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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences Dinamisia: Jurnal Pengabdian Kepada Masyarakat Biosaintifika: Journal of Biology & Biology Education Jurnal Riset Akuakultur Jurnal Perikanan Pantura Jurnal Kemaritiman: Indonesian Journal of Maritime Jurnal Perikanan
Purnama Sukardi
Universitas Jenderal Soedirman
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Environmental Science Social Sciences Other

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Survival Rate, Growth And Chemical Content of Dendronereis pinnaticirris (Polychaeta, Nereidae) In Maintenance With Different Food And Substrate Eko Setio Wibowo; Edy Yuwono; Purnama Sukardi; Asrul Sahri Siregar
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 25, No 2 (2020): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (216.811 KB) | DOI: 10.14710/ik.ijms.25.2.75-84

Abstract

The worm Dendronereis pinnaticirris is used as feed of shrimp broodstock in a hatchery, mainly because of its availability in the local market, and its nutritional content required for improving gonad maturation and post larvae production. The important economic value of the worm and the increasing demand for feed in shrimp hatcheries have led to an intense exploitation that suppers its population and the sustainability of the whole estuarine ecosystem. The study, which represents the starting point of large-scale production of the polychaete worm by culture in the artificial system, shall be undertaken. Accordingly, a production study using D. pinnaticirris juvenile was carried out under controlled conditions fed with two different feed (feed contains mainly plant protein and animal protein, respectively), and kept in three different substrates (substrate consists of mud and 8.78%, 37.34%, 39.17% sand, respectively). The treatments were arranged according to randomized completely block design in 8 (eight) replicates. The survival rate, body weight increment and growth, oxygen consumption, proximate body chemical, and fatty acid contents were measured. The results showed that growth and oxygen consumption was significantly influenced by a substrate and feed type (P<0.05).  Worms on the mud substrate with 39.17% sand, and feed containing vegetable protein showed the highest oxygen consumption.  Survival rate and chemical body content were not significantly influenced by the type of substrate and feed (P<0.05). The protein content of the worm was 32.02-43.81%, while fat content was 2.41-9.89%. Twenty different fatty acids were identified in the worm of all treatment groups. 
MIKROENKAPSULASI EKSTRAK KASAR MAGGOT SEBAGAI PAKAN SUBSTITUSI PADA PENYAPIHAN PAKAN LARVA IKAN NILA (Oreochromis niloticus) Himawan Prasetiyo; Sri Marnani; Purnama Sukardi
Jurnal Kemaritiman: Indonesian Journal of Maritime Vol 1, No 2 (2020): Desember 2020
Publisher : Universitas Pendidikan Indonesia (UPI) Kamous Serang

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Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh penyapihan pakan mikroenkapsulasi ekstrak kasar maggot pada umur larva nila (Oreochromis niloticus) yang berbeda serta menentukan waktu penyapihan yang tepat. Analisis yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan 4 perlakuan (kontrol atau tanpa penyapihan, penyapihan pada umur 9, 14 dan 19 hari) dan 4 ulangan. Variabel yang diamati yaitu, pertumbuhan mutlak (panjang dan berat), laju pertumbuhan spesifik (SGR) dan sintasan (SR). Hasil penelitian menunjukan pertumbuhan mutlak panjang berkisar 7.15 - 8.65 mm, pertumbuhan mutlak berat berkisar 0.046 - 0.084 g, SGR berkisar 5.633 - 7.352 % dan SR berkisar 64 - 76 %. Waktu penyapihan pakan berpengaruh terhadap pertumbuhan dan sintasan larva ikan nila (p0,05). Waktu penyapihan pakan terbaik terdapat pada usia larva 9 hari.
PRESERVASI BEKU SPERMATOZOA IKAN CUPANG (Betta splendens) STRAIN HALF-MOON DALAM MADU DAN NACL FISIOLOGIS Rika Prihati Cahyaning Pertiwi; Isdy Sulistyo; Purnama Sukardi
Jurnal Perikanan Pantura (JPP) Vol 4 No 2 (2021): SEPTEMBER 2021
Publisher : Universitas Muhammadiyah Gresik

