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Antibacterial activity test on ethanol extract fraction of Kirinyuh (Chromolaena odorata L.) leaves for multi-drug resistant organisms bacteria Endang Sulistyarini Gultom; Tri Hartanti; Hasnaul Maritsa; Eko Prasetya
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 1 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i1.17067

The resistance of pathogenic bacteria to antibiotics is increasing due to antibiotics with incorrect doses, wrong diagnostics, and the wrong target. Bacteria that have been resistant to several antibiotics are called multi-drug resistant organisms (MDRO) bacteria. Bacterial resistance to some antibiotics requires alternative herbal treatments, one of which is the Chromolaena odorata L. Research must therefore be conducted on the antibacterial activity of the ethanol extract fraction of C. odorata L. leaves for MDRO bacteria, such as Staphylococcus lugdunensis methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa extended-spectrum beta-lactamase (ESBL), and Klebsiella pneumoniae ESBL. This study aims to determine the antibacterial activity of the ethanol extract of Kirinyuh leaves (C. odorata L.) and the antibacterial activity of the fractionation against MDRO bacteria. Test results of kirinyuh leaf ethanol extract for S. lugdunensis MRSA, P. aeruginosa ESBL, and K. pneumoniae ESBL bacteria each resulted in an inhibition zone with an average diameter of 11.6 mm (strong), 11.5 mm (strong), and 11.13 mm (strong), respectively. Testing the antibacterial activity of the ethanol fraction against MDRO bacteria can show antibacterial activity against all tested bacteria, namely Fraction 5. The results of the antibacterial activity of fraction 5 against K. pneumoniae ESBL, P. aeruginosa ESBL, and S. lugdunensis MRSA bacteria with the formation of inhibition zones formed of 10.2 mm (strong), 8.8 mm (moderate), and 7.9 mm (moderate), respectively. The results of thin-layer chromatography showed that the secondary metabolites contained in the fifth fraction were terpenoids, steroids, and flavonoids.

ISSR based analysis of genetic variability of plantlets culture of pineapple (Ananas comosus L.) from Sipahutar, North Sumatera, Indonesia Fauziyah Harahap; Annisa Afiva; Miftahul Jannah; Eko Prasetya
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 1 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i1.17068

Sipahutar pineapple is very popular in North Sumatra, because of the distinctive sweet taste and normal water content. Furthermore, it is large in size and has a yellow fruit skin color with greenish tips. However, the problem with Sipahutar pineapple production is the limited amount of good quality seeds. The utilization of in vitro culture techniques on pineapples allows the emergence of somaclonal variations, especially in plantlets that have undergone the subculture stage. This somaclonal variation is one of the problems in commercial seedling production, therefore, it is necessary to conduct an initial examination using the inter simple sequence repeat (ISSR) molecular marker. This study aimed to determine the genetic and primary stability of ISSR, which can be used in pineapple plants native to Sipahutar. The methods used include sample preparation, DNA isolation, primer optimization, ISSR primer amplification by PCR method, and electrophoresis. Furthermore, a total of 15 samples were amplified with six ISSR primers, and the data were analyzed by cluster method using the NTSYS-PC software. The final result was visualized in a dendrogram and analysis of diversity was conducted using GenAlex. The results showed that the level of genetic variability of the Sipahutar pineapple, which has undergone in vitro culture using six ISSR molecular markers was 76-97%. Meanwhile, the genetic variability level of the native to Sipahutar pineapple can be influenced by the long culture period and the use of N6-benzyladenine. The primers can be used to observe the genetic variability, except for ISSR 25 with a PIC value of 0.000.

Analysis of genetic diversity of bacteria Xanthomonas oryzae pv. oryzae causes leaf blight in North Sumatra Zulheri Noer; Maimunah Maimunah; Erwin Pane; Eko Prasetya
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 2 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i2.24092

Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial leaf blight on rice plants, which is responsible for crop failure, resulting in economic losses. Control of Xoo bacteria can be accomplished through the use of Xoo-resistant rice varieties. Due to the shifting genetic structure and environmental conditions of Xoo bacteria, it is difficult to identify them according to their pathotype. This study aims to determine the genetic diversity of Xoo bacteria using the polymerase chain reaction (PCR) method and the IS1112-based RAPD marker, which is known to contain repetitive sequences. DNA was isolated from 52 isolates collected from 15 districts and cities in North Sumatra province and then used to amplify the IS1112 sequence. Dendrogram analysis revealed that cluster analysis of PCR findings classified isolates into 15 groups with a 90% similarity value. Genetic variation among Xoo isolates from North Sumatra ranged between 51% and 100%. North Sumatra Xoo isolates exhibited a high degree of genetic diversity. This findings can be used as a resource for future management of the Xoo bacteria by expediting disease-resistant rice breeding projects in various rice producing countries.

