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Harjanto JM
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Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience Nursing

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HUBUNGAN EKSPRESI HSP 70 DAN EKSPRESI RESEPTOR OPIOID MU PADA PENURUNAN NYERI TERAPI BEKAM BASAH Subadi, Imam; Laswati, Hening; JM, Harjanto
Jurnal NERS Vol 11, No 1 (2016): Jurnal Ners
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (456.452 KB) | DOI: 10.20473/%oj.Ners111%y34-39

Abstract

Latar belakang. Terapi bekam basah banyak digunakan untuk mengurangi nyeri,  namun mekanisme penurunan nyeri terapi bekam basah belum jelas. Peregangan dan tusukan pada terapi bekam basah menimbulkan stres pada sel. Sel yang mengalami stres mengekspresikan HSP 70 dan reseptor opioid mu.Tujuan penelitian. Penelitian ini bertujuan membuktikan korelasi  peningkatan ekspresi HSP 70 dan peningkatan reseptor opioid mu pasca terapi bekam basah.Bahan dan Metode. Jenis penelitian ini adalah penelitian eksperimental dengan rancangan randomized control group post test only design. Dua puluh empat tikus jenis Wistar (Rattus norvegicus)  secara random dibagi menjadi tiga kelompok yang masing-masing terdiri dari 8 ekor tikus yaitu kelompok kontrol negatif (tikus normal), kelompok induksi nyeri dengan Complete Freund’s Adjuvant (CFA) saja (kontrol positif) dan kelompok  yang diberi induksi nyeri dan terapi bekam basah (10 tusukan dan tekanan negatif: - 200 mmHg, 5 menit).  Sampel diambil dari kulit dan dilakukan pemeriksaan imunohistokimia dengan antibodi monoklonal anti reseptor opioid mu. Waktu reaksi ambang nyeri diukur memakai hot-plate. Data dianalisa dengan Anova oneway dan Pearson Correlation dengan menggunakan SPSS versi 17.Hasil. Penelitian ini menunjukkan bahwa terdapat peningkatan ekspresi HSP 70 (20,25 ± 3,53; p< 0.05) dibandingkan kelompok kontrol positif (10,50 ± 2,44; p< 0,05) dan peningkatan ekspresi reseptor opioid mu  (21,00 ± 6,34; p< 0.05) dibandingkan kelompok kontrol positif ( 4,25 ± 3,58; p< 0.05). Terdapat peningkatan waktu reaksi ambang nyeri (22,81 ± 6,34; p< 0,05) dibandingkan kontrol positif (11,78 ± 3,56). Terdapat korelasi antara peningkatan ekspresi HSP 70 dan peningkatan reseptor opioid mu  (β= 0.893; p= 0.000) dan ada korelasi antara peningkatan reseptor opioid mu dengan waktu reaksi ambang nyeri (β= 0.713; p= 0.002).Kesimpulan. Terjadi peningkatan ekspresi HSP 70 dan reseptor opioid mu pada terapi bekam basah dan terdapat korelasi antara peningkatan ekspresi HSP 70 terhadap ekspresi reseptor opioid mu.Kata kunci : HSP 70, reseptor opioid mu dan bekam basah 
GLUT 4 DI JARINGAN ADIPOSA (GLUT 4 in Adipose Tissue) Dewi Ratna Sari; Rimbun Rimbun; Tri Hartini Yuliawati; Joni Susanto; Ari Gunawan; Harjanto JM
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 1 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v21i1.1263

Abstract

The decreasing of glucose transporter 4 (GLUT 4) in adipose tissue of diabetic and obesity patients are associated withhyperinsulinaemia and insulin resistance. The adipose tissue can be used as therapeutic targets in the treatment of Diabetes Mellitus(DM). Visceral adipose tissue has different morphology and functional with subcutaneous adipose tissue and Vitamin D has been knownto have contributed in DM. The aim of this study is to know the role of cholecalciferol on the expression of GLUT 4 in subcutaneous andvisceral adiposity of diabetic rats by elucidated in those tissues. The subjects of the study consisted of nineteen male diabetic rats of Wistarstrain, which were divided into control group (K) and three (3) treatment groups (X1, X2 and X3). In order to induce the condition ofDM, the animals were fed with high fat diet for three (3) weeks and administered a single intraperitoneal injection of streptozotocin (35mg/kgBW) at the end of the second week. Cholecalciferol were administered with doses of 6.25 μg/kgBW in X1, 12.5 μg/kgBW in X2and 25 μg/kgBW in X3 per oral everyday within 14 days. The subcutaneous and visceral adipose tissues of the subjects were processedinto histological slides with immunohistochemistry staining. The data were analyzed by one way ANOVA test and paired t-test (α= 0.05,significance p<0.05). In this study was found the significance of GLUT 4 expression in subcutaneous adiposity (p=0.035) is differentbetween the groups, the differences were found between group K and groups X1, X2 and X3. Also there were found the significance differentof GLUT 4 expression in subcutaneous adiposities compared with visceral adiposities in all treatment groups (p>0.05). Based on thisstudy it can be concluded that cholecalciferol could increase the expression of GLUT 4 in the subcutaneous adiposity, but not in visceraladiposity of the diabetic rats.
Co-Authors Ari Gunawan Dewi Ratna Sari Djoni Susanto Hening Laswati Putra Imam Subadi Rimbun Rimbun Tri Hartini Yuliawati