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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences ILMU KELAUTAN: Indonesian Journal of Marine Sciences Bioeksperimen: Jurnal Penelitian Biologi Biosaintifika: Journal of Biology & Biology Education BioEksakta : Jurnal Ilmiah Biologi Unsoed VIGOR: JURNAL ILMU PERTANIAN TROPIKA DAN SUBTROPIKA Journal of Innovation and Sustainable Empowerment
ELLY PROKLAMASININGSIH
Staf Pengajar Fakultas Biologi Unsoed dan Mahasiswa Pascasarjana Biologi UGM
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Veterinary

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PENGARUH ZAT PENGATUR TUMBUH TERHADAP MULTIPLIKASI TUNAS DAN BAHAN PENYANGGA PADA PEMBENTUKAN PLANTLET KANTONG SEMAR ADRIANII (Nepenthes adrianii) DENGAN KULTUR IN VITRO Nuryadin, Egi; Sugiyono, Sugiyono; Proklamasiningsih, Elly
Bioeksperimen: Jurnal Penelitian Biologi Vol 3, No 2: September 2017
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v3i2.5180

Abstract

Nepenthes adrianii (Kantong Semar) merupakan tanaman endemik khas Gunung Slamet, termasuk dalam Convention on International Trade of Endangered Species (CITES) terdapat apendiks I (Tahun 2003) dan II yaitu tanaman ini tergolong hampir punah dan langka. Mengingat besarnya potensi yang dimiliki tanaman ini, maka perlu adanya upaya konservasi untuk mengembangkan dan melestarikannya. Penerapan bioteknologi kultur jaringan atau kultur in vitro merupakan solusi yang tepat untuk melestarikan dan mengembangkan tanaman ini. Kultur in-vitro digunakan untuk multiplikasi tunas dan pembentukan plantlet.Penelitian ini bertujuan untuk: 1) mempelajari pengaruh interaksi BAP dan NAA pada multiplikasi tunas: 2)  menentukan konsentrasi interaksi BAP dan NAA yang paling baik untuk memacu multiplikasi tunas; 3) mempelajari pengaruh bahan penyangga eksplan dan NAA pada pembentukan plantlet kantong semar (Nepenthes adrianii); dan 4) menentukan jenis bahan penyangga eksplan dan konsentrasi zat pengatur tumbuh NAA yang paling baik untuk memacu pembentukan plantlet kantong semar  (Nepenthes adrianii).Metode yang digunakan adalah metode eksperimental, penelitian ini terdiri atas 2 tahap yaitu multiplikasi tunas dan pembentukan plantlet. Tujuan penelitian  pada tahap multiplikasi tunas adalah untuk perbanyakan tunas dan tujuan penelitian pada tahap pembentukan plantlet adalah untuk mendapatkan tanaman kecil yang sejati. Tahap Multiplikasi Tunas menggunakan Rancangan Acak Lengkap (RAL) pola faktorial dengan 2 faktor. Faktor I adalah konsentrasi BAP yang terdiri dari 4 taraf yaitu 0 µM, 5 µM, 10 µM, dan 15 µM, Faktor II  konsentrasi NAA yang terdiri dari 4 taraf yaitu yaitu 0 µM, 0,5 µM, 1 µM, dan 1,5 µM. Tahap Pembentukan Plantlet menggunakan Rancangan Petak Terpisah (Split Plot Design) sebagai petak utama adalah bahan penyangga eskplan (P) yaitu:  agar dan jembatan kertas saring, sedangkan sebagai anak petak adalah konsentrasi NAA yang terdiri dari 4 taraf yaitu 0 µM, 5 µM, 10 µM, dan 15 µM. Parameter yang diukur dalam multiplikasi tunas yaitu waktu muncul tunas, waktu muncul daun, waktu muncul akar, jumlah tunas, jumlah daun dan jumlah akar. Parameter yang diukur dalam pembentukan plantlet yaitu jumlah tunas, jumlah daun, jumlah akar, daun terpanjang, akar terpanjang dan tinggi tanaman. Data yang diperoleh dianalisis menggunakan Analisis Ragam (Anova : Analysis of Variance) dengan  tingkat kepercayaan 95%. Pengujian F menunjukan hasil sangat nyata kemudian dilanjutkan dengan uji Beda Nyata Jujur (BNJ) untuk mengetahui perbedaan antar perlakuan.Hasil penelitian menunjukan bahwa pada tahap multiplikasi tunas terbaik didapat pada perlakuan interaksi BAP 10 mM dan NAA 0,5 mM. Tahap Pembentukan plantlet terbaik di dapat pada perlakuan interaksi bahan penyangga jembatan kertas saring dengan zat pengatur tumbuh NAA 5 mM dan 10 mM.
CADMIUM (CD) ABSORPTION AND PHENOL CONTENT IN POGOSTEMON EXPOSED TO HEAVY METALS Proklamasiningsih, Elly; Budisantoso, Iman; Kamsinah, Kamsinah
Journal of Tropical Life Science Vol 8, No 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.01.02

