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KEMAMPUAN ISOLAT Lactobacillus SB4l dan SB1n TERHADAP KADAR KOLESTEROL HDL & LDL TIKUS PUTIH Kusharyati, Dyah Fitri; Hendrati, P. Maria; Ryandini, Dini
Prosiding Vol 3, No 1 (2012)
Publisher : Prosiding

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Lactobacillus SB4l dan SB1n mampu menghasilkan asam laktat sehingga dapat dimanfaatkan sebagai starter yang efektif pada pembuatan jus tomat fermentatif yang berpotensi dalam menurunkan kolesterol. Kadar kolesterol total dalam darah terdiri dari Kadar High Density Lipoprotein (HDL), Low Density Lipoprotein (LDL) dan trigliserida. Penelitian ini bertujuan untuk mengetahui pengaruh dan konsentrasi efektif minuman jus tomat fermentative dengan inokulum Lactobacillus SB4l dan SB1n terhadap perubahan kadar kolesterol HDL dan LDL darah tikus putih. Perlakuan yang dicobakan pemberian pakan pada tikus putih dengan penambahan jus tomat fermentatif dengan berbagai konsentrasi, berturutturut 0 ml, 0,3 ml, 0,6 ml, dan 0,9 ml per bobot tubuh Hasil penelitian menunjukkan bahwa konsentari 0,6 ml/bobot tubuh minuman jus tomat fermentatif Lactobacillus SB4l dan SB1n merupakan konsentrasi terbaik dalam meningkatkan kadar kolesterol HDL darah tikus putih 30 mg/dl dan menurunkan kadar Kolesterol LDL darah tikus putih 27,9 mg/dl

Antibacterial Activity of Streptomyces SAE4034 Isolated from Segara Anakan Mangrove Rhizosphere against Antibiotic Resistant Bacteria Ryandini, Dini; Pramono, Hendro; Sukanto, Sukanto
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.12896

Actinomycetes SAE4034 isolates was isolated from Rhizophora apiculata rhizosphere mud showed some antibacterial properties. The antibacterial ability of this isolate has not been tested on antibiotic resistant bacterial pathogens. However, there was no research has been reported regarding actinomycetes from Segara Anakan mangrove area resulting compounds inhibit the growth of antibiotics-resistant bacteria. Therefore, it is important to investigate its capability against antibiotics resistant bacteria or multi drug resistant bacteria (MDR bacteria). The research aimed to know the ability of actinomycetes SAE4034 in inhibit MDR bacteria and to identify the species profiles. The research methods included isolate characterization involving morphology, physiology/enzymatic and molecular properties. MDR bacterial inhibition assay, antibacterial compound extraction and antibacterial compound test using thin layer chromatography (TLC) method, observation of morphological and biochemical properties, DNA isolation, amplification and analysis of 16SrRNA sequence, and phylogeny tree analysis. The methods of this study included MDR anti-bacterial assay and antibacterial compound test. Subsequent step was isolate characterization including observation of morphological and physiological / enzymatic properties, and 16S rRNA gene sequence. The results showed that culture extract was able to inhibit the growth of MDR bacteria i.e. Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterococcus sp., but no inhibition to Enterobacter cloacae. The bioactive compound showed 4 spots with Rf values of 0.36; 0.45; 0.54; and 0.6. Based on morphology, physiology / enzymatic and 16S rRNA gene sequences characteristics, actinomycetes SAE4034 isolate is Streptomyces sp. This research showed new Streptomyces strain that serves as a source of MDR antibacterial compounds and useful in development of antibiotic for combating infectious diseases caused by MDR bacteria

Bifidobacterium longum, a Predominant Bifidobacterium in Early-life Infant Potentially Used as Probiotic Kusharyati, Dyah Fitri; Rovik, Anwar; Ryandini, Dini; Pramono, Hendro
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.25895

In early life, Bifidobacteria are reported as dominant bacteria in the human digestive tract. Bifidobacterium is potential as a probiotic. The probiotic property of Bifidobacterium is strain-specific. This study aimed to identify the Bifidobacterium (isolated from less than one-month-old healthy infant stool that potentially used as probiotic) based on the 16S rRNA gene and determining their similarities among Bifidobacteria. The probiotic-potentially Bifidobacterium was re-characterized by performing a Gramâ€™s staining and catalase test. The DNA extraction process was followed by the 16S rRNA amplification using 27F-1492R primers. Sequence similarity was checked by using the BLAST program in the GenBank. The phylogenetic tree was constructed by using a neighbor-joining (NJ) method within the MEGA version 7.0 package. The 16S rRNA gene was presented at 1,500 bp length. Bifidobacterium strains have a 91.14-94.26 % sequence similarity to B. longum subsp. longum strain CCUG30698 which is considered as insufficient for species and genus identifications. However, those isolates could be assigned in a phylogenetic position. This present study suggested the B. longum as the dominant strain of Bifidobacterium in the gut of early-life infants which has potential as a probiotic and is considered as an ideal probiotic for human consumption. This study is useful as basic information for other related research, as well as its application in industrial or community service fields.

