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All Journal JBIO: jurnal biosains (the journal of biosciences) Primary: Jurnal Pendidikan Guru Sekolah Dasar
Nabila Ayu
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Medicine & Pharmacology Other

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DETECTION OF PIG DNA FRAGMENTS IN HALAL UNLABLED LIPSTICK SAMPLES USING CONVENTIONAL PCR Misbakhul Munir; Siti Malihatus Sa'adah; Siti Latifa; Nabila Ayu; Oki Rahmatirta W; Najwa Maulidina P; Ameliora C E; Eko Prasetya; Yuanita Rachmawati
JBIO: jurnal biosains (the journal of biosciences) Vol 7, No 1 (2021): Jurnal Biosains
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v7i1.23707

Abstract

From a Muslim perspective, it is very important to know the content, raw materials, and processing of the raw materials used in the cosmetic products used. One type of cosmetics that is most often used is lipstick. However, many lipsticks circulating in Indonesia are not equipped with a halal logo. One of the ingredients for lipstick is pork derivatives. These pig derivatives can be detected using PCR. Based on this background, this study aims to test the presence of pig DNA in lipstick samples that have not been certified halal on the market using 4 combinations of pig DNA fragments coding primers by using the conventional PCR method. Five commercial lipstick samples were selected by purposive sampling. DNA isolation was carried out according to the Wizard Promega Universal Kit. The PCR process was carried out with temperature optimization as follows: Predenaturation 98oC: 2 minutes, denaturation of 95oC: 30 seconds, Annealing 61oC: 30 seconds, Extension 72oC: 40 seconds, and Postextension 72oC: 3 minutes. The results showed that of the 5 samples tested by PCR using 5 kinds of primer combination, none of the samples were suspected to contain pork DNA. DNA isolation is the most difficult step in the lipstick sample detection process. Even though the detection result is negative, it is necessary to carry out further tests which become the Gold Standard of DNA-based testing using Real Time PCR.
THE CORRELATION BETWEEN LEARNING TIME MANAGEMENT AND STUDENTS’ PERCEPTION OF TEACHER’S PROFESSIONAL COMPETENCY WITH HIGH CLASS LEARNING OUTCOMES Nabila Ayu; Nelly Astuti
Primary: Jurnal Pendidikan Guru Sekolah Dasar Vol. 9 No. 3 (2020): June
Publisher : Laboratorium Program Studi Pendidikan Guru Sekolah Dasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33578/jpfkip.v9i1.7886

Abstract

The problem in this research is the learning outcomes of several students in SDN 3 Adipuro that do not meet the completeness standard. The purpose of this study is to identify the correlation between learning time management and the students’ perception of teacher’s professional competence with high class learning outcomes at SDN 3 Adipuro. This type of research was ex post facto corelation. The population was 115 students which 56 students were chosen as the sample. Data collection techniques used were observation, interviews, questionnaires, and documentation studies. Data collection instruments in the form of a questionnaire with a Likert scale has been claimed validity and reliability. Analysis of the data used was Poduct Moment Correlation and Multiple Correlation. The result showed that there was a positive and significant relationship between learning time management and the students’ perception of teacher’s professional competence with high class learning outcomes with a correlation coefficient of 0.534 according to the "strong enough" level.
Co-Authors Ameliora C E Eko Prasetya Misbakhul Munir Najwa Maulidina P Nelly Astuti Oki Rahmatirta W Siti Latifa Siti Malihatus Sa'adah Yuanita Rachmawati