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All Journal INDONESIAN JOURNAL OF LEGAL AND FORENSIC SCIENCES Medical Journal of Indonesia Journal of Medical Physics and Biophysics Majalah Kesehatan Pharmamedika
Djaja Surya Atmadja
Department Of Forensic Medicine And Medicolegal, Faculty Of Medicine, University Of Indonesia
Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Health Professions Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Physics Public Health

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Analisis Pola dan Dimorfisme Seksual Sidik Bibir pada Populasi Indonesia Barat Windrianto, Mochamad Atmaji; Atmadja, Djaja Surya; Yuni, Mindya
Majalah Kesehatan Pharmamedika Vol 11, No 1 (2019): JUNI 2019
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33476/mkp.v11i1.950

Abstract

Sidik bibir merupakan suatu pola berupa fissure pada permukaan mukosa bibir yang bersifat unik, stabil. Dimorfisme seksual sidik bibir ditemukan pada beberapa populasi di dunia seperti populasi di India dan Thailand. Secara fenotif, populasi Indonesia terutama populasi Indonesia Barat berbeda dengan populasi lain di dunia, sehingga tidak menutup kemungkinan terdapat dimorfisme seksual sidik bibir. Tujuan dari penelitian ini untuk mengetahui pola dan dimorfisme seksual sidik bibir pada populasi Indonesia Barat. Sampel penelitian ini total berjumlah 477 orang, yang terbagi atas 265 laki-laki dan 212 perempuan. Metode pemeriksaan yang digunakan adalah metode Suzuki dan Tsucihasi, dengan membagi bibir menjadi 8 kuadran. Hasil penelitian menunjukkan laki-laki dan perempuan pada populasi Indonesia Barat memiliki frekwensi tipe sidik bibir paling dominan type 1’, dan terdapat dimorfisme sidik bibir yang signifikan pada type 3 dan type 4 pada laki-laki dan type 1 pada perempuan. Sidik bibir merupakan salah satu metode identifikasi yang dapat dipakai untuk identifikasi ras dan jenis kelamin. Populasi Indonesia Barat memiliki pola sidik bibir dominan tipe 1’  yang berbeda dengan populasi lainnya di dunia.
Composition of Human Bone Mineral by FTIR and Its Relationship to the Age Djarwani S. Soejoko; Yessie Widya Sari; Setia Utami Dewi; Nurizati Nurizati; Kiagus Dahlan; Djaja Surya Atmadja
Journal of Medical Physics and Biophysics Vol 1, No 1 (2014): Inaugural Issue
Publisher : Indonesian Association of Physicists in Medicine (AIPM/AFISMI)

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Abstract

Deproteinated human bone with hydrazine indicated that percentage of bone mass mineral increased with individual age in the range of child to adult, and then slowly decreased with ageing. Type of bone and sex also influenced the proportional of mineral in bone. Several information was obtained from infrared spectroscopy measurements. Calcium phosphate in bone mineral was a mixture of amorphous calcium phosphates and apatite crystals that rich of carbonates. Most crystals were carbonate apatite type B, with additional small amount of type A and AB. The splitting factor of υ4 phosphate bands indicated that crystalline degree was regulated by age, increases in the range of child to adult, then decrease up to a certain value (0.16) and finally almost constant. It is predicted that apatite crystals that was formed with less calcium phosphate will accompanied by the insertion of crystallization water in order to maintain the constancy of bone volume.
MUTATION OF STR IN PATERNITY TESTING Djaja Surya Atmadja; Evi Untoro
Indonesian Journal of Legal and Forensic Sciences (IJLFS) Vol 1 (2008): Indonesian Journal of Legal and Forensic Sciences
Publisher : Penerbit, sejak 2012 : Asosiasi Ilmu Forensik Indonesia dan UPT Lab. Forensik Sain dan Kriminilogi - Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/10.24843/IJLFS.2008.v01.i01.p05

