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All Journal Agrotrop : Journal on Agriculture Science International Journal of Biosciences and Biotechnology E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) PASUPATI Indonesian Journal of Fundamental Sciences
I Gusti Ngurah Bagus
Program Studi Agroekoteknologi Fakultas Pertanian Universitas Udayana
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Physics Social Sciences

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UTILIZATION OF RHIZOSPHERE FUNGI TO CONTROL Fusarium oxysporum f.sp. capsici IN VITRO I Made Sudarma; Ni Made Puspawati; Ni Wayan Suniti; I Nyoman Wijaya; I Gusti Ngurah Bagus
International Journal of Biosciences and Biotechnology Vol 2 No 2 (2015)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Fusarium caused wilt disease in chilli pepper and destroyed some farmer crops. Results ofpreliminary research has been discovered that the disease caused by Fusarium oxysporum f.sp. capsici.The alternative environmental friendly method is to find antagonist microbes which is located in therhizosphere of healthy pepper plants. This study aims to find out potentially antagonistic fungi tocontrol Fusarium wilt disease on pepper plants. The fungi were isolated by soil dilution method orviable plate count method on Potato Dextrose Agar medium with antibiotic Livoplaxacin (25%, w/v).Rhizosphere fungi from healthy pepper plants had been identified. A total of 63 spesies belong to 4genera included Penicillium (45 species), Aspergillus (6 species), Trichoderma (9 species) andCandida (3 species). The highest percentage of distribution of rhizosphere fungi are P. digitatum(47.63%), P. expansum (19,05%), T. harzianum (9,53%), A. nidulans, A. niger, Penicillium sp.,Candida albicans, and T. vitrens i.e 4,76% respectively. All of rhizosphere fungi colonies were foundto inhibit Fusarium oxysporum f.sp. capsici in vitro. The best inhibition was found in Aspergillusniger at 88.89 ± 2.2% followed by A. nidulans of 85,56 ± 1,6 %, T. harzianum at 84,45 ± 1,58% , andT. virens by 83,33 ± 1,2%, five days after inoculation. All of them have a very high inhibition criteria.
Pengaruh Penggunaan Mulsa Plastik Terhadap Kelimpahan Serangga Myzus persicae pada Tanaman Cabai Rawit (Capsicum frutescens L.) KADEK DWI UTAMA; I GUSTI NGURAH BAGUS; I KETUT SIADI; DEWA NYOMAN NYANA; GEDE SUASTIKA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.1, Januari 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Effect of Plastic Mulch to Myzuspersicae Insect Abundance in Plant Cayenne Pepper (Capsicum frutescens L.)   Cayenne Pepperis one of theimportantvegetablesgrowncommerciallyinthe tropicsandoccupythe mostextensiveacreageamongother vegetablesin Indonesian. There are fivespeciesarecommonly cultivatedchiliCapsicumannuum(red pepper), C.frutescensL(cayenne pepper), andC.chinensis, C.bacctum, C.pubescens(chili gendot). However,theeconomicpotentialis a typeof chiliC.annuumandC.frutescensL. The purposeofthis study wastoknow theeffect of the useof plasticmulchoninsectabundanceMyzus persicaeoncropsandcayenne pepper. There arethree treatmentsbeing testedareplantingchiliusingblackplasticmulch(MH), a silverplasticmulch(MP) andplantingwithoutmulchorcontrol(K).The results showedthat theuse ofblack plasticmulchandsilverplasticmulchcannotpreventaphids andwhiteflyinchillicrop, however the presence ofblackplasticmulchandsilverplasticmulchcanreduceboththe insectpopulation. Key words :Cayenne Pepper,Myzus persicae, plastic mulch
Keragaman dan Kepadatan Populasi Predator yang Berasosiasi dengan Hama Penting pada Tanaman Kubis (Brassica oleracea L.) NI WAYAN ASRIANI; I GUSTI NGURAH BAGUS; NI NENGAH DARMIATI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.2, No.3, Juli 2013
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT The Predator Diversity and Population Density Associated with Important Pest in Cabbage Plant (Brassica Oleracea L.) The cabbage plant (Brassica oleracea var. capitata L.) is an important vegetable especially in the highland, has been much cultivated and tried by farmers in Indonesia especially in rural areas. The vitamins contained in cabbage that is vitamin A, B and C which have important role in fulfilling human needs. The aim of this research is to find out the diversity and density of predator population associated with important pests in cabbage plant. To find out about the progress of predator population density associated with important pests in cabbage plant. The result of observation to the diversity of predator’s types showed that five types of predators were found associated with important pests in cabbage plant that was Menochilus sexmaculatus, Ischiodon scutellaris, Paederus fuscipes Curt, Dolichoderus bituberculatus, Oecophylla smaragdina. Diversity index of these types of predators was classified as low which was (0.60). Key words: Predator and Cabbage plant.