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30587/jpp.v4i2.3040

Abstract

A study entitled “Chilled Preservation of Spermatozoa Half-moon Strain Fighting Fish (Betta spendens) in Honey and Physiological NaCl”, was conducted to determine preserved spermatozoa motility and viability of Fighting Fish in honey and NaCl solutions, under chilling temperature (-250C). The study applied Completely Randomized Design (CRD) to examine three treatments, i.e aquadest, 0.09% NaCl and honey extenders, with quantuplicates. Data, being spermatozoa motility (%) and viability (time in second), were F-tested (ANOVA) and followed by LSD test (P<0.01). After storage of 7 days, the result showed that spermatozoa motility did not differ between treatments, however the viability of spermatozoa were significantly different (P<0.01). The highest viability was observed in sperm stored in extender Honey averaging 368.90±102.16 seconds, compared to the 0.09% NaCl extender (81.79±8.54 seconds), and aquadest (187.90±35.36 seconds). It was concluded that the Honey, NaCl 0.09% and aquadest as extenders could be used as preservation solution fighting fish spermatozoa. The best viability of spermatozoa was observed in honey extender.
Peningkatan Keterampilan Petani pada Usaha Budidaya Ikan Lele dengan Teknologi Kawin Suntik Norman Prayogo; Purnama Sukardi; Asrul Sahri Siregar
Dinamisia : Jurnal Pengabdian Kepada Masyarakat Vol. 6 No. 6 (2022): Dinamisia: Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/dinamisia.v6i6.7825

Abstract

Answering higher challenge of occupational area along with smaller available job opportunities, it is needed business creation for the society based on local resources and science and technology. One of aquaculture activity that conducted was application of injected spawning technique among and rearing larvae of catfish. The objective of this activity, skill, and increase income also as a new economic activity for students in Farmer Group Mandiri Jaya, Sokaraja, Banyumas. Research method comprised of activities like transfer of knowledge, training, demonstration plot and application of production for 4 months. Evaluation of technology transfer activity viewed from questionnaire value, training and demonstration plot viewed from ability and of participant to produce seed and profit gained. Generally, knowledge and skill of participant was improved. However viewed from production yield was still varied Evaluasi kegiatan transfer teknologi dilihat dari nilai kuisioner , pelatihan dan percontohan dilihat dari kemampuan dan keterampilan penguasaan teknologi sedangkan aplikasi produksi adalah kemampuan peserta menghasilkan benih dan keuntungan yang diperoleh. Secara umum pengetahuan, keterampilan peserta meningkat. Namun ditinjau dari segi hasil produksi masih bervariasi.
Digestive Enzyme Activities of Osteochilus vittatus with Spirulina platensis Feed Supplementation in Biofloc System Rizka Yunida; Purnama Sukardi; Sorta Basar Ida Simanjuntak
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 3 (2019): December 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i3.19108

Abstract

Osteochilus vittatus is a freshwater fish that needs to be developed because it tastes savory and can be used as a biocleaning agent. Research on the effect of supplementation of Spirulina platensis in nilem fish that is maintained in a biofloc system on digestive enzyme activity has never been done before. The objectives of this study were to determine the effect of S. platensis supplementation on Osteochilus vittatus digestive enzyme activity maintained in the biofloc system and to obtain the most optimum level of S. platensis supplementation in improving the  digestive enzymes activity. The study was conducted experimentally with Completely Randomized Design with four treatments and five replications. Osteochilus vittatus were fed with Spirulina platensis level of 0, 2, 4, and 6 g kg-1 for 56 days. Enzyme activity was measured on days 0 and 56 with three kinds of buffer solutions. Amylase and protease activity was analyzed using ANOVA with a confidence level of 95%. The results showed that the highest amylase activity in the liver was 4.764 ± 1.705 U mg-1 protein and proximal intestine was 2.328 ± 0.838 U mg-1 protein. The highest protease activity was found in the liver and proximal intestine with activity of 6.536 ± 0.911 U mg-1 protein and 6.207 ± 2.195 U mg-1protein. Spirulina platensis supplementation level of 6 g kg-1 feed is the most optimum level (p0.05). Cultivation of fish with S. platensis supplementation in feed can increase enzyme activity and the fish maintenance in biofloc systems can improve water quality.
Assistance in Independent Fish Feed Production at POKDAKAN Mina Mandiri, Panembangan Village Banyumas: Pendampingan Pembuatan Pakan ikan Mandiri Pada POKDAKAN Mina Mandiri Desa Panembangan Banyumas Purnama Sukardi; Mustika Palupi; Rudy Wijaya; Ren Fitriadi; Kothoh Syuraikhanah; Indra Hariman; Ufianah Ufianah
Dinamisia : Jurnal Pengabdian Kepada Masyarakat Vol. 8 No. 1 (2024): Dinamisia: Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/dinamisia.v8i1.15989

