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All Journal Majalah Kedokteran The Indonesian Journal of Infectious Diseases Acta Biochimica Indonesiana Majalah Kedokteran Andalas Journal of Clinical Microbiology and Infectious Diseases

Ardiana Kusumaningrum

Departemen Mikrobiologi Fakultas Kedokteran Universitas Indonesia

Author-ID : 303138

Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Articles

Perbandingan Efektivitas Klorheksidin 2% dalam Isopropil Alkohol 70% dengan Antiseptik Sesuai Prosedur Operasional Standar pada Persiapan Pembedahan Ardiana Kusumaningrum; Gortap Sitohang; Hindra I. Satari; Tony Loho; Firsty
Majalah Kedokteran UKI Vol. 34 No. 4 (2018): OKTOBER-DESEMBER
Publisher : Fakultas Kedokteran Universitas Kristen Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/mkvol34iss2pp60

Abstrak Untuk mencegah Infeksi daerah operasi, dilakukan persiapan kulit pasien sesaat sebelum pembedahan. Antiseptik preparasi pembedahan pada Prosedur Operasional Standar (POS) terdiri atas tiga rejimen yaitu sabun klorheksidin 4%, povidon iodin dan isopropil alkohol 70%. Oleh karena itu, perlu dilakukan penilaian efektivitas klorheksidin – isopropil alkohol sebagai kandidat bahan preparasi pembedahan yang lebih sederhana. Desain penelitian adalah studi potong lintang pada pasien operasi bersih terkontaminasi elektif <3 jam di RSUPN Dr. Cipto Mangunkusumo selama periode Juni hingga Agustus 2014. Terdapat dua kelompok perlakuan persiapan kulit yaitu menggunakan klorheksidin 2% dalam isopropil alkohol 70% dan kelompok antiseptik sesuai POS. Spesimen swab kulit diambil sebelum dan sesudah persiapan kulit, kemudian dihitung dan dibandingkan jumlah koloni bakteri. Dari 40 pasien kelompok antiseptik uji dan 42 pasien kelompok antiseptik sesuai POS menunjukkan perbedaan bermakna pada hitung koloni sebelum dan sesudah pemberian klorheksidin 2% dalam isopropil alkohol 70% (p=0,45, uji Mann Withney) dan pemberian antiseptik sesuai POS (p=0,15). Tidak terdapat perbedaan efektivitas kelompok yang mendapat klorheksidin 2% dalam isopropil alkohol 70% dibandingkan kelompok yang mendapat antiseptik sesuai POS (p>0,05). Larutan klorheksidin 2% dalam isopropil alkohol 70% sama efektifnya dengan antiseptik sesuai POS dalam preparasi kulit sebelum operasi sehingga dapat menjadi pilihan karena penggunaannya lebih sederhana dan lebih murah.Kata kunci: klorheksidin 2%, isopropil alkohol 70%, preparasi kulit, antiseptik Abstract To prevent Surgical Site Infection, skin preparation should be done before surgery. Antiseptic used in skin preparation according to Standard Operating Procedure (SOP) consists of three regimens consist of 4% chlorhexidine soap, povidone iodine and 70% isopropyl alcohol. Therefore, it is necessary to assess the effectiveness of chlorhexidine - isopropyl alcohol as a candidate for a simpler skin preparation material. The study design was a cross-sectional study in patients who were subjected to elective contaminated clean surgery <3 hours at RSUPN Dr. Cipto Mangunkusumo during June to August 2014. There were two groups of skin preparation treatments using chlorhexidine 2% in 70% isopropyl alcohol and the antiseptic group according to SOP. Skin swab specimens were taken before and after skin preparation, then bacterial colonies count were done. The results were analyzed using SPSS 20.0. Of the 82 patients who met the inclusion criteria, both SOPs antiseptic and tested antiseptic group consisted of 40 patients and 42 patients respectively. there was significant differences in colony count before and after administration of 2% chlorhexidine in 70% isopropyl alcohol (p = 0.45 and antiseptic administration according to POS (p = 0.15). There was no significant difference in the effectiveness of the group that received 2% chlorhexidine in 70% isopropyl alcohol compared to the group that received POS antiseptics (p> 0.5). It was concluded that a 2% chlorhexidine gluconate solution in 70% isopropyl alcohol was as effective as the use of three SOP regimens in skin preparation before surgery and can be an option because its use is simpler and cheaper.Keywords: 2% chlorhexidine, 70% isopropyl alcohol, skin preparation, antiseptic

