Article Per Year (5 Year)
p-Index From 2020 - 2025
1.431
P-Index
This Author published in this journals
All Journal Biosaintifika: Journal of Biology & Biology Education BioEksakta : Jurnal Ilmiah Biologi Unsoed
Dyah Fitri Kusharyati
Fakultas Biologi Universitas Jenderal Soedirman Purwokerto
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

Published : 8 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 8 Documents
Search

 1 
Bifidobacterium longum, a Predominant Bifidobacterium in Early-life Infant Potentially Used as Probiotic Kusharyati, Dyah Fitri; Rovik, Anwar; Ryandini, Dini; Pramono, Hendro
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.25895

Abstract

In early life, Bifidobacteria are reported as dominant bacteria in the human digestive tract. Bifidobacterium is potential as a probiotic. The probiotic property of Bifidobacterium is strain-specific. This study aimed to identify the Bifidobacterium (isolated from less than one-month-old healthy infant stool that potentially used as probiotic) based on the 16S rRNA gene and determining their similarities among Bifidobacteria. The probiotic-potentially Bifidobacterium was re-characterized by performing a Gram’s staining and catalase test. The DNA extraction process was followed by the 16S rRNA amplification using 27F-1492R primers. Sequence similarity was checked by using the BLAST program in the GenBank. The phylogenetic tree was constructed by using a neighbor-joining (NJ) method within the MEGA version 7.0 package. The 16S rRNA gene was presented at 1,500 bp length. Bifidobacterium strains have a 91.14-94.26 % sequence similarity to B. longum subsp. longum strain CCUG30698 which is considered as insufficient for species and genus identifications. However, those isolates could be assigned in a phylogenetic position. This present study suggested the B. longum as the dominant strain of Bifidobacterium in the gut of early-life infants which has potential as a probiotic and is considered as an ideal probiotic for human consumption. This study is useful as basic information for other related research, as well as its application in industrial or community service fields.
Kualitas Yoghurt dengan Penambahan Bifidobacterium sp. Bb2E Putri, Yolandina Salsabila; Kusharyati, Dyah Fitri; Pramono, Hendro
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 1 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.1.1802

Abstract

Yoghurt merupakan produk pangan fungsional hasil fermentasi dari susu yang melibatkan peran bakteri asam laktat (BAL) Lactobacillus bulgaricus dan Streptococcus thermophillus. Menurut beberapa penelitian terdapat BAL yang memiliki ketahanan lebih baik dalam saluran pencernaan manusia, yaitu Bifidobacterium sp.. Selain itu, Bifidobacterium sp. memiliki manfaat diantaranya adalah meningkatkan fungsi pencernaan, menurunkan kolestrol, sebagai antioksidan dan anti inflamasi, mengurangi gejala alergi, serta dianggap sebagai salah satu bakteri probiotik yang penting dalam tubuh manusia. Tujuan dari penelitian adalah mengetahui pengaruh penambahan Bifidobacterium sp. Bb2E terhadap kualitas yoghurt dan mengetahui konsentrasi Bifidobacterium sp. Bb2E yang terbaik dari segi organoleptik. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 3 kali ulangan, sehingga terdapat 12 unit percobaan. Perlakuan yang diterapkan adalah perbandingan konsentrasi starter yoghurt yang berbeda dari BAL L. bulgaricus, S. thermophillus dan substitusi Bifidobacterium sp. Bb2E dengan perbandingan 2:2:0 (Y0), 2:2:1 (Y1), 2:2:2 (Y2), dan 2:2:3 (Y3). Variabel dalam penelitian ini, variabel bebas, yaitu konsentrasi Bifidobacterium sp. Bb2E dan variabel terikat, yaitu kualitas yoghurt. Parameter utama adalah kadar asam laktat. Parameter pendukung, yaitu total BAL pada yoghurt, nilai pH, dan sifat organoleptik pada yoghurt. Data diolah dengan Analysis of Variance (ANOVA) dengan taraf kesalahan 5%. Penambahan Bifidobacterium sp. Bb2E tidak memberi pengaruh nyata terhadap kualitas yoghurt dari segi kadar asam laktat, pH, dan total BAL dan yoghurt dengan starter Lactobacillus bulgaricus : Streptococcus thermophillus : Bifidobacterium sp. Bb2E dengan perbandingan konsentrasi 2:2:3 merupakan yoghurt yang disukai konsumen dengan nilai rata-rata tertinggi pada uji organoleptik.
Pengaruh Penambahan Prebiotik Inulin dan Fruktooligosakarida (FOS) terhadap Pertumbuhan Probiotik Bifidobacterium sp. Bb2E Isnasari, Azma Nurizqi; Kusharyati, Dyah Fitri; Oedjijono, Oedjijono
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 3 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3385