Abstract

Patchouli (Pogostemon cablin Benth.) is an important plant used by industrial facilities to absorb cadmium (Cd) in polluted land. We performed an experiment using plant medium polluted with both Cd and lead (Pb) with added humic acid. The aims of this study were to 1) determine the effects of humic acid in growth medium contaminated with Cd and Pb on the absorption of Cd and phenol content in patchouli, and 2) determine the Cd tolerance level of the growth media. A completely randomized factorial design was used for the experiment with two factors. The heavy metals were a combination of pure PbNO3 and Cd (PC) with a ratio 1 : 1, and included five concentrations: PC0 (without PbNO3 and without Cd); PC1 (250 ppm PbNO3 + 250 ppm Cd); PC2 (500 ppm PbNO3 + 500 ppm Cd); PC3 (750 ppm PbNO3 + 750 ppm Cd); PC4 (1,000 ppm PbNO3 + 1,000 ppm Cd) and humic acid concentration (0; 6,000; 12,000; and 18,000 ppm). Each treatment was replicated three times. The parameters observed were plant biomass, Cd absorption, and phenol content. The application rate of humic acid to the plant medium containing heavy metals influenced the growth of patchouli, Cd absorption, and phenol content. An application rate of 12,000 ppm of humic acid reduced the toxicity of the heavy metals and increased the dry biomass and phenol content of patchouli.
The Improvement of Mini Tuber Production of Granola Potato Cultivar in Aeroponics System Sugiyono, Sugiyono; Prayoga, Lucky; Proklamasiningsih, Elly; Faozi, Khavid; Prasetyo, Rendie
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.27714

Abstract

Potato is an important food crop in both developed and developing countries and it is the fourth most important food crop after wheat, maize, and rice. Potato production in Indonesia has decreased in recent years, mainly due to the use of low quality tuber seed. The objective of this research was to determine the effect of plant materials, types of nutrients, and planting density on mini tuber formation, as well as to determine the best aeroponics conditions for mini tuber formation of Granola cultivar of potato. This research has been carried out experimentally using a Split-split Plot Design. The main plot was planting materials which consisted of plantlet and knol. The subplot was the type of nutrition media which consisted of Farran nutrition, Otazu nutrition, and AB-commercial mix. The sub-sub-plot was planting density which consisted of 50, 75, and 100 plants/m2. The research results showed that knol planted in aeroponics system on AB-commercial mix at a planting density of 75 plants/m2 resulted in the best mini-tuber production. In this study, for the first time three important factors for potato mini-tuber production have thoroughly been investigated in Indonesia. This finding will be used by Kledung Horticultural Seed Garden, Temanggung, for mass production of high-quality mini-tuber seed, which in turn will provide sufficient tuber seed for the farmer in its surrounding areas. By increasing the production of high-quality tuber seed, it is expected that it will contribute to the improvement of potato productivity and increase Indonesian potato production.
Plantlet Formation and Acclimatization of Sugarcane cv. PS 881 with Different Types and Concentration of Auxin Prasetyo, Rendie; Sugiyono, Sugiyono; Proklamasiningsih, Elly; Dewi, Prita Sari
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.23482

