aspect was 29.5%. In the affective aspect of 4.69%. In the conative aspect 2.05%. At the age factor of 4.51% and at the aspect of Education level as much as 2.19%. The quality of health services affects the response of the pilgrims to the cognitive, affective and conative aspects but is not influenced by the age and level of education of the pilgrims.Keywords: hajj response, service, health

Cross Reaction among Antibody Pili sub unit Hemagglutinin Proteins and Outer Membrane sub unit Hemagglutinin Proteins of Shigella flexneri Fitrianingsih, Avin Ainur; Rachma, Lailia Nur; Milliana, Alvi; Hernowati, Tinny Endang; Aulanni'am, Aulanni'am; Santoso, Sanarto; Prawiro, Sumarno Reto
Journal of Tropical Life Science Vol 7, No 1 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.01.01

Shigella Â flexneri is the most common causal agent of shigellosis. Its pili are composed of pili protein subunits. Adhesion molecules can be found on the pili and outer membrane proteins (Omp). A hemagglutination reaction can be used for screening of adhesion molecules. Objectives: The purpose of this study was to determine the molecular weight of the pili protein subunits and outer membrane proteins of S. flexneri that act as hemagglutinin proteins, and to prove whether there is a cross-reaction between antibodies against hemagglutinin pili protein subunits and outer membrane proteins of S. flexneri. Methods: Pili protein subunits were isolated using pili bacteria cutters, and the outer membrane proteins were solubilized and obtained using sodium dodecyl sulfate 0.05% as detergent for Omp isolation. Â The hemagglutination reaction used mice erythrocytes. The cross reactions between subunit pili proteins were conducted by Western blot and Dot blot. Results:. Antibodies against hemagglutinin sub unit pili protein 18 kDa responded to pili protein subunits 18 kDa; 23 kDa; 34 kDa; and 53 kDa; and Omp 23 kDa and 27 kDa. Omp and subunit pili proteins S. flexneri consists of several identical epitopes Â that were responsible for the similarity of the response profile in the cross-reactions of antibodies.Â

PATOMEKANISME PENYAKIT GAGAL JANTUNG KONGESTIF Rachma, Lailia Nur
El-Hayah : Jurnal Biologi Vol 4, No 2 (2014): EL-HAYAH (Vol 4, No 2, Maret 2014)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v4i2.2630

Heart failure is a clinical syndrome characterized by abnormalities in the structure or function of the heart, resulting in inability of heart to pump blood to meet the metabolic needs of the body tissue. Heart failure is characterized by clinical manifestations such as circulation congestion, tightness, fatigue, and weakness. Heart failure is a major problem in industrial and developing Country. Currently, the incidence and prevalence of heart failure tends to increase, it is also accompanied by an increase in mortality of heart failure cases. In the United States, 1 million patients hospitalized due to heart failure cases, which contribute to 50,000 deaths each year. While the number of visits to the hospital due to heart failure estimated at 6.5 million. Heart failure prognosis is generally poor despite the patients accepted adequate therapy. From the data obtained, only about 35% of male patients and 50% female patients who survived after the onset of acute heart failure. Generaly, the data obtained high mortality are occurs in patients with grade IV (presence of symptoms at rest) is about 30-70%, grade III (presence of symptoms with mild activity) 10-20%, class II (presence of symptoms when the activity being 5-10 %). Higher mortality was found in older patients, men, patients with reduced ejection fraction, and in patients with coronary disease. Once someone is suffering from heart failure, then he shall bear the very high cost. In America, the cost of issued for heart failure therapy between 15-40 trillion US$. In this review, we will discuss about pathomechanism of heart failure. So it is expected to be a reference to the diagnosis of patients with heart failure, which is expected to be recognized early on that could ultimately improve the quality of heart failure patient life, and reduce the number of mortality due to heart failure

DAYA ANTIFUNGAL DEKOK KAYU MANIS (Cinnamomum burmanni) TERHADAP Candida Albicans SECARA IN VITRO Rachma, Lailia Nur
El-Hayah : Jurnal Biologi Vol 3, No 1 (2012): EL-Hayah (Vol 3, No 1, September 2012)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v3i1.2218