Aplikasi Ekstrak Bahan Nabati Berbagai Tanaman terhadap Perkembangan Populasi dan Reproduksi Nematoda Puru Akar Meloidogyne spp. pada Tanaman Tomat (Lycopersicum esculentum Mill.) PUTU ANA DIANTARI; MADE SRITAMIN; I GUSTI NGURAH BAGUS
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.2, April 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

ABSTRACT Application of plant extracts to control the population and reproduction of Meloidogyne spp. on tomato (Lycopersicum esculentum Mill.) Root knot nematodes Meloidogyne spp. was one of the pests that can be a limiting in crop cultivation. Efforts to control nematodes in general performed chemically by using synthetic nematicides. Use this Nematisida can negatively impact the environment, especially when the use of nematicides is too excessive. To try to avoid it in a way that is friendly to the environment control using plant-based materials.The purpose of this study was to determine the plant extracts were able to suppress the population of Meloidogyne spp. and determine the most effective plant extracts suppress the population of Meloidogyne spp. the results showed that the nematode population in the soil of 300 g show the lowest was found in the extract treatment plant Piper betle L. is 25 head, with a percentage of 95%, followed by treatment of Carica papaya tail 28.75 (94.25%), Nicotiana tabacum tail 32.25 (93.55%), Allium sativum 49.5 tail (90.1%), Allium sativum 56.5 tail (88.7%), Riccinus tail communis 63.25 (87.35%), Datura stramonium L. 65 individuals (87%), Morinda citrifolia tail 68.28 (86.34%). To the amount of 1 g of root galls in the most tangible effect of treatment indicated by the plant extract of Piper betle L. with the average number of root knot per 1 g of root pieces with a percentage of 96.5 to 17.5%, followed by treatment of the plant Carica papaya extracts 20, 5 pieces (95.9%), Nicotiana tabacum L. 22.5 units (95.5%), Allium sativum fruit 24.5 (95.1%), Capsicum frutescens L. fruit 26.75 (94.65%), Riccinus communis fruit 28.5  (94.3%), Datura stramonium L. 30.5 units (93.9%), Morinda citrifolia L. fruit 32.25 (93.55%). root knot nematode populations in a 1 g root is: Piper betle L. plant extract with an average of 23.75 tails nematode populations with emphasis percentage (95.25%), followed by treatment of Carica papaya 26.0 tail (94.8% ), Nicotiana tabacum L. tail 28.75 (94.25%), Allium sativum tail 30.75 (93.85%), Capsicum frutescens L. 34.0 tail (93.2%), 36.75 Riccinus communis tail (92.65%), Datura stramonium L. tail 42.75 (91.45%), Morinda citrifolia L. 44.0 tail (91.2%). number of egg masses in the 1 g roots of plants treated with each treatment with the control test plant extracts showed Piper betle L. plant extracts resulted in suppression of egg masses is simply the most good 4.0 percentage points to 99.2% suppression, followed by Carica papaya 6.5 points (98.7%), Nicotiana tabacum L. 9.0 points (98.2%), Allium sativum 10.5 points (97.9%), Capsicum frutescens L. 11.75 grains (97, 65%), Riccinus communis 13.25 points (97.35%), Datura stramonium L. 15.0 points (97%), Morinda citrifolia L. 17.25grains(96.5%). Keywords:Caricapapaya,Nicotianatabacum L., Piper betle L.Tomato plants and Meloidogyne spp.
