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All Journal Chemica: Jurnal Ilmiah Kimia dan Pendidikan Kimia Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati BIOSFER : Jurnal Biologi dan Pendidikan Biologi Indonesian Journal of Islamic History and Culture Indonesian Journal of Fundamental Sciences Jurnal Ilmiah Manuntung: Sains Farmasi dan Kesehatan Bionature
Alimuddin Ali
Unknown Affiliation
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Education Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences

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Purifikasi dan Karakterisasi Krom Reduktase Bacillus sp LKA9 Alimuddin Ali; Endang Sutariningsih Soetarto; J. Sri Widada
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 2 (2006): June 2006
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v11i2.2625

Abstract

Chromate reductase is one of the potential enzymes for hexavalent chrom detoxification. Most of the enzyme is produced by bacteria, especially Bacillus. The aim of this research was to study chromate reductase activity isolated from Bacillus sp LKA9. Bacillus sp LKA9 was isolated from leather tannery liquid waste and used as a model in the experiment. Bacillus sp LKA9 was isolated through enrichment culture using Salt Base Solution containing 3 mM K2CrO4. Chromate reductase was isolated from bacteria by growing on a liquid medium containing chrom hexavalen (Cr VI) through several steps. The first step of the isolation process was to use the precipitated process using ammonium sulphate (30-80%). The next step, crude enzymes from the first step was partially purified through DEAE-Cellulose of Ion Exchange Chromatography Column. Diphenylcarbazide methods was used to examine the activity of enzyme fractions. The result of the experiment revealed that all protein could be precipitated by ammonium sulphate, and the cytoplasmic fraction at saturation of 50-70% showed high enzyme activity. Purified enzymes showed an increase activity 69,385 times to that of crude enzymes. The enzyme optimal had temperature and pH were 350C and 5; respectively. KM of enzyme was 0,0075 mM, and Vmax was 2500 mol/minute/mg protein. Enzyme activity was not inhibited by Cu2+, but an ion Ag2+ and Hg2+ inhibited the enzyme activity un-competitive. The activity of enzyme was demonstrated on SDS-PAGE by appearing typically band with molecular weight 29,26 kDa, it was assumed as chromate reductase.
Skrining Senyawa Bioaktif pada Tumbuhan Mangrove yang Dapat Menghambat Pertumbuhan Bakteri Penyebab Penyakit Red Spot Disease Darminto, ,; Alimuddin Ali; Iwan Dini
Chemica: Jurnal Ilmiah Kimia dan Pendidikan Kimia Vol 12, No 1 (2011)
Publisher : Jurusan Kimia FMIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (219.643 KB) | DOI: 10.35580/chemica.v12i1.137

Abstract

ABSTRAKTelah dilakukan penelitian mengenai skrining senyawa bioaktif dari tumbuhan mangrove yang dapat menghambat pertumbuhan bakteri MAS. Penelitian dilakukan dengan metode skrining senyawa aktif dengan menggunakan kolom kromatografi dan pengujian antibakteri dengan metode difusi agar. Uji IC50 dilakukan dengan menggunakan sel vero yang dilajutkan dengan uji mortalitas ikan uji. Hasil penelitian menunjukkan bahwa semua komponen bioaktif dari Avicennia sp memiliki nilai IC50 > 200µg/ml, viabilitas ikan uji lebih tinggi dengan konsentrasi senyawa 15-25g/Kg pakan dan berbeda nyata dengan 1-10g/Kg pakan pada Uji BNT1%. Kata kunci: Senyawa Bioaktif, Mangrove, Red Spot Disease ABSTRACT Screening of bioactive compounds from mangrove plants that can inhibit bacterial growth MAS used column chromatography and the antibacterial test by agar diffusion method. IC50 test by vero cells that continued by fish mortality test. The results showed that all the bioactive components of Avicennia sp have IC50 values ​​> 200μg/ml, the viability of fish with higher concentrations of test compound 15-25g/Kg significantly different from the feed on the test 1-10g/Kg BNT1%. Key words: Bioactive compounds, Mangrove, Red Spot Disease
Analisis Kebutuhan Pengembangan Modul Berbasis Hots pada Materi Biologi Kelas XI Semester Genap SMA/MA di Sulawesi Selatan Adnan; Alimuddin Ali; Dwi Rahayu Dini
Biosfer : Jurnal Biologi dan Pendidikan Biologi Vol. 7 No. 2 (2022): BIOSFER: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP Unpas,