DETECTION OF PIG DNA FRAGMENTS IN HALAL UNLABLED LIPSTICK SAMPLES USING CONVENTIONAL PCR Misbakhul Munir; Siti Malihatus Sa'adah; Siti Latifa; Nabila Ayu; Oki Rahmatirta W; Najwa Maulidina P; Ameliora C E; Eko Prasetya; Yuanita Rachmawati
JBIO: jurnal biosains (the journal of biosciences) Vol 7, No 1 (2021): Jurnal Biosains
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v7i1.23707

From a Muslim perspective, it is very important to know the content, raw materials, and processing of the raw materials used in the cosmetic products used. One type of cosmetics that is most often used is lipstick. However, many lipsticks circulating in Indonesia are not equipped with a halal logo. One of the ingredients for lipstick is pork derivatives. These pig derivatives can be detected using PCR. Based on this background, this study aims to test the presence of pig DNA in lipstick samples that have not been certified halal on the market using 4 combinations of pig DNA fragments coding primers by using the conventional PCR method. Five commercial lipstick samples were selected by purposive sampling. DNA isolation was carried out according to the Wizard Promega Universal Kit. The PCR process was carried out with temperature optimization as follows: Predenaturation 98oC: 2 minutes, denaturation of 95oC: 30 seconds, Annealing 61oC: 30 seconds, Extension 72oC: 40 seconds, and Postextension 72oC: 3 minutes. The results showed that of the 5 samples tested by PCR using 5 kinds of primer combination, none of the samples were suspected to contain pork DNA. DNA isolation is the most difficult step in the lipstick sample detection process. Even though the detection result is negative, it is necessary to carry out further tests which become the Gold Standard of DNA-based testing using Real Time PCR.

DNA BARCODING EDELWEISS (Anaphalis longifolia) ASAL SUMATERA UTARA MENGGUNAKAN SEKUEN GEN maturase K Eko Prasetya; Hary Prakasa; Miftahul Jannah; Yuanita Rachmawati
JBIO: jurnal biosains (the journal of biosciences) Vol 6, No 3 (2020): Jurnal Biosains
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v6i3.22403

Anaphalis longifolia merupakan anggota dari family Asteraceae yang tersebar di dataran tinggi Eropa, Amerika, hingga Asia. Penelitian tentang tanaman ini masih terbatas pada studi habitat, sedangkan penelitian terkait identifikasi molekuler masih belum dilakukan. Penelitian ini bertujuan untuk menganalisis DNA barcode dari A. longifolia menggunakan sekuen matK gene. Sampel yang diperoleh dari Sumatera Utara kemudian di Isolasi DNA, di amplifikasi menggunakan primer spesifik, lalu disequencing. Hasil sequencing dianalisis menggunakan program Molecular Evolution Genetics Analysis (MEGA) Version X. Hasil penelitian menunjukkan bahwa sekuen matK gen berhasil diamplifikasi pada panjang 800-850 kb. Hasil analisis pohon filogenetik menunjukkan bahwa sekuen matK gene dapat mengelompokkan A. longifolia. Pada sekuen matK gene A. longifolia, AT content lebih tinggi dibandingkan dengan GC conten. Jarak genetik yang diperoleh berkisar 0-0.0014. Hasil analisis alignment sekuen matK gene menunjukkan terdapat 1521 karakter yang dapat diamati, 1403 karakter conserved site, 118 karakter variable site, 9 karakter parsimony informative site, dan 7 karakter single nucleotide polymorphism (SNP) site. Sekuen matK gene dapat digunakan sebagai DNA barcoding untuk mengidentifikasi A. longifolia. Hasil penelitian ini diharapkan dapat memberikan informasi penting dalam konservasi A. longifolia.

DNA BARCODING of Zingiber loerzingii Valeton USING Ribulose-1,5-biphosphate Carboxylase-Oxygenase Large subunit Gene (rbcL) GENE LOCUS Ladiez Rahmayani Sagala; Lazuardi Lazuardi; Fauziyah Harahap; Kartika Manalu; Zahratul Idami; Eko Prasetya
JBIO: jurnal biosains (the journal of biosciences) Vol 7, No 3 (2021): Jurnal Biosains
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v7i3.28921

