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All Journal International Journal of Biosciences and Biotechnology Jurnal Agro Planta Tropika Jurnal Terapan Abdimas JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Leana, Ni Wayan Anik
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Other

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UTILIZATION OF BETEL LEAVES EXTRACT TO PREVENT THE GROWTH OF Fusarium oxysporum f.sp. capsici 1 CAUSING FUSARIUM WILT DISEASE IN BELL PEPPER Leana, Ni Wayan Anik; Suprapta, Dewa Ngurah
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 21 No. 1 (2021): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12126-33

Abstract

Utilization of betel leaves extract to prevent the growth of Fusarium oxysporum f.sp. capsici causing fusarium wilt diseasein bell pepper. Betel (Piper betle Linn.) is commonly used in Indonesian traditional medicine due to its antimicrobial properties,thus it is often used as an ingredient in natural pesticides. The effectiveness of betel leaf extract as a control for Fusariumoxysporum in several plants showed it’s potential to control the fungi in bell pepper. F. oxysporum f.sp. capsici is one of theimportant pathogens causing Fusarium wilt disease in bell pepper. It is one of the most devastating plant diseases due to itsability to cause a crop failure. The test on the inhibitory capacity of betel leaf extract on the growth of F. oxysporum f.sp.capsici were carried out by growing the fungi on PDA mixed with various concentrations of betel leaf extracts. The resultsshowed that betel leaf extract treatment at 0.02% concentration was able to inhibit the growth of F. oxysporum f.sp. capsici inPDA at 95.54%. The full inhibition of colony growth (100%) was achieved in the extract treated with the concentration of0.03%. Meanwhile treatment of 0.17% betel leaf extract was able to inhibit the growth of F. oxysporum f.sp. capsici on bellpepper stems. Following this result, fractionation of the betel leaf extract by column chromatography was conducted, resultingin 44 fractions. The bioassays of those fractions showed that, there were seven fractions that reveal inhibition capabilityagainst F. oxysporum f.sp. capsici.
Application of Bio P60 and Bio T10 alone or in combination to control Fusarium wilt of Hydroponic Melon Soesanto, Loekas; Nuraini, Ika Vidi; Sastyawan, Murti Wisnu Ragil; Mugiastuti, Endang; Leana, Ni Wayan Anik; Rahayuniati, Ruth Feti
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 24 No. 2 (2024): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.224199-211

Abstract

The research aimed to determine the effect of single and combined applications of Bio P60 and Bio T10 in suppressing stem base rot and its effect on the growth and production of hydroponic melon. This research was conducted at Flos Hydroponic Organic at Bansari Village, Bansari District, Temanggung Regency from February to June 2023. Randomized Block Design was used with 6 replicates. The treatments were control (propamocarb hydrochloride), Bio P60, Bio T10, and a combination of Bio P60 and Bio T10 (1:1, v/v). Variables observed were incubation period, disease intensity, infection rate, area under disease progress curve (AUDPC), control effectiveness, plant length, number of leaves, fresh weight, leaf color, first flowering date, first fruit formation, number of fruits per plant, fruit weight per plant, and phenolic compounds qualitatively. The results showed that the combined treatment of Bio P60 and Bio T10 had the best effect indicated by delaying the incubation period, reducing disease intensity, reducing infection rates, reducing AUDPC values, increasing the value of control effectiveness, increasing plant length, number of leaves, plant fresh weight, leaf color, time of first flower appearance, time of fruiting, and fruit weight respectively of 31.25, 41.19, 13.33, 65.31, 55.61, 17.25, 5.57, 36.44, 11.47, 8.55, 9.63, and 22.92 % compared to control. The application of Bio P60, Bio T10, and the combination could increase the phenolic compounds (tannins, saponins, and glycosides) qualitatively in melon leaves.
Effects Of IAA-Containing Bacterial Application and Dolomite Dosage Reduction on the Growth of Emprit Ginger (Zingiber officinalle Var. Amarum) on Ultisol Soils Leana, Ni Wayan Anik; Ramadhanty, Zahra Avira; Sulistyanto, Prasmadji; Khalimi, Khamdan; Pranatayana, Ida Bagus Gde
International Journal of Biosciences and Biotechnology Vol 11 No 1 (2023): International Journal of Biosciences and Biotechnology
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2023.v11.i01.p02

