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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA Media Abdimas SENAPAS
Kianto Atmodjo
Biology Study Program Faculty Of Biotechnology Universitas Atma Jaya Yogyakarta
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Environmental Science Health Professions Industrial & Manufacturing Engineering Library & Information Science Public Health Social Sciences Other

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Journal : SENAPAS

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The Secondary Metabolite and Antibiotic Activity of Calabash Leaf and Fruit (Crescentia Cujete L) Ethanol Extract Kianto Atmodjo; Boy Rahardja Sidharta
Seminar Nasional Penelitian dan Abdimas Vol 1 No 1 (2023): Juni
Publisher : Lembaga Penelitian dan Pengabdian pada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/senapas.v1i1.7395

Abstract

Antibiotic resistance is a big problem in medication, food security, and human development goals. Indonesia has a very huge diversity in term of medicinal plants, which are proven to cure many illnesses by their ancestors, including Calabash. However, research on the bioactive substances from the leaves and fruit of calabash as antibiotic [both antibacterial and antifungal] are dearth. Antibiotic activity test of the substances found has never been done yet, including the comparison to antibiotic ampicillin. The objectives of the research are: a] to determine bioactive substances found in the leaves and fruit of Calabash which have antibiotic activities, b] to reveal the antibiotic activity of leaves and fruit extract of calabash to two [2]] pathogenic bacteria and two [2] pathogenic fungus, c] to determine minimum inhibitory concentration [MIC] of calabash’s leaves and fruit extract to four [4] pathogenic microbes. The methods applied in this research are as follows. The bioactive substances were extracted using maceration method with ethanol as the solvent. The extracts were then analyzed using GC-MS equipment and tested to four [4] pathogenic microbes in agar diffusion method to determine the inhibition zones of the extracts. Later, the extract was also used to determine the MIC of the extracts by dilution technique. This present research found that fruit and leaves extract have many bioactive substances such as saponin, flavonoid, terpenoid and alkaloid that have low-medium antibacterial and antifungal activity, with minimum concentration of inhibition 50% for bacteria and 75% for fungus., which later can be developed into products or antibacterial and antifungal drugs that may give benefits to Indonesian people and the world.
Daya Antibakteri Minyak Atsiri Daun Sirih Hijau (Piper betle L.) dan Sirih Merah (Piper Crocatum) Terhadap Bakteri Pseudomonas aeruginosa dan Staphylococcus aureus Lidwina Ella Septiani; Kianto Atmodjo; B. Boy Rahardjo Sidharta
Seminar Nasional Penelitian dan Abdimas Vol 2 No 1 (2024): Juni
Publisher : Lembaga Penelitian dan Pengabdian pada Masyarakat

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Abstract

Abstract —Betel, have long been known as antibacterial. Based on the shape of the leaves, taste and aroma, betel is divided into several types, but the most widely used are green and red betel leaves. Green and red betel leaves contain phytochemicals such as essential oils compounds such as kavikol, cineol and eugenol. The purpose of this study was to determine the differences in the content of green and red betel leaf essential oil in the old and young treatments and its effectiveness at the concentration used against Staphylococcus aureus and Pseudomonas aeruginosa bacteria. This research begins with the preparation of betel leaf for essential oil distillation, isolation of essential oils by steam-water distillation, analysis of components or compounds of essential oils using GCMS, preparation of pure essential oil stock solutions (100%), preparation of Staphylococcus aureus and Pseudomonas aeruginosa bacteria. then identification test was carried out with several test methods, antibacterial activity testing which began with making variations in concentrations of 10%, 15%, 20%, 25% and 30% with the addition of a positive control of ampicillin disk and a negative control of DMSO with the well diffusion method which was incubated for 24 hours. hours at 37°C. The results of the measurement of the inhibitory zone were analyzed by ANOVA using the factorial RAL pattern, followed by the measurement of the minimum inhibitory concentration (MIC) with a concentration variation of 5%, 10%, 20% and 30% and the determination of the minimum inhibitory concentration (MIC). The results showed that dark green betel leaf essential oil had better antibacterial activity in the inhibition zone test with a concentration of 30% against both bacteria with an inhibitory zone area of ​​1.88 cm for Staphylococcus aureus and 1.21 cm for Psedumonas aeruginosa and the minimum inhibitory concentration test was able to inhibit at a concentration of 10% 10% against Staphylococcus aureus bacteria and at a concentration of 5% on Psedumonas aeruginosa bacteria.
Co-Authors B. Boy Rahardjo Sidharta Benediktus Yudo Leksono Boy Rahardja Sidharta Boy Rahardjo Sidharta Dhany Krisna Exsyupransia Mursyanti Ines Septi Arsiningtyas Lidwina Ella Septiani To'bungan, Nelsiani