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23969/biosfer.v7i2.6502

Abstract

Integrasi keterampilan abad ke-21 pada proses pembelajaran menjadi salah satu langkah pemerintah untuk mendorong kemampuan peserta didik pengembangan terkait teknologi yang semakin pesat serta sebagai salah satu penerapan dalam masyarakat. penelitian ini dilakukan untuk mendeskripsikan kebutuhan pengembangan modul berbasis HOTS, dengan harapan bahwa bahan ajar mampu mengaktifkan peserta didik dalam proses pembelajaran, pengembangan konsep, berpikir kritis, dan memecahkan masalah yang secara tidak langsung mengarah pada tingkat kognitif tertinggi. Jenis penelitian yang digunakan adalah penelitian deskriptif. Pengumpulan data dilakukan dengan instrumen angket analisis kebutuhan modul. Hasil analisis dokumen yang didapatkan penggunaan dimensi kognitif LOST dan HOTS masih tidak seimbang,
EFEK AIR REBUSAN KAYU CINA (Lannea coromandelica) TERHADAP HISTOPATOLOGI MUKOSA LAMBUNG MENCIT (Mus musculus) YANG DIINDUKSI ASAM KLORIDA (HCl) Andi Mu'nisa; Alimuddin Ali; M. Junda; Andi Muflihunna; Nur Istiqamah
Indonesian Journal of Fundamental Sciences Vol 5, No 1 (2019)
Publisher : Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (339.74 KB) | DOI: 10.26858/ijfs.v5i1.9370

Abstract

Penelitian ini bertujuan untuk mengetahui efek air rebusan kayu cina terhadap histopatologi mukosa lambung mencit (mus musculus) yang diinduksi asam klorida (HCl). Penelitian ini menggunakan mencit jantan strain ICR sebanyak 45 ekor yang dibagi dalam 3 kelompok perlakuan, yaitu kelompok mencit normal, kelompok mencit gastritis, dan kelompok mencit dengan pemberian air rebusan kayu cina selama 7 dan 14 hari sebanyak 0.3 cc. Hasilnya menunjukkan bahwa setelah pemberian air rebusan kayu cina ada perbaikan sel-sel mukosa lambung mencit. Kesimpulan dalam penelitian ini adalah bahwa air rebusan kayu cina menunjukkan adanya perbaikan secara histopatologis mukosa lambung mencit yang diinduksi asam klorida (HCl).
ISLAMIC EDUCATION THOUGHTS OF KH. AHMAD DAHLAN AT THE YAMISA ISLAMIC BOARDING SCHOOL IN 1968 Alimuddin Ali
Indonesian Journal of Islamic History and Culture Vol 4 No 1 (2023): Indonesian Journal of Islamic History and Culture
Publisher : The Department of Islamic History and Culture in cooperation with the Center for Islamic History and Culture in Aceh and Malay World Studies (PUSAKA), Universitas Islam Negeri Ar-Raniry Banda Aceh, Aceh, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ijihc.v4i1.1449

Abstract

Islamic education is an educational system based on the Qur'an and Sunnah. Kh. Ahmad Dahlan in his thoughts on Islamic education based on the Qur'an and Sunnah. This must be accompanied by other sciences or what is commonly referred to as other general sciences in order to create individuals who are rich in knowledge outside of religious knowledge. The education system at the Yamisa Islamic Boarding School, despite applying the teachings of the Qodariyah Naqsabandiyah order. But in practice in line with Kh. Ahmad Dahlan in which the Yamisa Islamic Boarding School teaches its students about religion and includes other knowledge so that the students have broad knowledge. The methodology used in making this article is to use historical research methods which consist of several stages, namely heuristics, criticism, interpretation and historiography. The research method in this article uses the historical method. The first source collection was to find information and collect data from a book entitled Kh. Ahmad Dahlan (1868-1923), Rattan the Kiai: The Life Story of Mama Uyeh. Sources from the internet such as e-books, journals entitled understanding of education, Islamic Education Thought Perspective Kh. Ahmad Dahlan (1869-1923) and Kh. Hasyim Asy'ari 1871-1947(M): Comparative study in the concept of reforming Islamic education in Indonesia. and other articles such as the thesis entitled Islamic Education Thought According to Kh. Ahmad Dahlan. As well as interviews conducted with Mr. Heri Haris Mawardi as the administrator of the Yamisa boarding school. Islamic Education KH. Ahmad Dahlan at Yamisa Islamic Boarding School, although in practice there are differences in his flow because of NU and Muhammadiyah. However, in terms of the education system, it is not much different, because the thought of Islamic education at the Yamisa pesantren also applies the same thing as KH. Ahmad Dahlan in his education system. Namely, Yamisa Islamic Boarding School also implements a non-formal education system so that the education system can keep up with the times with openness to general knowledge so that students gain knowledge related to religion and the public.
PENGARUH VARIASI KONDISI FERMENTASI TERHADAP PRODUKSI METABOLIT ANTIBAKTERI EKSTRAK ISOLAT I5 FUNGI ENDOFIT Anredera cordifolia (Ten.) Steenis Ismail Ismail; Megawati Megawati; Alimuddin Ali; Fuji Asmiati Ningsih
Jurnal Ilmiah Manuntung Vol 5 No 2 (2019): Jurnal Ilmiah Manuntung
Publisher : jurnal ilmiah manuntung sekolah tinggi ilmu kesehatan samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51352/jim.v5i2.260

