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All Journal HAYATI Journal of Biosciences MEDIA KONSERVASI Jurnal Veteriner Jurnal Ilmiah Pendidikan Pancasila dan Kewarganegaraan Jurnal Penelitian Pendidikan IPA (JPPIPA) AL-HAYAT: Journal of Biology and Applied Biology JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI Jurnal Kedokteran Hewan

LUTHFIRALDA SJAHFIRDI

Departemen Biologi Fakultas Matematika Dan Ilmu Pengetahuan Alam Universitas Indonesia, Jakarta

Author-ID : 383178

Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Education Electrical & Electronics Engineering Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health Social Sciences Veterinary

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Diurnal Patterns of Testosterone and Cortisol Metabolites in Fecal of Javan Gibbons (Hylobates moloch) in Captivity PUDJI ASTUTI; TUTY LASWARDI YUSUF; ERIC HAYES; HERA MAHESHWARI; LUTHFIRALDA SJAHFIRDI; DONDIN SAJUTHI
HAYATI Journal of Biosciences Vol. 13 No. 2 (2006): June 2006
Publisher : Bogor Agricultural University, Indonesia

The aims of this research were to determine diurnal patterns of testosterone and cortisol metabolites to predict the testis functional status. In this study, fecal testosterone and cortisol were quantified in 77 samples from three male Hylobates moloch during a course of three months period. These data showed that the highest concentration of fecal testosterone occured at 18.00-06.00 (23.61 ng/g dried feces), then declined gradually. The lowest concentration was in the evening (5.54 ng/g dried feces). Our tests showed that there was a decrease in the mean testosterone concentration from 06.00-10.00 to 10.00-14.00 to 14.00-18.00. For cortisol, the highest concentration occured at 06.00-10.00 (597.84 ng/g dried feces), then decline gradually in the evening (225.73 ng/g dried feces). Key words: Hylobates moloch, feces, testosterone, cortisol, diurnal pattern

Fecal Steroid Profile of Female Javan Gibbons (Hylobates moloch) Maintained in Pairing-Typed Cage Hera Maheshwari; Luthfiralda Sjahfirdi; Pudji Astuti; Bambang Purwantara; Hadi Sukardi Alikodra; Dondin Sajuthi; Reviany Widjajakusuma
HAYATI Journal of Biosciences Vol. 17 No. 1 (2010): March 2010
Publisher : Bogor Agricultural University, Indonesia

Estrone conjugate (E1C) and pregnanediol glucuronide (PdG) were predominant steroid metabolites of estrogen and progesterone in feces of most primates and could be used to evaluate ovarian function. These metabolites were determined along with records of genital swelling throughout 3-4 months period from three female Javan Gibbons (Hylobates moloch) maintained in pairing-typed cage at Schmutzer Primate Center, Jakarta (Ullah) and at Taman Margasatwa Taman Sari, Bandung (Donna and Citah). Following methanolic extraction of lyophilized fecal powder, samples were analyzed using enzyme linked immunosorbent assay (ELISA) for E1C and PdG. In all of the three females observed, both hormone profiles did not indicate any regular cycle of ovarian function even though genital swellings were sometimes observed. In one female (Donna) the hormone patterns showed clear signs of cycle irregularities with extended luteal phase of 40 days and erratic pattern of follicular phase. Of the other two females, no ovarian cycle was found. The data indicate that the fecal steroids analysis is a practical and valuable diagnostic tool for providing reliable information on ovarian function in Javan Gibbon. Factors affected reproductive hormonal profile should be taken in consideration in trying to achieve success in captive breeding program for this species.

