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L. A. Lesmana
Division of Hepatology, Department of Medicine, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo Hospital, Jakarta.
Medicine & Pharmacology

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Endoscopic management of difficult common bile duct stones Lesmana, L. A.
Medical Journal of Indonesia Vol 8, No 4 (1999): October-December
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1102.47 KB) | DOI: 10.13181/mji.v8i4.720

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[no abstract available]
Genotyping of hepatitis B virus from dried and stored serum on filter paper Gani, Rino A.; Handayu, A. D.; Mulatsih, Reny; Lesmana, L. A.; Sulaiman, Ali; Akbar, Nurul
Medical Journal of Indonesia Vol 14, No 4 (2005): October-December
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (149.253 KB) | DOI: 10.13181/mji.v14i4.198

Abstract

HBV genotype has aclose association with prognosis and therapy as well as for epidemiology study. However, this examination can be done only in large cities that are not practical to send serum sample due to geographical burden and facilities. The aim of this study is to know whether HBV genotype can be determined from dried and stored serum on filter paper and compare the result with sera drawn directly from chronic hepatitis B (CHB) and hepatoma patients. Twenty-three serum samples were obtained from CHB patients. HBV DNA were quantitatively determined with Cobas Amplicor HBM (Roche Diagnostics GmBH, Germany) and dropped on to 3 x 1 cm filter papers. After allowed to dry in a plastic clip, it were put in a closed envelope then stored for 1 week in room condition (27 – 33 oC). DNA extraction were done from the filter papers after a short incubation period and HBV genotypes were determined with PCR and specific primers. For comparison, 20 CHB-Hbe(+) samples and 29 hepatoma samples were drawn directly and not dried. HBV genotype were detected in 18/23 (78.2%) from dried serum samples on filter paper while in sera that were not stored, from CHB-HBe(+) samples, 20/20 (100%) could be determined while from hepatoma patients, 24/29 (82.7%) samples. The proportion of genotype were in line with other reported HBV genotype examination for Indonesia. It is concluded that detection of HBV genotype can be done from dried serum in filter paper and stored for 1 week. (Med J Indones 2005; 14: 215-9)Keywords: Hepatitis B virus, genotype, filter paper
Seroepidemiology and risk factors of Hepatitis B and C virus infections among drug users in Jakarta, Indonesia Gani, Rino A.; Budihusodo, Unggul; Waspodo, Agus; Lesmana, L. A.; Hasan, Irsan; Akbar, Nurul; Noer, H. M.S.
Medical Journal of Indonesia Vol 11, No 1 (2002): January-March
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (845.285 KB) | DOI: 10.13181/mji.v11i1.51

Abstract

The number of drug users is markedly increased in recent times. Data were collected consecutively in Cipto Mangunkusumo Hospital and Mitra Menteng Abadi Hospital in Jakarta. HBsAg were examined using reverse passive hemaglutination assay (RPHA) and anti-HCV with dipstick method; both were from the laboratoium Hepatika, Mataram, Indonesia. In a 5 month period (March - August 1999) there were 203 cases of drug users. Most of them were male ( 185 cases or 91.1%) with a mean age of 21.2 ± 4.3 years. Mean age in starting to use the drug was 18.8 ± 4.0 years. The prevalence of anti-HCV and HBsAg positivity were 74.9% (151 cases) and 9.9% (19 cases), respectively. The prevalence of double infection was 7.4% (15 cases). Injection drug users (IDU) were 168 cases (84%). Extramarital sex was done by 62 cases (30.5%), but only 16 cases (8%) with more than one partner. Tattoo was found in 32 cases ( 15.8%). Multivariate analysis revealed that lDU and tattoo were the risk factors for anti-HCV positivity, with the OR of 9.15 (95% CI 3.28-5.53) and 13.24 (96% CI 1.6 - 109.55), respectively. No significant medical risk factor could be identified for HBsAg positivity. Double infection of HBV and HCV was found in 15 cases (7.4%). We concluded that the prevalence of HBV, HCV infection and double infection of HBV - HCV in drug users were high, with tattoo and injection drug usage as risk factors for hepatitis C virus infection. (Med J Indones 2002; 11: 48-55)Keywords: HBsAg, Anti-HCV, tattoo, injection drug users
Management of biliary ascariasis: report of 21 cases Lesmana, L. A.
Medical Journal of Indonesia Vol 7, No 2 (1998): April-June
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (561.022 KB) | DOI: 10.13181/mji.v7i2.786

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[no abstract available]
Endoscopic management of bile leaks after laparoscopic cholecystectomy Lesmana, L. A.
Medical Journal of Indonesia Vol 7, No 3 (1998): July-September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (526.325 KB) | DOI: 10.13181/mji.v7i3.773

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[no abstract available]
Co-Authors A. D. Handayu Agus Waspodo Ali Sulaiman Aru W. Sudoyo Bambang Setiyohadi David R.S. Samosir H. M.S. Noer Irsan Hasan Loli Simanjuntak Marcellus Simandibrata Nurul Akbar Reny Mulatsih Rino A. Gani Unggul Budihusodo