Candida Â albicans Â is Â the Â most Â oportunisÂ fungi Â that Â cause Â flour Â albus. Â Cinnamomum burmanni have been widely known as therapy for flour albus. This efect caused by its chemical compound such as cinnamaldehyde, eugenol, cinnamic acid, limonene, cathecin, coumarin, linalool, and tannin. This research aims was to know the comparison of antifungal potency of Cinnamomum burmanni on Candida albicans in vitro. The design was true experimental. The decoction concentrations of Cinnamomum burmanni that were used were 8%, 4%, 2%, 1%, 0.5%, 0.25%, and 0.125% with three time repetition. The data was analized with One-way ANOVA test with convidence interval 95% (p 0.05). One-way ANOVA test gave result Cinnamomum burmanni had antifungal potency against Candida albicans. Minimum Inhibitory Concentration (MIC) of Cinnamomum burmanni was 1%. Minimum Fungicidal Concentration (MFC) of Cinnamomum burmanni was 2%. The conclusion was Cinnamomum burmanni had antifungal potency against Candida albicans in vitro. Â Â

Pembentukan dan Pelatihan Kader Posbindu PTM Berbasis Masjid di Kecamatan Turen Kabupaten Malang Tias Pramesti Griana; Lailia Nur Rachma; Herry Darsim Gaffar
JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat) VOL. 5 NOMOR 2 SEPTEMBER 2021 JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat)
Publisher : Lembaga Publikasi Ilmiah dan Penerbitan (LPIP)

Pos Pembinaan Terpadu (Posbindu) Penyakit Tidak Menular (PTM) merupakan bentuk peran serta masyarakat dalam upaya deteksi dini, pemantauan dan pengendalian faktor risiko PTM yang dilaksanakan secara terpadu, rutin, dan periodik serta mandiri dan berkesinambungan. Dewan Masjid Indonsia (DMI) berkeinginan agar semua masjid di kecamatan Turen memiliki Posbindu PTM untuk memelihara kesehatan masyarakat di sekitarnya. Tujuan pengabdian masyarakat ini untuk menginisiasi pembentukan kader Posbindu PTM berbasis masjid di kecamatan Turen dan meningkatkan kualitas kemampuan kader. Digunakan metode Asset Based Community Development, dengan langkah awal dilakukan survey dan Focus Group Discussion dengan pengurus DMI dan takmir masjid. Dilakukan pengamatan pelaksanaan Posbindu PTM, survei tingkat pengetahuan dengan kuesioner dan pelatihan bagi kader dan calon kader. Hasil penilaian kuesioner tingkat pengetahuan didapatkan 73,13% kader Posbindu PTM dapat menjawab 60% soal yang diberikan dengan benar. Pelatihan yang diberikan membantu menambah kemampuan kader dan calon kader Posbindu PTM berbasis masjid di kecamatan Turen Kabupaten Malang.

Cross Reaction among Antibody Pili sub unit Hemagglutinin Proteins and Outer Membrane sub unit Hemagglutinin Proteins of Shigella flexneri Avin Ainur Fitrianingsih; Lailia Nur Rachma; Alvi Milliana; Tinny Endang Hernowati; Aulanni'am Aulanni'am; Sanarto Santoso; Sumarno Reto Prawiro
Journal of Tropical Life Science Vol. 7 No. 1 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.01.01

Shigella Â flexneri is the most common causal agent of shigellosis. Its pili are composed of pili protein subunits. Adhesion molecules can be found on the pili and outer membrane proteins (Omp). A hemagglutination reaction can be used for screening of adhesion molecules. Objectives: The purpose of this study was to determine the molecular weight of the pili protein subunits and outer membrane proteins of S. flexneri that act as hemagglutinin proteins, and to prove whether there is a cross-reaction between antibodies against hemagglutinin pili protein subunits and outer membrane proteins of S. flexneri. Methods: Pili protein subunits were isolated using pili bacteria cutters, and the outer membrane proteins were solubilized and obtained using sodium dodecyl sulfate 0.05% as detergent for Omp isolation. Â The hemagglutination reaction used mice erythrocytes. The cross reactions between subunit pili proteins were conducted by Western blot and Dot blot. Results:. Antibodies against hemagglutinin sub unit pili protein 18 kDa responded to pili protein subunits 18 kDa; 23 kDa; 34 kDa; and 53 kDa; and Omp 23 kDa and 27 kDa. Omp and subunit pili proteins S. flexneri consists of several identical epitopes Â that were responsible for the similarity of the response profile in the cross-reactions of antibodies.Â

NEUTRAL PH AND COLD TEMPERATURES STABILIZE THE PROTECTIVE EFFECT OF CRUDE PILI S. FLEXNERI ON THE ILEAL MUCOSA OF MICE Avin Ainur Fitrianingsih; Lailia Nur Rachma; Alvi Milliana
Jurnal Neutrino:Jurnal Fisika dan Aplikasinya Vol 12, No 1 (2019): October
Publisher : Department of Physics, Maulana Malik Ibrahim State Islamic University of Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/neu.v12i1.15525

