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All Journal Jurnal Penelitian Pertanian Terapan Biosaintifika: Journal of Biology & Biology Education MITRA: Jurnal Pemberdayaan Masyarakat Indonesian Journal of Natural Pigments Jurnal Bakti Masyarakat Indonesia Bioscientist : Jurnal Ilmiah Biologi
Warjoto, Renna Eliana
Program Studi Sarjana Bioteknologi, Fakultas Teknobiologi, Universitas Katolik Indonesia Atma Jaya
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Immunology & microbiology Public Health Social Sciences Other

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Carotenoid Production by Rhodosporidium paludigenum Using Orange Peel Extract as Substrate Warjoto, Renna Eliana; Jennifer, Jennifer; Lay, Bibiana Widyawati
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.24867

Abstract

Carotenoids are fat-soluble pigments that have various benefits in health and beauty. The demand and market of carotenoids are increasing; thus, the faster and cheaper biological production of these pigments using microorganisms is desired. In this study, the optimum medium pH and nitrogen concentration for carotenoid production by Rhodosporidium (R.) paludigenum using orange peel extract as substrate were determined. The soluble sugars from orange peels were extracted using distilled water at 100 °C. The yeast inoculum was cultivated in Yeast Peptone Dextrose (YPD) media before fermentation. The independent variables in the fermentation factorial design included initial medium pH (5; 6; and 7) and nitrogen concentration (0; 1; and 1.75 g/L urea in the medium). The decrease in reducing sugar concentration and the increase in biomass dry weight during fermentation demonstrated carbon source consumption by R. paludigenum for growth and carotenoid production. On the sixth day of fermentation (D6), the culture was harvested for the total carotenoid extraction and determination. The highest total carotenoid yield (107.63 µg/g) was achieved by the treatment group with an initial medium pH of 6 without nitrogen supplementation. This result indicated the orange peel extract potential as a substrate for carotenoid production using R. paludigenum as a workhorse. This was the first reported research in natural carotenoid generation using R. paludigenum (strain InaCC Y-236) locally isolated in Indonesia as the producer and orange peel extract as the substrate. Thus, this research would enrich the microbial carotenoid exploration and waste valorization to value-added products at the same time. The study can then be developed further and upscaled for industrial applications. 
Carotenoid Production by Rhodosporidium paludigenum Using Cassava Starch Hydrolyzed by Bacillus subtilis as Substrate Warjoto, Renna Eliana; Felianti, Felianti; Lay, Bibiana Widiyati
Indonesian Journal of Natural Pigments Vol 2 No 2 (2020): Agustus 2020
Publisher : Ma Chung Research Center for Photosynthetic Pigments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33479/ijnp.2020.02.02.36

Abstract

Carotenoids are natural pigments with colors ranging from yellow to red that are beneficial for food, cosmetics, and animal feed industries. These pigments can be found in fruits, vegetables, algae, and microorganisms. Among all microorganisms that have been known to produce carotenoids, Rhodosporidium paludigenum is still poorly investigated. Therefore, this study aimed to determine the potential of carotenoid production by R. paludigenum using cassava starch hydrolyzed by Bacillus subtilis as a substrate. The cassava starch for hydrolysis was divided into four concentrations, i.e., 2%, 4%, 6%, and 8% w/v. During the hydrolysis period, the amylase enzyme activity produced by B. subtilis was evaluated. The reducing sugar concentration was then examined to determine the optimum medium for carotenoid production. The highest amylase enzyme activity was produced on the second day in all cassava starch concentrations. However, the highest reducing sugar concentration was discovered in the 6% w/v cassava starch concentration. Thus, a batch submerged fermentation for carotenoid production by R. paludigenum was performed using the hydrolysate as the sole substrate. At the end of the fermentation, the total carotenoid was extracted, and the concentration was determined using spectrophotometry. The total yield of xanthophyll over biomass was higher than that of β-carotene. These findings elucidated the potency of cassava starch hydrolysate obtained from the starch hydrolyzed by B. subtilis, for carotenoid production by the red yeast R. paludigenum.
Pengaruh Media Tanam Hidroponik terhadap Pertumbuhan Bayam (Amaranthus sp.) dan Selada (Lactuca sativa) Renna Eliana Warjoto; Tati Barus; Joshua Mulyawan
Jurnal Penelitian Pertanian Terapan Vol 20 No 2 (2020)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jppt.v20i2.1610