Aktivitas Antibakteri Ekstrak Tubuh Buah Coprinus comatus Menggunakan Pelarut dan Konsentrasi yang Berbeda terhadap Escherichia coli dan Staphylococcus aureus Evita, Ellen; Ratnaningtyas, Nuniek Ina; Ryandini, Dini
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 1 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.1.1989

Coprinus comatus merupakan salah satu jamur yang dapat menghasilkan senyawa bioaktif. Ekstrak dari jamur ini sering digunakan pada bidang medis, seperti anti-kanker, anti-diabetes, dan penyakit kardiovaskular, namun masih belum ada informasi sebagai anti-bakteri. Tujuan penelitian ini yaitu mengetahui jenis pelarut yang menghasilkan rendemen tertinggi dan memiliki aktivitas antibakteri lebih tinggi terhadap E. coli dan S. aureus, mengetahui pengaruh umur simplisia terhadap aktivitas anti-bakteri, mengetahui konsentrasi terendah ekstrak tubuh buah C. comatus yang mampu menghambat pertumbuhan bakteri E. coli dan S. aureus, mengetahui aktivitas penghambatan ekstrak etil asetat dan ekstrak etanol terhadap E. coli dan S. aureus. Penelitian ini diawali dengan proses ekstraksi senyawa antibakteri dari C. comatus menggunakan metode maserasi. Pelarut yang digunakan dalam proses ekstraksi yaitu pelarut etanol dan etil asetat. Pengujian aktivitas anti-bakteri ekstrak C. comatus dilakukan terhadap Escherichia coli dan Staphylococcus aureus. Uji aktivitas anti-bakteri menggunakan simplisia berumur 7 bulan yang telah disimpan di lemari pendingin dan dibandingkan dengan ekstrak dari simplisia berumur 4 minggu. Penelitian ini menggunakan metode eksperimental menggunakan rancangan penelitian Rancangan Acak Lengkap (RAL) dengan perlakuan penggunaan pelarut dan konsentrasi ekstrak yang berbeda. Hasil penelitian menunjukkan bahwa ekstrak etanol memiliki rendemen lebih tinggi dari ekstrak etil asetat, namun aktivitas penghambatan ekstrak etil asetat lebih baik terhadap kedua bakteri dibandingkan ekstrak etanol. Simplisia berumur 4 minggu menunjukkan aktivitas penghambatan yang lebih baik dibandingkan simplisia berumur 7 bulan. Konsentrasi terendah yang dapat menghambat E. coli dan S. aureus adalah konsentrasi 6,75% dari kedua ekstrak. Secara umum lebih baik terhadap S. aureus lebih peka terhadap kedua ekstrak dibandingkan E. coli.

Aktivitas Amilolitik Bakteri Sedimen Mangrove Pantai Logending, Ayah, Kebumen pada Suhu dan pH Berbeda Rahmawati, Alfiani; ., Oedjijono; Ryandini, Dini
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 4 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.4.4574

Mangrove sediment is a source of various microorganisms that degrade leaf litter or parts of mangrove plants, including amylolytic bacteria. Bacteria isolated from the mangrove sediments of the Logending beach have not yet known their amylolytic ability. Optimum amylase activity can be influenced by temperature and pH. This study aims to determine the ability of mangrove sediment bacterial isolates to produce amylase enzymes, to determine the pH and temperature for optimum amylase activity, and to identify amylase-producing bacteria. The research was conducted by survey method. The research stages included screening for amylolytic bacteria, preparation of growth curves, amylase production, optimizing amylolytic activity at various temperatures (35ºC; 36ºC; 37ºC; 38ºC) and pHs (4.5; 5; 5.5; 6) and characterization of amylolytic isolates. The parameters measured were amylolytic index, amylase activity quantitatively, total number of bacteria, and identity of amylolytic bacteria from mangrove sediments. Data analysis was done descriptively. The results showed that the highest amylolytic index were shown by isolate LG113 with an amylolytic index value of 9.86 from 10 amylolytic isolates from mangrove sediments. The optimum temperature for amylase activity was 37ºC, which was 2.13 U/mL and the optimum pH was 6, which was 2.14 U/mL. The total number of amylolytic bacterial cells at the end of the production period was 1.94.1013 cfu/mL. The amylolytic isolate LG113 belongs to Bacillus genera.