PREVALENSI ISOLAT MRSA PENGHASIL PANTON-VALENTINE LEUKOCIDIN PADA PASIEN ICU RUMAH SAKIT TERSIER Linosefa Linosefa; Delly Chipta Lestari; Ardiana Kusumaningrum; Anis Karuniawati; Andi Yasmon
Majalah Kedokteran Andalas Vol 39, No 1 (2016): Published in April 2016
Publisher : Faculty of Medicine, Universitas Andalas

Penilitian ini bertujuan untuk mengetahui prevalensi MRSA penghasil Panton-Valentine leukocidin (PVL) dan pola kepekaannya. Sampel penelitian adalah isolat MRSA dari 315 pasien Rumah Sakit Umum Pusat Nasional Cipto Mangunkusumo (RSUPNCM) selama tahun 2011 dan 2014, dengan melakukan identifikasi, uji kepekaan dan uji molekuler terhadap isolat tersebut. Penelitian ini menunjukkan sebanyak 59% dari koloni MRSA yang ditemukan masih sensitif terhadap antibiotik golongan selain β-laktam, sehingga masih dapat diduga sebagai community-associated MRSA (CA-MRSA). CA-MRSA sepertinya mulai ditransmisikan di fasilitas kesehatan. Uji molekuler terhadap isolat MRSA memberikan hasil 8,3% isolat MRSA menghasilkan PVL. Berdasarkan tipe pola kepekaannya isolat MRSA penghasil PVL tersebut masih dapat digolongkan sebagai CA-MRSA. MRSA penghasil PVL ditemukan di RSUPNCM sebagai kolonisasi. Surveilan perlu dilakukan untuk memahami interaksi antara MRSA di komunitas dan rumah sakit, terutama untuk mengurangi transmisi di fasilitas kesehatan.

Deteksi Acinetobacter baumannii Multiresisten Obat Penghasil Biofilm menggunakan Pewarnaan Berbasis Crystal Violet Ardiana Kusumaningrum; Iin Maemunah; Dimas Seto Prasetyo; Fera Ibrahim; Budiman Bela
The Indonesian Journal of Infectious Diseases Vol 5, No 2 (2019): The Indonesian Journal of Infectious Disease
Publisher : Rumah Sakit Penyakit Infeksi Prof Dr. Sulianti Saroso

Pendahuluan: Kasus infeksi terkait biofilm merupakan masalah besar pada dunia kesehatan karena sifat kekebalannya terhadap antibiotik dan respon imun, terutama pada kasus infeksi kronik akibat Acinetobacter baumannii. Saat ini terdapat berbagai metode deteksi pembentukan biofilm, namun pemeriksaan tersebut belum dilakukan secara rutin karena berbagai keterbatasan. Oleh karena itu, perlu dilakukan optimasi metode deteksi biofilm menggunakan bahan yang rutin tersedia di laboratorium serta mengukur proporsi sampel yang mengandung bakteri penghasil biofilm Metode: Desain penelitian ini adalah uji eksperimental. Pada penelitian ini dilakukan pengembangan deteksi pembentukan biofilm menggunakan metoda tabung microcentrifuse tube 1,5 ml berbahan polypropylene dengan zat warna berbasis crystal violet konsentrasi 0,1% dan 1%. Optimasi yang dilakukan meliputi media biakan, lama inkubasi, inokulum bakteri yang digunakan serta bahan pembilas. Isolat bakteri Pseudomonas aeruginosa ATCC 10145, A. baumannii ATCC 19606 dan Staphylococcus aureus ATCC 25923 digunakan sebagai kontrol positif dan kontrol negatif pemeriksaan yang dilakukan. Hasil: Pemeriksaan optimasi yang dilakukan, diperoleh kondisi optimal pembentukan biofilm menggunakan media Luria Bertani dengan besar inokulum 1 sengkelit penuh, lama inkubasi 30 jam dan pewarnaan dilakukan menggunakan crystal violet 0,1% serta bahan pembilas berupa PBS steril. Proporsi pembentukan biofilm pada A. baumannii multiresisten obat sebesar 55,3%. Kesimpulan: Metode deteksi pembentukan biofilm menggunakan metode tabung polypropylene yang dimodifikasi dan pewarnaan zat warna crystal violet 0,1% merupakan metode deteksi yang mudah dikerjakan, reproducible dan efisien, sehingga dapat dilakukan di laboratorium mikrobiologi klinik sederhana. Proporsi bakteri penghasil biofilm adalah lebih dari 50% A. baumannii resisten multiobat.

