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All Journal Nexus Biomedika Nexus Kedokteran Translasional Syifa' MEDIKA: Jurnal Kedokteran dan Kesehatan
Endang Listyaningsih
Faculty of Medicine, Sebelas Maret University
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Public Health

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Pengaruh Pemberian Ekstrak Buah Naga Putih (Hylocereus undatus) terhadap Kerusakan Histologis Sel Ginjal Mencit (Mus musculus) yang Diinduksi Parasetamol Christ Haryadi, Caesaria; Listyaningsih, Endang; Murti, Bhisma
Nexus Biomedika Vol 1, No 1 (2012): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: White dragon fruit (Hylocereus undatus) contains antioxidant substances in the form of vitamin A, C, E, and polyphenol that are reported to be nephroprotective. This study was aimed to verify the effect of white dragon fruit extract and its increasing doses toward paracetamol-induced damage of renal cell of mouse (Mus musculus). Methods: This study was a Randomized Controlled Trial in design. Samples were twenty eight Swiss webster type mice divided into 4 groups randomly, being seven mice in each group. Aquades were administered to mice in control group (KK) and the first group (KP1), while white dragon fruit extract were administered to the second group (KP2) and the third group (KP3) with the dose of 70 mg/20 g BB weight and 140 ml/20 g weight for 14 days, respectively. Paracetamol were administered to KP1, KP2, KP3, with dose 5.07 mg/ 20 g weight on day 12th, 13th and 14th. On day 15th, mice were sacrificed with neck dislocation. Renal cell slides were made and stained by HE. Renal histological is analysed and scored base on quantification of renal histological damage. Data were analyzed by One-Way ANOVA test and Post Hoc test. Results: One-Way ANOVA showed that there was a significant difference between groups. Result of LSD method showed that there were significant of differences between KK-KP1, KK-KP2, KP1-KP2, KP1-KP3 and KP2-KP3 groups, respectively; while no significant different was found between KK-KP3. Conclusion: The administration of white dragon fruit extract might prevent the mice renal cell damage. The increasing doses of white dragon fruit extract induce protection effect towards the renal cell damage of mice induced by paracetamol Key words : white dragon fruit extract, paracetamol, renal cell damage.
Efek Perlindungan Susu Kedelai (Glycine max) Ultra High Temperature (UHT) Terhadap Lambung Mencit Wijnaputri, Ginong Pratidina; Listyaningsih, Endang; Subandono, Jarot
Nexus Biomedika Vol 1, No 2 (2012): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: White Soybean (Glycine max) Milk Ultra High Temperature (UHT) is potential to protect gaster because it contains flavonoid. This study aimed to investigate the protective effect of white soybean milk UHT on mice’s gaster due to aspirin. Higher dose of the milk reduces the damaged on gastric mucous induced by aspirin Methods: This was a labroratory experimental research with posttest only controlled group design conducted in in Histology Laboratory of Sebelas Maret University. The samples were thirty five mice divided into 5 groups. Negative control group (KN) was given normal food and aquadest, positive control group (KP) was given aspirin dose 2,275 mg/20g Body weight (W), dan first treatment group (P1) was given cimetidine dose 0,78 mg/20g W second treatment group (P2) and third treatment group (P3) was given soybean milk UHT dose 0,7ml/20 g W and 1,4 ml/20 g W. All treatments for KN, KP, P1 , P2, P3 was given in 10 days. Aspirin was given to KP, P1 , P2, P3 with dose 2,275 mg/20g weight of mice on day 8, 9 and 10. to evaluate the  level of damage based on histologic appearance, gastric staining was performed using Haem eos. Results: Kruskal-Wallis test result showed significant result among four groups. Mann-Whitney showed significant result for KN compared to KP,P1,P2 , P3 and KP compared to P1, P2 and P3 ; while P1 compared to P2, P3 and P2 compared to P3 had no significant result. Conclusion: White Soybean (Glycine max) Milk Ultra High Temperature(UHT) protect mice’s gastric from gastritis. However, higher dose of White Soybean (Glycine max) Milk Ultra High Temperature(UHT) does not increase the protective effect of white soybean milk UHT on mice’s gaster due to aspirin. Keywords: White Soybean (Glycine max) Milk Ultra High Temperature(UHT), gastric protection, gastritis, aspirin. 