Abstract

This research has been carried out with a view to induce rooting and  plantlet formation, followed by acclimatization.  Micro shoots of sugarcane cv. PS 881 were cultured on Murashige and Skoog medium supplemented with different types and concentration of auxins for root induction. This research conducted experimentally using a split-plot design. The main plots were three types of auxins, which consisted of IAA, IBA, and NAA. The subplots were auxin concentrations with four levels, i.e. 0 μM, 5 μM, 10 μM, and 15 μM. Significantly faster root emergence time and higher number of roots observed in the Murashige and Skoog basal medium supplemented with 10 μM NAA. The best root length obtained in the Murashige and Skoog basal medium supplemented with NAA 0 μM. Plantlets derived from NAA 10 μM treatment showed the best performance during acclimation with a 100% survival rate. NAA at a concentration of 10 µM considered to be the best treatment in  plantlet formation and acclimatization of sugarcane cv. PS 881. This study showed that the use of MS medium with 10 uM NAA is able to increase the growth of PS 881 sugarcane plantlets. The results of this study can increase the availability of high quality seedlings and increase national sugar production.
Morphological and Genetic Diversity of Mangrove Species Ceriops tagal (Perr.) C.B. Rob. Around Java Island Sukmarani, Dhuta; Proklamasiningsih, Elly; Susanto, Agus Hery; Ardli, Erwin Riyanto; Permadi, Jefri; Palimirmo, Flandrianto Sih
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 28, No 4 (2023): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.28.4.334-350

Abstract

Ceriops tagal (Perr.) C.B. Rob can be found in Baluran National Park, Kepulauan Seribu National Park, Karimunjawa National Park, and Segara Anakan Cilacap in Java and its surrounding areas. These locations have different demographics and environmental conditions. Plant species found in different geographical areas have varied genetic structures and morphological traits as a result of their adaptability to their environment. The goal of this study is to evaluate the morphological and genetic differences between the four populations of C. tagal in Java and its surroundings. Internode length, leaf length, leaf width, petiole length, and leaf area were the morphological characters used. The Internal Transcribed Spacer (ITS) region is used as a molecular marker. The UPGMA dendogram was used to analyze the morphological similarity, and multidimensional unfolding analysis was used to determine which character most determined the grouping. Mega X software was used to analyze genetic variation using the neighbor-joining approach, while Arlequin 3.5 software was used to estimate genetic variation within and among populations. The results of this study showed that the population of Baluran National Park has features or is distinct from the other three populations based on morphological and genetic characters; this could be because of variations in geographic conditions, and that population diversification was aided by a combination of genetic and physical divergence, restricted gene flow, and local adaptation.
Respon Akar Kiara Payung (Filicium decipiens (Wight & Arn.) Thwaites) Pada Kondisi Cekaman Natrium Hipoklorit Widhodho, Joko; Proklamasiningsih, Elly; Widodo, Pudji
JURNAL VIGOR Vol 7, No 2 (2022): VIGOR: JURNAL ILMU PERTANIAN TROPIKA DAN SUBTROPIKA
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/vigor.v7i2.6706

