Prasetyo, Rendie
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The Improvement of Mini Tuber Production of Granola Potato Cultivar in Aeroponics System Sugiyono, Sugiyono; Prayoga, Lucky; Proklamasiningsih, Elly; Faozi, Khavid; Prasetyo, Rendie
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.27714

Abstract

Potato is an important food crop in both developed and developing countries and it is the fourth most important food crop after wheat, maize, and rice. Potato production in Indonesia has decreased in recent years, mainly due to the use of low quality tuber seed. The objective of this research was to determine the effect of plant materials, types of nutrients, and planting density on mini tuber formation, as well as to determine the best aeroponics conditions for mini tuber formation of Granola cultivar of potato. This research has been carried out experimentally using a Split-split Plot Design. The main plot was planting materials which consisted of plantlet and knol. The subplot was the type of nutrition media which consisted of Farran nutrition, Otazu nutrition, and AB-commercial mix. The sub-sub-plot was planting density which consisted of 50, 75, and 100 plants/m2. The research results showed that knol planted in aeroponics system on AB-commercial mix at a planting density of 75 plants/m2 resulted in the best mini-tuber production. In this study, for the first time three important factors for potato mini-tuber production have thoroughly been investigated in Indonesia. This finding will be used by Kledung Horticultural Seed Garden, Temanggung, for mass production of high-quality mini-tuber seed, which in turn will provide sufficient tuber seed for the farmer in its surrounding areas. By increasing the production of high-quality tuber seed, it is expected that it will contribute to the improvement of potato productivity and increase Indonesian potato production.
Plantlet Formation and Acclimatization of Sugarcane cv. PS 881 with Different Types and Concentration of Auxin Prasetyo, Rendie; Sugiyono, Sugiyono; Proklamasiningsih, Elly; Dewi, Prita Sari
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.23482

Abstract

This research has been carried out with a view to induce rooting and  plantlet formation, followed by acclimatization.  Micro shoots of sugarcane cv. PS 881 were cultured on Murashige and Skoog medium supplemented with different types and concentration of auxins for root induction. This research conducted experimentally using a split-plot design. The main plots were three types of auxins, which consisted of IAA, IBA, and NAA. The subplots were auxin concentrations with four levels, i.e. 0 μM, 5 μM, 10 μM, and 15 μM. Significantly faster root emergence time and higher number of roots observed in the Murashige and Skoog basal medium supplemented with 10 μM NAA. The best root length obtained in the Murashige and Skoog basal medium supplemented with NAA 0 μM. Plantlets derived from NAA 10 μM treatment showed the best performance during acclimation with a 100% survival rate. NAA at a concentration of 10 µM considered to be the best treatment in  plantlet formation and acclimatization of sugarcane cv. PS 881. This study showed that the use of MS medium with 10 uM NAA is able to increase the growth of PS 881 sugarcane plantlets. The results of this study can increase the availability of high quality seedlings and increase national sugar production.
Improving Agarwood (Aquilaria malaccensis Lamk.) Plantlet Formation Using Various Types and Concentrations of Auxins Tamyiz, Maulana; Prayoga, Lucky; Prasetyo, Rendie; Murchie, Erik Harry; Sugiyono, Sugiyono
Caraka Tani: Journal of Sustainable Agriculture Vol 37, No 1 (2022): April
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v37i1.58370

Abstract

Aquilaria malaccensis Lamk. is one of the most widespread agarwood-producing plants that face extinction due to overexploitation. Agarwood propagation using in vitro culture techniques is capable of producing large quantities of plants in a shorter time and free from pests and diseases. Therefore, this study was conducted to analyze the effect of auxins type and concentration on agarwood plantlet formation using a split-plot design. The main plot was the type of auxin which included IAA, IBA and NAA, while the subplot was the concentration used which consisted of 0; 5; 10; 15 and 20 µM. The variable observed was agarwood plantlet formation with parameters measured including the number of shoots and leaves, plant height, and number of roots. The results showed that the formation of agarwood plantlets was controlled by the type, concentration, and interaction between the type and concentration of auxin. Furthermore, explants cultured on Murashige Skoog (MS) medium supplemented with 10 µM IBA produced the highest number of shoots (3.39 shoots explant-1) and leaves (7.25 leaves explants-1), while the addition of 10 uM NAA resulted in the highest number of roots (2.52 roots explant-1). This is the first time a study is conducted to evaluate the effect of type and concentration of auxins on agarwood plantlet formation. The production of high-quality shoots and plantlets increased agarwood germplasm availability to prevent extinction and support sustainable production.
Induksi Pertumbuhan Dan Pembungaan Tagetes sp. Menggunakan Aplikasi Berbagai Konsentrasi Paklobrutazol Dan Giberelin Hidayah, Hexa Apriliana; Rochmatino, Rochmatino; Prasetyo, Rendie
JURNAL VIGOR Vol 7, No 2 (2022): VIGOR: JURNAL ILMU PERTANIAN TROPIKA DAN SUBTROPIKA
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/vigor.v7i2.6514

