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All Journal INDONESIAN JOURNAL OF LEGAL AND FORENSIC SCIENCES Jurnal Ilmu Kedokteran (Journal of Medical Science) Medical Journal of Indonesia Journal of the Indonesian Medical Association : Majalah Kedokteran Indonesia
Sugiharto, Ade Firmansyah
Departemen Forensik Dan Medikolegal, Rumah Sakit Cipto Mangunkusumo - Fakultas Kedokteran Universitas Indonesia
Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Education Health Professions Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Public Health

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Penentuan Umur Bercak Darah Manusia pada Kain Katun dengan Menggunakan High Performance Liquid Chromatography Mohammad Tegar Indrayana; Wibisana Widiatmaka; Ade Firmansyah Sugiharto; Ridla Bakri
Jurnal Ilmu Kedokteran Vol 8, No 1 (2014): Jurnal Ilmu Kedokteran
Publisher : Fakultas Kedokteran Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (107.923 KB) | DOI: 10.26891/JIK.v8i1.2014.8-17

Abstract

This research is to study the method in determining the age of human bloodstain due the rarity of human bloodstainresearches in Indonesia, by analyzing the X and Y peaks that appeared in High Performance Liquid Chromatography(HPLC). This research is experimental with time series for 11 days observation by exposing the bloodstain onto 33pieces of cotton fabric, each measuring 1 cm x 1 cm that originated from 3 respondents with environmental conditionsof the FKUI/RSUPN Dr. Cipto Mangunkusumo Forensic and Medico-legal Department which represents the area ofCentral Jakarta. The results of this research is the X and Y peaks do not point to a linearity so that the criteria for linearregression equation is not met. The conclusion of this research is age of human bloodstain on cotton fabric byanalyzing X peak with HPLC cannot yet be used pragmatically in the field in accord to the conditions at the crimescene.
Gambaran Patologis Hasil Autopsi Pada Leher Korban Homicidal Hanging Dan Suicidal Hanging Muhammad Ilham Dhiya Rakasiwi; Muhammad Taufik; Idham R. Dewantara; Yuli M. Shufiyani; Ade Firmansyah Sugiharto
Indonesian Journal of Legal and Forensic Sciences (IJLFS) Vol 11 No 2 (2021): Indonesian Journal of Legal and Forensic Sciences
Publisher : Penerbit, sejak 2012 : Asosiasi Ilmu Forensik Indonesia dan UPT Lab. Forensik Sain dan Kriminilogi - Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJLFS.2021.v11.i02.p04

Abstract

Gantung diri merupakan bentuk penyebab kematian di dunia. Gantung diri dapat terjadi karena bunuh diri, pembunuhan, kecelakaan atau hukuman. Telaah ini berfokus pada identifikasi jejas pada leher yang ditemukan pada kasus suicidal hanging dan homicidal hanging. Skirining literatur dilakukan pada 3 basis data Pubmed, Scopus dan Cochrane. Semua studi kasus dan laporan kasus berbahasa Inggris digunakan. Tidak ada batasan tahun. Setelah penelaahan kritis 10 laporan kasus homicidal hanging dan suicidal hanging, serta 7 studi kasus suicidal hanging. Telaah ini telah mengidentifikasi dan mensintesis bukti dari 17 studi homicidal hanging dan suicidal hanging. Jejas yang ditemukan berupa jejas jerat (jumlah, letak, arah), perdarahan otot, fraktur kartilago dan tanda kekerasan lain. Telaah ini dapat digunakan kalangan professional di bidang patologi forensik untuk memahami kasus homicidal hanging dan suicidal hanging, serta mengenalkan pentingnya studi literatur di bidang forensik.
Aspek Biomekanika Forensik Pada Trauma Peluru Plastik Airsoft Gun Sugiharto, Ade Firmansyah; Pattihahuan, Ira Zefanya
Majalah Kedokteran Indonesia Vol 75 No 1 (2025): Journal of The Indonesian Medical Association - Majalah Kedokteran Indonesia, Vo
Publisher : PENGURUS BESAR IKATAN DOKTER INDONESIA (PB IDI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47830/jinma-vol.75.1-2025-1854

Abstract

The biomechanical aspects of trauma caused by airsoft gun plastic pellets depend on the energy generated by the pellet and the tissue resistance. The airsoft gun plastic pellet creates lower energy compare to firearm, thus creating non-lethal effect to human body, but sufficient for creating tissue damage. This energy is determined by the velocity, weight, and diameter of the plastic pellet.. Indonesia allows for a maximum of 2 Joule energy, 8 mm in diameter, and 0,4 gram in maximum weight for airsoft gun plastic pellet. When it hits body surface, this characteristics will create energy of 3,98 J/cm2, and 7 J/cm2 with 6 mm plastic pellet diameter. The most common case of injury due to airsoft gun plastic pellet is eye ball injury. With a resistance of 4.3-4.8 J/cm², trauma from airsoft gun plastic pellets can result in corneal abrasion, hyphema, contusion, and even rupture.. Skin has much higher resistance, which is 23,99 J/cm2. Some injury may still be found, including erythema and 5 mm skin penetration.
DNA quality and quantity in adipose tissue: a comparison of the effects of bomb explosion Leonardo; Sugiharto, Ade Firmansyah; Indriatmi, Wresti; Atmadja, Djaja Surya; Yudianto, Ahmad; Herkutanto; Widodo, Wahyu
Medical Journal of Indonesia Vol. 32 No. 4 (2023): December
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13181/mji.oa.247206

Abstract

BACKGROUND Adipose tissue is often overlooked in DNA testing due to misconceptions about its DNA content. However, its shock-absorbing qualities may be useful for high-pressure scenarios like bomb blasts. This study aimed to evaluate DNA quality and quantity in adipose tissue affected by blasts compared to that in unaffected tissue. METHODS 10 adipose tissue samples were taken from regions near and far from the blast, representing the blast-exposed and non-blast-exposed groups. The adipose tissue was stored at a low temperature for 5 days, after which an organic extraction method was applied. The purity of the DNA extract was assessed using a NanoDrop spectrophotometer, and its integrity was evaluated using 0.8% concentration gel electrophoresis at 60 V for 90 min. DNA typing was conducted using the GlobalFiler™ kit, and DNA quantity was determined with the Quantifiler™ Trio DNA Quantification kit. RESULTS Of 20 DNA extracts from adipose tissue, all samples demonstrated purity, integrity, and complete typing results. Adequate integrity was found in 90% of samples in both groups. A 50% incidence of allele shifting was observed at the D7S820 locus within the blast-exposed group. CONCLUSIONS DNA from blast-exposed adipose tissue exhibited no significant quality or quantity differences from non-blast-exposed tissue. This suggested adipose tissue’s potential as an alternative DNA source in a bomb explosion.
Co-Authors Ahmad Yudianto Djaja Surya Atmadja Ganda, Michael Herkutanto Idham R. Dewantara Leonardo Mohammad Tegar Indrayana Muhammad Ilham Dhiya Rakasiwi Muhammad Taufik Pattihahuan, Ira Zefanya Ridla Bakri Wahyu Widodo Wibisana Widiatmaka Wresti Indriatmi B. Makes Yuli M. Shufiyani