Persebaran, Populasi dan Persentase Serangan Citripestis Sagittiferella Moore (Lepidoptera: Pyralidae) Pada Buah Jeruk Besar di Kabupaten Bangli dan Gianyar KADEK ADI MARTHANA; I GUSTI NGURAH BAGUS; I WAYAN SUSILA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.7, No.4, Oktober 2018
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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The Distribution, Population and Damage Percentage of Citripestis Sagittiferella Moore (Lepidoptera: Pyralidae) on Large Citrus Fruits in Bangli and Gianyar Regencies This research was conducted in Bangli (Kintamani District) and Gianyar Regencies (Sukawati, and Payangan Districts), and in Integrated Pest Management and Disease Laboratory, Faculty of Agriculture, Udayana University, from December 2017 to February 2018. The purpose of this research are to know the distribution, population and demage percentage of citrus borer, Citripestis sagittiferella. This research used a survey method. The research area was divided into three altitudes, Sukawati District (0-450 m asl), Payangan District (altidute> 450-800 m asl), and Kintamani District (altidute>800-1000 m asl). The results showed that C. sagittiferella was found evenly distributed in all altitude locations, in Sukawati District, Payangan District and Kintamani District with total population of 3684 larvae, 2152 larvae and 916 larvae respectively. The average larva population found in citrus fruits damage was 6.28 larvae per fruit. Percentage of damage in Kintamani, Payangan, and Sukawati Districts were 30.37%, 25.26%, and 18.18%, respectively. The correlation test results show a very strong correlation between the larval population and the percentage of damage with r = 0.988.
Deteksi Keberadaan Penyebab Penyakit Citrus Vein Phloem Degeneration (CVPD) secara Molekuler pada Tanaman Jeruk Siam (Citrus nobilis Lour var. microcarpa Hassk) berdasarkan Variasi Gejala Klorosis IKA NURHAYATI; WAYAN ADIARTAYASA; I GUSTI NGURAH BAGUS
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.5, No.4, Oktober 2016
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Detection of the Presence of Citrus Vein Phloem Degeneration (CVPD) Disease Causing in Molecular on Citrus (Citrus nobilis Lour var. microcarpa Hassk)which Based on Variation of Chlorosis Symptoms This research was aimed to identify the presence of Liberobacter asiaticum which caused of Citrus Vein Phloem Degeneration disease on citrus leave with different chlorosis symptoms. This research was conducted at Laboratory of Genetics Resources and Biology Molecular, Udayana University. The leaves sample were measured the chlorophyll content using a chlorophyll meter and then were identified in molecular using Polymerase Chain Reaction (PCR) technique, using primer O11 and O12 which will amplified the specific fragmen of 16S rDNA. The result for chlorophyll content indicated that the leaf samples were containing 26.80, 36.52, 36.62, 40.74, 44.56, 47,06, 58.44 and 65.16 SPAD.DNA amplification showed that the leaves which contained chlorophyll at 58.44 and 65.16 SPAD didn’t show DNA bands with size 1160 bp, and leaves which contained chlorophyll at26.80, 36.52, 36.62, 40.74, 44.56 and 47.06 SPAD showed DNA bands 1160 bp. Therefor the DNA bands 1160 bp is expression by L. asiaticum, then the citrus leaf samples were detected positive for the L. asiaticum and the samples were positive infected by CVPD disease, so that the chlorophyll content of citrus leaves at ?47.04 SPAD can be used as basis to diagnosis CVPD disease on citrus plants.
