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All Journal Jurnal Ilmu Pertanian Indonesia International Journal of Biosciences and Biotechnology
Anik Leana, Ni Wayan
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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Selection, Isolation, and Identification of Entomopathogenic Bacteria and fungi against Spodoptera frugiperda J.E. Smith in Maize Soesanto, Loekas; Anik Leana, Ni Wayan; Suroto, Agus
International Journal of Biosciences and Biotechnology Vol 11 No 2 (2024): International Journal of Biosciences and Biotechnology
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2024.v11.i02.p01

Abstract

Spodoptera frugiperda is one of the most devastation pests of maize. The control of S. frugiperda so far relies on synthetic pesticides, which can cause deleterious effects on the environments, especially when the insects start to exhibit resistance. The use of entomopathogenic bacteria and/or fungi could provide environmentally friendly alternatives in controlling S. frugiperda. Selections were started by collecting dead S. frugiperda larvae from several locations. These specimens were placed in PDA and NA media. The isolated bacteria and fungi were purified and individually tested on S. frugiperda larvae to determine the rates of mortality and feed consumption. This study employed a complete randomized design and trials were repeated three times. Results showed that Lia and Lib bacterial isolates produce mortality rates of 50% and 23.33%, respectively, on S. frugiperda larvae. Fungal isolates P1, P2, K2, and K3 produced mortality rates on S. frugiperda larvae in the range of 10-16.67%. The highest reduction of feed consumption was produced by K3 isolate at 34.16%. Lia, Lib, P1, P2, and K2 isolates decreased feed consumption at 14.77%, 26.87%, 24.02%, 33.18, and 31.14%, respectively. Molecular identifications showed that Lia and Lib isolates were Aeromonas hydrophila strain DUCC5728HX-3 and Acinetobacter soli strain GFJ2, respectively. This is the first report on entomopathogenic bacteria on S. fruguperda larvae. Fungal isolates K2, K3, P1, and P2 were identified to be Penicillium citrinum strain DUCC5728, Metarhizium rileyi strain 936, and Aspergillus flavus strain KU20018.4, respectively.
Antifungal Activity of Gliocladium viride against Fusarium oxysporum Khalimi, Khamdan; Anik Leana, Ni Wayan
Jurnal Ilmu Pertanian Indonesia Vol. 30 No. 2 (2025): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.30.2.268

Abstract

Fusarium oxysporum is a cosmopolitan fungal species that can cause wilt disease in various plants. This study aimed to determine the antifungal activity of Gliocladium viride against F. oxysporum and identify the antifungal compound produced by G. viride. An antagonistic test of G. viride against F. oxysporum was carried out in dual culture. The antifungal activity of G. viride extract on the growth of F. oxysporum was determined using the paper disc diffusion method. The results showed that G. viride inhibited the growth of F. oxysporum colonies, with inhibition percentages ranging from 92.93% to 93.92%. The extract has antifungal activity, with the diameter of the inhibition zone formed being categorized as strong inhibition, which ranges from 19.3 mm to 24.5 mm. G. viride extract contains eleven compounds that have antifungal activity, namely cyclopropanecarboxylic acid; 2-nonanone; 2,3-butanediol; 2-heptanone; acetoin; 2,3-dimethylpyrazine; carbamic acid, phenyl esters; pyridine, 2,3,4,5-tetrahydro; 2-furancarboxaldehyde, 5-methyl; caryophyllene; and 1,2, benzenedicarboxylic acid. The results of this study provide information that G. viride can be used as a biological agent to control wilt in banana, vanilla, tomato and chili plants. Keywords: Gliocladium viride, antifungal activity, Fusarium oxysporum
Co-Authors KHAMDAN KHALIMI Loekas Soesanto Suroto, Agus