Abstract

Human health is very closely related to the condition of the human digestive tract since the beginning of life. The diversity of microorganisms found in the human digestive tract is very diverse, consisting of 300-500 different species of bacteria to increase the working power of nutrition. Prebiotics such as inulin and FOS and probiotics such as Bifidobacteria are aspects that can be added for the increase of nutrition. Optimal bacterial growth can be seen in the bacterial growth curve. The research problems were how the effect of prebiotic inulin and FOS on the growth of Bifidobacterium sp. Bb2E, the amount of incubation time needed to support the growth of Bifidobacterium sp. Bb2E, and how the interaction between prebiotic types and incubation times on the growth of Bifidobacterium sp. BB2E. The purposes of this study were to study the effect of prebiotic inulin and FOS on the growth of Bifidobacterium sp. Bb2E, to know the optimal incubation time of Bifidobacterium sp. Bb2E, and to know the interaction between prebiotic types and incubation times on the growth of Bifidobacterium sp. BB2E. This research was conducted using a completely randomized design (CRD) with factorial patterns. The main parameter measured was the population of Bifidobacterium sp. Bb2E, and the additional parameters measured were the pH level and the value of lactic acid titrated. The independent variable discussed in this study is prebiotic estimation on the medium, while the dependent variable considered is the population of Bifidobacterium sp. BB2E. The results of this study showed that the addition of inulin and fructooligosaccharide prebiotic at different incubation times had a significant effect on the growth of Bifidobacterium sp. Bb2E. The best treatment was a combination of inulin + FOS at incubation time of 18 hours with an optical density value was 1,794 and a total population density was 2,44x1010 CFU/mL.
Penentuan Kualitas Air Waduk Cacaban, Tegal, Jawa Tengah Berdasarkan Parameter Mikrobiologi Amaliah, Fitri; Rahayu, Diana Retna Utarini Suci; Kusharyati, Dyah Fitri
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 4 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3496

Abstract

Waduk Cacaban terletak di Kecamatan Kedungbanteng Kabupaten Tegal, Jawa Tengah. Waduk Cacaban merupakan tempat dengan berbagai gangguan antropologis seperti kegiatan domestik, industri, dan kegiatan lainnya yang berdampak negatif terhadap sumberdaya air dan dapat menurunkan kualitas air. Salah satu cara untuk mengetahui kualitas perairan adalah analisis bakteri coliform. Tujuan dari penelitian ini adalah untuk menentukan kualitas air Waduk Cacaban berdasarkan parameter mikrobiologi, dan menentukan status perairan Waduk Cacaban berdasarkan Indeks Pencemaran. Metode yang digunakan pada penelitian ini adalah metode survei, sedangkan metode pengambilan sampel yaitu purposive sampling pada 3 stasiun (dermaga, inlet, dan keramba) dengan 2 kali pengambilan setiap stasiunnya. Parameter yang diamati yaitu bakteri coliform dan fecal coli dengan parameter pendukung yaitu pH, temperatur, dan Total Dissolved Solid (TDS). Analisis dilakukan berdasarkan parameter mikrobiologi sesuai standar dari SNI 06-4158-1996 dan status Waduk Cacaban berdasarkan Indeks Pencemaran sesuai KepMen LH Nomor 115 Tahun 2003 tentang Pedoman Penentuan Status Mutu Air. Perairan Waduk Cacaban memiliki temperatur berkisar antara 29,2 – 32,6˚C, TDS 137 - 156 mg/L. pH 6, total Coliform 565 – 2850 CFU/100 mL, dan fecal coli 0 – 425 CFU/100 mL. Status pencemaran pada dermaga Waduk Cacaban (inlet, dermaga, dan keramba) dalam kondisi baik dan memenuhi baku mutu.
Karakterisasi dan Optimasi Aktivitas Bakteriosin Isolat Bakteri Asam Laktat LG-90 Asal Sedimen Mangrove Pantai Logending Mayliani, Fiqita; Kusharyati, Dyah Fitri; Ryandini, Dini
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 1 (2022): Bioeksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.1.4575

