WAYAN ADIARTAYASA
Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ISOLATION AND IDENTIFICATION OF ARBUSCULAR MYCORRHIZAL FUNGI (AMF) IN CASHEW PLANTS (Anacardium occindentale L.) IN DATAH VILLAGE, ABANG DISTRICT OF KARANGASEM REGENCY I Ketut Suada; Evy Prima; Made Sritamin; I Wayan Adiartayasa; I Gede Ketut Susrama; I Gede Putu Wirawan
International Journal of Biosciences and Biotechnology Vol 5 No 2 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (578.137 KB) | DOI: 10.24843/IJBB.2018.v05.i02.p10

Abstract

Isolation and identification of arbuscular mycorrhizal fungi (AMF) in cashew plants was carried out in Datah Village, Abang District of Karangasem Regency. This study aims to determine the diversity of mycorrhizal spores in the cashew rhizosphere and AMF infections in the roots of cashew plants. The result in microscopic observations found 5 genera of AMF spores such as Glomus, Racocetra, Entrophospora, Septoglomus, and Acaulospora and the population of the genus Glomus were found most dominant in the rhizosphere of cashew plants. The result of staining root with trypan blue obtained the internal structure of AMF in the form of vesicles, arbuscules and hyphae found in root tissue of cashew plants. The result of AMF DNA amplification with primer NS31 and AML2 showed 560 bp bands in electrophoresis with 1% agarose gel determined AMF infection in root of cashew plants.
THE MOLECULAR DETECTION OF CITRUS VEIN PHLOEM DEGENERATION (CVPD) PATHOGEN (Liberobacter asiaticus) IN Diaphorina citri KUWAYAMA (HOMOPTERA : PSYLLIDAE) AND OTHER INSECTS ASSOCIATED WITH CITRUS PLANT I Nyoman Wijaya; Wayan Adiartayasa; Made Sritamin
International Journal of Biosciences and Biotechnology Vol 5 No 2 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (147.785 KB) | DOI: 10.24843/IJBB.2018.v05.i02.p07

Abstract

The research was coducted at Laboratory of Biotechnology Faculty of Agriculture Udayana University. The purpose of this research were to investigate moleculer detection of CVPD pathogen in its vector, other phytophagous insects on citrus cv, Siam and kemuning (Murraya paniculata L. Jack.). The result of this research showed that adults and nymphs of Diaphorina citri Kuw. could transmit CVPD disease, but the pathogen of CVPD could not be transovarially transmited. As a host of CVPD disease , kemuning could be source of infection. The adult of Toxoptera citricidus Kirkaldy and Maleuterpes dentipes Hell. could not potensial transmited CVPD disease.
ANTIOXIDANT ACTIVITY OF THE TOPICAL PREPARATION OF BULUNG SANGU (Gracilaria spp) EXTRACT Maria Malida Vernandes Sasadara; I Gede Putu Wirawan; Made Sritamin; I Ketut Suada; Wayan Adiartayasa
International Journal of Biosciences and Biotechnology Vol 7 No 2 (2020)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (194.155 KB) | DOI: 10.24843/IJBB.2020.v07.i02.p04

Abstract

Bulung Sangu (Gracilaria spp) as many other macroalgae, is a source of beneficial phytochemical for health. Phytochemicals in macroalgae are widely used as active ingredients in cosmetic preparations. The study was aimed to determine the antioxidant properties of Bulung Sangu formulated in topical preparation. Bulung Sangu was macerated in ethanol 96% following by extract formulation in cream preparation in two concentrations: 5% and 10%. Antioxidant activity was determined against DPPH radical, compared to ascorbic acid. Percentage of reducing power in five concentration series (10, 20, 30, 40, and 50 µm/ml) statistical analyzes using one-way ANOVA with 95% of confidence level, following by IC50 measurement. Absorbance was read in wavelength of 517 nm. Concentration of 5% and 10% cream extract exhibited IC50 of 13,091 µg/ml and 11,867 µg/ml, respectively. These values are lower than IC50 of ascorbic acid (18,505 µg/ml), indicating the strong antioxidant properties of Bulung Sangu extract cream which is more potential than ascorbic acid. Bulung Sangu exhibit a potential source for being developed as medicine and cosmetics in topical preparation.
PHYTOCHEMICAL ANALYSIS AND ANTIOXIDANT ACTIVITY OF PURNAJIWA FRUIT EXTRACT (Euchresta horsfieldii (Lesch.) Benn.) Romulia Trielva Apriliani; I Gede Putu Wirawan; Wayan Adiartayasa
International Journal of Biosciences and Biotechnology Vol 8 No 1 (2020): Vol. 8 No. 1, September 2020
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2020.v08.i01.p04

