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All Journal Jurnal Pendidikan IPA Indonesia Journal of the Indonesian Tropical Animal Agriculture Tropical Animal Science Journal
A. Kurniawati
Prodi Pendidikan IPA, FMIPA Universitas Negeri Semarang
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Education Energy

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Journal : Tropical Animal Science Journal

 1 
Morphological and Molecular Diversity of Five Superior Napier Grass Cultivars in Indonesia Nasution, M. D. M.; Umami, N.; Kurniawati, A.; Rahman, M. M.
Tropical Animal Science Journal Vol. 48 No. 1 (2025): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2025.48.1.8

Abstract

This study aimed to evaluate the morphological and genetic diversity among five cultivars of Napier grass (Pennisetum purpureum) grown in Indonesia: ‘Gama Umami’, ‘Pakchong’, ‘Odot’, ‘Purple’, and ‘Local’. A total of 20 plants per cultivar were planted in a completely randomized design with morphological parameters, nutrient content, biomass production, and molecular analysis assessed at a cutting age of 90 days. The morphological analysis revealed significant differences (p<0.05) in plant height, leaf length, and the number of tillers across the cultivars. Qualitative analysis revealed differences in leaf color and growth habits. ‘Gama Umami’ cultivar showed the highest biomass yield, with significantly (p<0.05) higher crude protein and dry matter content than the other cultivars. Random amplified polymorphic DNA (RAPD) analysis using nine primers on the five Napier grass cultivars demonstrated diverse band patterns, resulting in a percentage of polymorphic bands (PBP) ranging from 60% to 100%. The dendrogram derived from the RAPD data clustered the cultivars into two main groups, with ‘Gama Umami’ and ‘Local’ showing a high similarity coefficient of 0.73, while ‘Purple’ and ‘Pakchong’ formed a distinct sub-cluster with a similarity coefficient of 0.66, and ‘Odot’ exhibited a similarity coefficient of 0.58 with the ‘Purple’ and ‘Pakchong’ sub-cluster. This study revealed significant genetic and morphological diversity among five Napier grass cultivars, with 'Gama Umami' demonstrating superior morphological traits, nutrient content, and biomass production. These findings highlight the potential of integrating molecular and morphological analyses to support breeding programs for improving forage quality and productivity.
Preservation of High-Moisture Sorghum Silage Using Combination of Biological and Chemical Additives in the Tropical Region Ardiansyah, M.; Fitriani, D.; Noviandi, C. T.; Kurniawati, A.; Paradhipta, D. H. V.
Tropical Animal Science Journal Vol. 48 No. 3 (2025): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2025.48.3.257

Abstract

This study aimed to investigate the effect of the biological and chemical additives on fermentation characteristics, aerobic stability, and ruminal digestibility of high-moisture sorghum silage. A mixture of Lactiplantibacillus plantarum FNCC 0020 (LP) and Limosilactobacillus fermentum BN21 (LF) was used as a biological additive and potassium sorbate as a chemical additive. At the milk ripening stage (26.8% of DM), sorghum was harvested, chopped to 3-5 cm length, and ensiled into 20 L silo (5 kg) for 100 days. Subsequently, various additives were added, including a control group without additives (CON), LF + LP with a ratio of 1:1 at 1 x 105 cfu/g fresh weight (INO), potassium sorbate at 1 g/kg fresh weight (PS), and INO + PS (MIX). Each treatment used 5 silos as replication. The results showed that INO silage had the lowest (p<0.05) pH with the highest (p<0.05) contents of lactate and acetate, as well as lactic acid bacteria (LAB) count. PS silage produced the minimum contents of lactate and acetate but had lower yeast compared to CON silage. MIX silage had lower (p<0.05) lactate and acetate contents than CON silage, with a similar effect on yeast inhibition to PS silage. Furthermore, PS and MIX silages had higher (p<0.05) aerobic stability and in vitro digestibility of dry matter and organic matter than CON and INO. These results showed that combining biological and chemical additives was more effective in improving fermentation, aerobic stability, and ruminal digestibility of high-moisture sorghum silage.
Chemical and Physical Quality, Fermentation Characteristics, Aerobic Stability, and Ruminal Degradability of Sorghum Silage Inoculated with Lactiplantibacillus plantarum and Limosilactobacillus fermentum Fitriani, D.; Ardiansyah, M.; Kurniawati, A.; Bachruddin, Z.; Paradhipta, D. H. V.
Tropical Animal Science Journal Vol. 47 No. 4 (2024): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2024.47.4.483

Abstract

This study was carried out to determine the effect of homo (Lactiplantibacillus plantarum FNCC 0020) and hetero (Limosilactobacillus fermentum BN21) fermentative lactic acid bacteria on chemical compositions, fermentation characteristics, aerobic stability, and ruminal digestibility of sorghum (Sorghum bicolor L. Moench) silage. The sorghum forage was harvested at the milk ripening phase with a dry matter content of 25.6% and fermented for 100 days with different inoculants: treatments without inoculant (CON), L. plantarum (LP), L. fermentum (LF) as well as a mixture of LP and LF at a ratio of 1:1 (MIX). The experiment was conducted using a completely randomized design with 5 replications per treatment, and all inoculants were applied at 105 cfu/g of fresh forage. The results showed that LF silage caused a 66.3% reduction in cyanide acid content, the lowest mold count, and longer aerobic stability compared to LP and CON. The lowest pH (p<0.05) and highest organic matter digestibility (p<0.05) were obtained on LP silage, while the CON silage showed no significant difference. The LP and LF silage showed the highest total volatile fatty acid (p<0.05), while there was no significant between CON and others. The LF silage had the highest acetate and the lowest propionate (p<0.05). These results showed that L. fermentum was more effective in decreasing cyanide acid content and increasing the aerobic stability of sorghum silage, while L. plantarum was able to lower pH and reduce ammonia concentration.
Co-Authors C. T. Noviandi Fitriani, D. Kusmayadi, A. M. Ardiansyah M. Hidayat N.R. Dewi Nasution, M. D. M. Paradhipta, D. H. V. Rahman, M. M. S. Sundari Umami, N. W. Isnaeni Wati, A. K. Z. Bachruddin Zuprizal (Zuprizal)