Karakterisasi dan Optimasi Aktivitas Bakteriosin Isolat Bakteri Asam Laktat LG-90 Asal Sedimen Mangrove Pantai Logending Mayliani, Fiqita; Kusharyati, Dyah Fitri; Ryandini, Dini
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 1 (2022): Bioeksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.1.4575

Lactic acid bacteria (LAB) are a group of Gram-positive bacteria that produce various active metabolites, including lactic acid, ethanol, hydroperoxides, and bacteriocins. Bacteriocin is a peptide that has a broad spectrum to inhibit the growth of pathogenic microbes. The ability of bacteriocins to inhibit microbial growth is influenced by various factors, including the concentration of antimicrobial substances, temperature, storage time, pH, and microbial properties. LG-90 isolated from mangrove sediments at Logending Beach located in Ayah Village, Ayah District, Kebumen Regency, is known to be capable of producing bacteriocins. This research aimed to determine the characteristics and identity of LG-90, the optimum time of bacteriocin production, and the optimum pH and temperature for bacteriocin activity of LG-90 as antimicrobial agents. This research used a survey method. The independent variable in this research was LAB LG-90 isolates and the dependent variable were the ability to produce bacteriocins and their antibacterial power. The main parameter observed was diameter of the inhibition zone and the supporting parameters were morphological, physiological, and biochemical properties of bacteria. Descriptive data analysis and characterization of bacterial isolates refers to Bergey’s Manual of Systematic Bacteriology. The results showed that LG-90 from the mangrove sediments of Logending Beach had the similar phenetic characters as the genus Lactobacillus. Optimum bacteriocin production of LG-90 at an incubation time of 16 hours. Optimum antimicrobial activity of LG-90 bacteriocin at pH 6 and heating temperature of 40oC.

Determination of Streptomyces sp. SAE4034 Antioxidant and Antibacterial Compound and Its Inhibitory Mechanism on Staphylococcus aureus Kurniawati, Pratiwi Kusuma; Ryandini, Dini; Hernayanti, Hernayanti
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 3 (2022): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.3.4593

The genus Streptomyces is known as producers of bioactive compounds that have antibacterial and antioxidant properties. Streptomyces sp. SAE4034 was known to produce antibacterial compounds that can inhibit and kill pathogenic bacteria, including Staphylococcus aureus, but the group of antibacterial compounds and their inhibitory mechanisms were not yet known. The ability of Streptomyces sp. SAE4034 in producing antioxidants is unknown yet. This research aimed to determine the classification of antibacterial compounds produced by Streptomyces sp. SAE4034, its potency in producing antioxidant compounds, and to observe the inhibitory mechanisms of antibacterial compounds produced by Streptomyces sp. SAE4034 against S. aureus using cell leakage method. The results of this research showed that Streptomyces sp. SAE4034 produce compounds in the groups of alkaloid, terpenoid, flavonoid, and polyphenol. Compounds with Rf values 0.40 and 0.47 which includes into polyphenol and 0.72 which includes into alkaloid group has the ability to inhibit the growth of S. aureus. The MIC value was 256 μg/mL. Streptomyces sp. SAE4034 has a very strong antioxidant activity that can scavenge DPPH free radical with IC50value of 32,14 ppm and has an antioxidant enzyme activity that is superoxide dismutase amount of 63,10 U/mL The inhibition mechanism of Streptomyces sp. SAE4034 crude extract against S. aureus was through leaking of the cell wall or cell membrane resulting in the release of nucleic acid and protein of S. aureus cells and the highest cell leakage was at 2 doses of MIC.

Pengaruh pH dan Suhu terhadap Aktivitas Enzim Protease Isolat LG-37 Asal Sedimen Mangrove Pantai Logending Kebumen Sari, Dhea Nur Khomala; Kusharyati, Dyah Fitri; Ryandini, Dini
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 6 No 1 (2024): Bioeksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2024.6.1.4674

