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All Journal International Journal of Biosciences and Biotechnology Jurnal Pustaka Mitra : Pusat Akses Kajian Mengabdi Terhadap Masyarakat
KHAMDAN KHALIMI
Program studi Agroekoteknologi Fakultas Pertanian Universitas Udayana
Biochemistry, Genetics & Molecular Biology Environmental Science Languange, Linguistic, Communication & Media Library & Information Science Public Health Social Sciences Other

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Harnessing Serratia marcescens: A Dual Role as Biocontrol Agent Against Fusarium oxysporum f.sp. melongenae and Heteroauxin Producer Khalimi, Khamdan; Pranatayana, Ida Bagus Gde; Batin, Charlie B
International Journal of Biosciences and Biotechnology Vol 11 No 2 (2024): International Journal of Biosciences and Biotechnology
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2024.v11.i02.p05

Abstract

Pathogens and plant growth factors significantly influence plant growth and development. This study aims to investigate the potential of Serratia marcescens as a biocontrol agent against Fusarium oxysporum f.sp. melongenae and its capability as a heteroauxin producer. Antagonistic tests of S. marcescens against F. oxysporum f.sp. melongenae were conducted using a dual culture method, while the antifungal activity of S. marcescens extract was assessed through the disk diffusion method. Salkowski's colorimetric test was performed both in vitro and in situ to analyze heteroauxin compounds, further identified by GC-MS. Results demonstrated that all four tested S. marcescens strains could inhibit the growth of F. oxysporum f.sp. melongenae, with inhibition rates ranging from 93.76% to 94.02% compared to the control. These strains produced heteroauxin and its derivatives, including 3-methylindole, salicylic acid, and indole-3-acetic acid methyl ester, as confirmed by GC-MS. This study concludes that the examined S. marcescens strains are promising biocontrol agents and heteroauxin producers, highlighting their potential for sustainable agriculture practices.
Effects Of IAA-Containing Bacterial Application and Dolomite Dosage Reduction on the Growth of Emprit Ginger (Zingiber officinalle Var. Amarum) on Ultisol Soils Leana, Ni Wayan Anik; Ramadhanty, Zahra Avira; Sulistyanto, Prasmadji; Khalimi, Khamdan; Pranatayana, Ida Bagus Gde
International Journal of Biosciences and Biotechnology Vol 11 No 1 (2023): International Journal of Biosciences and Biotechnology
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2023.v11.i01.p02

Abstract

Java ginger, locally known as emprit ginger, is an herb in the rhizome category with many medicinal uses. Indonesia is one of the largest ginger producing countries in the world, however its production in 2019-2022 tends to be unstable. Marginal soil, such as ultisol, can be utilized to increase the Java ginger production. Dolomite and IAA-producing bacteria are some soil ameliorants that can be applied to improve the soil quality. This study aimed to determine the effects of IAA-producing bacterial application and dolomite dosage reduction as well as identify the bacterial isolates and the optimal dose of dolomite in white ginger cultivation on ultisol. The study employed a completely randomized two-factorial design. The observed parameters include plant height (cm), leaf area (cm2), total leaf area (cm2), number of leaves, number of buds, and leaf greenness. Results show that S3 bacterial isolate had significant effects on plant height, leaf area, number of leaves, and leaf greenness. The dolomite dose of 100% showed the best effects on plant height, number of leaves, and number of buds. The interactions between IAA-producing bacteria and dolomite dosage affected plant height, number of leaves, and number of buds.
Hilirisasi Produk Inovasi Pertanian Berbasis Rumput Laut melalui Kolaborasi Akademik dan Industri Pranatayana, Ida Bagus Gde; Wirawan, I Gede Putu; Suada, I Ketut; Yudha, I Kadek Wisma; Khalimi, Khamdan; Phabiola, Trisna Agung
Jurnal Pustaka Mitra (Pusat Akses Kajian Mengabdi Terhadap Masyarakat) Vol 6 No 3 (2026): Jurnal Pustaka Mitra (Pusat Akses Kajian Mengabdi Terhadap Masyarakat)
Publisher : Pustaka Galeri Mandiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55382/jurnalpustakamitra.v6i3.1394

Abstract

Kegiatan pengabdian kepada masyarakat ini dilaksanakan di PT. Alga Nusa Sejahtera, Nusa Lembongan, Bali, dengan tujuan memperkuat hilirisasi inovasi pertanian berbasis rumput laut melalui kolaborasi antara akademisi, industri, dan lembaga pemerintah. Kegiatan dilakukan secara kualitatif-partisipatif melalui observasi lapangan, penyampaian materi, diskusi, pendampingan teknis, serta visitasi laboratorium bersama Balai Besar POM Denpasar. Kegiatan diikuti oleh 18 peserta, dengan 12 peserta dari pihak mitra sebagai sasaran utama evaluasi. Hasil evaluasi menunjukkan adanya peningkatan pemahaman peserta dari skor rata-rata pre-test 2,02 menjadi 3,47 pada post-test, atau meningkat sebesar 71,8%. Peningkatan tertinggi terjadi pada aspek pemahaman GMP/CPPOB sebesar 85,7%, yang menunjukkan pentingnya pendampingan lanjutan pada aspek standar produksi dan keamanan pangan. Selain meningkatkan pemahaman mitra, kegiatan ini juga menghasilkan rekomendasi teknis terkait standardisasi bahan baku, pencatatan produksi, sanitasi, pengendalian mutu, dan kesiapan pemenuhan standar produksi pangan olahan. Hasil kegiatan menunjukkan bahwa kolaborasi akademik–industri dapat menjadi model hilirisasi inovasi berbasis rumput laut yang mendukung peningkatan kapasitas mitra, mutu produk, dan keamanan pangan.
Co-Authors Batin, Charlie B I GEDE PUTU WIRAWAN I Ketut Suada IDA BAGUS GDE PRANATAYANA Leana, Ni Wayan Anik Ramadhanty, Zahra Avira Sulistyanto, Prasmadji TRISNA AGUNG PHABIOLA YUDHA, I KADEK WISMA