Abstract

As the human population rises, the demand for high nutritional foods, such as vegetables, also increases. However, agricultural land, especially in urban areas, is narrowing. Hydroponics can be one of the solutions to answer this problem. In addition to complete nutrition, hydroponic plants also require appropriate media for root attachment, water absorption, and aeration. Unfortunately, no studies have compared the growth of hydroponic plants using rockwool and sponge as affordable growing media. Therefore, this study aimed to determine the effect of local rockwool, imported rockwool, and sponge as growing media on the growth of spinach  (Amaranthus sp.) and lettuce  (Lactuca sativa) using Deep Flow Technique (DFT). This study used a Completely Randomized Design with three treatment groups, i.e., three different growing media, for spinach and lettuce. At the harvest period, the number of leaves on each plant was counted, the crown fresh-weight was balanced, and the chlorophyll content was determined. Plant height was also measured for spinach. The results showed that the three types of growing media had no significant effect on the number of leaves, plant height, crown fresh-weight, and chlorophyll content of spinach. However, the number of leaves  (25.25 ± 1.38) and the crown fresh-weight  (141.27 ±16.49 g) of lettuce grown on the local rockwool media were significantly higher than the number of leaves (17.00 ± 0.77) and the crown fresh-weight (81.48 ± 9.50 g) of lettuce planted on the sponge media. Other hydroponic growing media’s influences can be investigated for further research, with additional growth parameters such as crown dry- weight, root fresh- and dryweight, as well as the determination of plant phytochemical contents. Moreover, the correlation between nutritional formulations and the type of growing media can be further examined to obtain the most optimum spinach and lettuce growth.
Produksi Karotenoid oleh Rhodosporidium paludigenum dalam Media Yeast Peptone Dextrose dengan Suplementasi Molase Renna Eliana Warjoto; Theresia Andriana; Bibiana Widiati Lay
Bioscientist : Jurnal Ilmiah Biologi Vol 9, No 2 (2021): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v9i2.4338

Abstract

Carotenoid pigments are commonly found in fruits and vegetables, but carotenoids can also be produced by microorganisms such as yeasts. Carotenoids have several benefits, including antioxidant compounds and vitamin A precursors. Production of carotenoids by microorganisms depends on various factors, such as pH, temperature, and nutrient content in the media. This study aimed to determine the optimum concentration of molasses supplementation and the initial pH of the media for carotenoid production by the red yeast Rhodosporidium paludigenum. In this study, the red yeast was cultivated in Yeast Peptone Dextrose (YPD) media supplemented with molasses, with a total sugar concentration of 2% in the media. This study had a factorial design with eight treatment groups, i.e., molasses supplementation of 0%, 25%, 50%, and 75% in the media with initial pH of 5 and 6 for each molasses supplementation. The results showed that the culture in the media with supplementation of 25% molasses and pH 5 produced the highest levels of carotenoids when compared to those with 50% and 75% molasses supplementation concentrations. The yields of β-carotene and xanthophyll over biomass dry-weight in the media with 25% molasses supplementation at pH 5 were 26.67 μg/g and 31.15 μg/g, respectively. Nevertheless, the red yeast in the media without supplementation (0%) produced the highest carotenoid concentration among all treatments.
Bokashi Fertilizer Production from Waste Processing at Rusunawa Muara Baru Renna Eliana Warjoto; Meda Canti; Anastasia Tatik Hartanti; Benedicta Evienia Prabawanti
MITRA: Jurnal Pemberdayaan Masyarakat Vol 4 No 2 (2020): MITRA: Jurnal Pemberdayaan Masyarakat
Publisher : Institute for Research and Community Services