Efek Nefroprotektor Ekstrak Daun Kemangi (Ocimum sanctum) Terhadap Kerusakan Sel Ginjal Mencit (Mus musculus) Yang Diinduksi Parasetamol Basith, M Abdul; Listyaningsih, Endang; Handayani, Selfi
Nexus Biomedika Vol 1, No 2 (2012): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Today, consuming paracetamol as an analgesic antipyretic drugs has  increased. Paracetamol which used in inappropriate dose has bad effect to our body, such as nefrotoxic. Basil leaf extract has antioxidant as a protection of free radicals and reducing NAPQI which produced by paracetamol. The objectives of this research are to know the nefroprotector effect and this research will shown the multilevel dose of basil leaf extract as a nefroprotector in the renal cell damage induced by parasetamol. Methods: This was experimental laboratory with the post test only controlled group design. Sample group consisted of male mice Swiss Webster, 2-3 month, + 20 g. Samples divided into 4 groups, each group has seven mice. Mice for control group (K) and the first treatment group (P1) were given aquades for 14 days. The second treatment group (P2) will be given basil leaf extract dose I for 14 days. The third treatment group (P3) will be given basil leaf extract dose II for 14 days. Paracetamol will be given to P1, P2, and P3, with dose 0,1 ml/20 gr weight of mice on the day 12, 13, and 14. Finally on day 15th, mice are sacrificed with neck dislocation then the renal of mice was made preparations with paraffin blocks methods and Hematoxillin Eosin staining. Renal cell damage observed and counted a mount of scored on renal histological karyopyknosis, karyorrhexis, and karyolysis. Data are analized by One-Way ANOVA test (α= 0,05) and continued by Post Hoc Multiple Comparisons test (LSD) (α = 0,05). Results: Result of One-Way ANOVA shown that there was a significant of degree between 4 groups. Result of LSD method there was a significant of degree between K-P1, K-P2, P1-P2, and P1-P3 groups; and also it wasn’t a significant of degree between K-P3 and P2-P3. Conclusions: The basil leaf extracts was able to decrease the renal cell damaging of mice and the increase of basil leaf extracts dose followed by the increase of protection effect to the renal cell damaging of mice which is induced by paracetamol.   Keywords: basil leaf extract, paracetamol, renal cell damage. 
Efek Pemberian Sari Buah Belimbing Manis (Averrhoa carambola Linn.)terhadap Kerusakan Histologis Hepar Tikus Putih(Rattus norvegicus) yang Diinduksi Parasetamol Cahyaningrum, Handayani Putri; Listyaningsih, Endang; Handayani, Selfi
Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Star fruit (Averrhoa carambola Linn.) juice contains flavonoids, epicatechins, proanthocyanidins, and tanins that can protect liver from free radicals. The objectives of this research were to know the effect of star fruit (Averrhoa carambola Linn.) juice to liver histological damage of albino rat induced by paracetamol. Methods: This was laboratory experimental research with post test only controlled group design. Samples were 28 male rats from Wistar type, 2-3 months old and + 200 g of each weight. Samples were divided into 4 groups of 7 rats each. Sampling technique used in this research was incidental sampling. The control group (K) and the first treatment group (P1), rats were administered aquadest for 14 days. The second treatment group (P2), rats were administered star fruits juice with the concentration of 50% 2ml/200 g body weight of rat and the third group (P3), rats were administered star fruits juice with the concentration of 100% 2ml/200 g body weight of rat for 14 days. Paracetamol was administered to groups of P1, P2, and P3 on the 12th, 13th, and 14th day. On the 15th day, rats were sacrificed and their livers were made for preparation with paraffin block method which were stained HE. Liver histological features were assessed based on quantifying of pyknosis, karyorhexis, and karyolysis. Data were analyzed using the One-Way ANOVA test (α = 0.05) and continued with Post Hoc Multiple Comparisons (LSD) test (α = 0.05). Results: Statistically, the One-Way ANOVA test showed that there were significant differences of liver damage score between K – P1, K – P2, K – P3, P1 – P2, P1 – P3, and P2 – P3. Conclusion: Star fruit juice decreased the liver histological damage of albino rat (Rattus norvegicus) induced by paracetamol. Key words: star fruit juice, paracetamol, liver histological damage, albino rat 
Efek Proteksi Biji Mahoni (Swietenia mahagoni Jacq.) terhadap Kerusakan Hepar Mencit yang Diinduksi Parasetamol Endahfuri, Dahniar; Listyaningsih, Endang; Sari, Yulia