Abstract

Tagetes sp. merupakan salah satu tanaman hias yang memiliki nilai yang cukup tinggi. Tagetes sp. sering dimanfaatkan secara beragam. Penelitian tahun pertama ini dilakukan dengan tujuan untuk: mempelajari pengaruh interaksi paclobrutazole dan gibberellin terhadap pembungaan Tagetes sp.dan untuk menentukan konsentrasi paclobrutazole dan gibberellin terbaik untuk memacu pembungaan Tagetes sp. Metode penelitian yang digunakan adalah metode eksperimental dengan pola perlakuan RAL faktorial. Sebagai faktor pertama adalah konsentrasi gibberellin terdiri dari 4 taraf yaitu 0 ppm; 50 ppm; 100 ppm; 150 ppm. Sebagai faktor kedua adalah konsentrasi paclobrutazole, yang terdiri dari 3 taraf terdiri dari  0 ppm; 100 ppm; 200 ppm. Setiap kombinasi perlakuan diulang 10 kali sehingga diperoleh 120 unit percobaan. Hasil yang didapat menunjukan bahwa interaksi antara paklobrutazol dan GA3 tidak menunjukan hasil yang berbeda nyata pda semua parameter yang diujikan. Penggunaan GA3 dan paklobrutazol secara mandiri dapat mempengaruhi pertumbuhan Tagetes sp. Perlakuan GA3 100 ppm menunjukan tinggi tanaman dan laju pertumbuhan relatif sebesar 24,40 dan 3,08. Perlakuan paklobrutazol pada konsentrasi 0 ppm dan 200 ppm menunjukan rataan jumlah bunga terbanyak yaitu 10,80, sedangakan untuk rataan jumlah akar terbanyak ditunjukan pada perlakuan paklobrutazol 0 ppm yaitu sebanyak 49,06.  
Kenekaragaman Dan Potensi Bambu Untuk Menyokong Agroekonomi Di Kabupaten Banyumas Hidayah, Hexa Apriliana; Nagri, Dian Ageng; Prasetyo, Rendie
JURNAL VIGOR Vol 7, No 2 (2022): VIGOR: JURNAL ILMU PERTANIAN TROPIKA DAN SUBTROPIKA
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/vigor.v7i2.7037

Abstract

AbstractBambu merupakan tumbuhan yang termasuk dalam subfamilia Bambusoidae dari familia Poaceae. Keanekaragaman Bambu di dunia tergolong tinggi, dengan pemanfaatannya yang beragam. Penelitian ini bertujuan untuk mengetahui keanekaragaman bambu berdasarkan karakter morfologi dan pemanfaatannya di wilayah Kabupaten Banyumas. Penelitian ini dilakukan di beberapa wilayah di Kabupaten Banyumas dengan menggunakan metode survei dengan teknik pengambilan sampel secara purposive sampling. Variabel yang digunakan yaitu morfologi dan bagian bambu yang dimanfaatkan dengan parameter yang diamati yaitu karakter morfologi akar, batang/buluh dan daun bambu serta pemanfaatannya oleh masyarakat. Data yang diperoleh dianalisis secara deskriptif untuk mengetahui keanekaragaman, hubungan kemiripan dianalisa dengan menggunakan Software MEGA 6.0 metode Unweight Pair Group Method Arithmatic Average (UPGMA), pemanfaatan bambu dianalisa secara deskriptif berdasarkan hasil wawancara. Berdasarkan hasil penelitian, terdapat 6 spesies dengan 2 varietas bambu yaitu yaitu Bambusa vulgaris Schrad. Ex Wendl. var. vulgaris (Bambu Ampel), Bambusa vulgaris Schrad. Ex Wendl. var. striata (Bambu kuning), Bambusa multiplex (Lour.) Raeusch. ex J. A. J. H. Schult (Bambu cina/cendani/gendani), Dendrocalamus asper (Schult.) Backer ex Heyne (Bambu Petung), Gigantochloa atter (Hassk.) Kurz (Bambu asak/ater), Gigantochloa apus (J.A. J. H Schult.) Kurz (Bambu Tali) dan Gigantochloa atroviolacea Widjaja (Bambu Wulung/Ireng). Hubungan kemiripan terdekat dimiliki oleh G. apus dan G. atroviolacea dengan nilai disimilaritas 0,11. Hubungan kemiripan terjauh dimiliki oleh B. vulgaris var. vulgaris dengan D. asper dengan nilai disimilaritas 2,19. Masyarakat di Kabupaten Banyumas memanfaatkan bambu sebagai buluhnya bahan bangunan dan kerajinan serta rebung dan daunnya untuk konsumsi. Kata kunci : Agroekonomi, Bambu, Banyumas, Keanekaragaman.