Abstract

Since the founding of DNA fingerprint by Alec J Jeffreys in 1985, DNA analysis was widely appliedin paternity testing. Nowadays, Short Tandem Repeats (STR) is the most popular DNA typing for paternitytesting because of its high discrimination power, especially when the typing is performed in combination of 6,9, 13 or 15 STR loci. STR is the nuclear DNA, and inherited from the mother and father according toMendelian law. Every child has a pair of DNA fragment, one inherited from the mother (maternal fragment),and the other from the father (paternal fragment). In paternity testing we compare the DNA typing of the childand mother to find the maternal fragment. The other fragment of the child must be the paternal fragment. Thispaternal fragment of the child, then is compared to the fragments of alleged father. The result of thiscomparison is either match (the paternal fragment is the same as one of the alleged father’s DNA fragments)or exclusion (the paternal fragment is not the same with any of the alleged father’s fragments). A child IS thebiological child of alleged father if in every STR locus the comparison is match. A child IS NOT thebiological child of alleged father if in 2 or more STR loci the comparisons are exclusion. Single exclusion in apaternity testing, that still be a single exclusion after additional STR loci analysis is usually caused bymutation. Mutation on STR locus will causes the repeat of a person shift one step more or less than theoriginal. In the case of mutation, the paternity index will decrease although we still confirm that the allegedfather is the biological father of the child. In this paper we report 2 paternity cases that showed mutation inSTR typing.
THE USAGE OF THE VOLUNTARY CADAVER IN EDUCATION OF MEDICINE THROUGH SILENT MENTOR PROGRAM Djaja Surya Atmadja; Evi Untoro
Indonesian Journal of Legal and Forensic Sciences (IJLFS) Vol 2 (2012): Indonesian Journal of Legal and Forensic Sciences
Publisher : Penerbit, sejak 2012 : Asosiasi Ilmu Forensik Indonesia dan UPT Lab. Forensik Sain dan Kriminilogi - Universitas Udayana

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Abstract

In the medical education the usage of animals, cadavers or patients for medical training is common practice all over the world. It is widely accepted that the use of human body is better than animal.  The usage of cadavers for training in medical procedures and operations is more acceptable because it causes less harm than practicing directly to the patients. The cadavers for training are usually the unknown persons. Since the source of the unknown cadavers are decreasing from time to time, nowadays some Medical Faculties is looking for alternatives, such as the persons who voluntarily want to donate their bodies for medical education, known as Silent Mentor Program. In this program the live donor candidate (Silent Mentor) writes a will, in which  the donor states that he/she want to donate the body for teaching anatomy, training on medical intervention and surgery, research, organ transplantation or even for museum preparation. This program introduces firstly in Hua Lien, Republic of China (Taiwan) and now has been widely accepted by the community. In Indonesia, Silent Mentor is a new concept. Faculty of Medicine University of Indonesia has begun to adopt this system by sending some lecturers to learn about the Program. An healthy Indonesian male, 60 years old, Moslem, is the first SM candidate in Indonesia. He has signed a will in the front of his lawyer. Now there are about 20 SM candidates, who stated that they want to join Silent Mentor Program, but now is still waiting for preparation the administrative as well as legal procedures. In Indonesia, application of SMP needs some modification, due to differences in the culture, religion, facility and regulation
DNA quality and quantity in adipose tissue: a comparison of the effects of bomb explosion Leonardo; Sugiharto, Ade Firmansyah; Indriatmi, Wresti; Atmadja, Djaja Surya; Yudianto, Ahmad; Herkutanto; Widodo, Wahyu
Medical Journal of Indonesia Vol. 32 No. 4 (2023): December
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13181/mji.oa.247206

Abstract

BACKGROUND Adipose tissue is often overlooked in DNA testing due to misconceptions about its DNA content. However, its shock-absorbing qualities may be useful for high-pressure scenarios like bomb blasts. This study aimed to evaluate DNA quality and quantity in adipose tissue affected by blasts compared to that in unaffected tissue. METHODS 10 adipose tissue samples were taken from regions near and far from the blast, representing the blast-exposed and non-blast-exposed groups. The adipose tissue was stored at a low temperature for 5 days, after which an organic extraction method was applied. The purity of the DNA extract was assessed using a NanoDrop spectrophotometer, and its integrity was evaluated using 0.8% concentration gel electrophoresis at 60 V for 90 min. DNA typing was conducted using the GlobalFiler™ kit, and DNA quantity was determined with the Quantifiler™ Trio DNA Quantification kit. RESULTS Of 20 DNA extracts from adipose tissue, all samples demonstrated purity, integrity, and complete typing results. Adequate integrity was found in 90% of samples in both groups. A 50% incidence of allele shifting was observed at the D7S820 locus within the blast-exposed group. CONCLUSIONS DNA from blast-exposed adipose tissue exhibited no significant quality or quantity differences from non-blast-exposed tissue. This suggested adipose tissue’s potential as an alternative DNA source in a bomb explosion.
Co-Authors Ahmad Yudianto Djarwani S. Soejoko Evi Untoro Herkutanto Kiagus Dahlan Leonardo Nurizati Nurizati Setia Utami Dewi Sugiharto, Ade Firmansyah Wahyu Widodo Windrianto, Mochamad Atmaji Wresti Indriatmi B. Makes Yessie Widya Sari Yuni, Mindya