Pengaruh Media dan Umur Biakan Jamur Metarhizium anisopliae M. terhadap Tingkat Kematian Larva Oryctes rhinoceros L. (Scarabaeidae ; Coleoptera) NI MADE WINDA UTARI; PUTU SUDIARTA; I GUSTI NGURAH BAGUS
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.2, April 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRAK Influence of Media and Fungus Metarhizium anisopliae M. Age culture to Larvae Mortality of Oryctes rhinoceros L. (Scarabaeidae; Coleoptera) Oryctes rhinoceros L. is importance pest that can be serious problem to coconut plantations. The utilization of fungus Metarhizium anisopliae M. as biological agents has became a recommended alternative choice considering its selective and safe infect to ecosystem. The purposes of this study in order to find out the effectiveness rates of media and culture age of fungus M. anisopliae on   O. rhinoceros larvae mortality and to find out the culture age of M. anisopliae in corn and rice media that cause O. rhinoceros larvae mortality. This study used in vitro and direct trial method. The result showing that fungus Metarhizium anisopliae in culture age of 4 weeks have ability to infect the larvae since 6th and on  14th days after application the all of  larvae was infected. On the other hand the fungus in culture age of 3 weeks the  larvae was infected since 7th day after application and on 21st days after application the all larvae was infected. Keywords:, Oryctes rhinoceros L. Metarhizium anisopliae M.
Populasi dan Serangan Hama Polong Kedelai Etiella zinckenella (Treitschke) (Lepidoptera: Pyralidae) yang Diperlakukan dengan Insektisida Berbahan Aktif Klorpirifos 500 g/l dan Sipermetrin 50 g/l NGAKAN PUTU MEIKA RUSYANA; I GUSTI NGURAH BAGUS; ANAK AGUNG AYU AGUNG SRI SUNARI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.7, No.2, April 2018
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Population and Attack of Soybean pods Etiella zinckenella (Treitschke) (Lepidoptera: Pyralidae) treated Insecticide with Activity Klorpirifos 500 g/l and Sipermetrin 50 g/l The study was conducted to find out the Population and Attack of Soybean pods, Etiella zinckenella (Treitschke) (Lepidoptera: Pyralidae) treated Insecticide with Activity Klorpirifos 500 g/l and Sipermetrin 50 g/l. This research was conducted in Gunaksa Village, Dawan District, Klungkung Regency, from December 2016 until February 2017. The purpose of this research is to know the effect of insecticide active ingredient (Klorpirifos 500 g/l and Sipermetrin 50 g/l) to soybean pest the populations of and the rate of E. zinckenella pests on soybean crops. This study used a randomized block design (RBD), with five treatment levels (concentration) and five replications. The results showed that insecticide with chlorpyrifine 500 g/l and sipermetrin 50 g/l at concentration 1.50 ml/l were able to suppress E. zinckenella and increase production.
Keragaman dan Daya Hambat Spora Tular Udara yang Mengkontaminasi Media Baglog Jamur Tiram (Pleurotus ostreatus (Jacq. ex Rr) Kummer) I MADE SUDARMA; NI MADE PUSPAWATI; NI NENGAH DARMIATI; KETUT AYU YULIADHI; NI WAYAN SUNITI; I GUSTI NGURAH BAGUS; I NYOMAN WIJAYA; DWI WIDANINGSIH
Agrotrop : Journal on Agriculture Science Vol 5 No 2 (2015)
Publisher : Fakultas Pertanian Universitas Udayana

