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Mitra Slipranata
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Agriculture, Biological Sciences & Forestry Veterinary

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Staphylococcus aureus Penghasil Pigmen Kuning yang Diisolasi dari Kejadian Bumblefoot pada Broiler Lebih Patogen Dibanding Penghasil Pigmen Putih (STAPHYLOCOCCUS AUREUS PRODUCING YELLOW PIGMENT ISOLATED FROM BUMBLEFOOT CASE IN BROILER CHICKENS IS MORE PAT Khusnan .; Wahyu Prihtiyantoro; Mitra Slipranata
Jurnal Veteriner Vol 15 No 4 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study to evaluate the correlation between the pigments produced by Staphylococcus aureusisolateswith their adhesion capability on epithelial cells and phagocytes activity of macrophages in vitro.Ten S.aureus isolatesfrom bumble foot and arthritis casesin broiler were used in this study. The adhesion assaywas performed using epithelial cell derived from human buccal epithelial cells whereas phagocytic activitywas conducted using mouse peritoneal macrophage cells.The results showed that S. aureus isolates frombroiler produced both yellow and white pigments. Staphylococcus aureus producing white pigment adheredto human buccal epithelial cells with the density of about 24,25 bacteria/cell, which were higher than thoseof Staphylococcus aureus producing yellow pigmentabout 23,78 bacteria/cell. Staphylococcus aureusproducing white pigment was more phagocytosed by macrophages than S. aureusproducing yellow pigment,with an average phagocytic activiry of19,86 bacteria/cellas compared to15,96of bacteria/cell, respectively.
Diagnosis Infeksi Streptococcus suis serotipe-2 pada Babi Secara Serologi dengan Muramidase Released Protein (SEROLOGICALLY DIAGNOSE OF STREPTOCOCCUS SUIS SEROTYPE-2 INFECTION IN PIGS BASED ON MURAMIDASE RELEASED PROTEIN) Siti Isrina Oktavia Salasia; Clara Ajeng Artdita; Mitra Slipranata; Sidna Artanto
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Streptococcus suis is a bacterial pathogen causing disease of pigs that characterized by meningitis,bronchopneumonia, arthritis, pericarditis, polyserositis and septicaemia. S. suis especially serotype 2 caninfect human (zoonotic) with a special symptom of meningitis. The aim of this research was to detect S.suis infection based on muramidase released protein (MRP), as an important virulence marker of S. suis.S. suis serotype 2 strain P171 with phenotype of MRP+EF+ was used in this research. The MRP antigen wasextracted using lysozyme and separated by using sodium dodecyl sulphate-polyacrylamide gel electrophoresis(SDS-PAGE). Balb/c mice were imunized with 136 kDa MRP to produce antibody against MRP. Theantibody was evaluated by using enzyme linkage immunosorbent assay (ELISA). The results of the researchshowed that the antibody against MRP with molecular weight of 136 kDa could be produced on Balb/Cmice with the highest absorbance of 3,889 and could be used to detect field sera from infected pigs with200x dilution using ELISA antigen capture. Antibody against MRP could detect serologically of S. suisinfection in pigs in Papua with 50% seropositivy by using ELISA antigen capture and 40% by using dot blot.
KARAKTERISASI FAKTOR VIRULENSI Escherichia coli PATOGEN ZOONOTIK (O157:H7) ISOLAT ASAL TINJA SAPI POTONG CHARACTERIZATION OF Escherichia coli VIRULENCE FACTORS OF ZOONOTIC PATHOGENS (O157:H7) ISOLATE OF FECAL CATTLE Wahyu Prihtiyantoro; Hartatik Hartatik; Khusnan Khusnan; Mitra Slipranata; Fatkhanudin Aziz
Agros Journal of Agriculture Science Vol 16, No 2: Edisi Juli 2014
Publisher : Fakultas Pertanian, Universitas Janabadra

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Kemampuan hemaglutinasi, keberadan hemolisin dan kekebalan terhadap antibiotik merupakan faktor virulensi yang penting pada Escherichia coli. Penelitian ini bertujuan melakukan karakterisasi faktor virulensi terhadap 9 (sembilan) E. coli isolat sapi potong yang terdiri 5 (lima) isolat berasal dari tinja dan 4 (empat) isolat berasal dari pupuk kandang. Duapuluh dua persen isolat mampu menggumpalkan eritrosit dan 100 persen isolat tidak memiliki hemolisin, 4 isolat asal tinja positif sebagai patogen zoonotik (O157:H7). Kekebalan isolat oleh eritromisin, metisilin, penisilin, tetrasiklin dan gentamisin  masing-masing 66,7 persen, 66,7 persen, 66,7 persen, 16,7 persen dan 0 persen. Resistensi hanya terjadi pada isolat asal tinja sedangkan isolat asal pupuk kandang masih sensitive terhadap antibiotika-antibiotika tersebut. Hemagglutination ability, the existence of hemolysin and antibiotic resistance is an important virulence factor in Escherichia coli. This study aimed to characterize the virulence factors of the nine (9) E. coli isolates beef cattle comprising five (5) isolates from feces and 4 (four) isolates from manure. Twenty-two percent of isolates were able to aglutinate erythrocytes and 100 percent of isolates did not have hemolysin. Immunity isolates by erythromycin, methicillin, penicillin, tetracycline and gentamicin respectively 66.7 percent, 66.7 percent, 66.7 percent, 16.7 percent and 0 percent. Resistance occurs only at the fecal isolates while isolate of manure is still sensitive to the antibiotics
Co-Authors Clara Ajeng Artdita Fatkhanudin Aziz Hartatik Hartatik Khusnan k Sidna Artanto Siti Isrina Oktavia Salasia Wahyu Prihtiyantoro