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25170/mitra.v4i2.1069

Abstract

Waste that is not managed well may potentially cause diseases and negatively affect the quality of life. Rusunawa Muara Baru, Jakarta, is one of our partner communities that need continuous training in all aspects, including waste management. Therefore, the present community service program was carried out to educate the residents of Rusunawa Muara Baru on the importance of sorting waste and how to process household organic waste into liquid organic fertilizer and bokashi compost. The program was conducted twice in two weeks. At the first meeting, an introductory questionnaire was distributed, an interactive discussion was performed, and a fertilizer production workshop using simple composters was initiated. Two weeks later, the composting results were evaluated, and the participants filled out the evaluation questionnaire. Approximately 83% of the participants agreed to sort household waste after the program was completed, which showed the participants' increasing awareness in waste processing. The liquid biofertilizer produced was used for planting vegetable seeds even though it still had a foul odour. The quality of the compost produced, however, was still below expectations. We suggest that in the future, education and workshops on simple household-scale composting methods capable of producing good quality liquid organic fertilizer and compost without odour should be conducted.
PENINGKATAN KESADARAN LINGKUNGAN BAGI PENGURUS ORGANISASI SISWA INTRA-SEKOLAH: PEMBUATAN PUPUK ORGANIK CAIR DARI LIMBAH Renna Eliana Warjoto; Tati Barus
Jurnal Bakti Masyarakat Indonesia Vol 4, No 1 (2021): Jurnal Bakti Masyarakat Indonesia
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Tarumanagara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24912/jbmi.v4i1.9605

Abstract

The waste problem in Indonesia has not yet been entirely resolved. The biggest challenge is the lack of waste management awareness in various walks of life. Therefore, we should start the environmental education and waste management awareness as early as possible in families and schools. This activity aimed to train senior high school students, especially the student organization boards, to recycle organic waste into liquid organic fertilizer. The student organization boards are leadership figures at the school. Therefore, through this activity, they were expected to set a good example for other students related to organic waste management and valorization at school. The activity was initiated by an interactive lecture, continued by a liquid organic fertilizer processing workshop and an interactive discussion. At the time of the harvest, one group of participants succeeded in producing liquid organic fertilizer without a foul odor. In general, the participating students stated that liquid organic fertilizer was reasonably easy to produce even though it was not as simple as composting. For future attempts, the combination of activities including organic waste conversion into liquid organic fertilizer or compost and inorganic waste processing into handicraft creations will be more interesting for students. In the long-term point of view, the awareness of the high school students in organic as well as inorganic waste management is expected to increase and becomes a positive impact on the broader environment and communitiesABSTRAK: Persoalan sampah di Indonesia belum sepenuhnya terselesaikan. Tantangan terbesar adalah kurangnya kesadaran tentang pengelolaan sampah di berbagai lapisan masyarakat. Oleh karena itu, kita perlu memulai pendidikan lingkungan dan kesadaran tentang pengelolaan sampah sedini mungkin dalam keluarga dan sekolah-sekolah. Kegiatan ini bertujuan melatih siswa sekolah menengah atas (SMA), khususnya para pengurus Organisasi Siswa Intrasekolah (OSIS) untuk mendaur ulang sampah organik menjadi pupuk organik cair (POC). Para pengurus OSIS adalah figur siswa yang menjadi pemimpin organisasi di sekolah. Oleh karena itu, melalui kegiatan ini diharapkan mereka dapat menjadi contoh atau teladan yang baik bagi siswa lainnya dalam pengelolaan dan pemanfaatan sampah organik di lingkungan sekolah. Kegiatan ini diawali dengan ceramah interaktif yang kemudian dilanjutkan dengan lokakarya pembuatan POC dan diskusi interaktif. Pada saat panen, satu kelompok peserta berhasil memperoleh POC tanpa bau yang tidak sedap. Secara umum, siswa peserta menyatakan bahwa pembuatan POC cukup mudah dilakukan meskipun tidak sesederhana pembuatan kompos. Untuk kegiatan serupa di masa yang akan datang, kombinasi aktivitas terkait konversi sampah organik menjadi POC dan kompos yang tidak berbau serta pengolahan sampah anorganik menjadi aneka kreasi kerajinan tangan akan lebih menarik bagi para siswa. Dalam jangka panjang, diharapkan kesadaran para siswa dalam mengolah dan memanfaatkan kembali sampah organik maupun anorganik dapat meningkat dan menjadi dampak positif bagi lingkungan masyarakat yang lebih luas.
PENYEDIAAN SARANA CUCI TANGAN SEDERHANA UNTUK PENCEGAHAN PENYEBARAN COVID-19 DI PASAR GENTENG, SURADITA, CISAUK Listya Utami Karmawan; Widya Agustinah; Renna Eliana Warjoto; Daru Seto Bagus Anugrah; Danniella Yudianti; Illene Nanine; Eunice Catherine Witness
Jurnal Bakti Masyarakat Indonesia Vol 4, No 1 (2021): Jurnal Bakti Masyarakat Indonesia
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Tarumanagara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24912/jbmi.v4i1.9487