DNA barcode is one of the molecular techniques used to identify and classify living things. Z. loerzingii is currently reported as a rare and endemic plants that is only found in North Sumatra. Scientific studies for Z. Loerzingii is measly done so that the information about this plant is limited while a clear identity on a plant is essential to discover the potential. This study aims to determine the molecular characteristics of Z. loerzingii by using DNA barcodes and assessing the phylogenetic relationship based on the rbcL gene locus. DNA was isolated with a commercial kit. The rbcL gene locus in the chloroplast genome of Z. loerzingii amplified using the Polymerase Chain Reaction technique to produce amplicon with the length approximately 600 bp. Consensus sequence merging generate a sequence with 576 bp length. The phylogenetic tree reconstruction was carried out using the Neighbor-Joining method and the Kimura-2-Parameter calculation model showed Z. loerzingii included in the monophyletic group with Zingiber mioga and Zingiber officinale as the sister taxa. The results for the molecular diversity analysis of Z. Loerzingii point out that in all samples of Z. loerzingii which collected from Cagar Alam Sibolangit have no molecular or genetic diversity. Therefore, it can be concluded that DNA barcoding with the rbcL gene locus can be used as a method to identify Z. loerzingii molecularly and efficient in determining their phylogenetic with other species.

Usnea in West Java: a potential source of bioactive secondary metabolites Miftahul Jannah; Qurrota A`yun; Nida Afifah; Eko Prasetya; Muhammad Rifqi Hariri
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 28 No 1 (2022): December 2022
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/bphjbr.28.1.20224

Lichen is a symbiotic organism consisting of algae (photobionts) and fungi (mycobiont). In Indonesia, Usnea is the only genus of lichen that has been widely recognized and used by the local community for traditional medicine. It is locally known as kayu angin, rusuk angin, or janggot Kai. Furthermore, it produces lichen acid and other compounds as secondary metabolites that need investigation. Therefore, this study aimed to identify species and determine the secondary metabolites of Usnea in West Java. There were 13 species of Usnea identified, with U. rubrotincta, U. mutabilis, and U. barbata being new to West Java. The extraction of U. esperantiana has the highest number of lichen acids, with 20 different types identified. This study showed that Usnea contains lichen acid, flavonoid, and saponin compounds. The secondary metabolites are a potential source of natural products for bioprospecting.

Land Suitability Analysis for Marine Tourism on Kalimantung Island, Central Tapanuli Regency, North Sumatera Province, Indonesia Muhammad Ridha Syafii Damanik; Eko Prasetya; Teguh Febri Sudarma
JURNAL GEOGRAFI Vol 16, No 1 (2024): JURNAL GEOGRAFI
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jg.v16i1.50475

This study aims to spatially analyze the marine ecotourism potential of Kalimantung Island based on the Tourist Suitability Index (TSI). The method used in this research is descriptive qualitative, where the analysis is done with a spatial approach. Data collection techniques used were measurement, observation, and interviews. The data analysis carried out in this study uses scoring techniques. Land suitability analysis is categorized into 4 (four) designations, namely (1) Beach Tourism for Recreation Category, (2) Marine Tourism for diving, (3) Marine Tourism for snorkeling, and (4) Marine Tourism for swimming and canoeing category. The results of this study indicate that the condition of coral reefs on Kalimantung Na Godang Island and Kalimantung Na Menek Island is included in the good category, with coral cover of 55.55% and 52.13 respectively. The Tourism Suitability Index (TCI) for the Recreation Category on Kalimantung Na Godang Island is categorized as suitable with a TSI of 69%. The Recreation TSI of Kalimantung Na Menek Island is categorized as Very Suitable with a Recreation TSI of 95%. The Tourism Suitability Index (TSI) for the Diving Category on Kalimantung Na Godang Island and Kalimantung Na Menek Island is included in the Very Suitable category with a TSI of 83%. The Tourism Suitability Index (TSI) of the Snorkeling Category on Kalimantung Na Godang Island is included in the suitable category with a TSI of 68%. The Recreation TSI of Kalimantung Na Menek Island is included in the Very Suitable category with a Recreation TSI of 75%. The Tourism Suitability Index (TSI) Swimming- Canoeing Category on Kalimantung Na Godang Island and Kalimantung Na Menek Island are included in the Very Suitable category with TSI of 76% and 87% respectively.This study aims to spatially analyze the marine ecotourism potential of Kalimantung Island based on the Tourist Suitability Index (TSI). The method used in this research is descriptive qualitative, where the analysis is done with a spatial approach. Data collection techniques used were measurement, observation, and interviews. The data analysis carried out in this study uses scoring techniques. Land suitability analysis is categorized into 4 (four) designations, namely (1) Beach Tourism for Recreation Category, (2) Marine Tourism for diving, (3) Marine Tourism for snorkeling, and (4) Marine Tourism for swimming and canoeing category. The results of this study indicate that the condition of coral reefs on Kalimantung Na Godang Island and Kalimantung Na Menek Island is included in the good category, with coral cover of 55.55% and 52.13 respectively. The Tourism Suitability Index (TCI) for the Recreation Category on Kalimantung Na Godang Island is categorized as suitable with a TSI of 69%. The Recreation TSI of Kalimantung Na Menek Island is categorized as Very Suitable with a Recreation TSI of 95%. The Tourism Suitability Index (TSI) for the Diving Category on Kalimantung Na Godang Island and Kalimantung Na Menek Island is included in the Very Suitable category with a TSI of 83%. The Tourism Suitability Index (TSI) of the Snorkeling Category on Kalimantung Na Godang Island is included in the suitable category with a TSI of 68%. The Recreation TSI of Kalimantung Na Menek Island is included in the Very Suitable category with a Recreation TSI of 75%. The Tourism Suitability Index (TSI) Swimming- Canoeing Category on Kalimantung Na Godang Island and Kalimantung Na Menek Island are included in the Very Suitable category with TSI of 76% and 87% respectively. Keywords: Spatial Analysis; Land Suitability; Region's Carrying Capacity; Marine Ecotourism

