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All Journal Indonesian Journal of Cancer Chemoprevention ANNALES BOGORIENSES Makara Journal of Technology
Puspa Dewi Narrij Lotulung
Research Center for Chemistry, Indonesian Institute of Sciences, Kampus LIPI Gd. 50, Jl. Sangkuriang Bandung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Electrical & Electronics Engineering Engineering Immunology & microbiology Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology

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Identification of a New Compound as α-Glucosidase Inhibitor from Aspergillus aculeatus Dewi, Rizna Triana; Suparman, Asep; Mulyani, Hanny; Darmawan, Akhmad; Lotulung, Puspa Dewi Narrij
ANNALES BOGORIENSES Vol 20, No 1 (2016): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ab.v20i1.213

Abstract

Terestrial fungi are of great importance as potential sources of pharmaceutical agent. Aspergillus aculeatus, a fungus isolated from soil sample collected in Indonesia, was cultured in liquid media to investigate a novel compound as inhibitor α-glucosidase. The mycelium extract of A. aculeatus shows potential activity against Saccharomyces cereviseae α-glucosidase and mild activity against mammalian α-glucosidase with IC50 values of 9.57 µg/mL and 470.76 mg/mL, respectively. Enzyme assay-guided fractionation of this extract afforded rubrofusarin (1). Rubrofusarin, a linear naphtho-γ-pyrone, is a natural pigment from Aspergillus sp. Interestingly, compound 1 shows potential inhibitory activity against mammalian α-glucosidase (IC50 of 92.7 µg/mL), but no to S. cereviseae α-glucosidase. The results suggest that A. aculeatus is a promising natural source as a lead compound in the discovery of antidiabetic drug.
Production of Lovastatin and Sulochrin by Aspergillus Terreus Using Solid State Fermentation Dewi, Rizna Triana; Artanti, Nina; Mulyani, Hani; Lotulung, Puspa Dewi Narrij; Minarti, Minarti
Makara Journal of Technology Vol. 15, No. 1
Publisher : UI Scholars Hub

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Abstract

Lovastatin is an anti-cholesterol agent that was produced by Aspergillus terreus using solid state fermentation (SSF). During this fermentation process, sulochrin is also produced as an unwanted co-metabolite. However, our previous result showed that sulochrin had potential as antidiabetes because it is an inhibitor agent of α-glucosidase. In this paper, we reported our observation on lovastatin and sulochrin production pattern in relation with inhibitor α-glucosidase activity during eleven days fermentation of A. terreus koji (SSF) ethyl acetate extract. Koji obtained from solid state fermentation with rice as the substrate and incubated at room temperature, sample is taken daily for eleven day (D-1 to D-11). Lovastatin and sulochrin production was measured by Liquid Chromatography- Mass Spectrometer based on their molecular weight m/z 404.5 and 332.3 respectively. The inibitory activity is measured by inhibition model of koji extract against α-glucosidase (EC 3.2.1.20) from Saccharomyces cereviceae. The results show that lovastatin production was started on the day 2 (0.04 mg/g) and achieving the optimal production on day 7 (11.46 mg/g), while sulochrin production was started on day 4 (0.60 mg/g) and keep produced until the end of fermentation period at Day 11 (3.11 mg/g). Koji extract was started to show inhibitory to α-glucosidase activity on Day 5 (IC50= 23.34 μg/mL) and keep showed activity until Day 11 (IC50=3.33 μg/mL). These results suggest that inhibitory activity of koji extract to α- glucosidase activity have relation with sulochrin biosynthesis production.
Revelation of New Compound from Ethanolic Extract of Fragaria x ananassa var. Lembang Andayani, Desak Gede Sri; Lotulung, Puspa Dewi Narrij; Sulaswaty, Anny; Qaanitaati, Nur; Andini, Desak Gede Tirta; Mulyani, Rahmaniar; Nursyifa, Eva
Indonesian Journal of Cancer Chemoprevention Vol 10, No 3 (2019)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev10iss3pp159-168

Abstract

Fragaria x ananassa (strawberry) is a subtropical plant that can adapt well in tropical highlands. Fragaria x ananassa have been widely used to cope with health problems. The active compound component of secondary metabolites contained in Fragaria x ananassa has the potential as an antioxidant. This research is done to isolate secondary metabolites from extract of Fragaria x ananassa fruits. Extract Fragaria x ananassa was produced by maceration using ethanol as the solvent. Separation and isolation compound were carried out using Vacuum Liquid Chromatography (VLC) and Gravity Column Chromatography (GCC) guided by Thin Layer Chromatography (TLC) using hexane: ethyl acetate (3:7) as the eluent. The flavonoid compound was determined by the total content of phenolic and flavonoid in extract of Fragaria x ananassa fruits. The results of total phenolic content and total flavonoid content were 0.1130 mg/g and 0.0112 mg/g, respectively. The alkaloid compound was determined by Dragendorff testing. The elucidation of the structure by Fourier Tansform Infrared (FTIR), Nuclear Magnetic Resonance (NMR), and Liquid Chromatography Mass Spectrometry (LCMS) showed that the active compound contained in the secondary metabolite of extract ethanol from Fragaria x ananassa is 3-Cyclopentyl-5-(1-hydroxyethyl)-1,6-dihydro-7H-pyrazolo[4,3- d]pyrimidin-7-one.Keywords: Fragaria x ananassa extract, flavonoid, alkaloid, total phenolic and flavonoid content, FTIR, NMR, LCMS.
Co-Authors A.A. Ketut Agung Cahyawan W Akhmad Darmawan Andayani, Desak Gede Sri Andini, Desak Gede Tirta Anny Sulaswaty, Anny Hani Mulyani, Hani Minarti Minarti Mulyani, Hanny Mulyani, Rahmaniar Nina Artanti Nursyifa, Eva Qaanitaati, Nur Rizna Triana Dewi