Abstract

Lactic acid bacteria (LAB) are a group of Gram-positive bacteria that produce various active metabolites, including lactic acid, ethanol, hydroperoxides, and bacteriocins. Bacteriocin is a peptide that has a broad spectrum to inhibit the growth of pathogenic microbes. The ability of bacteriocins to inhibit microbial growth is influenced by various factors, including the concentration of antimicrobial substances, temperature, storage time, pH, and microbial properties. LG-90 isolated from mangrove sediments at Logending Beach located in Ayah Village, Ayah District, Kebumen Regency, is known to be capable of producing bacteriocins. This research aimed to determine the characteristics and identity of LG-90, the optimum time of bacteriocin production, and the optimum pH and temperature for bacteriocin activity of LG-90 as antimicrobial agents. This research used a survey method. The independent variable in this research was LAB LG-90 isolates and the dependent variable were the ability to produce bacteriocins and their antibacterial power. The main parameter observed was diameter of the inhibition zone and the supporting parameters were morphological, physiological, and biochemical properties of bacteria. Descriptive data analysis and characterization of bacterial isolates refers to Bergey’s Manual of Systematic Bacteriology. The results showed that LG-90 from the mangrove sediments of Logending Beach had the similar phenetic characters as the genus Lactobacillus. Optimum bacteriocin production of LG-90 at an incubation time of 16 hours. Optimum antimicrobial activity of LG-90 bacteriocin at pH 6 and heating temperature of 40oC.
Pengaruh pH dan Suhu terhadap Aktivitas Enzim Protease Isolat LG-37 Asal Sedimen Mangrove Pantai Logending Kebumen Sari, Dhea Nur Khomala; Kusharyati, Dyah Fitri; Ryandini, Dini
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 6 No 1 (2024): Bioeksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2024.6.1.4674

Abstract

Enzim protease merupakan enzim yang berfungsi menghidrolisis protein menjadi peptida dan asam amino. Setiap enzim memiliki kondisi atau aktivitas optimal berbeda-beda. Aktivitas enzim dipengaruhi oleh berbagai faktor diantaranya adalah pH dan suhu. Penelitian ini bertujuan untuk mengetahui pengaruh pH, suhu, dan kombinasi keduanya terhadap aktivitas enzim protease dari isolat LG-37 asal sedimen mangrove Pantai Logending. Metode penelitian dilakukan secara eksperimental menggunakan rancangan acak lengkap (RAL) pola faktorial dengan dua faktor. Masing-masing faktor tersebut dilakukan pengulangan tiga kali. Faktor pertama yakni variasi pH 4, 5, dan 6, sedangkan faktor kedua yakni variasi suhu 45, 50, dan 55oC. Hasil penelitian menujukkan bahwa pH dan suhu berpengaruh terhadap aktivitas enzim protease isolat LG-37 asal sedimen mangrove Pantai Logending. Aktivitas protease tertinggi diperoleh pada perlakuan kombinasi pH 6 dan suhu 50oC dengan nilai unit aktivitas protease sebesar 1,067 U/mL.
Characteristics and Antibacterial Activities of Bacteriocin Produced by Lactid Acid Bacteria Isolate LG-50 and LG-90 Putri, Thalitha Nindika Nathania; Kusharyati, Dyah Fitri; Ryandini, Dini
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 5 No 3 (2023): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2023.5.3.4721