FECAL STEROID PROFILE AND GENITAL SWELLING OF FEMALE JAVAN GIBBONS (Hylobates moloch Audebert 1797) MAINTAINED IN INDIVIDUAL CAGE Hera Maheshwari; Luthfiralda Sjahfirdi; Pudji Astuti; Bambang Purwantara; Hadi Sukadi Alikodra; Dondin Sajuthi; Reviany Widjajakusuma; Mozes R. Tolihere
Media Konservasi Vol 12 No 1 (2007): Media Konservasi
Publisher : Department of Forest Resources Conservation and Ecotourism - IPB University

Pengukuran estron terkonjugasi (E1C) dan pregnanediol glucuronat (PdG) di feses dilakukan pada tiga ekor Owa Jawa (Hylobates moloch AUDEBERT 1797) betina untuk mengevaluasi fungsi ovarium ketiga owa tersebut yang masing-masing dipelihara di kandang individu di Pusat Studi Satwa Primata IPB dan di Taman Margasatwa Ragunan, Jakarta.. Contoh feses dikoleksi secara regular pada jam 07.00-09.00, 5-7 kali seminggu selama 4-9 bulan. Setelah melalui proses pengeringbekuan, contoh feses diekstraksi menggunakan metanol dan asai E1C dan PdG dilakukan dengan enzyme immunoassay (EIA). Profil hormon yang diperoleh menggambarkan adanya satu betina yang bersiklus dengan panjang siklus 21-25 hari. Lama fase folikular yang diperoleh pada betina tersebut bervariasi antara 11-18 hari, dan fase luteal yang relative konstan dengan lama 8-12 hari. Akan halnya dua betina yang lain, tidak diperoleh adanya gambaran pola yang menunjukkan siklus ovarium yang regular. Pembengkakan organ kelamin luar yang berfluktuasi juga hanya teramati pada betina yang bersiklus dengan lama pembengkakan 3-5 hari, dan perdarahan menstruasi terjadi selama 2-3 hari. Hasil yang diperoleh menunjukkan bahwa pengukuran steroid di feses merupakan suatu metoda nonivasif yang dapat diterapkan untuk mengevaluasi fungsi ovarium Owa Jawa. Keadaan fisiologis dari individual betina mungkin menjadi bahan pertimbangan lain dibandingkan tipe perkandangan sebagai faktor yang mempengaruhi profil hormonal.Kata kunci: Owa Jawa, steroid di feses, pembengkakan organ kelamin luar

Physiological Response of Bligon Buck to Transportation: Relation to Level of Thyroid Hormone Pudji Astuti; Sarmin -; Asmarani Kusumawati; Claude Mona Airin; Hera Maheshwari; Luthfiralda Sjahfirdi
Jurnal Veteriner Vol 11 No 2 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (115.212 KB)

Transportated animals may subject to a variety of physical stimuli including metabolism, crowding,noise, handling, isolation, agitation, and extreme temperature .The aim of this study was to determine thechanges of serum T4 and T3 concentration, during animals transportation. Six adult Bligon buck with bodyweight ranging from 26-30 Kg were used in this study. Two weeks prior to the experiment, the animalswere given anthelmintic Albendazole to eliminate egg worm. All animals were fed standard diet in theirpen at 10% of their body weight per head daily and commercial concentrate also given everyday. Freshwater was provided ad libitum. All animals were transported around village for 16 hours starting from18.00 pm until 10.00 am in open small truck (3 x 2 m); eye contact each others would be possible. Bloodsamples were withdrawn from jugular vein using vacutainer tubes containing heparin into 1.5 mL glasstubes, then centrifuged at 500 g for 15 minutes. Plasma was collected to be stored at –20° C. The bloodwere collected every 4 hours from 8 hours before transportation ( at 10.00 am, 14.00 pm and 18.00 pm)until the time of arriving after transportation at 10.00 am. Plasma was harvested and stored at –20° Cuntil T3 and T4 concentrations were measured using ELISA method (enzyme linked immunosorbentassay) product DRG, Germany. The result showed that transportation of Bligon bucks for 16 hours havean affect on level of T4 only (P<0.05) and not for T3 concentration (P>0.05) due to physical stimuli such ascrowding, heat stress, noise, handling would be discarded so that the metabolic process was stable. Duringtransportation, decreasing of T4 levels indicated conversion of T4 to T3 to form active hormone.