Abstract

The purpose of this activity is to improve the competence of fish feed manufacturing and can increase fish sales production. The approach method carried out in this activity is a participatory Participatory Rural Appraisal (PRA) approach starting with a survey and problem identification, socialization and counseling training on making fish meal, training and direct technical guidance on making feed formulations, and assistance in making fish feed. The result of this dedication is that partners know the technology of making feed using available and abundant ingredients so as to reduce production expenditure in fish farming. Counseling and training activities in community service received good enthusiasm from members and partners of direct participants in making fish feed. The result of service activities is that partners gain knowledge and skills in making independent fish feed from waste and local raw materials. Making independent feed is able to increase production profits by reducing production costs incurred. The potential for developing activities can encourage productivity and provision of skills that can be disseminated to the wider community.
ISOLASI, IDENTIFIKASI, DAN ANALISIS EKSPRESI GEN PENGKODE GROWTH HORMONE PADA IKAN SIDAT (Anguilla bicolor) Hamdan Syakuri; Satrio Haryu Wibowo; Anandita Ekasanti; Petrus Hary Tjahja Soedibya; Sri Marnani; Emyliana Listiowati; Dewi Nugrayani; Purnama Sukardi
Jurnal Riset Akuakultur Vol 19, No 1 (2024): (Maret 2024)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.19.1.2024.69-83