Abstract

Antioxidant is a chemical compound that could give one or more electrons to inhibit the free radical reaction. One of the plants that potentially produce antioxidants is purnajiwa (Euchresta horsfieldii (Lesch.) Benn.). The research was conducted using the fruit of purnajiwa. The purpose of this study is to know antioxidant activity from purnajiwa fruit using the DPPH method and measured by UV-Vis spectrophotometry. The extract of purnajiwa fruit gained by the maceration method using 96% ethanol solvent. The results of phytochemistry screening found out that the extract contains flavonoid, alkaloid, saponin, tannin, and terpenoid. According to flavonoid testing using quercetin, the results obtained from purnajiwa extract contained flavonoid of 1,651 mg QE/g. The activity test of antioxidants quantitatively by DPPH using UV-Vis spectrophotometry found out that purnajiwa extract has IC50 value of 57,28 ppm so that purnajiwa extract has strong antioxidant activity that could be used as natural antioxidant resources.
INCREASING LOCAL FRUITS COMPETITIVENESS IN ENTERING THE TOURISM MARKET IN BALI I Gede Putu Wirawan; Ketut Srie Marhaeni Julyasih; Wayan Adiartayasa; I Nyoman Wijaya; I Putu Anom
International Journal of Biosciences and Biotechnology Vol 2 No 1 (2014)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

The study focused on improving the fruit quality supported by handling of the cultivation, harvest and post-­??harve st handling such as fruit packing, transportation, storage, and others, as well as marketing strategiesand regulations that are ne eded locally, so local Bali fruits can compete better in the Bali tourism market.The purposes of this study were to find some problems face by the local fruits to e nter the tourism marke t inBali. The local fruits studied were citrus, salacca, mangoes, mangosteen, banana, and papaya. This study wasthrough multiple surveys such as, inte rviews with farme rs, whole salers, fruit suppliers, and the manage rsof the hote ls and restaurants. The results of this study found some local fruits have e nte red the tourismmarket in Bali, in addition, local people in Bali te nd to consume the imported fruits for some culturalceremony, events and others.The results of this study showthat the main problems in the local fruits suchas citrus and banana were harvesting, packaging, storage, and transportation. The laboratory studies foundthat some microbes and inse cts infe cted local fruits due to the lackof handling such as packaging, storage,and transportation. The findings of this study was local fruits such as orange s and bananas face manyobstacles ranging from harvest, post-­??harvest management, transportation, and the marketing strategies.Many problem has found on bananas such as damaged on storage, as well as citrus fruits. Improvements tothe appearance of the fruit has been tried to do by the application of emulsion of he rbal oils, oleic acid, wax,twee n, and the results showe d the fruits still in a fre sh condition afte r four weeks of treatme nt while theuntre ated fruits were rotten due tothe infe ction of microbes. The blackspots of insects ormicrobes infectionson the treated fruits can be eliminated as well. Some inse cts and microbial attacks were found that leads tofruit rot which can essentially be removed by tre atment with antimicrobial tre atment. This research tries tochart the effects on the competitiveness of local fruits, such as continuity of production, standardization andregulation which could be developed at the local level in the village community.
PELATIHAN PENGENDALIAN PENYAKIT LAYU PADA TANAMAN JAHE DI DESA TARO KABUPATEN GIANYAR I N. Wijaya; W. Adiartayasa; M. Sritamin; I G. Ngurah Bagus
Buletin Udayana Mengabdi Vol 12 No 2 (2013): Volume 12 No.2 – September 2013
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Training on ginger wilt diseases was held at Taro village, Gianyar regency on 18 st July 2013. The activitiesaimed how to know control of ginger wilt diseases. The training was attended by 25 participants from local farmergroups of Taro village. The methods used in activities were lectures, demonstration and practice in the gingerfield. All participants participated enthusiastically and hope they have the next intensive training again.
PENDIDIKAN DAN PELATIHAN PENGENDALIAN KUTU LONCAT JERUK (Diaphorina citri Kuwayama) SEBAGAI HAMA DAN VEKTOR PENYAKIT CVPD DI DESA TARO, GIANYAR Wijaya I N.; M. Sritamin; M. Mega Adnyana; W. Adiartayasa; I G. N. Bagus
Buletin Udayana Mengabdi Vol 11 No 2 (2012): Volume 11 No.2 – September 2012
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

Training on jumping plantlice of citrus (Diaphorina citri Kuw) the vector of CVPD (Citrus Vein Phloem Degeneration) has been held at Taro village, Gianyar regency on 30st July 2012. The activities aimed to know bioecology of D. citri, the vector of CVPD. The training was attended by 32 participants from local farmer groups of Taro village. The method used in activities were lectures, demontration and practical in the citrus field. All participants participited anthusiastically and hope the have the next intensive traning of the citrus culture.
AWAS BAHAYA SERANGAN LALAT BUAH PADA TANAMAN JERUK I.N. Wijaya; W. Adiartayasa
Buletin Udayana Mengabdi Vol 17 No 3 (2018): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (442.253 KB) | DOI: 10.24843/BUM.2018.v17.i03.p05