Fourier Transform Infrared Sebagai Metode Alternatif Penetapan Tingkat Stres pada Sapi (FOURIER TRANSFORM INFRARED AS AN ALTERNATIVE TOOL FOR DETERMINING OF STRESS IN COW) Pudji Astuti; Claude Mona Airin; Slamet Widiyanto; Amelia Hana; Hera Maheshwari; Luthfiralda Sjahfirdi
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (120.145 KB)

Stress in animal is a condition of nonspesific discomfort which cause of non-specific immune defects,failure of reproduction, and decreased of meat carcass until the death of animals. To determine stress ofcattle, it will be invented stress detector using a non-invasive method based on the spectroscopy FourierTransform Infrared (FTIR). Basically, FTIR will detect component in compound of cathecolamine andcortisol as ketone (= O) and methyl (= CH 3). Furthermore, each group of components will be detected indifferent of absorbant and wavelength. The results showed that average level of cortisol in female beefcattle durimg resting eriod was 38,48±21,53 ng/dL, on time of slaughtering were 116,88±112,59 ng/dL. Forbull, which were resting 20,42±9,25 ng/dL; when animal was slaughtered level of cortisol was 67,61±41,62ng/dL. Using FTIR, it was showed that compound of metil was absorbed well. Animals with udder stresscondition have been recorded on the wave lenght of 2777-3456 nm. It has been concluded level of cortisolon cattle which were resting is significantly different from animal which were slaughtering P(< 0.05),where cortisol would increase drastically. Using Calibration of FTIR indicated resting animals only havefewer value of absorbance than animals which slaughtered. FTIR is a very prospect method for makingstress indicator.

Pemanfaatan Electronic Nose sebagai Sensor Kimiawi Urin Guna Melacak Birahi Sapi (ELECTRONIC NOSE AS URINARY CHEMICAL SENSOR FOR DETERMINING ESTROUS PHASE IN CATTLE) Pudji Astuti; Claude Mona Airin; Slamet Widiyanto; Luthfiralda Sjahfirdi; Hera Maheshwari
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (206.873 KB)

The timing of artificial insemination relies largely on behavioral observation of estrus. The problemfaced is that not all cattle shows signs of estrus significantly, which affect the accuracy of insemination,and therefore,the success rate of Artificial Insemination is less than 50%. Recently, the determination ofestradiol levels as an indicator of estrus is done by observation of physical signs and doing ELISA test,which is expensive and provide longer time. In order to solve these problems, a tool estrus detector is madenamely Electronic Nose (EN). Determination of estrus with EN is cheaper because it does not need to usecomplexmaterials, just only use the samples. Mechanism of action of EN is using a sensor that is vaporized,while animals estrus will emit pheromones that are vaporized. Theaim of this study was to determinewhether the stage of estrus can be detected by using EN. Urine of female Ongole Crossbred which maintainedin Kuwang, district of Cangkringan, Yogyakarta, with BCS of 3 was used in this research. The sample wascollected shortly before injection of dinoprost as estrus synchronization then it repeated when cattle got estrus phase. The urinary sample of the estrus cattle was sensitive to methane, propane, butane, whereasin non-estrus cattle, besides the three of these component (methane, propane, butane), sensor was alsocaptured hydrogen sulfide. Furthermore, our electronic nose had been able to distinguish estrus phase andnon-estrus based on domain area.Thus, the Electronic Nose is very prospect used as a detector of estrus incattle. Hydrogen disulfide could possibly be used as an indicator comparison between cow estrus and nonestrus.

