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All Journal Bioeksperimen: Jurnal Penelitian Biologi Caraka Tani: Journal of Sustainable Agriculture Biosaintifika: Journal of Biology & Biology Education Scripta Biologica AgriHealth: Journal of Agri-food, Nutrition and Public Health
Sugiyono Sugiyono
Department Of Botany, Faculty Of Biology, Universitas Jenderal Soedirman, Purwokerto
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Public Health

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Journal : Scripta Biologica

 1 
UPAYA MEMACU PERTUMBUHAN TUNAS MIKRO KENTANG KULTIVAR GRANOLA DENGAN JENIS DAN KONSENTRASI SITOKININ BERBEDA Adinda RN Pratama; Sugiyono Sugiyono; Lucky Prayoga; Ali Husni
Scripta Biologica Vol 1, No 3 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (719.451 KB) | DOI: 10.20884/1.sb.2014.1.3.553

Abstract

Micro-shoot growth is the first step in the potato microtuber formation. The main factors were known controlling microtuber formation including the media type, the concentration of sucrose, the type and concentration of plant growth regulators, temperature, and photoperiodicity. This research aimed to determine the influence of the t of cytokinin and its concentration on the micro-shoot formation of Granola cultivar of potato (Solanum tuberosum L.), as well as to determine the best cytokinin type and its concentration on micro-shoot formation of Granola cultivar potato (Solanum tuberosum L.). The research was experimental in a split-plot design. The main plot was the type of cytokinin (BAP and Kinetin), and the subplot was cytokinin concentration at five levels of concentrations, i.e., 0, 5, 10, 15 and 20 µM. This study replicated each treatment three times which resulted in obtaining 30 experimental units. The data obtained were then analyzed using an analysis of variance, followed by honest significant difference test at 95 and 99% levels of confidence. The results showed that the formation of micro-shoot of Granola cultivar of potato was controlled by the type and concentration of cytokinin used. The addition of 5‒15 μM of BAP was found to be the best treatments to stimulate micro-shoot formation of Granola cultivar of potato.
The Application of Two Steps Culture in Agarwood, Aquilaria malaccensis, In Vitro Culture Improves Microshoots Induction and Development Vindi Ambara Putri; Sugiyono Sugiyono; Lucky Prayoga; Rendie Prasetyo; Sharon Hilary
Scripta Biologica Vol 9, No 1 (2022)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2022.9.1.1373

Abstract

Agarwood (Aquilaria malaccensis) is an important species with high economic value and has many benefits, which led to an overexploitation in its natural habitat.  An effort to both provides sufficient seedling for agarwood cultivation and conservation is therefore much needed. This study has been carried out with a view to determine the effect of media types and BAP concentrations in two steps cultivation system on agarwood microshoot induction. This was a two stage-experiments i.e., microshoots induction and optimizing shoot development.  The research results showed that the interaction between different media type and BAP concentrations had no significant effect on agarwood microshoot induction.  Subsequent culture on MS media without any BAP addition showed that explant derived from MS medium solidified with 2.5 gr. L-1 Phytagel produced 2.36 ± 0.48 shoots/explant and 3.69 ± 1.16 leaves/explant.  In addition, explant derived from culture on MS medium supplemented with 4 µM BAP produced 2.28 ± 0.61 shoots/explant. This is for the first time the application of two steps culture system for Agarwood (Aquilaria malaccensis) has been deployed and how the habituation phenomenon is handled.
Co-Authors Adinda RN Pratama Ali Husni Andes Khofifah Putri Egi Nuryadin Elly Proklamasiningsih ELLY PROKLAMASININGSIH Elly Proklamasiningsih Elly Proklamasiningsih Erik Harry Murchie Ilham Warfa’ni Khavid Faozi Lucky Prayoga Lucky Prayoga Lucky Prayoga Lucky Prayoga Murchie, Erik Harry Paul A. Davison Prasetyo, Rendie Prita Sari Dewi Prita Sari Dewi Rendie Prasetyo Rendie Prasetyo Sharon Hilary Tamyiz, Maulana Vindi Ambara Putri