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Polypenol Content Test, Antioxidant Activity Test and Sunblock Protection Test of Pinang Peels Yulianis S.Farm, M.Farm, Apt; Armini Hadriyati; Mukhlis Sanuddin; Nofa Putriani; Indah Sagita Cahyani; Eka Fitriani
Jurnal Ipteks Terapan Vol 14, No 2 (2020): JIT
Publisher : LLDIKTI Wilayah X

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22216/jit.2020.v14i2.5010

Abstract

Areca nut skin contains phenolic compounds which have antioxidant and sunscreen activities. This study aims to determine the levels of polyphenols, antioxidant activity and sunscreen contained in the extract and fraction of areca nut (Areca catechu L.) skin. Testing levels of polyphenols using Folin-Ciocalteau reagents, testing of antioxidants using the DPPH method with a comparison of vitamin C, and on sunscreen testing using the UV-Vis spectrophotometry method by calculating the SPF value. The results of this study indicate that the fraction of n-Butanol has higher polyphenol levels compared to extracts and other fractions with polyphenol levels of 13.88974%. In antioxidant testing it was found that the n-hexane fraction had very strong antioxidant activity with an IC50 value of 40.848 mg / ml. In the sunscreen activity test, it was found that the highest SPF value was at a concentration of 300 ppm, in the thick ethanol extract, n-hexane fraction, ethyl acetate fraction, n-Butanol fraction, and residual water fraction. The highest sunscreen activity of SPF value is the ethyl acetate fraction that is 24.79. From the results of the study it can be concluded that the skin of betel nuts has antioxidant activity, sunscreen, and polyphenol levels.
PENETAPAN KADAR POLIFENOL EKSTRAK DAN FRAKSI KULIT PINANG (Areca catechu L.) DENGAN METODE SPEKTROFOTOMETRI UV-VIS Yulianis Yulianis; Eka Fitriani; Mukhlis Sanuddin
JOURNAL OF HEALTHCARE TECHNOLOGY AND MEDICINE Vol 6, No 1 (2020): APRIL 2020
Publisher : Universitas Ubudiyah Indonesia

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Abstract

Kulit buah pinang (Areca catechu L.) belum dimanfaatkan dengan baik sehingga sebagian besar masih menjadi limbah dan sampah organik. Kulit buah pinang memiliki kandungan senyawa polifenol. Penetapan kadar polifenol perlu dilakukan karena senyawa polifenol dapat berpotensi sebagai antioksidan. Penelitian ini bertujuan untuk mengetahui kadar polifenol pada ekstrak dan fraksi kulit buah pinang. Kulit buah pinang diekstraksi secara maserasi menggunakan pelarut etanol 70%. Penetapan kadar polifenol total ditentukan dengan metode spektrofotometri UV-Vis menggunakan pereaksi Folin-Ciocalteu. Larutan kemudian diukur absorbansi dengan panjang gelombang 756 nm. Hasil penelitian didapat bahwa kadar polifenol total pada ekstrak sebesar 2,395485 %, fraksi n-heksan sebesar 0,100894 %, fraksi etil asetat sebesar 0,994891 %, fraksi n-Butanol sebesar 3,88974 % dan fraksi air sebesar 0,962963 %. Kadar senyawa polifenol terbesar pada sampel ekstrak dan fraksi n-Butanol kulit buah pinang.