Pengaruh Kondisi Vasektomi pada Perilaku Reproduksi Monyet Ekor Panjang (Macaca fascicularis) di Yayasan Inisiasi Alam Rehabilitasi Indonesia, Bogor Hana Nabilah; Luthfiralda Sjahfirdi; Wendi Prameswari
JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI Vol 4, No 3 (2018)
Publisher : Universitas Al Azhar Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36722/sst.v4i3.282

Abstrak - Telah dilakukan pengamatan perilaku reproduksi moyet ekor panjang (Macaca fascicularis) di Yayasan Inisiasi Alam Rehabilitasi Indonesia (YIARI) yang telah disterilkan dengan metode vasektomi. Tujuan penelitian adalah mengamati pengaruh vasektomi monyet ekor panjang terhadap perilaku reproduksinya. Pengamatan dilakukan pada tiga ekor monyet ekor panjang jantan (Jantan1, Jantan2, dan Jantan3) yang dikandangkan bersama dua ekor monyet ekor panjang betina (Betina1 dan Betina2) dengan metode scan sampling dan ad libitum dengan interval waktu 5 menit tanpa jeda. Pengamatan dilakukan dari pukul 08.00-15.00 WIB selama 14 hari di bulan Agustus 2016, serta dengan total titik sampel yang diperoleh adalah 1008. Hasil pengamatan menunjukkan ketiga jantan masih aktif melakukan perilaku reproduksi, dengan Jantan1 yang merupakan jantan dominan dalam kelompok yang lebih mendominasi betina dibanding Jantan2 dan Jantan3, dibuktikan dengan nilai MM (Male Mounts) terhadap betina yaitu Jantan1 sebanyak 111 kali, Jantan2 sebanyak 10 kali dan Jantan3 sebanyak 21 kali. Perilaku reproduksi pada ketiga jantan masih ditemukan karena metode vasektomi tidak merusak proses spermatogenesis sehingga sistem hormonal hewan tidak terganggu. Terdapat perbedaan frekuensi perilaku reproduksi jantan terhadap Betina1 dan Betina2. Hal tersebut dikarenakan preferensi jantan dalam kelompok yang lebih memilih untuk kawin dengan Betina1 daripada Betina2, yang dibuktikan dengan nilai FRS (Female Refuses Sex) dari Betina1 terhadap jantan sebanyak 111 kali sementara Betina2 sebanyak 7 kali. Diduga Betina1 merupakan betina dominan dengan kadar hormon estrogen yang lebih tinggi daripada Betina2.Kata Kunci - Macaca fascicularis, Rehabilitasi, Perilaku Reproduksi, VasektomiAbstract - A study of reproductive behavior has been conducted on long tail macaques (Macaca fascicularis) which have been sterilized by a vasectomy method at Yayasan Inisiasi Alam Rehabilitasi Indonesia (YIARI), Bogor. The purpose of the study is to observe the influence of vasectomy to reproductive behavior of those animals. Observation was conducted on 3 (three) male macaques (the code names are: Jantan1, Jantan2 and Jantan3) which were captive-housed with 2 other female macaques (the code names are: Betina1 and Betina2). The method of observation is a combination of scan sampling and ad libitum methods with 5 (five) minutes intervals without pause. Daily observation were done from 08:00 am to 03:00 pm for 14 days in August 2016 and brought about 1,008 sample points. Results showed all males still actively performed reproductive behavior with Jantan1 in dominant position to all females compared to other males. Male Mounts (MM) of Jantan1 (111 times) was found higher than other males (Jantan2 10 times and Jantan3 21 times). We resume that reproductive behavior was still found in all males because vasectomy methods did not affect spermatogenesis process therefore the hormonal system remain in normal condition. There are differences in the frequency of males’ reproductive behavior to all females since all males preferring to mate with Betina1 instead of Betina2. The Female Refuse Sex behavior to males was found higher in Betina1 (111 times) than Betina2 (7 times) as Betina1 assumed to be dominant than Betina2.Keywords - Macaca fascicularis, Rehabilitation, Reproductive Behavior, Vasectomy

PEMERIKSAAN PROFIL HORMON PROGESTERON SELAMA SIKLUS ESTRUS TIKUS (Rattus norvegicus) BETINA MENGGUNAKAN PERANGKAT INFRAMERAH Luthfiralda Sjahfirdi; Putri Krida Gita P.; Pudji Astuti; Hera Maheshwari
Jurnal Kedokteran Hewan Vol 7, No 1 (2013): March
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan memperoleh profil fluktuasi kadar hormon ovari sepanjang siklus estrus. Sampel darah yang berasal dari sepuluh ekor tikus betina diambil selama tiga siklus estrus dan dianalisis menggunakan perangkat inframerah. Kadar progesteron selama tiga siklus memiliki kisaran 6-11 ng/ml pada masa estrus dan meningkat menjadi 32-49 ng/ml pada saat non-estrus.