Abstract

Java ginger, locally known as emprit ginger, is an herb in the rhizome category with many medicinal uses. Indonesia is one of the largest ginger producing countries in the world, however its production in 2019-2022 tends to be unstable. Marginal soil, such as ultisol, can be utilized to increase the Java ginger production. Dolomite and IAA-producing bacteria are some soil ameliorants that can be applied to improve the soil quality. This study aimed to determine the effects of IAA-producing bacterial application and dolomite dosage reduction as well as identify the bacterial isolates and the optimal dose of dolomite in white ginger cultivation on ultisol. The study employed a completely randomized two-factorial design. The observed parameters include plant height (cm), leaf area (cm2), total leaf area (cm2), number of leaves, number of buds, and leaf greenness. Results show that S3 bacterial isolate had significant effects on plant height, leaf area, number of leaves, and leaf greenness. The dolomite dose of 100% showed the best effects on plant height, number of leaves, and number of buds. The interactions between IAA-producing bacteria and dolomite dosage affected plant height, number of leaves, and number of buds.
BIMBINGAN TEKNIS PEMBUATAN PESTISIDA ORGANIK UNTUK MENGENDALIKAN PENYAKIT TANAMAN SAYUR DATARAN TINGGI RAMAH LINGKUNGAN Sumarni, Eni; Soesanto, Loekas; Herliana, Okti; Leana, Ni Wayan Anik; Zulkifli, Lutfi; Priswanto, Priswanto; Purnomo, Widhiatmoko Herry
Jurnal Terapan Abdimas Vol 9, No 1 (2024)
Publisher : UNIVERSITAS PGRI MADIUN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25273/jta.v9i1.14563

Abstract

Abstract. Secondary metabolites are organic compounds by-products of organisms metabolism. The use of antagonistic microbial secondary metabolites has been applied and can control several plant diseases. However, the socialization of antagonistic microbial secondary metabolites and their production among farmers has not been carried out. The aim of the activity was to increase farmers' knowledge about safe and environmentally friendly control of vegetable plant diseases, farmers' skills in effective, efficient, environmentally friendly plant disease control, the quantity and quality of crop production, and farmers' income through increasing farming efficiency. The target of this activity is the Rahayu Makmur Farmer Group Association (Gapoktan), Bansari District, Temanggung Regency. Gapoktan Rahayu Makmur, which consists of 21 farmer groups, is a highland horticultural crop farmer. Transfer of environmentally friendly plant disease management technology is carried out through education in the form of providing materials and discussions and practice. Technical guidance activities are able to increase farmers' knowledge regarding the manufacture of organic pesticides and the independence of farmers to produce organic pesticides. This is evidenced by the follow-up of two farmer groups who practice the manufacture of organic pesticides after the guidance. Abstrak. Metabolit sekunder merupakan senyawa organik hasil samping dari metabolisme organisme. Metabolit sekunder mikroba antagonis sudah diaplikasikan dan dapat mengendalikan beberapa penyakit tanaman. Akan tetapi, sosialisasi tentang metabolit sekunder mikroba antagonis dan pembuatannya di kalangan petani belum dilakukan, sehingga perlu diadakan bimbingan teknis (Bimtek) pembuatan pestisida organik berbasis metabolit sekunder mikroba antagonis untuk mengendalikan penyakit tanaman. Tujuan kegiatan adalah meningkatkan pengetahuan petani tentang pengendalian penyakit tanaman sayur aman dan ramah lingkungan, ketrampilan petani tentang pengendalian penyakit tanaman yang efektif, efisien, ramah lingkungan, kuantitas dan kualitas produksi tanaman, serta pendapatan petani melalui peningkatan efisiensi usahatani. Sasaran kegiatan adalah Gabungan Kelompok Tani (Gapoktan) Rahayu Makmur, Kecamatan Bansari, Kabupaten Temanggung. Gapoktan Rahayu Makmur yang terdiri dari 21 kelompok tani merupakan petani tanaman hortikultura dataran tinggi. Transfer teknologi pengelolaan penyakit tanaman ramah lingkungan dilakukan melalui pendidikan berupa pemberian materi dan diskusi serta praktik pembuatan. Kegiatan Bimtek mampu meningkatkan pengetahuan petani mengenai pembuatan pestisida organik. Selain peningkatan pengetahuan petani, Bimtek juga meningkatkan kemandirian petani untuk memproduksi pestisida organik. Hal ini dibuktikan dengan adanya tindak lanjut dari dua kelompok tani yang mempraktekkan pembuatan pestisida organik berbasis metabolit sekunder pasca Bimtek. Pestisida organik berbasis metabolit sekunder tersebut mereka gunakan untuk mengendalikan hama penyakit pada tanaman yang dibudidayakan.
Seed Bio-Priming to Enhance Seed Germination and Seed Vigor of Rice Using Rhizobacteria from The Northern Coast of Pemalang, Central Java, Indonesia Purwanto, Purwanto; Oktaviani, Eka; Leana, Ni Wayan Anik
PLANTA TROPIKA Vol 10, No 2 (2022)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v10i2.13722