Nexus Kedokteran Translasional Vol 1, No 1 (2012): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Background: Seedof Swietenia mahagoni Jacq. contains alkaloids, saponins, flavonoids, triterpenoids, steroids, and tannins which were thought to protect liver from free radicals.In present study, Swietenia mahagony Jacq.seed extract was evaluated for its protective effect on paracetamol-induced liver damage in mice. Methods:To evaluate the hepatoprotective effects of Swietenia mahagony Jacq. seed extract, 28 male Swiss webster micewere equally divided into four groups. Animals of group I (K) and group II (P1) were given aquadest for 14 days. Group III (P2) received 14 mg/25 g body weight of mice of Swietenia mahagony Jacq. seed extract and the last group (P3)received 28 mg/25 g body weight of mice of Swietenia mahagony Jacq. seed extract for 14 days.Paracetamol was given to groups of P1, P2, andP3.Mice were sacrificedon the 15th day and histological preparation was made to evaluate histological damage on liver. Liver histological features were assessed by counting the number of hepatocyte on centrolobuler zone undergoing pyknosis, karyorhexis, and karyolysis. Data were analyzed using the OneWay ANOVA test (α = 0.05) and then were continued with Post Hoc Multiple Comparisons (LSD) test (α = 0.05). Results:The mean liver histological damage score was significantly higher on group of P1=60.71+7.521, whilegroup of K= 9.86+1.574 as the less.P2grouphad fewer numbers of liver histological damage (32.57+2.573) compared to the P3group(43.57+3.101). The results of OneWay ANOVA and LSD test showed that there was significant difference between the four groups (p = 0.000). Conclusions:Swietenia mahagony Jacq.seed extract showed protective effect against the hepatotoxicity induced by paracetamolin mice.Swietenia mahagony Jacq.seedextract with the dose of 14 mg/25 g bodyweight of mice showed a higher hepatoprotective effect than the dose of 28 mg/25 g body weight of mice. Keywords:Swietenia mahagony Jacq. seed, liver damage,paracetamol
Gambaran Histologis Sel Ginjal Mencit (Mus musculus) yang diinduksi Parasetamol ditambahkan Ekstrak Daun Kemangi (Ocimum sanctum) Basith, Abdul; Listyaningsih, Endang; Handayani, Selfi
Syifa'Medika Vol 7, No 1 (2016): Syifa' MEDIKA: Jurnal Kedokteran dan Kesehatan
Publisher : Faculty of Medicine

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32502/sm.v7i1.1388

Abstract

Penggunaan parasetamol sebagai obat analgetik antipiretik dewasa ini semakin meningkat. Parasetamol yang digunakan dengan dosis berlebih dapat menyebabkan efek nefrotoksik. Ekstrak daun kemangi (Ocimum sanctum) mengandung antioksidan yang dapat menangkal radikal bebas dan mengurangi terbentuknya NAPQI yang dihasilkan metabolisme parasetamol. Penelitian ini bertujuan untuk mengetahui gambaran histologis sel ginjal mencit (mus musculus) yang diinduksi parasetamol dan penelitian ini untuk mengetahui efek peningkatan dosis dari ekstrak daun kemangi dapat mengurangi kerusakan sel ginjal mencit yang diinduksi parasetamol. Sampel berupa mencit jantan, galur Swiss webster berumur 2-3 bulan, + 20 gr. Sampel sebanyak 28 ekor dibagi dalam 4 kelompok, masing-masing kelompok terdiri dari 7 ekor mencit. Kelompok kontrol (K) dan kelompok perlakuan 1 (P1), mencit diberi aquades selama 14 hari. Kelompok perlakuan 2 (P2), mencit diberi ekstrak daun kemangi dosis I selama 14 hari. Kelompok perlakuan 3 (P3), mencit diberi ekstrak daun kemangi dosis II. Parasetamol dosis 0,1 ml/ 20 grBB mencit diberikan pada kelompok P1, P2, dan P3 pada hari ke-12, 13, dan 14. Hari ke-15, mencit dikorbankan kemudian ginjal mencit dibuat preparat dengan metode blok parafin dan pengecatan Hematoksilin Eosin (HE). Kerusakan sel ginjal diamati dan dinilai dari gambaran histologis berupa penjumlahan inti piknosis, karioreksis, dan kariolisis. Kerusakan sel ginjal tertinggi pada kelompok P1 (parasetamol) dengan jumlah 75.43 ±5.192 dan kerusakan paling sedikit berada di kelompok K (aquades) dengan jumlah 16.43 ± 2.637.Jumlah kerusakan pada kelompok P2 (dosis I + paracetamol) is 21.29 ± 2.289 dan jumlah kerusakan pada kelompok P2 (dosis II + paracetamol) is 18.29 ± 2.563. Ekstrak daun kemangi dapat mengurangi kerusakan sel ginjal mencit dan peningkatan dosis dari ekstrak daun kemangi dapat mengurangi kerusakan sel ginjal mencit yang diinduksi parasetamol.
Co-Authors Aviriani, Setyaningrum Bhisma Murti Caesaria Christ Haryadi Dahniar Endahfuri Dian Rachmawanti Affandi, Dian Rachmawanti Ginong Pratidina Wijnaputri Handayani Putri Cahyaningrum Jarot Subandono M Abdul Basith Selfi Handayani Sri Handajani Tamaulina Br Sembiring Yundari, Yundari