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Maryati Kurniadi
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Analisis Zat Warna Sintetik Terlarang Untuk Makanan Yang Beredar Di Pasaran Azizahwati, Azizahwati; Kurniadi, Maryati; Hidayati, Heidi
Majalah Ilmu Kefarmasian Vol. 4, No. 1
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The use of synthetic food colorants is increasing because of its advantages. However, it is found that not all food colorants which are sold in the market are safe to be consumed. The purpose of this research was to identify synthetic food colorant samples which are sold in market and to find out whether they contain safe food colorants or not. Extraction did not need to be done on samples. Analysis was done using color reaction and paper chromatography. Densitometry was done to support the identification result. The result of this research showed that 10 out of 31 samples that were tested contain synthetic food colorants that must not be used for food. The ten samples were seven red colorants (three samples containing Merah K4, the two others containing Rhodamine B, and two samples containing Scarlet GN), one orange colorant (containing Orange G), one yellow colorant (containing Metanil Yellow) and one chocolate colorant (containing Chocolate Brown FB).
Analisis Epigalokatekin Galat Dalam Minuman Teh Hijau Siap Saji Kurniadi, Maryati; Leswara, Nelly D.; Rinayanti, Yola
Majalah Ilmu Kefarmasian Vol. 4, No. 2
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Epigallocatechin gallat (EGCg) is a polyphenol constituent in tea that has antioxidant effect. The purpose of this research was to identify and examine qualitative and quantitative EGCg in green tea preparations which were stored in different condition, that was in a refrigerator and in an open air. The results showed that all samples containing EGCg. The examination of EGCg concentration in the sample was done by thin layer chromatography densitometric method using cellulose plate. N-propanol - acetic acid - water (1:1:5) was the selected eluent. Linear regression equation from calibration curve was y = -1204.071 + 7.495 x, with correlation factor (r) = 0.99911. EGCg concentration from sample that stored in refrigerator sample number one was 0.00293% w/v, sample number two was 0.00298% w/v, and sample number three was 0.00328% w/v. EGCg concentration from sample that stored in open air was lower than LOD.
Analisis Formalin dalam Sampel Ikan dan Udang Segar dari Pasar Muara Angke Suryadi, Herman; Kurniadi, Maryati; Melanie, Yuanki
Majalah Ilmu Kefarmasian Vol. 7, No. 3
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The use of formalin as a food additive has been prohibited by the ministry of health as stipulated in The Indonesia Ministry of Health Regulations No.722/Menkes/Per/IV/88. However, in recent years the Indonesian authorities have found trace amounts of formalin as a preservative in perishable foods such as fish and shrimp. The aim of this research is to identify the use of formalin in fresh fish and shrimp samples sold in Muara Angke Market as the fresh seafood market in Jakarta. The first step of this research was formalin identification in fish and shrimp samples and continued by quantitative analysis to assure the results obtained. Qualitative determination of formalin was carried out by Schryver reagent and the quantitative determination was carried out pectrophotometrically using Nash reagent. Validation of UV-Vis spectrophotometric method for determination of formalin showed that Nash reagent was suitable to determine formalin. The limit of detection, limit of quantitation, and coefficient of variation for formalin were 0,0102 mg/L, 0,0341 mg/L, and 0,09%, respectively. Recovery of formalin in fish samples was 89,79-109,58% and shrimp samples was 82,11-97,76%. Qualitative determination in six fish samples and six shrimp samples showed negative results and the quantitative analysis confirmed that formalin was not found in the fresh fish and shrimp samples from Muara Angke Market.
Optimasi Biotransformasi Total Sterol Limbah Tahu Menggunakan Mycobacterium phlei DSM 43286 Menjadi 1,4-Androstadien-3,17-Dion, Dengan Pengaruh Variasi Konsentrasi Inhibitor A, A’- Dipiridil Kurniadi, Maryati; Tambunan, Usman Sumo Friend; Harmita, Harmita
Majalah Ilmu Kefarmasian Vol. 1, No. 1
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Isolation of total sterol from waste product of soy bean cake has been conducted, followed by biotransformation to 1,4-androstadien-3,17-dione (ADD). The waste product consist of; β-sitosterol, stigmasterol, kaemfesterol, which are isolated by column chromatography technique using silica gel as stationary phase and chloroform as mobile phase. Biotransformation was conducted by using Mycobacterium phlei DSM 43286 in the present of α, α’- dipiridil as an inhibitor with concentration of 0,5; 1; 1,5; 2,0 mM. The main product of biotransformation were ADD and pregnenolon. The optimum yield of ADD 0,48% is achieved by adding 1,5 mM α, α’- dipiridil are two hours after addition of substrate and 72 hours of incubation time.
Analisis Zat Warna Merah Sintetik pada Selai Stroberi yang Dijual di Pasar Tradisional Kota Depok Ayuningtyas, Putri; Sauriasari, Rani; Kurniadi, Maryati
Majalah Ilmu Kefarmasian Vol. 9, No. 2
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Jam is a semi-solid food products made of fruit cooked with sugar that used as aflavoring on bakery including strawberry jam. Strawberry jam is added to food additives such as food dyes. The purpose of this study is to know about ponceau 4R, allura red, rodamin B, and amaran in the sample of strawberry jam in the traditional market at Depok City and to determine the levels of synthetic red dyes that are permitted on the sample strawberry jam. The method applied was dye isolation with wool and followed by analysis using a color reaction, then followed by paper chromatography using mobile phase n-butanol-eth-anol-water (3:4:4) and isobutanol-ethanol-water (3:2:4 ) and also TLC Densitometri using eluent ethanol-n-butanol-water (3:7:1). The result of study that from eight that has been investigated, it was found that six of them was contained ponceau 4R with levels of each sample at 0, 01164; 0, 00469; 0, 00974; 0, 00283; 0, 00482 and 0, 00435% still safe to consume.
Co-Authors Harmita Harmita, Harmita Heidi Hidayati, Heidi Herman Suryadi Loedfiasfiati Alawiyah MARIA BINTANG Melanie, Yuanki Nelly D. Leswara Putri Ayuningtyas Rani Sauriasari, Rani Tambunan, Usman Sumo Friend Yahdiana Harahap Yola Rinayanti, Yola