APLIKASI FOURIER TRANSFORM INFRARED (FTIR) DAN PENGAMATAN PEMBENGKAKAN GENITAL PADA SPESIES PRIMATA, LUTUNG JAWA (Trachypithecus auratus) UNTUK MENDETEKSI MASA SUBUR Luthfiralda Sjahfirdi; Nikki Aldi; Hera Maheshwari; Pudji Astuti
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengetahui gugus fungsi penanda yang merepresentasikaan hormon metabolit E1C dan PdG serta bilangan gelombangnya pada urine yang didukung dengan pengamatan genitalia untuk memastikan pendeteksian masa subur pada lutung jawa (Trachypithecus auratus). Sampel urine dan pengamatan genitalia diperoleh dari 2 (dua) ekor lutung jawa betina di Pusat Primata Schmutzer, Kebun Binatang Ragunan, Jakarta. Sampel urin diambil setiap hari dan dilengkapi dengan pengamatan genitalia. Hasil menunjukkan bahwa gugus fungsi penanda yang merepresentasikan E1C dan PdG pada lutung jawa sama dengan yang teridentifikasi pada tikus namun dengan bilangan gelombang berbeda. Hormon metabolit E1C direpresentasikan melalui gugus fungsi alkil, aromatik, dan hidroksil pada bilangan gelombang 596 cm-1, 698 cm-1, 3599 cm-1, dan PdG direpresentasikan melalui gugus fungsi alkil dan aldehid pada bilangan gelombang 1450 dan 1699 cm-1. Dari penelitian ini dapat disimpulkan bahwa gugus fungsi penanda yang teridentifikasi oleh fourier transform infrared (FTIR) dapat berlaku secara universal, namun bilangan gelombang yang merepresentasikannya bersifat spesifik spesies.

Optimalisasi Prinsip-Prinsip Pancasila Untuk Konservasi yang Efektif Sebagai Strategi Mengurangi Kepunahan Spesies dan Mendorong Upaya Pelestarian Alam Hadi, Nestiyanto; Ainy, Noer Sarifah; Sjahfirdi, Luthfiralda; Pudjiastuti, Sri Rahayu; Andayani, Noviar; Islahuddin, Islahuddin
Jurnal Ilmiah Pendidikan Pancasila dan Kewarganegaraan Vol 9, No 2 (2024): Juli 2024
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um019v9i2p201-210

Co-Authors Ainy, Noer Sarifah Amelia Hana Ananda, Syafira Dwi Andayani, Noviar Asmarani Kusumawati Bambang Purwantara Claude Mona Airin Dondin Sajuthi ERIC HAYES Firmanza, Nasya Aqila Hadi S Alikodra Hadi, Nestiyanto Halim, Nauhal Nazri Hana Nabilah HERA MAHESHWARI Indriastuti, Hapsari Islahuddin, Islahuddin Mozes R. Tolihere Nikki Aldi Oriza Savitri Ariantie PUDJI ASTUTI Pudji Astuti Pudji Astuti Pudji Astuti Pudji Astuti Pudjiastuti, Sri Rahayu Putri Krida Gita P. Reviany Widjajakusuma Reviany Widjajakusuma Rosame, Ilmia Saka, Raisa Tatum Saka, Raisa Tatum Sarmin - Slamet Widiyanto Sukmaningrum, Anisa Putri Tatang Mitra Setia Tuty Laswardi Yusuf Wendi Prameswari Zain, Siti Nursheena Mohd

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