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Endang G Lestari

Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111

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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance

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REGENERASI TUNAS DARI KALUS YANG TELAH DIBERI PERLAKUAN IRADIASI PADA PADI VARIETAS FATMAWATI [Shoot Regeneration of the Fatmawati Rice Variant Radiated Callie] Yunita, Rossa; Lestari, Endang G; Dewi, Iswari S
BERITA BIOLOGI Vol 11, No 3 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Gamma ray mutative induction for increasing genetic variation has been applied for plant prime variety engineering. The materials are derrived from seed organ, shoot and calli. Calli is a group of actively dividing cell and have not been organized to form plant. The benefit of using calli explant is that the gamma ray could directly shot to DNA in the nuclear cell in such a way that there is higher opportunity for genetic change to occur. The problema of using calli explant are the difficulties in regenerating the calli into shoots, due to the deformation as a result of radiation process. Therefore, this research is aimed at obtaining the appropriate media formulation for shoot regeneration from Fatmawati-rice calli which has been irradiated with gamma ray. The reseach was conducted in BB-Biogen laboratory consisting of three experiments, such as : (1) calli iradiation with the dosage of 0; 5; 10; 15; 20; 25; 30; 35; 40; 45; 50; 55 and 60 Gy, (2) shoot regeneration at the MS + BA (0, 1, dan 3 mg/l) + IAA (0 dan 0,8 mg/l) media, and BA (0, 1, and 3 mg/l) + zeatin ( 0; 0,1; 0,2 and 0,3 mg/l) + IAA 0,8 mg/l and (3) Shoot induction at MS + IBA (0, 1, 2 and 3 mg/l) media. The result shows that the range of LD50 was obtained at the dosage of 30 Gy, the most apropriate media for shoot regeneration is MS + BA 3 mg/l + IAA 0,8 + zeatin 0,1 mg/l and media for root induction is IBA 1 mg/l.

INDUKSI KERAGAMAN SOMAKLONAL DENGAN IRADIASI SINAR GAMMA DAN SELEKSI IN VITRO KALUS PISANG RAJABULU MENGGUNAKAN ASAM FUSARAT, SERTA REGENERASI DAN AKLIMATISASI PLANTLET Lestari, Endang G; Purnamaningsih, R; Mariska, I; Hutami, Sri
BERITA BIOLOGI Vol 9, No 4 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Pisang raja bulu is one of the most important bananas in Indonesia. However, this plant has low toleranee to wilt disease, caused by Fusarium oxysporum f. eubense. Its mass cultivation is inhibited by the absence of variety tolerant to the disease.A wide range of genetic variability will be needed if selection for novel characters is to be conducted, especially when there is no source of resistance gene available for breeding materials. This research consisted of callus induction from primary explant, induction of somaclonal variation using gamma iradiation, and in vitro selection using fusaric acid, followed by regeneration and acclimatization of selected plantlets. The media applied for callus induction was MS (Murashige and Skoog. 1962) + 2,4-D I and 3 mg/l + NAA 0 and 0.1 mg/l and 2,4-D 5 mg/l + BA 0.5 mg/l + Casein hidrolysate (CH) 500 mg/l. The applied gamma irradiation dosage were 0, 5.0. 7.5. 10 and 15 Gy. The irradiated calli was subsequently subcultures on selection media i.e.. MS containing fusaric acid at 30 and 45 mg/l. The living calli was then regenerated on media containing BA, TDZ. with or without proline and arginine. In addition. MS+ kinetin 5 mg/l + 1AA 0,2 mg/l was applied for shoot development. The result showed that the most suitable callus induction media for pisang raja bulu was MS +2.4-D 5 mg/l +BA 0.5 mg/l +CH 500 mg/l. The gamma irradiation of 10 Gy produced somaclone lines which were able to proliferate bud nodules on selection media containing fusaric acid at 30 and 45 mg/l. The media used for shoot development was MS + kinetin 5 mg/l + IAA 0,2 mg/l. Planllet obtained from the in vitro were then successfully acclimatized in the green house.

INDUKSI MUTASI DAN SELEKSI IN VITRO MENGGUNAKAN ASAM FUSARAT UNTUK KETAHANAN PENYAKIT LAYU PADAPISANG AMBON HIJAU Lestari, Endang G; Mariska, I; Roostika, I; Kosmiatin, M
BERITA BIOLOGI Vol 8, No 1 (2006)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Due to its high vitamin and nutrition content, banana (Musa paradisiaca L.) is deemed necessary as the mineral resources. The demand on the disease free seedlings are recently increasing. However, facing the problems of Fusarium attack in the production centers, health seedlings in the sufficient number are difficult to be provided. Hence, to solve the problems, mutative induction and in vitro selection to the shoot tip explants has been carried out in banana cv "Ambon Hijau", This research was conducted at the tissue culture laboratory of Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development. On level of 500; 750; 1000; and 1500 rad, Gamma ray radiation have been applied, continued by in vitro selection by using 0, 30 and 45 mg/1 fusaric acid. The selected explant about 0,5 cm were treated for 2 x 4 week selection period. The result showed that the best medium for regeneration was MS basal medium contains 3 mg/1 BA. The irradiation could increase somaclonal variation as well as created some new somaclones that resistant to fusaric acid. However irradiation and in vitro selection caused inhibition of culture growth. The more dosage of irradiation and concentration of fusaric acid decreased regeneration rate of explant. Inoculation by using conidia (5 g/kg soil) provided 18 putative mutant and higher concentration of conidia (lOg/lOkg soil) produced 37 mutant that resistant to Fusarium.

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