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Diagnosis Infeksi Streptococcus suis serotipe-2 pada Babi Secara Serologi dengan Muramidase Released Protein (SEROLOGICALLY DIAGNOSE OF STREPTOCOCCUS SUIS SEROTYPE-2 INFECTION IN PIGS BASED ON MURAMIDASE RELEASED PROTEIN) Siti Isrina Oktavia Salasia; Clara Ajeng Artdita; Mitra Slipranata; Sidna Artanto
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (135.267 KB)

Abstract

Streptococcus suis is a bacterial pathogen causing disease of pigs that characterized by meningitis,bronchopneumonia, arthritis, pericarditis, polyserositis and septicaemia. S. suis especially serotype 2 caninfect human (zoonotic) with a special symptom of meningitis. The aim of this research was to detect S.suis infection based on muramidase released protein (MRP), as an important virulence marker of S. suis.S. suis serotype 2 strain P171 with phenotype of MRP+EF+ was used in this research. The MRP antigen wasextracted using lysozyme and separated by using sodium dodecyl sulphate-polyacrylamide gel electrophoresis(SDS-PAGE). Balb/c mice were imunized with 136 kDa MRP to produce antibody against MRP. Theantibody was evaluated by using enzyme linkage immunosorbent assay (ELISA). The results of the researchshowed that the antibody against MRP with molecular weight of 136 kDa could be produced on Balb/Cmice with the highest absorbance of 3,889 and could be used to detect field sera from infected pigs with200x dilution using ELISA antigen capture. Antibody against MRP could detect serologically of S. suisinfection in pigs in Papua with 50% seropositivy by using ELISA antigen capture and 40% by using dot blot.
PENGARUH KARAKTERISTIK PETERNAK TERHADAP ADOPSI TEKNOLOGI PEMELIHARAAN PADA PETERNAK KAMBING PERANAKAN ETTAWA DI DESA HARGOTIRTO KABUPATEN KULON PROGO NURULIA HIDAYAH; CLARA AJENG ARTDITA; FAJAR BUDI LESTARI
Jurnal Bisnis dan Manajemen (Journal of Business and Management) Vol 19, No 1 (2019)
Publisher : Magister Manajemen Fakultas Ekonomi dan Bisnis UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbm.v19i1.30916

Abstract

Livestock in Indonesia are mostly smallholder farms which have the following characteristics, including small scale businesses and using simple technology. One effort to overcome these problems is to carry out technological transformation. The introduction of technology for the maintenance of Peranakan Ettawa (PE) goats has been given to farmers, but not many of these technologies have been adopted by farmers who have certain characteristics. This study aims to analyze the characteristics of farmers who influence the technology adoption rates of Ettawa Peranakan goat breeding. The materials used were Peranakan Ettawa breeders who were members of the Mantep Makaryo, Subur and Tirto livestock groups in the village of Hargotirto, Kokap District, Kulon Progo Regency. The method used in this study is the survey method. The study was conducted from April to October 2018. The analysis used in this study is multiple linear regression analysis, namely the analysis used to determine the factors that influence the rate of technology adoption. The total respondents used in this study were 74 breeders. The results of the analysis showed that at the same time the characteristics of breeders, age, education level, number of family dependents, experience of breeding, number of lactation PE goats, and scale of ownership of PE goats had an influence on 67.20% goat maintenance technology adoption. Partially showing a significant effect (P <0.01) is the experience of farming, education level (P <0.05) while the number of dependents shows the effect on technology adoption (P <0.1). Keywords: Adoption, Characteristics of breeders, PE Goats
Faktor resiko mastitis subklinis pada Kambing Peranakan Etawah di Kecamatan Kokap, Kulonprogo, Yogyakarta Morsid Andityas; Clara Ajeng Artdita; Nur Ika Prihanani
Livestock and Animal Research Vol 18, No 3 (2020): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (434.54 KB) | DOI: 10.20961/lar.v18i3.41273

Abstract

Tujuan: Mastitis merupakan suatu peradangan yang terjadi pada glandula intramamari sebagai respon dari mekanisme pertahanan tubuh. Kejadian dan tingkat infeksi mastitis sangat dipengaruhi oleh berbagai macam faktor penyebab. Penelitian ini bertujuan untuk mengidentifikasi faktor-faktor resiko yang berhubungan dengan kejadian mastitis subklinis pada kambing peranakan etawah (PE) di Kecamatan Kokap, Kulonprogo, Yogyakarta.Metode:Jenis metode yang digunakan pada penelitian ini adalah metode survei. Teknik sampling yang digunakan dalam penelitian ini adalah purposive sampling dengan jumlah 200 sampel susu segar dari kambing PE. Pengumpulan data dilakukan dengan metode wawancara (kuesioner), pengamatan langsung dan pengujian status mastitis dengan menggunakan reagen California Mastitis Test (CMT). Data penelitan ini dianalisis secara diskriptif dan analisis bivariat chi square (X2), Odds Ratio (OR), serta Relative risk (RR).Hasil: Hasilpenelitian menunjukan bahwa faktor resiko yang berperan terhadap kejadian mastitis subklinis di Kecamatan Kokap adalah variabel umur sapih <2 bulan (X2=22,99; OR=21,13 dan RR=13,2) dan jarak pembuangan limbah <15 m dari kandang (X2=3,98; OR=6,52 dan RR=5,44).Kesimpulan: Variabel faktor resiko umur sapih <2 bulan dan jarak pembuangan limbah <15 m dari kandang memiliki asosiasi kuat dengan infeksi mastitis subklinis di Kecamatan Kokap, Kulonprogo, Yogyakarta.