Abstract

The growth and yield of plants are strongly influenced by the early growth ability of the plants. Similar germination and good seed vigor will greatly support plant growth and increase production. Increasing the germination and vigor of seeds can be done through biopriming. The application of biopriming using rhizobacteria is developing environmentally friendly agricultural technology. This study aimed to determine the effect of inoculation of rhizobacteria from the north coast of Pemalang on rice plants' germination and vigor index. The study was arranged in a Randomized Block Design, consisting of 10 treatments with three replications. Ten rhizobacteria isolates were isolated from the North Coast of Pemalang, Central Java, consisting of Ju1, Jn3, Jn1, J, J12, J5, Kn1, A3, Jn, and K3. The biopriming with rhizobacteria isolated from the rice rhizosphere of the Northern Coast of Pemalang increased the seed germination rate, seed vigor index, and early vegetative growth of rice seedlings. Inoculation with isolate J12 produced the highest vigor index of 8280.01. The results of this study imply that the application of rhizobacteria from saline soil has the potential to increase the vigor of rice seedlings to impact better seedling growth in saline conditions.
Induksi ketahanan tanaman padi terhadap serangan pathogen busuk pelepah (Rhizoctonia solani) menggunakan halotoleran bakteri Diazotrof asal pantai utara Pemalang, Jawa Tengah Isnaeni, Fenty Chakimatul; mugiastuti, Endang; Leana, Ni Wayan Anik; Oktaviani, Eka; Purwanto, Purwanto
Jurnal AGRO Vol 9, No 1 (2022)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/18516

Abstract

Padi merupakan komoditas pangan yang memiliki peranan terpenting dalam memenuhi kebutuhan pokok masyarakat Indonesia. Ekstensifikasi produksi padi dapat dilakukan dengan memanfaatkan lahan marjinal seperti lahan salin. Lahan salin merupakan lahan yang memiliki kadar kadar garam tinggi akibat intrusi air laut maupun tingginya laju evaporasi. Pengembangan budidaya padi di lahan salin memiliki kendala berupa serangan patogen busuk pelepah (Rhizoctonia solani). Alternatif pengendalian patogen selain menggunakan pestsida kimia, dapat dilakukan menggunakan Plant Growth Promoting Rhizobacteria (PGPR), seperti kelompok bakteri diazotrof. Penelitian ini bertujuan untuk mengetahui potensi bakteri diazotrof lahan salin dalam meningkatkan ketahanan tanaman padi serta kemampuannya dalam memacu pertumbuhan padi yang terinfeksi R. solani. Penelitian dilaksanakan di Experimental Farm dan Laboratorium Agronomi & Hortikultura, Fakultas Pertanian Unsoed pada bulan Oktober 2021 - Februari 2022. Rancangan yang digunakan adalah Rancangan Acak Kelompok Lengkap (RAKL) dengan 11 perlakuan dan diulang tiga kali, meliputi kontrol tanpa inokulasi bakteri diazotrof dan inokulasi isolat Ju1, Jn3, Jn1, J, J12, J5, Kn1, A3, Jn dan K3. Semua tanaman juga dinokulasi dengan Rhizoctonia solani. Hasil penelitian menunjukkan bahwa inokulasi bakteri diazotrof dapat meningkatkan ketahanan padi yang terinfeksi jamur R. solani, ditandai dengan penurunan intensitas penyakit hingga 70%, peningkatan kandungan saponin, tanin dan hidrokuinon, serta peningkatkan pertumbuhan tinggi tanaman dan panjang akar total padi.ABSTRACTRice is the most important  food commodity that supply the basic needs of the Indonesian people. The development of rice cultivation in salin land has obstacles in the form of attacks by sheat blight pathogen (Rhizoctonia solani). Alternative to controlling pathogens other than using chemical pesticides is by using Plant Growth Promoting Rhizobacteria (PGPR), such as diazotroph bacteria. This research aimed to determine the potential of diazotrof bacteria in increasing rice resistance and its ability to stimulate the growth of rice infected with R. solani. The research was carried out at Experimental Farm and Agrohorti Laboratory, Faculty of Agriculture Unsoed in October 2021 - February 2022. The design used was Randomized Completely Block Design (RCBD) with 11 treatments and repeated three times, including controls without inoculation of diazotroph bacteria and inoculation of isolates Ju1, Jn3, Jn1, J, J12, J5, Kn1, A3, Jn and K3. All plants were also inoculated with R. solani. The results showed that inoculation with diazotrof bacteria could increase the rice resistance towards attack of sheath blight pathogen (R. solani) that characterized by a decrease disease intensity up to 70%, increase saponins, tannins and hydroquinones content, also increase growth of plant height and total root length of rice.
Seed Bio-Priming to Enhance Seed Germination and Seed Vigor of Rice Using Rhizobacteria from The Northern Coast of Pemalang, Central Java, Indonesia Purwanto, Purwanto; Oktaviani, Eka; Leana, Ni Wayan Anik
PLANTA TROPIKA Vol. 10 No. 2 (2022)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v10i2.13722

