I Ketut Siadi
Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana, Denpasar

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POTENSI PSEUDOMONAS AERUGINOSA SEBAGAI AGENS BIOKONTROL TERHADAP SCLEROTIUM ROLFSII SACC. DAN BIOSTIMULAN PADA TANAMAN KEDELAI KHAMDAN KHALIMI; I GUSTI NGURAH RAKA; I KETUT SIADI
Agrotrop : Journal on Agriculture Science Vol 1 No 2 (2011)
Publisher : Fakultas Pertanian Universitas Udayana

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Potential of Pseudomonas aeruginosa as Biological Control Agents against Sclerotium rolfsii Sacc. and Biological Stimulants on Soybean Plant. The objectives of this experiment were conducted to evaluate the effectiveness ofP. aeruginosa as an agent of biological control and biological stimulants. P. aeruginosawere tested for antagonistic activity against Sclerotium rolfsii Sacc. The test for antagonistic activity by bacteria was done through side by side culture. Soybean seeds that will be used in the rhizobacteria treatment were soaked in rhizobacteria suspension for 15 minutes. For wihout the treatment, seeds soaked with sterile water instead of rhizobacteria suspension. Results of this experiment,P. aeruginosa showed strong inhibitory activity againstS. rolfsii Sacc. on PDA medium. Percentage of inhibitory activity was 94,4%. Application of P. aeruginosa significantly increased the plant growth. The maximum plant hight, the maximum number of leaves, fresh and dry weights of root, fresh and dry weights of shoot, and chlorophyll content on treated plants significantly higher than those of un-treated control plants according to the Duncan’s multiple range test (P<0.05%). These results suggested that application of rhizobacteria could promote the plant growth and increase the yield.
Produksi Benih Cabai Rawit (Capsicum frutescens L.) Bebas TMV(Tobacco mosaic virus) Melalui Dry Heat Treatment I KETUT SIADI; I GUSTI NGURAH RAKA; I GUSTI NGURAH WISNU PURWADI
Agrotrop : Journal on Agriculture Science Vol 2 No 1 (2012)
Publisher : Fakultas Pertanian Universitas Udayana

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The study was done in Denpasar, Bali under greenhouse condition as well as in the field. The objective of the research is to know the effectiveness of dry heat treatment to inactivate TMV which was contaminated chili pepper seeds, and to improves the seeds quality. The seeds which were dry heated under 40ºC for 24 hours and hereinafter 70ºC for 72 hours showed to have no different viability with those of non treated seeds. These indicated that dry heat treatment does not affect to germination of chili pepper seeds. The dry heat treated chili pepper seeds (DHS), non treated ones (NT), and manually TMV-contaminated DHS (DHS+TMV) were planted under field condition. Verification of TMV infection in the 90 days old chili pepper plants by enzyme-linked immunoassay revealed that the rate of TMV infection were 57.89%, 12.5% and 100% for NT, DHS and DHS+TMV. These results indicated that the TMV source was abundance in the field. Infection of TMV in the chili pepper plants given much effect on the plant height, branch number and production. These research results suggest a possibility of using dry heat.