Abstract

COVID-19, a source of distress in most countries, has encouraged world leaders to issue necessary policies to resolve this global pandemic. An alarming rate of infections in the community has pushed the Indonesian government to come up with the Large-Scale Social Restriction (Pembatasan Sosial Berskala Besar/PSBB). The faculty of Biotechnology of Atma Jaya Catholic University of Indonesia has since initiated a community service to halt the spread of COVID-19 by supplying simple hand washing facilities around Pasar Genteng Traditional market in Suradita, Cisauk. Prior to the installations, the traditional market lacked proper sanitizing facilities. Clean water sources such as taps are commonly situated far away from the marketplace. Traders and visitors crowd these areas each day, exponentially increasing the risk of spreading COVID-19. The crowded populations of the traditional market are a motivating factor for this community service, which aims to increase the public awareness in the prevention of COVID-19. Activities of this community service includes installations of hand washing facilities along with educational infographics of proper sanitizing procedures. A total of 21 participants attended the community service while maintaining physical distancing conditions and were encouraged to use the hand wash facilities at the end of the service. The community service was successful, providing impactful benefits towards the traders and visitors, with a raised community awareness of the importance of personal hygiene. The facilities are well maintained after 4 weeks and exemplary community awareness is displayed from their initiative that showed from the monitoring and evaluation results.  ABSTRAK:COVID-19, yang merupakan sumber penyebab keresahan berbagai negara di dunia telah menyebabkan pemerintah dunia mengeluarkan kebijakan untuk mengatasi pandemi yang terjadi secara global ini. Data di Indonesia menunjukkan angka terinfeksi yang terus meningkat sehingga pemerintah mengeluarkan kebijakan berupa Pembatasan Sosial Berskala Besar (PSBB). Oleh karena itu, Fakultas Teknobiologi Universitas Katolik Indonesia Atma Jaya berinisiatif melakukan kegiatan pengabdian masyarakat untuk menghambat tingkat penyebaran COVID-19 melalui penyediaan sarana cuci tangan sederhana di Pasar Genteng. Pasar tradisional ini terletak di kompleks Suradita, Cisauk. Sebelum kegiatan pemasangan, pasar ini kekurangan fasilitas sanitasi yang layak. Sumber air bersih seperti air sumur terletak jauh dari pasar dan belum ada akses air ledeng. Setiap hari pedagang dan pengunjung memadati pasar sehingga meningkatkan risiko penyebaran COVID-19. Oleh karena itu, kegiatan pengabdian masyarakat ini difokuskan pada pembuatan dan pemasangan fasilitas cuci tangan sederhana yang dilengkapi dengan infografis tentang cara mencuci tangan yang benar serta pembagian masker kain. Serah terima perangkat cuci tangan dan acara pembukaan dihadiri oleh 21 orang peserta dengan tetap memperhatikan protokol kesehatan. Kegiatan pengabdian masyarakat melalui penyediaan sarana cuci tangan sederhana di Pasar Genteng, Cisauk telah berlangsung dengan baik dan dirasakan manfaatnya oleh para pedagang dan pengunjung pasar. Sarana cuci tangan sederhana ini diharapkan dapat meningkatkan kesadaran masyarakat tentang pentingnya menjaga kebersihan diri dalam rangka pencegahan penyebaran COVID-19. Sarana cuci tangan tersebut masih dalam kondisi baik setelah empat minggu dan kesadaran masyarakat juga cukup baik yang terlihat dari hasil monitoring dan evaluasi.
Co-Authors Anastasia Tatik Hartanti Anugrah, Daru Seto Bagus Benedicta Evienia Prabawanti BIBIANA W LAY Bibiana Widiati Lay Bibiana Widyawati Lay Danniella Yudianti Eunice Catherine Witness Felianti, Felianti Illene Nanine Jennifer Jennifer Joshua Mulyawan LISTYA UTAMI KARMAWAN Meda Canti Tati Barus Theresia Andriana WIDYA AGUSTINAH