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All Journal Journal of Food and Pharmaceutical Science Jurnal Sain Veteriner Jurnal Veteriner Jurnal Kedokteran Hewan Journal of Food and Pharmaceutical Sciences

Pudji Astuti

Departemen Fisiologi, Fakultas Kedokteran Hewan, Universitas Gadjah Mada. Jl. Fauna 2, Kampus UGM, Karangmalang, Yogyakarta, Indonesia 55281

Author-ID : 2938188

Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Veterinary

Published : 8 Documents Claim Missing Document

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Articles

Title

The Heart Activities in Some Environmental Temperature on the Rats (Rattus norvegicus) Amelia Hana; Pudji Astuti; Yuda Heru Fibrianto; Sarmin Sarmin; Claude Mona Airin
Jurnal Sain Veteriner Vol 30, No 2 (2012): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The aim of this research was to know the heart activity of rats (Rattus norvegicus) at some environmental temperature. Ten rats (Rattus norvegicus), male, 2 months old, the average weight of 200 gr purchased at experiment Animal Care Unit Gadjah Mada University, Yogyakarta. Allf rats were adapted in a cage with food and water ad libitum. Furthermore, each tail rat was measured heart activity is heart rate frequences (beats per minute), mean blood pressure (mmHg), systolic blood pressure (mmHg) and diastolic blood pressure (mmHg) at the environmental temperature of 30o C, 32o C, 34o C, 36o C, 37o C, 38o C, and 40o C measured with a blood pressure cuff BP-98A at coccygea vein for 3 minutes at each temperature. The measurement was repeated 3 times. The data of the heart activities obtained were analyzed by using one way analysis of variance. It was concluded that the environmental temperature of 30o C was optimal to the heart activities of the rats, including heart rate frequences , blood pressure, systolic and diastolic blood pressures.Keywords: heart rate frequences, blood pressure, systolic and diastolic, environment temperature, Rattus norvegicus

Hiperglikemia Tidak Menyebabkan Perubahan Bobot Badan dan Jumlah Neuron Mienterik Nitrergik Usus Halus Tikus Wistar (Rattus norvegicus) Amelia Hana; Pudji Astuti; Yuda Heru Fibrianto; Sarmin Sarmin; Claude Mona Airin; Tauhid Tauhid
Jurnal Sain Veteriner Vol 36, No 2 (2018): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Digestive dysfunction is a common symptom of diabetes. Diabetes can lead to the loss of enteric neurons as well as nerve dysfunction and gastrointestinal complications. There has been no complete report of mienteric nitrergic of small intestines on hyperglycaemia condition, so the purpose of this study was to assess changes of nitrergic neural profile of small intestines on hyperglycemic Wistar strain rats (Rattus norvegicus). Twelve rats were adapted for 3 days, then they were randomly divided into 3 groups namely group I (K-1, as control), group II (K-2), and group III (K-3). Before treatment all rats were fasted for 12 hours, rats K-1 were induced with 1.0 ml of buffer citrate single peritoneal dose; rats K-2 were induced with 40 mg streptozotocin (STZ)/ kg body weight of single peritoneal dose; and rats K-3 were induced with 45 mg STZ /kg body weight of single peritoneal dose. On the 1st and 5th days after STZ induction, blood was collected through the coccigea vein to measure blood glucose levels and weigh the weight. On the day 5th the rats were uthanated with a high dose of ketamine, dissected and taken small intestines (duodenum, jejunum, and ileum) for staining with Nicotinamide Adenine Dinucleotide Phosphate-diaphorase (NADPH-d). Morphometric changes of nitrergic neurons of the small intestine segments were observed. The results showed that on the 5th day increased blood glucose levels of the K-2 and K-3 groups were significantly higher than K-1 (P <0.01). The weight of three groups did not show any significant improvement difference (P> 0.05). The number of neurons in the duodenum, jejunum and ileum of K-2 group increased higher than that of K-1 and K-3 groups. These results indicate that in hyperglycaemic states, the number of nitrergic neurons increases (K-2 group), and there is a significant decrease in the number of neurons (group K-3) compared with K-1 and K-2 groups (P <0.01). The results of this study concluded, that the STZ induction treatment causes hyperglycemia. Hyperglycemia does not affect to the body weight and the number of nitrergic neurons of small intestines in Wistar strain rats (Rattus norvegicus).

Gonadotropine Releasing Hormone (GnRH) Receptor Profile from Cow’s Hypothalamus Irma Dian Nurani; Claude Mona Airin; Pudji Astuti; Khrisdiana Putri; Bambang Sutrisno
Jurnal Sain Veteriner Vol 38, No 2 (2020): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.51882

Gonadotropine Releasing Hormone (GnRH) is one of the recommended hormones to overcome ovulation problems and it can increase pregnancy rate so that it is used in government programs to increase cattle population in Indonesia, although the results are not yet optimal. The purpose of this study is to compare the composition of bovine GnRH receptor nucleotides with the GenBank reference so that the level of genetic diversity of bovine receptors in Indonesia is known. PCR product sequencing using Promoter F and Exon 1 R primers were further aligned with the reference sequences of Bos Taurus GnRHR mRNA GenBank using the MEGA X program. The results of the analysis found the presence of Single Nucleotide Polymorphism (SNP) in the coding region of 1st cow position 38(A> T), 261(C > T), 342(C >T), 411(C >T) and 495(C > T) and 2nd cow positions 261(C >T). Changes in amino acids were also detected in 1st cow position 13 (H> L). The presence of SNP was found to indicate genomic variation between individuals at cattle receptors in Indonesia.

Kadar Hormon Prolaktin Pada Kambing Peranakan Ettawa,Sapera dan Saanen Pada Berbagai Status Fisiologi Sarmin Sarmin; Irkham Widiyono; Devita Anggraeni; Claude Mona Airin; Pudji Astuti
Jurnal Sain Veteriner Vol 40, No 3 (2022): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.76207

This study examines the prolactin hormone profile in Etaawa, Sapera and Saanen lactating goats, adult females, pregnant does, and yearlings. This study used a total of 80 randomly selected goats with details of 26 Ettawa Crossbreed goats (eight lactating, six adult females, six pregnant, and six heifers), 32 Sapera goats (eight lactating, six adult females, twelve pregnant, and six yearlings) and 22 Saanen goats (five lactating, four adult females, six pregnant and yearling). All goats were declared clinically healthy. Blood sampling was performed through the jugular vein in each animal; then, serum was analyzed with an Enzyme-linked immunosorbent assay (ELISA) to prolactin concentration. High prolactin concentrations were found in Saanen goats in pregnant physiological status compared to Sapera and Ettawa crossbreed goats.

Co-Authors Amelia Hana Amelia Hana Amelia Hana Amir Husni Bambang Sutrisno Claude Mona Airin Devita Anggraeni HERA MAHESHWARI Irkham Widiyono Irma Dian Nurani Khrisdiana Putri LUTHFIRALDA SJAHFIRDI Putri Krida Gita P. Raden Rara Amelia Hana Rusyda Nurshitaningrum Sarmin Sarmin Tauhid Tauhid Yuda Heru Fibrianto Yuda Heru Fibrianto

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