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All Journal HAYATI Journal of Biosciences Bumi Lestari Reaktor Jurnal Presipitasi : Media Komunikasi dan Pengembangan Teknik Lingkungan METANA Microbiology Indonesia Journal of Engineering and Technological Sciences Jurnal Ilmiah Arena Tekstil Jurnal Teknik Lingkungan Manfish Journal
EDWAN KARDENA
Water and Wastewater Engineering Research Group, Faculty of Civil and Environmental Engineering, Institut Teknologi Bandung
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology

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PEMANFAATAN MIKROALGA AMOBIL SEBAGAI ADSORBEN PADA PENYISIHAN ZAT WARNA REACTIVE BLUE 4 (RB4) DAN REACTIVE RED 120 (RR120) DALAM LIMBAH CAIR TEKSTIL Edwan Kardena; Malinda Syifa Yusharani
Arena Tekstil Vol 37, No 2 (2022)
Publisher : Balai Besar Tekstil

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31266/at.v37i2.7777

Abstract

Pengolahan limbah zat warna dapat dilakukan dengan proses adsorpsi, namun mengalami keterbatasan biaya akibat harga adsorben yang mahal. Salah satu bahan baku adsorben potensial yang dapat digunakan adalah mikroalga. Penelitian ini menjelaskan proses pemanfaatan mikroalga dalam bentuk amobil sebagai adsorben untuk penyisihan Reactive Blue 4 (RB4) dan Reactive Red 120 (RR120). Alga yang digunakan berupa alga blooming dari Instalasi Pengolahan Air Limbah (IPAL) Bojongsoang yang dilakukan proses imobilisasi menggunakan silika. Adsorben diuji menggunakan FTIR, pH pzc, BET, dan SEM-EDS. Proses adsorpsi dilakukan dalam sistem batch (variasi perlakuan awal, pH, massa adsorben, dan waktu kontak) dan dalam reaktor kolom (variasi konsentrasi awal). Parameter yang diukur yaitu efisiensi penyisihan, model isoterm (Langmuir/Freundlich), kinetika reaksi (orde satu semu/orde dua semu), dan model kurva breakthrough. Hasil sistem batch menunjukkan kondisi adsorpsi terbaik terjadi menggunakan mikroalga-NaOH saat kondisi larutan pada pH 2 selama 4 jam dengan massa adsorben RB4=2,5 gram dan RR120=2 gram. Efisiensi penyisihan RB4 dan RR120 mencapai 98,58% dan 98,56%. Model isoterm cenderung mengikuti isoterm Langmuir dengan kinetika reaksi orde dua semu. Hasil sistem kolom menunjukkan adanya kenaikan konsentrasi awal memberikan waktu breakthrough yang lebih cepat dan kurva breakthrough paling sesuai dijelaskan menggunakan model Thomas dan Yoon-Nelson. Hasil adsorpsi RB4 dan RR120 menggunakan mikroalga amobil memberikan hasil yang lebih baik bila dibandingkan dengan karbon aktif dalam kondisi yang serupa. 
STUDI TINGKAT KETERURAIAN PEWARNA TEKSTIL MENGGUNAKAN LAKASE MURNI DARI Marasmiellus palmivorus Ragil Putri Widyastuti; Sri Harjati Suhardi; Dani Permana; Khomaini Hasan; Edwan Kardena; Agus Jatnika
Jurnal Kelautan, Lingkungan, dan Perikanan Vol 1 No 1 (2020): MANFISH JOURNAL
Publisher : Jurusan Ilmu Kelautan dan Perikanan Politeknik Negeri Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (801.26 KB) | DOI: 10.31573/manfish.v1i01.35

Abstract

Pewarna di dalam limbah tekstil menjadi salah satu aspek penting karena sulit didegradasi. Enzim lakase yang berasal dari jamur pelapuk putih Marasmiellus palmivorus terbukti efektif dalam mendegradasi beberapa jenis pewarna tekstil. Lakase murni diujikan pada pewarna tekstil dengan metode uji cepat atau High Throughput Screening (HTS). Sampel pewarna yang digunakan yaitu 14 pewarna jenis azo, anthrakuinon dan disperse. Uji cepat dilakukan pada suhu ruang menggunakan microplate titer dengan perbandingan sampel dan enzim 1:1 selama 24 jam dengan konsentrasi pewarna 150ppm. Sampel yang diamati merupakan campuran pewarna dan enzim murni dengan aktifitas spesifik 2,49 U/mg. Metode HTS memperlihatkan Procion Blue H-GN dan Levafix Blue E-Ra Gran positif terdekolorisasi oleh lakase. Screening dekolorisasi pewarna oleh kultur jamur M. palmivorus pada medium PDA juga dilakukan dengan hasil 7 pewarna terdekolorisasi yaitu Telon Blue AFN, Telon Red AFG, Isolan Silver N, Telon Blue BRL, Levafix Blue PN-3R, Procion Blue H-GN dan Levafix Blau E-Ra Gran. Dua warna yang positif terdegradasi oleh lakase dan kultur jamur diukur perubahan konsentrasinya berdasarkan nilai absorbansinya menggunakan spektrofotometer. Konsentrasi pewarna Levafix Blue E-Ra Gran turun hingga 86 ppm setelah 24 jam. Kesimpulan dari penelitian ini adalah metode HTS berhasil memperlihatkan sifat dekolorisasi berbagai pewarna tekstil dengan cepat dan terbukti dengan berubahnya konsentrasi pewarna setelah pengujian.
Progress and Challenges of Biological Leaching of Heavy Metal in Coal Ash from a Power Plant Yuni Lisafitri; Edwan Kardena
Journal of Engineering and Technological Sciences Vol. 55 No. 1 (2023)
Publisher : Directorate for Research and Community Services, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.eng.technol.sci.2023.55.1.8

