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All Journal Jurnal Ilmu Pertanian Indonesia International Journal of Biosciences and Biotechnology
KHAMDAN KHALIMI
Program studi Agroekoteknologi Fakultas Pertanian Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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Harnessing Serratia marcescens: A Dual Role as Biocontrol Agent Against Fusarium oxysporum f.sp. melongenae and Heteroauxin Producer Khalimi, Khamdan; Pranatayana, Ida Bagus Gde; Batin, Charlie B
International Journal of Biosciences and Biotechnology Vol 11 No 2 (2024): International Journal of Biosciences and Biotechnology
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2024.v11.i02.p05

Abstract

Pathogens and plant growth factors significantly influence plant growth and development. This study aims to investigate the potential of Serratia marcescens as a biocontrol agent against Fusarium oxysporum f.sp. melongenae and its capability as a heteroauxin producer. Antagonistic tests of S. marcescens against F. oxysporum f.sp. melongenae were conducted using a dual culture method, while the antifungal activity of S. marcescens extract was assessed through the disk diffusion method. Salkowski's colorimetric test was performed both in vitro and in situ to analyze heteroauxin compounds, further identified by GC-MS. Results demonstrated that all four tested S. marcescens strains could inhibit the growth of F. oxysporum f.sp. melongenae, with inhibition rates ranging from 93.76% to 94.02% compared to the control. These strains produced heteroauxin and its derivatives, including 3-methylindole, salicylic acid, and indole-3-acetic acid methyl ester, as confirmed by GC-MS. This study concludes that the examined S. marcescens strains are promising biocontrol agents and heteroauxin producers, highlighting their potential for sustainable agriculture practices.
Effects Of IAA-Containing Bacterial Application and Dolomite Dosage Reduction on the Growth of Emprit Ginger (Zingiber officinalle Var. Amarum) on Ultisol Soils Leana, Ni Wayan Anik; Ramadhanty, Zahra Avira; Sulistyanto, Prasmadji; Khalimi, Khamdan; Pranatayana, Ida Bagus Gde
International Journal of Biosciences and Biotechnology Vol 11 No 1 (2023): International Journal of Biosciences and Biotechnology
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2023.v11.i01.p02

Abstract

Java ginger, locally known as emprit ginger, is an herb in the rhizome category with many medicinal uses. Indonesia is one of the largest ginger producing countries in the world, however its production in 2019-2022 tends to be unstable. Marginal soil, such as ultisol, can be utilized to increase the Java ginger production. Dolomite and IAA-producing bacteria are some soil ameliorants that can be applied to improve the soil quality. This study aimed to determine the effects of IAA-producing bacterial application and dolomite dosage reduction as well as identify the bacterial isolates and the optimal dose of dolomite in white ginger cultivation on ultisol. The study employed a completely randomized two-factorial design. The observed parameters include plant height (cm), leaf area (cm2), total leaf area (cm2), number of leaves, number of buds, and leaf greenness. Results show that S3 bacterial isolate had significant effects on plant height, leaf area, number of leaves, and leaf greenness. The dolomite dose of 100% showed the best effects on plant height, number of leaves, and number of buds. The interactions between IAA-producing bacteria and dolomite dosage affected plant height, number of leaves, and number of buds.
Antifungal Activity of Gliocladium viride against Fusarium oxysporum Khalimi, Khamdan; Anik Leana, Ni Wayan
Jurnal Ilmu Pertanian Indonesia Vol. 30 No. 2 (2025): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.30.2.268

Abstract

Fusarium oxysporum is a cosmopolitan fungal species that can cause wilt disease in various plants. This study aimed to determine the antifungal activity of Gliocladium viride against F. oxysporum and identify the antifungal compound produced by G. viride. An antagonistic test of G. viride against F. oxysporum was carried out in dual culture. The antifungal activity of G. viride extract on the growth of F. oxysporum was determined using the paper disc diffusion method. The results showed that G. viride inhibited the growth of F. oxysporum colonies, with inhibition percentages ranging from 92.93% to 93.92%. The extract has antifungal activity, with the diameter of the inhibition zone formed being categorized as strong inhibition, which ranges from 19.3 mm to 24.5 mm. G. viride extract contains eleven compounds that have antifungal activity, namely cyclopropanecarboxylic acid; 2-nonanone; 2,3-butanediol; 2-heptanone; acetoin; 2,3-dimethylpyrazine; carbamic acid, phenyl esters; pyridine, 2,3,4,5-tetrahydro; 2-furancarboxaldehyde, 5-methyl; caryophyllene; and 1,2, benzenedicarboxylic acid. The results of this study provide information that G. viride can be used as a biological agent to control wilt in banana, vanilla, tomato and chili plants. Keywords: Gliocladium viride, antifungal activity, Fusarium oxysporum
Co-Authors Anik Leana, Ni Wayan Batin, Charlie B IDA BAGUS GDE PRANATAYANA Leana, Ni Wayan Anik Ramadhanty, Zahra Avira Sulistyanto, Prasmadji