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Identification and Phylogenetic Analysis of Bacterial Isolates from Litopenaeus vannamei Shrimp Culture System and Gut Environment Based on 16SrRNA Gene Sequence Data TUBAGUS HAERU RAHAYU; INDRAWATI GANDJAR; ETTY RIANI; IIN SITI DJUNAIDAH; WELLYZAR SJAMSURIDZAL
Microbiology Indonesia Vol. 3 No. 2 (2009): August 2009
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (85.506 KB) | DOI: 10.5454/mi.3.2.3

Abstract

Selected bacterial isolates from a Litopenaeus vannamei shrimp culture system and gut environment were assessed using 16S rRNA gene sequencing method to identify their identity and to construct their phylogenetic relationship. In a preliminary study, a total of 19 isolates were selected as probiotics. These isolates were prepared using freeze and heat-shock method to obtain the DNA template. PCR amplification of 16S ribosomal RNA gene of isolates was carried out using bacterial universal primers 9F and 1510R and was sequenced using an automated DNA sequencer. These gene sequences were compared with other gene sequences in the GenBank database (NCBI) using a BLAST search to find closely related sequences. Alignment of these sequences with sequences available from GenBank database was carried out to construct a phylogenetic tree for these bacteria. Most of the isolates obtained, i.e. 17 out of the 19 isolates, belonged to different species of Bacillus, sharing 95 to 99% 16S ribosomal RNA identity with the respective type-strain, whereas the remaining 2 isolates belonged to Micrococcus sp. and Micrococcus luteus with 97 to 99% 16S rRNA homology, consecutively.
Characteristics of seaweed caraginan Kappaphycus alvarezii on cultivation system with different seed weight Rasnijal, Muhammad; Kurniaji, Ardana; Anton, Anton; Budiyati, Budiyati; Putri Renitasari, Diana; Suhermanto, Achmad; Mulyono, Mugi; Djunaidah, Iin Siti; Rahardjo, Sinung; Sektiana, Sinar Pagi; Ridwan, Ridwan
Jurnal Akuakultur Indonesia Vol. 23 No. 1 (2024): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.23.1.71-78

Abstract

Seaweed (Kappaphycus alvarezii) could be a source of carrageenan needed for industrial sector. The purpose of this study to analyze the characteristics of carrageenan seaweed cultivated with bag and non-bag cultivation systems and different seed weights. The research was conducted in the waters of Bone Bay and the Laboratory of the Indonesian Center for Brackish Water Cultivation and Fisheries Extension in Maros. This study used a factorial completely randomized design (CRD) consisting of factor A (using bags and non-bags), and factor B (seed weight 15, 50, 75, 100, and 125 g). The results showed that the cultivation method factor, seed weight factor, and their interaction had an effect on carrageenan yield, gel strength, ash content and water content (P <0.05). Different cultivation systems with different initial seed weight combinations showed different best results for each parameter. The highest value of yield of carrageenan was observed in the bag culture system with a seed weight of 100 g, namely 29.18 ± 1.10%. The highest value of gel strength was observed in the non-bagged cultivation system with a seed weight of 75 g, namely 1344.69 ± 18.43 g/cm2. The highest value of ash content was found in the non-bagged cultivation system with 125 g of seed weight, namely 30.02 ± 0.13%. The highest value of water content was found in the bag culture system at a seed weight of 15 g, namely 38.63 ± 0.26%. Different cultivation methods and seed weight resulted in other carrageenan characteristics of seaweed for each parameter. Keywords: gel strength, ash content, moisture content, yield ABSTRAK Rumput laut (Kappaphycus alvarezii) memiliki potensi sebagai sumber karaginan yang banyak dibutuhkan untuk bidang industri. Penelitian ini bertujuan untuk menganalisis karakteristik karaginan rumput laut yang dibudidayakan dengan sistem budidaya kantong dan non kantong serta berat bibit yang berbeda. Penelitian dilaksanakan di perairan Teluk Bone dan Laboratorium Balai Riset Budidaya Air Payau dan Penyuluhan Peikanan Maros. Percobaan penelitian dilakukan melalui rancangan acak lengkap (RAL) faktorial yang terdiri faktor A (memakai kantong dan non kantong), faktor B (berat bibit 15, 50, 75, 100, dan 125 g). Hasil penelitian menunjukkan bahwa faktor metode budidaya, berat bibit dan interaksi keduanya berpengaruh terhadap rendeman karaginan, kekuatan gel, kadar air, kadar abu (P<0,05). Penggunaan sistem budidaya yang berbeda dengan kombinasi berat awal bibit berbeda menunjukkan hasil terbaik yang tidak sama pada tiap parameter. Nilai tertinggi rendeman keraginan teramati pada sistem budidaya kantong dengan berat bibit 100 g yakni 29,18 ± 1,10 %. Nilai tertinggi kekuatan gel teramati pada sistem budidaya non kantong dengan berat bibit 50 g yaitu 1344,69 ± 18,43 g/cm2. Nilai kadar abu tertinggi pada sistem budidaya non kantong dengan berat bibit 125 g yaitu 30,02 ± 0,13%. Nilai kadar air tertinggi pada sistem budidaya kantong dengan berat bibit 15 g yaitu 38,63 ± 0,26 %. Penggunaan metode budidaya dan berat bibit yang berbeda menghasilkan karakteristik karaginan rumput laut yang berbeda pada tiap parameter. Kata kunci: kekuatan gel, kadar abu, kadar air, rendemen