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Diveristy and Inhibition of Air-Borne Spores That Contaminate Substrat of OysterMushroom (Pleurotus ostreatus (Jacq. Ex Fr) Kummer). The aim of research to know thediversity, prevalence and inhibition of air-borne spores that could potentially contaminatesubtsrat baglog of oyster mushrooms. In this study using the method: (1) the arrest of sporescarried out during the hours of 7 am to 13 pm by placing three petri dish that already containsmedia PDA 3 pieces, and repeated 3 times, (2) inhibition ability of air-borne spores againstoyster mushroom. The place and time of the study conducted at Jl. Siulan Gang Zella No. 7Denpasar, and implemented in October 2014 to February 2015. The results showed that 13genera, including Aspergillus spp, as many as 10 with a prevalence of 13.51%, Aspergillusniger 7 (9.45%), Brachysporium sp., 1 (1.35%) , Cunninghamella sp. 1 (1.35 %), Fusariumspp. as many as 19 (25.6 %), Giotrichum sp. 1 (1.35%), Mucor spp. , a total of 18 (24.32%),Neurospora spp., as many as 8 (10.81%), Penicillium spp. 2 (2.70%), Phytophthora spp. 4(5.40%), Stachybotrys sp. 1 (1.35%), Trichoderma sp. 1 (1.35%) and Umbelopsis sp. 1 (1.35%). The highest prevalence achieved by Fusarium spp . amounting to 25.67 %, followed byMucor spp. 24.32%. Diversity index (H') was obtained from each replicate of 0.6438, 0.7048,0.5611 with a 0.6366 average. Dominance index (C) obtained by 0.6331. 0.8646, 0.6146, andaverage 0.7041. Diversity including < 1, marked by low diversity and dominance index closeto 1, meaning there is dominance. The dominance held by Fusarium spp . and Mucor spp.Inhibitory effects of air -borne fungi on the growth Oyster mushroom in vitro; The highestachieved by Fusarium spp, with inhibition of 94.00 ± 1.2%, followed by Aspergillus spp.amounting to 92.15 ± 1.5%, and the lowest was achieved by Penicillium spp . amounting to70.37 ± 2.5%.
Status Penyakit Layu pada Tanaman Cabai Rawit (Capsicum Frutescens L.) di Banjarangkan, Klungkung I MADE SUDARMA; NI MADE PUSPAWATI; NI WAYAN SUNITI; I GUSTI NGURAH BAGUS
Agrotrop : Journal on Agriculture Science Vol 4 No 2 (2014)
Publisher : Fakultas Pertanian Universitas Udayana

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Status of wilt disease in pepper (Capsicum frutescens L.) at Banjarangkan, Klungkung. Wilt disease in pepper , has led to a total yield loss in Banjarangkan, Klungkung regency. Until now the disease was still a threat to farmers in the area pepper. The purpose of research to study the disease includes symptoms, causes disease, the percentage of the disease and the rate of infection (r). Research using existing observations of the disease in three plots of local farmers. Each plot the observed number of diseased plants and entire plants. Samples of diseased plants put in a plastic bag, then placed in an ice box , to be observed macroscopically in the laboratory. The study was conducted in two places, namely surveys Banjarangkan disease in Klungkung , and isolation of the pathogen as well as pathogenicity test carried out in the Laboratory of Plant Pathology, Faculty of Agriculture, Udayana University, Jl. PB Sudirman Denpasar-Bali. The research was conducted from June to November 2013. The results showed that the pathogen that causes wilt disease in pepper at Banjarangkan, Klungkung regency was the fungus Fusarium oxysporum f.sp. capsici, with the disease incidence was 76.67%. Fusarium wilt disease epidemic in pepper indicated by the rate of infection, first gained 0.44 per unit per day, then decreases with time, 0.23, 0.12 and 0.11 per unit per day respectively. The rate of infection ranged from 0.11 to 0.44 per unit per day, this means that the moderate criteria.
Co-Authors Abd. Rasyid Syamsuri Anak Agung Ayu Agung Sri Sunari DWI WIDANINGSIH GEDE SUASTIKA GEDE WIJANA I Dewa Nyoman Nyana I Ketut Siadi I MADE SUDARMA I MADE SUDARMA I MADE SUDARMA I MADE SUDARMA I NYOMAN WIDNYANA PUTRA I NYOMAN WIJAYA I Putu Sudiarta I Wayan Susila Ida bagus Nyoman Putra IKA NURHAYATI KADEK ADI MARTHANA KADEK DWI UTAMA KETUT AYU YULIADHI MADE SRITAMIN NGAKAN PUTU MEIKA RUSYANA Ni Made Gitari Ni Made Puspawati NI MADE WINDA UTARI NI NENGAH DARMIATI NI WAYAN ASRIANI NI WAYAN SUNITI PUTU ANA DIANTARI WAYAN ADIARTAYASA