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Nursamsi Pusposendjojo
Fakultas Pertanian Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry

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Ketahanan Beberapa Jamur Patogen terhadap Fungisida Christanti Sumardiyono; Nursamsi Pusposendjojo; Sri Trisnowati
Jurnal Perlindungan Tanaman Indonesia Vol 1, No 1 (1995)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (7811.105 KB) | DOI: 10.22146/jpti.9350

Abstract

Chemical control of plant pathogens have been done for a long time using contact and systemic fungicides. Resistance of the pathogens to fungicides may caused failure of disease control program. Studies by in vitro, in green house and fields were done at Yogyakarta to know the fungal which were resistant to several fungicides after treatment. The pathogen tested to fungicides were Colletotrichum capsici on red pepper to propineb, mancozeb, mixture of carbendazim and mancozeb, and thiophanate methyl, Alternaria porri on garlic to thiophanate methyl, Phytophthora palmivora on cocoa to Al-fosetyl, metalaxyl, mancozeb, and Copper Oxychloride, and Peronosclerospora maydis on corn to metalaxyl. The study indicated that C. capsici was resistant to propineb, mancozeb, and mixture of carbendazim and mancozeb, but sensitive to thiophanate methyl. A. porri from plant treated with thiophanate methyl indicated resistant to the fungicides. ED50 of Al-fosetyl and metalaxyl on P. palmivora were higher than on mancozeb and copper oxychloride, so that it was resistance strain. 
Patogenisitas Rhizoctonia solani setelah Penyimpanan pada Substrat Berbeda Nursamsi Pusposendjojo
Jurnal Perlindungan Tanaman Indonesia Vol 5, No 1 (1999)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (9807.468 KB) | DOI: 10.22146/jpti.9959

Abstract

Rhizoctonia solani Kühn is a polyphagus fungus causing damping off on nurseries. The fungus develops a resistant structure on plant debris in the form of sclerotium. The sclerotium is the major inoculum for next crops. Evidence indicated that viability and pathogenicity of the inoculum were affected by type of substrate in which the inoculum survived and by length of resting period. Rice straw appeared to be a better substrate for R. solani survival than that of soil. All seedlings planted on soil inoculated with inoculum from rice straw showed greater disease intensity, earlier symptom development, and more growth retardation than those of being inoculated with inoculum from soil.
Ekstraksi, Karakterisasi dan Daya Penghambatan Kitosan Alami terhadap Jamur Colletotrichum musae secara In Vitro Tunjung Pamekas; Christanti Sumardiyono; Nursamsi Pusposendjojo; Didik Indradewa
Jurnal Perlindungan Tanaman Indonesia Vol 15, No 1 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11764

Abstract

In order to enhance food safety, the use of chemical pesticides on agriculture must be minimized, especially on postharvest processing. One of the alternative ways is the use of natural chitosan. Chitosan has good prospect as an alternative compound to chemical pesticides to be used on postharvest processing for agriculture products which are potential as exported commodity, such as banana cv. Ambon Curup, exotic fruit from Bengkulu. The aims of this research were to extract and characterize chitosan and to evaluate the inhibition of chitosan to Colletotrichum musae. Methods of experiment were (1) extraction of chitosan, (2) characterization of chitosan, and (3) inhibition test of chitosan to the pathogen in vitro. The parameters observed were the growth and colour of colony, the measurement and density of conidia and the dry weight of mycelium. The result indicated that the texture of chitosan were flake-powder with white-brownish colour, had a little bit odor of fish and pH were 7.5–13. The deacetylated degree of chitosan were around 74.27–75.64%. The in vitro test indicated that the kind of chitosan treatments gave no significant effect to the growth and colony colour, the measurement and density of conidia and the dry weight of mycelium. However the concentration of chitosan treatments were significantly effect all parameters. The chitosan on 20 mg/ml could inhibit the colony growth up to 81.20%, the conidial density up to 46.5%, the measurement of conidia up to 19.4%, and the dry weight of mycelium up to 59.46%. Chitosan had no effect to the colour of fungal colony. 
Identifikasi Jamur Genus Aspergillus pada Gaplek di Kabupaten Gunung Kidul H. A. Oramahi; Christanti Sumardiyono; Nursamsi Pusposendjojo; Haryadi Haryadi
Jurnal Perlindungan Tanaman Indonesia Vol 12, No 1 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11959

Abstract

The study was carried out in Gunung Kidul Regency of Yogyakarta special. Dried cassava was taken from the district of Nglipar (north zone), Wonosari (center zone), Semanu and Rongkop (south zones) for identification of the genera of Aspergillus growing on the samples. Clasification of the zones was based on the degree of rainfall on the area. Dichloran 18% Glyserol Agar (DG-18) medium was used for isolating pathogen, while Czapek Yeast Extract Agar (CYA), Czapek Yeast Extract Agar with 20% sucrose (CYA 20S) and Malt Extract Agar (MEA) media were used for identification. The fungi were identified on the basis of both macroscopic and microscopic morphologies. The result showed that The Genera Of Aspergillus growing on dry cassava were A. flavus, A. niger, A. oryzae, A. foetidus, A. zonatus and A. tamarii. A. flavus was a predominant fungus that grow on dry cassava.
Eksplorasi, Pengujian, dan Identifikasi Khamir Antagonis terhadap Patogen Antraknos (Colletotrichum lagenarium) pada Semangka Kardi Raharjo; Christanti Sumardiyono; Nursamsi Pusposendjojo; Sismindari Sismindari
Jurnal Perlindungan Tanaman Indonesia Vol 12, No 1 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11964

Abstract

Phyllosphere is one of habitat of yeast and the other microorganism included an antagonistic microorganism. The antagonistic yeasts have been isolated from watermelon phyllosphere on YM Agar medium. There are 30 isolates of yeast have been found. The pure culture of virulent isolate 115 of C. lagenarium used for testing the antagonistic ability of yeast isolates. Two yeast isolates namely K10 and K15 have highest ability to inhibit growth and development of C. lagenarium colony. From microscopic observation, the isolates K10 and K15 caused deflated on C. lagenarium hyphe. The result of identification of K10 is Candida sp. and K15 is Sirobasidium sp.
Karantina Tumbuhan di Indonesia dalam Masa Perdagangan Bebas Nursamsi Pusposendjojo
Jurnal Perlindungan Tanaman Indonesia Vol 11, No 1 (2005)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12116

Abstract

Plant quarantine is enacted to keep out foreign plant pests and to protect food, horticulture and estate crops from alien pests. The act prohibits or restricts entry into or passage through a country from foreign countries of plants, plant products, soil, and other materials carrying or likely to carry pests not known· to be established in the country. Experienced inspectors stationed at all points of entry into the country enforce quarantine of produce likely to introduce new pests. The importance of plant quarantine becomes more and more significant in the free trade era. Indonesia to be one of potential market for many agricultural products has still very limited number of plant quarantine inspectors. Number and quality of plant quarantine inspectors are badly needed in order enable coping the problems. Education and training should be organized and it is important to include plant quarantine in the Faculty of Agriculture curriculum.
Co-Authors Christanti Sumardiyono Didik Indradewa Haryadi Haryadi Hasan Ashari Oramahi Kardi Raharjo Sismindari Sismindari Sri Trisnowati Tunjung Pamekas