The use of urine lipoarabinomannan for establishing pulmonary TB in HIV patient: case report and systematic review Margono, Djiwa; Yulianti Ningtias, Pranudiah; Kusumaningrum, Ardiana; Prasetyo, Dimas Seto
Journal of Clinical Microbiology and Infectious Diseases Vol. 3 No. 2 (2023): Available online: December 2023
Publisher : Indonesian Society for Clinical Microbiology (Perhimpunan Dokter Spesialis Mikrobiologi Klinik Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/jcmid.v3i2.38

Introduction: Indonesia is still the country that provides the second-largest estimated number of TB cases worldwide. Several patients had TB with HIV-positive. HIV-positive patients with symptoms suggestive of pulmonary tuberculosis often difficult to produce sputum of sufficient quality. Lateral flow lipoarabinomannan examination with urine specimens is expected to be easy, inexpensive, fast, and accurate point-of-care testing. The aim of this research was to assess the sensitivity and specificity of the lateral flow method in detecting lipoarabinomannan (LAM) from urine specimens in HIV-positive patients with suspected pulmonary tuberculosis. Sputum cultures were utilised as a reference standard. Case Description: A 21-year-old male patient came to the clinic complaining of a cough that had not subsided since the previous month. Cough felt throughout the day with a little white phlegm. The patient admitted to having high-risk sexual behavior and had been tested positive for HIV but had not started antiretroviral therapy. The laboratory examination results showed that the leukocyte count was 3600/mm3 and the radiological examination revealed a chest X-ray within normal limits. The patient had been tested for sputum smears with negative results but was still advised to start TB treatment. The patient has not started treatment because he feels that his sputum smear test results are negative. Currently, the patient is not willing to have his sputum checked again. The polyclinic doctor knows the lipoarabinomannan (LAM) examination that uses urine specimens to diagnose TB but does not yet know its effectiveness for this patient. Conclusion: In this case report, the patient was unwilling to do a diagnostic test related to the TB. The systematic review was that the Alere Determine™ TB LAM Ag may not be clinically applicable for diagnosing tuberculosis infection in patients living with HIV at this time. However, Fujifilm SILVAMP TB LAM® could be regarded as a supplementary tool to the established diagnostic protocol for tuberculosis in HIV-positive patients.

Detection of latent tuberculosis infection in household contacts of drug-resistant tuberculosis patients using interferon-gamma release assay: a study at Universitas Indonesia Hospital Indratmo, Muhammad Faris; Handayani, Diah; Kusumaningrum, Ardiana; Iswanti, Febriana Catur; Sadikin, Mohamad
Acta Biochimica Indonesiana Vol. 8 No. 2 (2025): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.214

Background: Drug-resistant tuberculosis (DR-TB) poses significant public health challenges in Indonesia. Household contacts of DR-TB patients face elevated risk of Mycobacterium tuberculosis infection, which may remain latent and asymptomatic. Objective: This study aimed to assess the prevalence of latent tuberculosis infection (LTBI) among household contacts of DR-TB patients using interferon-gamma release assay (IGRA). Methods: This cross-sectional study was conducted at Universitas Indonesia Hospital from February to May 2023. Eighteen asymptomatic household contacts from six confirmed DR-TB index cases were enrolled. Participants underwent clinical evaluation, chest radiography, and LTBI screening using the QuantiFERON-TB Gold Plus (QFT-Plus) assay. Results: Among 18 participants (mean age 33.3 years; 55.6% female), 8 (44.4%) tested positive for LTBI, while 10 (55.6%) tested negative. The highest IGRA positivity rates were observed in adolescents aged 12–16 years (66.7%) and young adults aged 17–25 years (60.0%). All participants were clinically asymptomatic with normal chest radiographs. Conclusion: This study demonstrates substantial LTBI prevalence among household contacts of DR-TB patients. The findings underscore the importance of systematic contact tracing, IGRA-based screening, and timely tuberculosis preventive therapy to reduce disease transmission and progression in high-risk populations.

Co-Authors Andi Yasmon Anis Karuniawati Budiman Bela Delly Chipta Lestari Diah Handayani Dimas Seto Prasetyo Febriana Catur Iswanti Fera Ibrahim Firsty Gortap Sitohang Hindra I. Satari Iin Maemunah Indratmo, Muhammad Faris Linosefa Linosefa Margono, Djiwa Mohamad Sadikin Tony Loho Yulianti Ningtias, Pranudiah

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