Abstract

Sodium hypochlorite (NaClO) was a chemical compound commonly used as a whitening and disinfectant agent. Kiara payung (Filicium decipiens), or the ferntree, was chosen as the object of research because easily obtained, has rapid growth, and thought to respond to alien substances' stress. The main purpose of this study was to find out the response of the ferntree plant, which is given stress from sodium hypochlorite. The research method used in this study was experimentation. The independent variable in this study was Sodium Hypochlorite Concentration (X), and the dependent variable was Sodium Hypochlorite Uptake Value (Y). Phytochemical analysis was performed using the argentometry and Atomic Absorption Spectrophotometry (AAS) techniques. Correlation analysis were performed using IBM SPSS 26. This study showed that the concentration of sodium hypochlorite has a positive effect on the uptake value of sodium hypochlorite. Limitations and suggestions to this study were to check the absorption based on soil level and examine other parts of the plant in the future study. In addition, another factors to the independent variables, such as genetics and variations in sodium hypochlorite concentration should be further explored, so that correlation analysis stands more optimal. Keywords: Ferntree, Root, Sodium Hypochlorite, Stress
Pengaruh Pemberian Asam Humat pada Media Tanam terhadap Pertumbuhan dan Kandungan Polifenol Daun Binahong (Anredera cordifolia) Riyandi, FNU; Proklamasiningsih, Elly; Rochmatino, Rochmatino
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.2.1967

Abstract

Tanaman Binahong (Anredera cordifolia) merupakan salah satu tanaman obat yang bermanfaat sebagai antioksidan. Kandungan senyawa aktif utama pada daun Binahong yaitu flavonoid yang merupakan salah satu senyawa golongan polifenol. Jenis flavonoid yang terkandung pada daun Binahong adalah flavonol. Penggunaan asam humat dapat meningkatkan kualitas media tanam sehingga pertumbuhan tanaman dapat meningkat. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh asam humat terhadap pertumbuhan dan kandungan polifenol daun Binahong dan menentukan konsentrasi asam humat yang efektif untuk meningkatkan pertumbuhan dan kandungan polifenol daun Binahong. Penelitian ini dilakukan di Greenhouse dan Laboratorium Fisiologi Tumbuhan Fakultas Biologi Universitas Jenderal Soedirman. Metode yang digunakan pada penelitian ini adalah metode eksperimental dengan menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari empat perlakuan asam humat pada media pasir dengan konsentrasi 0 g. kg-1; 4 g. kg-1; 8 g. kg-1; 12 g. kg-1 dan diulang sebanyak tiga kali. Parameter yang diamati pada penelitian ini adalah pertumbuhan tanaman meliputi jumlah daun, berat basah dan berat kering tanaman, serta kandungan polifenol pada daun Binahong. Data yang diperoleh dianalisis ragam atau Analisis of variance (ANOVA) dengan taraf uji 1%, kemudian dilanjutkan dengan uji Beda Nyata Terkecil (BNT) dengan taraf uji 5%. Hasil penelitian menunjukkan bahwa pemberian asam humat pada media tanam mampu meningkatkan pertumbuhan dan kandungan polifenol daun Binahong dengan konsentrasi 12 g.kg-1.
INOKULASI RIZOBAKTERIA PADA TANAMAN CABAI (Capsicum annum L.) DI TANAH SALIN anti, Monica Widi; Oedjijono, Oedjijono; Proklamasiningsih, Elly
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.2.2140

Abstract

A red chili plant (Capsicum spp.) is one of the horticultural commodities in Indonesia which has good economic value, so it has priority to be developed. Agriculture practices in Java island have partly switched to coastal areas which have characteristics of saline soils. Saline soils are alkaline and nutrient-poor, especially essential nutrients such as P and N. One way to restore fertility in saline soils used beneficial and fertilizing bacteria such as PGPR. Plant growth-promoting rhizobacteria (PGPR) are free-living, soil-borne bacteria, which are capable of enhancing the growth of the plant either directly or indirectly. The objectives of this research were to determain the effect of PGPR isolates to the growth of red chili plants in saline sandy soil and to determine the best PGPR isolate capable to improve the growth of red chili plant in saline sandy soil. The result showed that the inoculation of rhizobacteria significantly affected to plant height and root length of the red chili plant (Capsicum annum). The combination of 3 PGPR isolates (Azospirillum PSA 10, Azotobacter PSA 8, and Marinococcus PSA 1) was the best result in increasing the height and root length of the chili plant (Capsicum annum).
Dampak Pemanasan Global terhadap Fenologi Tanaman Kecipir (Psopocarpus tetragonolobus) dan Hubungannya dengan Serangga Pollinator Rahayuningtyas, Yuni Rokhdita; Eming Sudiana, Eming; Proklamasiningsih, Elly
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 3 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3171