Enzim protease merupakan enzim yang berfungsi menghidrolisis protein menjadi peptida dan asam amino. Setiap enzim memiliki kondisi atau aktivitas optimal berbeda-beda. Aktivitas enzim dipengaruhi oleh berbagai faktor diantaranya adalah pH dan suhu. Penelitian ini bertujuan untuk mengetahui pengaruh pH, suhu, dan kombinasi keduanya terhadap aktivitas enzim protease dari isolat LG-37 asal sedimen mangrove Pantai Logending. Metode penelitian dilakukan secara eksperimental menggunakan rancangan acak lengkap (RAL) pola faktorial dengan dua faktor. Masing-masing faktor tersebut dilakukan pengulangan tiga kali. Faktor pertama yakni variasi pH 4, 5, dan 6, sedangkan faktor kedua yakni variasi suhu 45, 50, dan 55oC. Hasil penelitian menujukkan bahwa pH dan suhu berpengaruh terhadap aktivitas enzim protease isolat LG-37 asal sedimen mangrove Pantai Logending. Aktivitas protease tertinggi diperoleh pada perlakuan kombinasi pH 6 dan suhu 50oC dengan nilai unit aktivitas protease sebesar 1,067 U/mL.

Characteristics and Antibacterial Activities of Bacteriocin Produced by Lactid Acid Bacteria Isolate LG-50 and LG-90 Putri, Thalitha Nindika Nathania; Kusharyati, Dyah Fitri; Ryandini, Dini
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 5 No 3 (2023): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2023.5.3.4721

Biopreservative agents is natural food preservative agent derived from microbes such as bacteriocin produced by lactic acid bacteria (LAB). Bacteriocin is a protein compound that has a small molecular weight and has antibacterial activity because it is effective in preventing the growth of pathogenic bacteria in food and beverages. Escherichia coli and Staphylococcus aureus are pathogenic bacteria that can contaminate seafood products because they can cause infection and food toxication. Isolates LG-50 and LG-90 are LAB, bacterial collection of the Microbiology Laboratory, Faculty of Biology, Isolate from mangrove sediment in Logending beach. Both isolates are not yet known for their potencies to synthesize bacteriocin and its inhibitory capability to the growth E. coli and S. aureus. The research aimed to determine the ability of LG-50 and LG-90 isolates to produce bacteriocin, to determine the ability of LG-50 and LG-90 isolates to inhibit the growth of E. coli and S. aureus bacteria invitro, to identity the characteristics of isolate LG-50 and LG-90 from Logending beach mangrove sediment. The results of this research show that the bacteriocins LG-50 and LG-90 were able to inhibit the growth of E.coli with inhibition zone diameters of 13.5 mm and 13 mm, in S. aureus with inhibition zone diameters of 15 mm and 15.5 mm.

Karakterisasi dan Pengaruh Senyawa Antibakteri Streptomyces spp. dalam Menghambat Pertumbuhan Vibrio cholerae Andani, Ajeng Putri Retno; Oedjijono, Oedjijono; Ryandini, Dini
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 5 No 4 (2023): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2023.5.4.4726

Penelitian ini bertujuan untuk mengetahui kemampuan Streptomyces spp. yang diisolasi dari sedimen mangrove dalam menghambat pertumbuhan bakteri Vibrio cholerae, mengetahui kekuatan senyawa antibakteri yang dihasilkan oleh Streptomyces spp. terhadap V. cholerae, dan mengetahui karakteristik dari senyawa antibakteri yang dihasilkan oleh Streptomyces spp. Tahap penelitian meliputi penapisan isolat bakteri Streptomyces spp. (SA34, SA37, SA40, SAE4034) yang menghambat V. cholerae, produksi senyawa antibakteri, uji aktivitas antibakteri dengan metode Kirby Bauer, penentuan nilai MIC (%), pemisahan senyawa antibakteri dengan metode TLC, uji fitokimia, uji bioautografi, uji aktivitas senyawa antibakteri pada variasi suhu (40oC, 60oC. 80oC, 100oC) dan pH (2, 4, 6, 9), serta uji konfirmasi karakter bakteri. Data dianalisis secara deskriptif. Hasil penapisan menunjukkan bahwa penghambatan terhadap pertumbuhan V. cholerae tertinggi ditunjukkan oleh isolat SAE4034. Ekstrak kasar mampu menghambat V. cholerae dengan diameter zona hambat 11,5-17,5 mm; nilai MIC 30%, dan menghasilkan senyawa bioaktif dengan nilai Rf berkisar antara 0,31-0,70. Senyawa yang mampu menghambat memiliki nilai Rf 0,43 dan senyawa tersebut termasuk golongan alkaloid. Perlakuan suhu 40oC tetap tinggi dengan zona hambat 14 mm dan aktivitas penghambatannya menurun sejalan dengan perlakuan suhu yang lebih tinggi sampai 100oC. Perlakuan pH 6 menunjukkan aktivitas penghambatan tertinggi dengan zona hambat 16 mm, sebaliknya pada pH 2, 4, dan 9 aktivitas antibakteri ekstrak kasar menurun.

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