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All Journal Forum Pasca Sarjana Biosaintifika: Journal of Biology & Biology Education
Bibiana Widyawati Lay
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Agriculture, Biological Sciences & Forestry Education

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Carotenoid Production by Rhodosporidium paludigenum Using Orange Peel Extract as Substrate Warjoto, Renna Eliana; Jennifer, Jennifer; Lay, Bibiana Widyawati
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.24867

Abstract

Carotenoids are fat-soluble pigments that have various benefits in health and beauty. The demand and market of carotenoids are increasing; thus, the faster and cheaper biological production of these pigments using microorganisms is desired. In this study, the optimum medium pH and nitrogen concentration for carotenoid production by Rhodosporidium (R.) paludigenum using orange peel extract as substrate were determined. The soluble sugars from orange peels were extracted using distilled water at 100 °C. The yeast inoculum was cultivated in Yeast Peptone Dextrose (YPD) media before fermentation. The independent variables in the fermentation factorial design included initial medium pH (5; 6; and 7) and nitrogen concentration (0; 1; and 1.75 g/L urea in the medium). The decrease in reducing sugar concentration and the increase in biomass dry weight during fermentation demonstrated carbon source consumption by R. paludigenum for growth and carotenoid production. On the sixth day of fermentation (D6), the culture was harvested for the total carotenoid extraction and determination. The highest total carotenoid yield (107.63 µg/g) was achieved by the treatment group with an initial medium pH of 6 without nitrogen supplementation. This result indicated the orange peel extract potential as a substrate for carotenoid production using R. paludigenum as a workhorse. This was the first reported research in natural carotenoid generation using R. paludigenum (strain InaCC Y-236) locally isolated in Indonesia as the producer and orange peel extract as the substrate. Thus, this research would enrich the microbial carotenoid exploration and waste valorization to value-added products at the same time. The study can then be developed further and upscaled for industrial applications. 
The purpose of this research is to built a water quality model which explains DO-BOD responsse as the effect of organic loading by hydrodynamic behavior, chemical and biological processes in the stream which are developed mathematically from reoxygenation rate (ka), deoxygenation rate (kd), photosynthesis and respiration, sediment oxygen demand (SOD) that are got from laboratory analysis and direct measuring in the field. The research was done in laboratory using standard method and also done in Widyo Astono; Muhammad Sri Saeni; Bibiana Widyawati Lay; Soepangat Soemarto
Forum Pasca Sarjana Vol. 31 No. 1 (2008): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

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Abstract

The purpose of this research is to built a water quality model which explains DO-BOD responsse as the effect of organic loading by hydrodynamic behavior, chemical and biological processes in the stream which are developed mathematically from reoxygenation rate (ka), deoxygenation rate (kd), photosynthesis and respiration, sediment oxygen demand (SOD) that are got from laboratory analysis and direct measuring in the field. The research was done in laboratory using standard method and also done in field when rainy and dry season. Parameters that were measured are water temperature, pH, BOD, DO, discharge, velocity, and water depth. Based on the research, DO-BOD responsse observation has a same tendency with the result of model.   Key words: hydrodinamic, reoxygenation rate, decomposition rate, DO-BOD responsse
Co-Authors Jennifer Jennifer Muhammad Sri Saeni Soepangat Soemarto Warjoto, Renna Eliana Widyo Astono