Land Suitability Analysis for Marine Tourism on Kalimantung Island, Central Tapanuli Regency, North Sumatera Province, Indonesia Nama Penulis; Muhammad Ridha Syafii Damanik; Eko Prasetya; Teguh Febri Sudarma
JURNAL GEOGRAFI Vol. 16 No. 1 (2024): JURNAL GEOGRAFI
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jg.v16i1.50475

This study aims to spatially analyze the marine ecotourism potential of Kalimantung Island based on the Tourist Suitability Index (TSI). The method used in this research is descriptive qualitative, where the analysis is done with a spatial approach. Data collection techniques used were measurement, observation, and interviews. The data analysis carried out in this study uses scoring techniques. Land suitability analysis is categorized into 4 (four) designations, namely (1) Beach Tourism for Recreation Category, (2) Marine Tourism for diving, (3) Marine Tourism for snorkeling, and (4) Marine Tourism for swimming and canoeing category. The results of this study indicate that the condition of coral reefs on Kalimantung Na Godang Island and Kalimantung Na Menek Island is included in the good category, with coral cover of 55.55% and 52.13 respectively. The Tourism Suitability Index (TCI) for the Recreation Category on Kalimantung Na Godang Island is categorized as suitable with a TSI of 69%. The Recreation TSI of Kalimantung Na Menek Island is categorized as Very Suitable with a Recreation TSI of 95%. The Tourism Suitability Index (TSI) for the Diving Category on Kalimantung Na Godang Island and Kalimantung Na Menek Island is included in the Very Suitable category with a TSI of 83%. The Tourism Suitability Index (TSI) of the Snorkeling Category on Kalimantung Na Godang Island is included in the suitable category with a TSI of 68%. The Recreation TSI of Kalimantung Na Menek Island is included in the Very Suitable category with a Recreation TSI of 75%. The Tourism Suitability Index (TSI) Swimming- Canoeing Category on Kalimantung Na Godang Island and Kalimantung Na Menek Island are included in the Very Suitable category with TSI of 76% and 87% respectively.This study aims to spatially analyze the marine ecotourism potential of Kalimantung Island based on the Tourist Suitability Index (TSI). The method used in this research is descriptive qualitative, where the analysis is done with a spatial approach. Data collection techniques used were measurement, observation, and interviews. The data analysis carried out in this study uses scoring techniques. Land suitability analysis is categorized into 4 (four) designations, namely (1) Beach Tourism for Recreation Category, (2) Marine Tourism for diving, (3) Marine Tourism for snorkeling, and (4) Marine Tourism for swimming and canoeing category. The results of this study indicate that the condition of coral reefs on Kalimantung Na Godang Island and Kalimantung Na Menek Island is included in the good category, with coral cover of 55.55% and 52.13 respectively. The Tourism Suitability Index (TCI) for the Recreation Category on Kalimantung Na Godang Island is categorized as suitable with a TSI of 69%. The Recreation TSI of Kalimantung Na Menek Island is categorized as Very Suitable with a Recreation TSI of 95%. The Tourism Suitability Index (TSI) for the Diving Category on Kalimantung Na Godang Island and Kalimantung Na Menek Island is included in the Very Suitable category with a TSI of 83%. The Tourism Suitability Index (TSI) of the Snorkeling Category on Kalimantung Na Godang Island is included in the suitable category with a TSI of 68%. The Recreation TSI of Kalimantung Na Menek Island is included in the Very Suitable category with a Recreation TSI of 75%. The Tourism Suitability Index (TSI) Swimming- Canoeing Category on Kalimantung Na Godang Island and Kalimantung Na Menek Island are included in the Very Suitable category with TSI of 76% and 87% respectively. Keywords: Spatial Analysis; Land Suitability; Region's Carrying Capacity; Marine Ecotourism

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