Abstract

Global warming or often referred to as global warming is a form of imbalance of ecosystems on earth due to the process of increasing the average temperature of the atmosphere, sea, and land on earth. The impact of global warming can make agricultural plants flower faster while pollinating insects are not ready so that the reproductive cycle is disrupted. Studying temperature changes as a result of global warming on an organism can be represented by an altitude gradient. The study aims to determine the effect of temperature changes on the phenology of winged bean plants (Psophocarpus tetragonolobus) which is described by the gradient of altitude and to determine the diversity of pollinator insects. The independent variable in this study is the difference in the gradient in elevation of the place, while the dependent variable is the phenology of the development of winged bean flowers and pollinator insects. The results of the study of abiotic factors showed that air temperature and sunlight intensity decreased in line with the increase in altitude from the surface of the seawater, while the humidity increased. The phenological analysis of winged bean plants showed the influence of altitude on plant height, number of leaves and branches, time of the first appearance, number of flowers and flower size as well as the diversity of pollinator insects.
Respon Penambahan Asam Humat terhadap beberapa Kandungan Senyawa Aktif Pegagan (Centella asiatica) hardiyati, triani; Proklamasiningsih, Elly; Budisantoso, Iman
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 3 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3536

Abstract

The aim of this research is to know the respon of humic acid and some growth media on the contain of active biological substance in Centella asiatica (L.) Urb. Hopefully, through the application of humic acid in the combination of media, the growth and plant metabolism will improve to produce active biological substance as primar metabolism. The growth media used were : soil & sand (1 : 1) ; soil & husk (1 : 1 ) ; and soil & zeolite (1 : 2). The humic acid treatment were 4 concentration, 0 g/kg media, 4 g/kg media, 8 g/kg media and 12 g/kg media. The research method was Completely Randomized Design, with factorial as treatment design. The variable observed were alkaloid content, flavonoid content, the presence of terpenoid, and biomass weight. The research result showed that the high weight of biomass and alkaloid contain got in the addition of 8 g humic acid in media soil and sand. The high flavonoid contain got in addition of 4 g/kg media in media soil and zeolite. There was brawn ring in the extract analysis dissolved in chloroform, acetic acid, and strong sulfate acid.
Co-Authors AGUS HERY SUSANTO anti, Monica Widi Ardli, Erwin Riyanto Dewi, Utami Sari Dhuta Sukmarani, Dhuta Eming SUDIANA, Eming Eming Sudiana, Eming Fadhllurohman, Muhammad Faiq Flandrianto Sih Palimirmo, Flandrianto Sih Imam Widhiono Iman Budisantoso Jannah, Herna Maghfira Raudhatul Jefri Permadi Juwarno, Juwarno Kamsinah Kamsinah Karismaningrum, Unun Kartika Sari, Lilik Khavid Faozi Khoerunnisa ., Khoerunnisa Lucky Prayoga Nurfithri Hashifah, Fathimah Nurtjahjo Dwi Sasongko Nuryadin, Egi Oedjijono Oedjijono, Oedjijono Prasetyo, Rendie Prita Sari Dewi Pudji Widodo Rahayuningtyas, Yuni Rokhdita Retna Utarini Suci Rahayu, Diana Riyandi, FNU Rochmatino Rochmatino, Rochmatino Shinta, Deyana Rose Slamet Santoso Sugiyono Sugiyono Sugiyono Sugiyono Triani Hardiyati Widhodho, Joko