Abstract

Biopreservative agents is natural food preservative agent derived from microbes such as bacteriocin produced by lactic acid bacteria (LAB). Bacteriocin is a protein compound that has a small molecular weight and has antibacterial activity because it is effective in preventing the growth of pathogenic bacteria in food and beverages. Escherichia coli and Staphylococcus aureus are pathogenic bacteria that can contaminate seafood products because they can cause infection and food toxication. Isolates LG-50 and LG-90 are LAB, bacterial collection of the Microbiology Laboratory, Faculty of Biology, Isolate from mangrove sediment in Logending beach. Both isolates are not yet known for their potencies to synthesize bacteriocin and its inhibitory capability to the growth E. coli and S. aureus. The research aimed to determine the ability of LG-50 and LG-90 isolates to produce bacteriocin, to determine the ability of LG-50 and LG-90 isolates to inhibit the growth of E. coli and S. aureus bacteria invitro, to identity the characteristics of isolate LG-50 and LG-90 from Logending beach mangrove sediment. The results of this research show that the bacteriocins LG-50 and LG-90 were able to inhibit the growth of E.coli with inhibition zone diameters of 13.5 mm and 13 mm, in S. aureus with inhibition zone diameters of 15 mm and 15.5 mm.
Bioremediation of Cadmium (Cd) in Batik Wastewater Using Different Carrier Media Containing Rhizobacteria Lestari, Sri; Oedjijono, Oedjijono; Santoso, Slamet; Kusharyati, Dyah Fitri; Fathurrohim, Ramadi Habib
Biosaintifika: Journal of Biology & Biology Education Vol 15, No 1 (2023): April 2023
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i1.37683

Abstract

Batik wastewater is very dangerous for the environment and health because it contains heavy metals, such as Cadmium (Cd), derived from naphthol and indigo sol dyes. Rhizobacteria have great potential to detoxify Cd on a laboratory scale. Therefore, they should be packaged in carrier media to ensure their long-term viability in the field. The carrier media used were peat, sawdust, and tofu solid waste. This research aimed to determine the most potential of three rhizobacteria as tolerant to Cd from 11 isolates, the best carrier media that can maintain rhizobacteria viability after freeze-dried, and the effectiveness of carrier media containing potential rhizobacteria in reducing Cd in batik wastewater. Furthermore, an experimental method with completely randomized and split-plot designs was used. Rb1, Rb3, and Rb6 were the most Cd-tolerant of the three rhizobacteria isolates tested. For the viability of each isolate, tofu solid waste and peat were the best carrier media at Rb, while sawdust and tofu solid waste were the best at Rb3 and peat at Rb6. Isolate Rb3C3 had the highest percentage value of degrading Cd at 85.1%, while others had less than 80%. Isolate rhizobacteria with a carrier media makes it easy to apply on a field scale because it already contains a source of nutrients for bacterial growth and the packaging has a longer shelf life.
Co-Authors Amaliah, Fitri Diana Retna Utarini Suci Rahayu, Diana Retna Utarini Suci Dini Ryandini Fathurrohim, Ramadi Habib Hendro Pramono Isnasari, Azma Nurizqi Mayliani, Fiqita Oedjijono Oedjijono, Oedjijono Putri, Thalitha Nindika Nathania Putri, Yolandina Salsabila Rovik, Anwar Sari, Dhea Nur Khomala Slamet Santoso Sri Lestari