Abstract

The growth and yield of plants are strongly influenced by the early growth ability of the plants. Similar germination and good seed vigor will greatly support plant growth and increase production. Increasing the germination and vigor of seeds can be done through biopriming. The application of biopriming using rhizobacteria is developing environmentally friendly agricultural technology. This study aimed to determine the effect of inoculation of rhizobacteria from the north coast of Pemalang on rice plants' germination and vigor index. The study was arranged in a Randomized Block Design, consisting of 10 treatments with three replications. Ten rhizobacteria isolates were isolated from the North Coast of Pemalang, Central Java, consisting of Ju1, Jn3, Jn1, J, J12, J5, Kn1, A3, Jn, and K3. The biopriming with rhizobacteria isolated from the rice rhizosphere of the Northern Coast of Pemalang increased the seed germination rate, seed vigor index, and early vegetative growth of rice seedlings. Inoculation with isolate J12 produced the highest vigor index of 8280.01. The results of this study imply that the application of rhizobacteria from saline soil has the potential to increase the vigor of rice seedlings to impact better seedling growth in saline conditions.
Application of biocontrol products Bio P60 and Bio T10 as single or in combination in suppressing chili fruit anthracnose in the field Mugiastuti, Endang; Hidayat, Fitrian; Sastyawan, Murti Wisnu Ragil; Leana, Ni Wayan Anik; Soesanto, Loekas
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 25 No. 2 (2025): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.225230-240

Abstract

This study aimed to evaluate the effectiveness of single or combined applications of Bio P60 and Bio T10 products in suppressing anthracnose disease in chili pepper under field conditions. The research was conducted at an altitude of 1200 m above sea level using a randomized block design with five treatments: control, chemical fungicide, Bio P60, Bio T10, and a combination of Bio P60 and Bio T10, each replicated five times. Observed variables included incubation period, disease incidence, disease intensity, area under disease progress curve (AUDPC), infection rate, plant height, number of leaves, time of first flower, time of first fruit, number of fruits, fruit weight per plant, harvest weight per plot, and qualitative phenolic compound content. The results showed that the combined application of Bio P60 and Bio T10 was the most effective, delaying the incubation period, suppressing disease intensity, and reducing AUDPC by 13.71%, 69.34%, and 47.06%, respectively, compared to the control. The combination treatment also enhanced plant growth and yield, increasing plant height, number of fruits, fruit weight per plant, and harvest weight per plot by 27.38%, 62.65%, 90.85%, and 82.99%, respectively. Furthermore, the application of Bio P60, Bio T10, and their combination increased phenolic compound content qualitatively in chili pepper plants.
Co-Authors Dewa Ngurah Suprapta Eka Oktaviani Eka Oktaviani Endang Mugiastuti Eni Sumarni Hidayat, Fitrian IDA BAGUS GDE PRANATAYANA Isnaeni, Fenty Chakimatul KHAMDAN KHALIMI Loekas Soesanto Nuraini, Ika Vidi Okti Herliana, Okti Priswanto Priswanto, Priswanto Purnomo, Widhiatmoko Herry Purwanto Purwanto Purwanto, Purwanto Ramadhanty, Zahra Avira Ruth Feti Rahayuniati Sastyawan, Murti Wisnu Ragil Sulistyanto, Prasmadji Zulkifli, Lutfi