Abstract

Gen hormon pertumbuhan (growth hormone, GH) perlu dipelajari untuk mendukung domestikasi ikan sidat Anguilla bicolor di Indonesia. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi gen pengkode GH serta menganalisis ekspresinya pada sampel ikan sidat A. bicolor. Primer untuk amplifikasi gen GH ikan sidat A. bicolor didesain berdasarkan sekuen gen GH dari beberapa spesies ikan sidat lain yang tersedia di GenBank. Sampel cDNA hipofisa dan otak ikan sidat digunakan untuk amplifikasi gen GH. Hasil amplifikasi disekuensing dan hasilnya dianalisis menggunakan analisis BLAST, multiple sequences alignment, hormon signature, dan filogenetik. Analisis ekspresi gen GH dilakukan menggunakan teknik realtime PCR dengan metode delta-delta CT pada 14 sampel ikan sidat (23,1 ± 19,6 g; 24,5 ± 4,2 cm). Fragmen DNA sepanjang 486 bp berhasil diamplifikasi dan disekuensing. Sekuen gen GH A. bicolor memiliki similaritas nukleotida sebesar 98,49-99,14% jika dibandingkan dengan gen GH ikan sidat A. anguilla, A. australis, dan A. japonica. Sekuen parsial tersebut secara in silico diketahui mengkode bagian dari protein GH sepanjang 155 asam amino (aa). Sekuen asam amino protein GH A. bicolor sangat mirip dengan sekuen spesies ikan sidat lain dengan hanya tiga perbedaan asam amino dan membentuk satu percabangan pada pohon filogenetik. Tingkat ekspresi gen GH pada sampel ikan sidat memiliki variasi yang tinggi. Hasil penelitian ini dapat menjadi dasar untuk studi selanjutnya khususnya berkaitan dengan peran gen GH dalam pertumbuhan ikan sidat A. bicolor.The growth hormone (GH) gene needs to be studied to support the domestication of the Anguilla bicolor eel in Indonesia. This study aimed to isolate and identify the GH coding gene and analyze its expression in samples of the eel A. bicolor. Primers for amplification of the GH gene of A. bicolor eels were designed based on GH gene sequences from several other eel species available in GenBank. Pituitary and brain cDNA samples of eel were used for GH gene amplification. The amplification results were sequenced and the results were analyzed using BLAST analysis, multiple sequence alignment, hormone signature, and phylogenetic analysis. The GH gene expression analysis was carried out using the real-time PCR technique with the delta-delta CT method on 14 eel samples (23.1 ± 19.6 g; 24.5 ± 4.2 cm). The 486 bp DNA fragment was successfully amplified and sequenced. The GH gene sequence of A. bicolor has a nucleotide similarity of 98.49-99.14% when compared with the GH gene of other eel species A. anguilla, A. australis, and A. japonica. This partial sequence was found in silico to code 155 amino acids (aa) GH protein. The amino acid sequence of the A. bicolor GH protein is very similar to that of other eel species with only three amino acid differences and forms one branch on the phylogenetic tree. The expression level of the GH gene in eel samples had high variations. The results of this study could be a basis for further studies, especially regarding the role of the GH gene in the growth of the eel A. bicolor. 
ANALISIS HUBUNGAN SERTIFIKASI EKOLABEL TERHADAP MUTU DAN PRODUKSI UDANG VANNAMEI (Litopenaeus vannamei) PT YSI, BUTON TENGAH Fatah Khoerudin; Hary Tjahja Soedibya; Purnama Sukardi; Taufik Budhi Pramono; Endang Hilmi
Jurnal Perikanan Unram Vol 14 No 1 (2024): JURNAL PERIKANAN
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jp.v14i1.753

Abstract

Ecolabel certification is one of the requirements that must be met to export vannamei shrimp (Litopenaeus vannamei) with European and American destination countries. The research method uses a mix method (qualitative and quantitative). The sampling technique uses the Global Aquaculture Alliance-Best Aquaculture Practices (GAA-BAP) checklist. Data were collected using observation techniques, in-depth interviews, and literature studies. Data analysis uses descriptive analysis with primary and secondary data approaches. The total value of GAA-BAP application in PT YSI's vannamei shrimp pond for the critical questions category and the scored questions category is above the minimum percentage of achievement of GAA-BAP ecolabel certification by obtaining a total value of 96.4% and 90.6%. The results of quality testing of vannamei shrimp cultivated by PT YSI show that vannamei shrimp meet the criteria for GAA-BAP ecolabel certification. PT YSI's cultivation performance is in accordance with the company's target by obtaining an average harvest of 2.1 tons/pond and a total harvest of 61,488 kg/30 ponds with a productivity value of 50.5 tons/ha of 20m² diameter ponds. This can be achieved with FCR values of 1.68, SR 92.80%, ADG 0.26, MBW 22.8 gr, average size 43 and total feed 103,603 kg. The average water quality value is DO 5.12 mg/l, temperature 32 °C, salinity 38 ppt and pH 7.8.
Co-Authors Asrul Sahri Siregar Dewi Nugrayani Edy Yuwono Ekasanti, Anandita Eko Setio Wibowo Emyliana Listiowati Endang Hilmi Fatah Khoerudin Hamdan Syakuri Hary Tjahja Soedibya Himawan Prasetiyo Indra Hariman Isdy Sulistyo Kothoh Syuraikhanah Mustika Palupi Norman Prayogo Petrus Hary Tjahja Soedibya Ren Fitriadi Rika Prihati Cahyaning Pertiwi Rizka Yunida Rudy Wijaya Satrio Haryu Wibowo Sorta Basar Ida Simanjuntak Sri Marnani Taufik Budhi Pramono Ufianah Ufianah