Abstract

Bioleaching is a technique for reducing the heavy metal content of coal ash by using bacteria, fungi, or yeast. Previous studies in heavy metal bioleaching of coal ash discussed the factors affecting the process, but as yet there is little information on the challenges of using microorganisms. Therefore, this study aimed to obtain comprehensive information regarding the use of microorganisms in heavy metal bioleaching. Heavy metal concentrations in coal ash are low, and the metals are diverse. The components of coal ash are complexes that cannot leach certain heavy metals according to previous studies. These low concentrations and complex components make it difficult to investigate the bioleaching mechanism. The combination of biological and chemical interactions involves various components in this system. The high concentration of iron and heavy metal leached could be toxic for microorganisms. The process is influenced by several factors, such as particle size, pH, and pulp density. Most heavy metal bioleaching studies on coal ash have been conducted on a small scale to control conditions affecting the process. Bioleaching kinetics in coal is a liquid-solid reaction that can be represented by the shrinking core model, which was mainly used in this study.
Biotransformation of the Textile Dye Reactive Black 5 with Aspergillus niger and Analysis of Ligninolytic Enzyme Activity Prawitasari, Diah Ayu; Kardena, Edwan
HAYATI Journal of Biosciences Vol. 33 No. 2 (2026): March 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.2.297-309

Abstract

Removal of textile dye using biological processes is considered more cost-effective, generates no hazardous byproducts, and is ecologically friendly. This study analyzes the removal of the synthetic dye Reactive Black 5 (RB5) using the biological agent Aspergillus niger. The Treatment occurred in a 250 mL Erlenmeyer flask containing a mixture of dye solution. The preliminary research treatment involved varying dye concentrations, which were then analyzed by examining enzyme activity and fungal growth at different shaker rotations. The initial findings on concentration variation indicated that it was effectively removed up to approximately 90%. Further research determined that the highest concentration, 200 ppm, was used. The results obtained with rotation variations up to the sixth day were 95% for 100 rpm and 93% for 150 rpm. Dye removal utilizes laccase, lignin peroxidase (LiP), and manganese peroxidase (MnP) as degrading enzymes. The highest amount of enzyme activity was achieved at 100 rpm, corresponding to the most effective dye removal. The enzyme activity at the 100-rpm variation was observed for MnP (62.2 U/L), LiP (32.2 U/L), and laccase (16.6 U/L). The rapid growth of the Aspergillus niger fungus is associated with the biomass, which influences the biosorption mechanism. Biodegradation pathways indicate the cleavage of aromatic bonds, with toluene and propanoic acid identified as the final products.
Co-Authors . WISJNUPRAPTO Aghnia, Dini Widyani Agus Jatnika Amalia Muchammad Amanda Larasati Aminudin Sulaeman Aminudin Sulaeman Astri Rinanti Dani Permana Dewi, Intan Lestari Djaenudin Dhaenudin Doni Sugiyana Edward Suhendra Enny Ratnaningsih Enri Damanhuri Enri Damanhuri Ganjar P, Himawan Herto Dwi Ariesyady Herto Dwi Ariesyady, Herto Dwi Himawan G Prabowo Himawan Ganjar P Intan Lestari Dewi Isdiriayani Nurdin Khomaini Hasan Kurniasih Kurniasih Kurniasih Kurniasih Larasati, Amanda Malinda Syifa Yusharani Marisa Handajani Merry Sianipar Mindriany Syafila Muchammad, Amalia Opy Kurniasari Prabowo, Himawan G Prawitasari, Diah Ayu Pujawati Suryatmana Purwanto Purwanto Qomarudin Helmy Ragil Putri Widyastuti Rifka Fadilah Rifka Fadilah, Rifka Sianipar, Merry Sri Harjati Suhardi Sugiyana, Doni Suprihanto Notodarmojo Syarif Hidayat Tri Padmi Tri Padmi Yuni Lisafitri