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Sebagian besar penduduk Desa Katung, Kecamatan Kintamani, Kabupaten Bangli sebagai petani jeruk yang tergabung dalam kelompok Subak Abian Kerta Winangun yang beranggotakan 97 orang. Pertanaman jeruk di daerah ini sangat berhasil, sehingga pendapatannya meningkat. Namun belakangan, petani resah karena tanaman jeruknya terserang hama lalat buah. Oleh karena itu perlu diadakan penyuluhan dan pelatihan tentang pengelolaan hama lalat buah pada tanaman jeruk. Adapun tujuan penyuluhan dan pelatihan ini untuk memberikan pengetahuan dan keterampilan kepada para petani setempat tentang bahaya serangan hama lalat buah serta pengendaliannya pada tanaman jeruk meliputi : gejala serangan, faktor-faktor yang mempengaruhi perkembangan lalat buah dan cara pengendaliannya. Metode yang digunakan dalam pelatihan ini adalah penyampaian materi di ruangan dan praktek di perkebunan jeruk. Kegiatan penyuluhan dan demontrasi dilaksanakan hari Sabtu, tanggal 12 Agustus 2017 mulai pukul 10.00 sampai 14.00 WITA, bertempat di Balai Banjar Katung, Kecamatan Kintamani, Kabupaten Bangli. Pelatihan ini dipandu oleh 4 (empat) orang pelatih yang berasal dari Program Studi Agroekoteknologi Fakultas Pertanian Universitas Udayana. Pelatihan diikuti diikuti oleh 69 orang peserta. Penceramah memaparkan materinya dengan menggunakan alat peraga berupa power point dan menggunakan spesimen berupa gejala serangan lalat buah. Kemudian dilanjutkan dengan praktek lapangan selama 60 menit. Dalam pelaksanaan pelatihan tersebut, petani sangat antusias untuk mengikuti penyuluhan. Hal ini terbukti dari banyaknya pertanyaan-pertanyaan yang diajukan. Untuk mengetahui keberhasilan kegiatan ini, maka dilaksanakan evaluasi dengan pengisian kuisioner sebelum dan sesudah pelatihan.
PENDIDIKAN DAN PELATIHAN PENGENDALIAN TERPADU PENYAKIT CVPD (CITRUS VEIN PHLOEM DEGENERATION) PADA PERTANAMAN JERUK DI DESA PELAGA, KECAMATAN PETANG, KABUPATEN BADUNG WAYAN ADIARTAYASA; KETUT SIMPATI; KETUT SUMIARTHA
Buletin Udayana Mengabdi Vol 5 No 1 (2006): Volume 5 No.1 – April 2006
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

ABSTRACT Education and training on integrated pest management (IPM) of CVPD (Citrus Vein Phloem Degeneration) was held at Pelaga village, Badung regency on 22 September 2005. The activities aimed to improve knowledge of the farmers about CVPD and factors influencing the intensity, insect vector and integrated pest management of CVPD. The topics convered were disease symptons, and pathogen bacteria, Diaphorina citri are as vector insect infection mechanism, disease distribubution and integrated management of CVPD. The training was attended by 26 participants from local groups of Pelaga village. The method used in activities were lectures, demonstration and practical work in the citrus field. All participants enthusiastically took part and hoped to have the next intensive traning of citrus culture.
PENDIDIKAN DAN PELATIHAN PEMBIBITAN JERUK BEBAS PENYAKIT CVPD (Citrus Vein Phloem Degeneration) DI DESA KATUNG KINTAMANI Adiartayasa W; N.N. Darmiati; I K. Simpati
Buletin Udayana Mengabdi Vol 10 No 1 (2011): Volume 10 No.1 – April 2011
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

The community service in the form of training about free orange seed of disease CVPD (Citrus Vein Phloem Degenaration) was conducted in Katung village, district of Kintamani, Bangli regency on 10 August 2007. The training activity aimed to improve knowledge of farmers about CVPD disease, symptom, transmission, insect vector, and integrated pest management. The topics covered were disease symptoms, and pathogen bacteria, Diaphorina citri are as insect vector infection mechanism, election of free orange mains crops CVPD, disease distribution and integrated management of CVPD. The training was attended by 25 participants from local groups of Katung village. The methods used in this activity were lectures, demonstration and practical work in the citrus field. All participants enthusiastically took part and hoped to have the next intensive training of citrus culture.