Abstract

Endophytic fungi are one of the microorganisms that live in plants and symbiotic with their host plant produce secondary metabolites. This study aims to determine the optimum growth profile of endophytic fungus isolated from the leaf of Anredera cordifolia (Ten.) Steenis namely isolate I5 as antibacterial. The fungus growth profile optimization analyzed by a correlation between fermentation time and mycelium weight, which collected every 24 hours for 15 days. The media were varied at pH conditions of 4, 7, and 8 in each PDYB medium (glucose and fructose substrates). The results showed that isolate I5 grew optimally on the 13th day on the PDYB medium pH 8. Ethyl acetate extract 5% of isolate I5 could inhibit bacteria growth with the largest inhibition zone diameter against Staphylococcus aureus of 9.96 mm (± 0.60) and Escherichia coli of 9.03 mm (± 0.46).
Potensi Ekstrak Etanol Kulit Batang Tumbuhan Mangrove (Avicennia spp.) dalam Menghambat Pertumbuhan Bakteri Aeromonas hydrophyla Darminto; Alimuddin Ali; Iwan Dini
Bionature Vol. 10 No. 2 (2009): Oktober 2009
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The aim of this research is to show the potential of tree bark of mangrove (Avicennia sp.) plant asantibacterial to against Aeromonas hydrophyla. This bacterium causes fishery cultivation disease e.g. theMAS (Motile Aeromonads Septicemia). Antibacterial activity test was done using paper diffusion and dilution methods. The research result showed that extract of tree bark of mangrove (Avicennia sp.) plant having potency as antibacterial particularly to the A. hydrophyla.
Evaluation of Mango Leaf Extract as a Natural Preservative for Enhancing the Shelf Life and Quality of Milkfish Nurhayati B; Yahdiel Ronrong; Nani Kurnia; Alimuddin Ali
Bionature Vol. 26 No. 1 (2025): April-Juni 2025
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v26i1.7761

Abstract

This study investigates the potential of mango leaf extract as a natural preservative to extend the shelf life of milkfish (Chanos chanos) during storage. The extract was prepared using a standardized method and applied to milkfish fillets, followed by an analysis of its effects on microbial growth, sensory attributes, and overall shelf life. The milkfish samples were stored in the refrigerator, and periodic evaluations of sensory quality, including color, texture, odor, and taste, as well as microbiological parameters, were conducted. The findings of this study demonstrate that mango leaf extract effectively inhibits microbial growth and shows potential as a natural preservative for milkfish. The use of 1% mango leaf extract, especially ethanol-based, exhibited the highest microbial inhibition, significantly extending the shelf life and maintaining the quality of milkfish compared to untreated samples. These findings suggest that mango leaf extract can be an effective, natural alternative to chemical preservatives in extending the shelf life of milkfish, offering a promising solution for sustainable seafood preservation.
Co-Authors Adnan Andi Mu'nisa Andi Muflihunna Darminto Darminto, , Dwi Rahayu Dini Endang Sutariningsih Soetarto Fuji Asmiati Ningsih Ismail Ismail Iwan Dini J. Sri Widada M. Junda Megawati Megawati Nani Kurnia Nur Istiqamah Nurhayati B Yahdiel Ronrong