Alternative therapies are being sought as a result of the rising concerns with inadequate and untrustworthy medical treatments for Shigella flexneri. The current study aimed to assess the preventive and therapeutic effects of crude pili S. flexneri on of neutral pH and cold temperature against Shigella flexneri infection in immunocompetent mice. Twenty male Swiss albino mice were randomly assigned to one of two groups: control or experimental groups. Each group was subsequently separated into four equal subgroups. In order to infect the mice, 4 weeks before infection, the experimental subgroups were given crude pili S. flexneri orally in every week (three times) until the completion of the research. After following the treatment of crude pili S. flexneri, all group infected with Shigella flexneri. The small intestines of mice were processed and analyzed for the presence of the pathological lesions. Jejunal portions were measured. The findings revealed that vaccinated mice had a statistically significant increase in the quantity of health mucose as compared to non-infected group in ileal sections. Crude pili S. flexneri was administered to the intestinal portions of all subgroups before or after the infection, and the architecture was found to be more or less normal. Our data indicate that crude pili S. flexneri in neutral pH and cold temperature is a useful preventative and a potentially effective treatment medication for S. flexneri infection.

Social Support Build Resilience in Multidrug-Resistant Tuberculosis Patients: A Cross-Sectional Study in MDR-TB Patient Support Group Akbar, Putri Wulan; Rachma, Lailia Nur; Rahmah, Zainabur; Firdaus, Muhammad Iqbal; Nanggali, Bryka Vian
The Avicenna Medical Journal Vol 5, No 2 (2024): The Avicenna Medical Journal
Publisher : Faculty of Medicine, UIN Syarif Hidayatullah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/avicenna.v5i2.42615

Background: Multidrug-resistant tuberculosis (MDR-TB) patient faces multi-dimensional challenges due to the physical factor, long-term treatment, negative stigma, and psychosocial challenge. Addressing these issues patient requires a resilience. The formation of resilience is influenced by both internal and external factors, including social support. This study purposed to assess the social support impact on resilience in MDR-TB patients. Methods: This study was an observational analytic study with cross sectional design. A total of 33 respondents with MDR-TB confirmed, a member of MDR-TB support group, and under treatment were involved. The social support level was measured using the ISEL-12 (Interpersonal Support Evaluation List) questionnaire, while the level of resilience was assessed using the CD-RISC-25 (Connor-Davidson Resilience Scale). Spearman correlation test in SPSS Ver 24.0 was used to examine the relationship between social support and resilience among patients with MDR-TB. Results: Majority of respondents (17 respondents, 51.6%) have good social support and 23 respondents (69.7%) exhibited a good level of resilience. The Spearman correlation test revealed a significant positive correlation between social support and resilience, with a p-value of 0.030 and a moderate strength correlation coefficient (r) of 0.378. Conclusion: This finding showed that good social support toward MDR-TB patients gives positive impact to patients’ resilience. Thus strengthens the system of social support in support group is important to decrease the psychosocial challenges and help to succeed the treatment.

Social Support Build Resilience in Multidrug-Resistant Tuberculosis Patients: A Cross-Sectional Study in MDR-TB Patient Support Group Akbar, Putri Wulan; Rachma, Lailia Nur; Rahmah, Zainabur; Firdaus, Mohammad Iqbal; Nanggali, Bryka Vian
The Avicenna Medical Journal Vol. 5 No. 2 (2024): The Avicenna Medical Journal
Publisher : Faculty of Medicine, UIN Syarif Hidayatullah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/zax66z29

Background: Multidrug-resistant tuberculosis (MDR-TB) patient faces multi-dimensional challenges due to the physical factor, long-term treatment, negative stigma, and psychosocial challenge. Addressing these issues patient requires a resilience. The formation of resilience is influenced by both internal and external factors, including social support. This study purposed to assess the social support impact on resilience in MDR-TB patients. Methods: This study was an observational analytic study with cross sectional design. A total of 33 respondents with MDR-TB confirmed, a member of MDR-TB support group, and under treatment were involved. The social support level was measured using the ISEL-12 (Interpersonal Support Evaluation List) questionnaire, while the level of resilience was assessed using the CD-RISC-25 (Connor-Davidson Resilience Scale). Spearman correlation test in SPSS Ver 24.0 was used to examine the relationship between social support and resilience among patients with MDR-TB. Results: Majority of respondents (17 respondents, 51.6%) have good social support and 23 respondents (69.7%) exhibited a good level of resilience. The Spearman correlation test revealed a significant positive correlation between social support and resilience, with a p-value of 0.030 and a moderate strength correlation coefficient (r) of 0.378. Conclusion: This finding showed that good social support toward MDR-TB patients gives positive impact to patients’ resilience. Thus strengthens the system of social support in support group is important to decrease the psychosocial challenges and help to succeed the treatment.

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